| Aphid Transmission and Buchnera sp. GroEL Affinity of a Potato leafroll virus RTD Deficient Mutant (2005) | |||||||||
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| Aphid Transmission and Buchnera GroEL Affinity Potato leafroll virus RTD Deficient Mutant Marcos Gon alves Frank van der Wilk Annette Dullemans Martin Verbeek Jorge Vega Johannes van den Heuvel Instituto Biol gico CPDSV Conselheiro Rodrigues Alves CEP Paulo Brazil mail mcgon biologico gov COGEM Commission Genetic Modification Box Bilthoven The Netherlands Plant Research International O Box Wageningen The Netherlands Universidade Estadual Campinas Postal CEP Campinas Brazil Ruiter Seeds Box Bergschenhoek The Netherlands Aceito para publica Corresponding author Marcos Gon alves GON ALVES AN DER WILK DULLEMANS VERBEEK VEGA DEN HEUVEL M Aphid transmission and Buchnera GroEL affinity Potato leafroll virus RTD deficient mutant Fitopatologia Brasileira ABSTRACT Potato leafroll virus PLRV genus Polerovirus family Luteoviridae transmitted aphids persistent and circulative manner Members the family Luteoviridae associate with GroEL homologue produced the primary endosymbiont Buchnera aphids avoid degradation the hemolymph Purified luteovirus particles contain two types proteins major capsid protein kDa and minor capsid component kDa which truncated form translation read through protein the gene termination codon The read through domain RTD contains determinants responsible for virus transmission infectious full length cDNA clone PLRV and mutant devoid the RTD were used analyze the molecular interactions between this luteovirus and its aphid vector Myzus persicae The PLRV mutant virions | |||||||||
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