秀士 藤本

細菌DNA分離におけるビーズ法の応用 (2004)

藤本, 秀士, 中上, 佳子, 小島, 夫美子, Fujimoto, Shuji, Nakagami, Yoshiko, Kojima, Fumiko

Extraction of nucleic acids from Gram-positive bacteria is normally hampered by a thick and resistant cell wall. This paper presents procedures based on mechanical cell breakage to extract DNA from...

スライド凝集法および免疫ドットブロット法による肺炎球菌莢膜型別の試み (2004)

藤本, 秀士, 小島, 夫美子, 中上, 佳子, 岩谷, 良則, 竹森, 紘一, Fujimoto, Shuji, ...

Streptococcus pneumoniae is the leading cause of community-acquired pneumonia, meningitis, and otitis media. In addition S. pneumoniae has been isolated from patients with conjunctivitis. In this...

墨汁染色変法による細菌莢膜の識別法の開発 (2004)

小島, 夫美子, 山田, 巌, 中上, 佳子, 岩谷, 良則, 藤本, 秀士, Kojima, Humiko, ...

The capsule of pathogenic bacteria is one of the most important virulence factors. Hiss's method, a standard bacterial capsule stain, is used for capsule identification. However it was difficult to...

眼脂から分離された肺炎球菌のペニシリン感受性について (2004)

小島, 夫美子, 竹森, 紘一, 東田, 正二, 中上, 佳子, 岩谷, 良則, 藤本, 秀士, ...

Recently, penicillin resistant Streptococcus pneumoniae (PRSP) is widespread all over the world. S. pneumoniae is the leading cause of community-acquired pneumonia, meningitis, and otitis media. In...

Random Amplified Polymorphic DNA(RAPD)解析による黄色ブドウ球菌ゲノムタイピング

藤本, 秀士, 小島, 夫美子

To perform the genotyping method of Staphylococcus aureus based on random amplified polymorphic DNA analysis (RAPD), we optimized the several polymerase chain reaction (PCR) conditions such as Mg2+...

健康な大学生における黄色ブドウ球菌の鼻腔内保菌状況とそのコアグラーゼ遺伝子型別

小島, 夫美子, 山田, 巌, 藤本, 秀士

Staphylococcus aureus commonly colonize in the human nasal cavity. Sometimes it causes food poisonings or nosocomial infections. We investigated the nasal carriage rate of S. aureus in a longitudinal...

リアルタイムPCRによる黄色ブドウ球菌表皮剥脱毒素遺伝子の検出

小島, 夫美子, 藤本, 秀士

Rapid detection of virulence factors of pathogens is important in diagnosis and treatment of infectious diseases. Certain strains of Staphylococcus aureus express either or both of two related, but...

Random Amplified Polymorphic DNA(RAPD)解析による黄色ブドウ球菌ゲノムタイピング

藤本, 秀士, 小島, 夫美子

To perform the genotyping method of Staphylococcus aureus based on random amplified polymorphic DNA analysis (RAPD), we optimized the several polymerase chain reaction (PCR) conditions such as Mg2+...

健康な大学生における黄色ブドウ球菌の鼻腔内保菌状況とそのコアグラーゼ遺伝子型別

小島, 夫美子, 山田, 巌, 藤本, 秀士

Staphylococcus aureus commonly colonize in the human nasal cavity. Sometimes it causes food poisonings or nosocomial infections. We investigated the nasal carriage rate of S. aureus in a longitudinal...

リアルタイムPCRによる黄色ブドウ球菌表皮剥脱毒素遺伝子の検出

小島, 夫美子, 藤本, 秀士

Rapid detection of virulence factors of pathogens is important in diagnosis and treatment of infectious diseases. Certain strains of Staphylococcus aureus express either or both of two related, but...