| On the efficacy of commonly used ribonuclease inhibitors (1976) | |||||||||||||
Abstract | |||||||||||||
| The ability of a number of commonly used inhibitors to inhibit pancreatic ribonuclease has been studied. At ribonuclease concentrations of 10 or 100 g/ml, heparin, polyvinylsulfate and proteinase K, at concentrations reported for their use in the literature, were ineffective in inhibiting RNase digestion of 3H-uridine labelled RNA from . In contrast, macaloid, diethylpyrocarbonate and sodium dodecyl sulfate were all effective inhibitors, with the degree of effectiveness decreasing in the order stated. Further, at inhibitor concentrations which allowed RNase conversion of only 50% of the labelled RNA to acid soluble products, a larger percentage of the acid insoluble digestion products sedimented in the "high molecular weight" range (4-16s) when macaloid was the inhibitor used than when diethylpyrocarbonate was the inhibitor.. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/21807/1/0000207.pdf | |||||||||||||
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