| Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-{gamma} 2a heavy chain (1980) | |||||||||||||
Abstract | |||||||||||||
| A DNA sequence complementary to the entire coding part of a mouse γ2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the PstI site of pBR 322. Transformants containing sequences complementary to purified γ2a heavy chain mRNA were selected. One transformant, pG2a-10–21, containing a 1750 nucleotide insert, has been characterized by hybrid-arrested translation and purification of γ2a heavy chain mRNA on DNA-DBM cellulose filters. Restriction enzyme analysis and partial sequencing demonstrate that the pG2a-10–21 contains the complete structural sequence for the γ2a heavy chain and predicts the sequence of a 18 amino acid hydrophobic amino terminal extra piece segment. | |||||||||||||
Details der Publikation | |||||||||||||
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