Publikationsansicht

Focus on function: Single molecule RNA enzymology

Abstract

The ability of RNA to catalyze chemical reactions was first demonstrated 25 years ago with the discovery that group I introns and RNase P function as RNA enzymes (ribozymes). Several additional ribozymes were subsequently identified, most notably the ribosome, followed by intense mechanistic studies. More recently, the introduction of single molecule tools has dissected the kinetic steps of several ribozymes in unprecedented detail and has revealed surprising heterogeneity not evident from ensemble approaches. Still, many fundamental questions of how RNA enzymes work at the molecular level remain unanswered. This review surveys the current status of our understanding of RNA catalysis at the single molecule level and discusses the existing challenges and opportunities in developing suitable assays. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 302–316, 2007. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/57325/1/20819_ftp.pdf

Details der Publikation

Download	, http://hdl.handle.net/2027.42/57325 http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17685395&dopt=citation http://dx.doi.org/10.1002/bip.20819
Herausgeber	Wiley Subscription Services, Inc., A Wiley Company
Mitarbeiter	Biophysics Research Division, Single Molecule Analysis Group, University of Michigan, Ann Arbor, MI 48109, Department of Chemistry, Single Molecule Analysis Group, University of Michigan, Ann Arbor, MI 48109 ; Department of Chemistry, Single Molecule Analysis Group, University of Michigan, Ann Arbor, MI 48109, Department of Chemistry, Wayne State University, Detroit, MI 48202, Department of Chemistry, Wayne State University, Detroit, MI 48202 ; Department of Chemistry, Wayne State University, Detroit, MI 48202Department of Chemistry, Wayne State University, Detroit, MI 48202
Archiv	University of Michigan (United States)
Keywords	Chemistry, Polymer and Materials Science, Chemical Engineering, Chemistry, Materials Science and Engineering, Engineering, Science