| Molecular cloning and in vitro expression of a silent phenoxazinone synthase gene from Streptomyces lividans (1989) | |||||||||||||
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| Phenoxazinone synthase (PHS) catalyzes a step in actinomycin D biosynthesis in Streptomyces antibioticus. Two sequences from Streptomyces lividans that hybridize to the phs gene of S. antibioticus have been cloned in Escherichia coli K-12 using the plasmid pBR322. Although there was some similarity in the restriction maps of the two cloned fragments, neither insert appeared to be a direct subset of the other nor of the S. antibioticus phs gene. In vitro expression studies, in a streptomycete coupled transcription-translation system, showed that a 3.98-kb SphI fragment encoded a PHS-related protein. These observations provide additional support for the existence of silent genes for antibiotic production in streptomycetes.. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/27637/1/0000013.pdf | |||||||||||||
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