| Sensitivity of transcription by purified RNA polymerase to actinomycin (1978) | |||||||||||||
Abstract | |||||||||||||
| DNA-dependent RNA polymerase has been purified 400-fold from 48 hr old, actinomycin producing cells of . Sodium dodecyl sulfate polyacrylamide gel electrophoresis of the purified polymerase reveals the presence of bands corresponding to the [beta], [beta]', and [alpha] subunits of polymerase, but no [sigma] subunit. The purified polymerase is also associated with a protein component of 145,000 daltons and it is suggested that this protein is produced by limited proteolysis of either the [beta] or [beta]' subunits. "In vitro" studies have shown that transcription catalyzed by either or RNA polymerase is inhibited to about the same extent by actinomycin.. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/22507/1/0000051.pdf | |||||||||||||
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