| Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations (2007) | |||||||||||||||
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| Complementary DNA (cDNA)-amplified fragment-length polymorphism (AFLP) was applied to analyze transcript profiles of a Zn-tolerant and a Zn-sensitive isolate of the ectomycorrhizal basidiomycete Suillus luteus, both cultured with and without increased external zinc concentrations. From the obtained transcript profiles that covered approximately 2% of the total expected complement of genes in S. luteus, 144 nonredundant, differentially expressed transcript-derived fragments (TDFs), falling in different classes of expression pattern, were isolated and sequenced. Thirty-six of the represented genes showed homology to function-known genes, whereas 6 matched unknown protein coding sequences, and 102 were possibly novel. Although relatively few TDFs were found to be responsive to the different zinc treatments, their modulated expression levels may suggest a different transcriptional response to zinc treatments in both isolates. Among the identified genes that could be related to heavy-metal detoxification or the tolerance trait were genes encoding for homologues of a heat-shock protein, a putative metal transporter, a hydrophobin, and several proteins involved in ubiquitin-dependent proteolysis.. Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. Univ Libre Bruxelles, Lab Physiol & Genet Mol Plantes, B-1050 Brussels, Belgium. Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA.COLPAERT, JV, Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, Gebouw D, B-3590 Diepenbeek, Belgium.jan.colpaert@uhasselt.be | |||||||||||||||
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