Kalamaki, M.S., Powell, A.L.T., Struijs, K., Labavitch, J.M., Reid, D.S., Bennett, A.B.
Suppression of the expression of a ripening-related expansin gene, LeExp1, in tomato enhanced fruit firmness and overexpression of LeExp1 resulted in increased fruit softening. Because of the...
In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries.
Karrer, E E, Lincoln, J E, Hogenhout, S, Bennett, A B, Bostock, R M, Martineau, B, ...
A method for isolating and cloning mRNA populations from individual cells in living, intact plant tissues is described. The contents of individual cells were aspirated into micropipette tips filled...
Higher plant Ca(2+)-ATPase: primary structure and regulation of mRNA abundance by salt.
Wimmers, L E, Ewing, N N, Bennett, A B
Calcium-dependent regulatory mechanisms participate in diverse developmentally, hormonally, and environmentally regulated processes, with the precise control of cytosolic Ca2+ concentration being...
Ascorbate free radical reductase mRNA levels are induced by wounding.
Grantz, A A, Brummell, D A, Bennett, A B
A cDNA clone encoding ascorbate free radical (AFR) reductase (EC 1.6.5.4) was isolated from tomato (Lycopersicon esculentum Mill.) and its mRNA levels were analyzed. The cDNA encoded a deduced...
The effect of a tomato (Lycopersicon esculentum) mutation, diageotropica (dgt), on the accumulation of mRNA corresponding to tomato homologs of three auxin-regulated genes, LeAux, LeSAUR, and Lepar,...
Rose, J K, Brummell, D A, Bennett, A B
A cDNA encoding a xyloglucan endotransglycosylase (XET) homolog was isolated from nasturtium (Tropaeolum majus) epicotyl RNA. The deduced protein encoded by the corresponding gene, termed XET1, was...
Pedicel breakstrength and cellulase gene expression during tomato flower abscission.
Six cellulase genes were isolated from total RNA of the ethylene-treated tomato (Lycopersicon esculentum Mill.) flower abscission zone by reverse-transcription polymerase chain reaction using...
Sugar regulates mRNA abundance of H(+)-ATPase gene family members in tomato.
Mito, N, Wimmers, L E, Bennett, A B
The plant plasma membrane H(+)-ATPase energizes the secondary uptake of nutrients and may facilitate cell expansion by acidifying the cell wall. In yeast, Glc stimulates the accumulation of...
Klann, E M, Hall, B, Bennett, A B
Invertase (beta-fructosidase, EC 3.2.1.26) hydrolyzes sucrose to hexose sugars and thus plays a fundamental role in the energy requirements for plant growth and maintenance. Transgenic plants with...
Divergent fructokinase genes are differentially expressed in tomato.
Kanayama, Y, Dai, N, Granot, D, Petreikov, M, Schaffer, A, Bennett, A B
Two cDNA clones (Frk1 and Frk2) encoding fructokinase (EC 2.7.1.4) were isolated from tomato (Lycopersicon esculentum). The Frk2 cDNA encoded a deduced protein of 328 amino acids that was more than...
Gonzalez-Bosch, C, Del Campillo, E, Bennett, A B
Tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase Cel1 mRNA accumulation was previously correlated with abscission of flower explants. Cel1 antibodies were raised against a fusion...
Molecular characterization of a polygalacturonase inhibitor from Pyrus communis L. cv Bartlett.
Stotz, H U, Powell, A L, Damon, S E, Greve, L C, Bennett, A B, Labavitch, J M
A polygalacturonase inhibitor glycoprotein with an apparent molecular mass of 43 kD was purified from pear (Pyrus communis L. cv Bartlett) fruit. Chemical deglycosylation of this protein decreased...
Expression of Acid Invertase Gene Controls Sugar Composition in Tomato (Lycopersicon) Fruit.
Klann, E. M., Chetelat, R. T., Bennett, A. B.
A wild tomato species, Lycopersicon chmielewskii, accumulates high levels of soluble sugar in mature fruit and, unlike the domesticated tomato species, Lycopersicon esculentum, accumulates sucrose...
Assessment of the number and expression of P-type H(+)-ATPase genes in tomato.
Seven genomic fragments encoding isoforms of tomato (Lycopersicon esculentum) plasma membrane H(+)-ATPase were cloned and characterized. Genomic DNA gel-blot analysis indicated that probes...
Polygalacturonase isozyme 1 (PG1) is a heterodimer comprising a catalytic and noncatalytic or [beta] subunit, whereas polygalacturonase isozyme 2 (PG2) comprises only the catalytic subunit. To assess...
Giovannoni, J J, DellaPenna, D, Bennett, A B, Fischer, R L
Tomato fruit ripening is accompanied by extensive degradation of pectic cell wall components. This is thought to be due to the action of a single enzyme, polygalacturonase, whose activity is...
Analysis of tomato polygalacturonase expression in transgenic tobacco.
Osteryoung, K W, Toenjes, K, Hall, B, Winkler, V, Bennett, A B
Tomato polygalacturonase is a cell wall enzyme secreted in large amounts during tomato fruit ripening. Polygalacturonase is synthesized as a glycoprotein precursor that undergoes numerous...
Lashbrook, C C, Gonzalez-Bosch, C, Bennett, A B
Two structurally divergent endo-beta-1,4-glucanase (EGase) cDNAs were cloned from tomato. Although both cDNAs (Cel1 and Cel2) encode potentially glycosylated, basic proteins of 51 to 53 kD and...
Gonzalez-Bosch, C., Brummell, D. A., Bennett, A. B.
The mRNA accumulation of two endo-1,4-[beta]-D-glucanase genes, Cel1 and Cel2, was examined in the pericarp and locules throughout the development of normal tomato (Lycopersicon esculentum) fruit and...
Reuveni, M., Bennett, A. B., Bressan, R. A., Hasegawa, P. M.
Tonoplast enriched membrane vesicle fractions were isolated from unadapted and NaCl (428 millimolar) adapted tobacco cells (Nicotiana tabacum L. var Wisconsin 38). Polypeptides from the tonoplast...
In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries.
Karrer, E E, Lincoln, J E, Hogenhout, S, Bennett, A B, Bostock, R M, Martineau, B, ...
A method for isolating and cloning mRNA populations from individual cells in living, intact plant tissues is described. The contents of individual cells were aspirated into micropipette tips filled...
Higher plant Ca(2+)-ATPase: primary structure and regulation of mRNA abundance by salt.
Wimmers, L E, Ewing, N N, Bennett, A B
Calcium-dependent regulatory mechanisms participate in diverse developmentally, hormonally, and environmentally regulated processes, with the precise control of cytosolic Ca2+ concentration being...
Ascorbate free radical reductase mRNA levels are induced by wounding.
Grantz, A A, Brummell, D A, Bennett, A B
A cDNA clone encoding ascorbate free radical (AFR) reductase (EC 1.6.5.4) was isolated from tomato (Lycopersicon esculentum Mill.) and its mRNA levels were analyzed. The cDNA encoded a deduced...
The effect of a tomato (Lycopersicon esculentum) mutation, diageotropica (dgt), on the accumulation of mRNA corresponding to tomato homologs of three auxin-regulated genes, LeAux, LeSAUR, and Lepar,...
Rose, J K, Brummell, D A, Bennett, A B
A cDNA encoding a xyloglucan endotransglycosylase (XET) homolog was isolated from nasturtium (Tropaeolum majus) epicotyl RNA. The deduced protein encoded by the corresponding gene, termed XET1, was...
Pedicel breakstrength and cellulase gene expression during tomato flower abscission.
Six cellulase genes were isolated from total RNA of the ethylene-treated tomato (Lycopersicon esculentum Mill.) flower abscission zone by reverse-transcription polymerase chain reaction using...
Sugar regulates mRNA abundance of H(+)-ATPase gene family members in tomato.
Mito, N, Wimmers, L E, Bennett, A B
The plant plasma membrane H(+)-ATPase energizes the secondary uptake of nutrients and may facilitate cell expansion by acidifying the cell wall. In yeast, Glc stimulates the accumulation of...
Klann, E M, Hall, B, Bennett, A B
Invertase (beta-fructosidase, EC 3.2.1.26) hydrolyzes sucrose to hexose sugars and thus plays a fundamental role in the energy requirements for plant growth and maintenance. Transgenic plants with...
Divergent fructokinase genes are differentially expressed in tomato.
Kanayama, Y, Dai, N, Granot, D, Petreikov, M, Schaffer, A, Bennett, A B
Two cDNA clones (Frk1 and Frk2) encoding fructokinase (EC 2.7.1.4) were isolated from tomato (Lycopersicon esculentum). The Frk2 cDNA encoded a deduced protein of 328 amino acids that was more than...
Gonzalez-Bosch, C, Del Campillo, E, Bennett, A B
Tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase Cel1 mRNA accumulation was previously correlated with abscission of flower explants. Cel1 antibodies were raised against a fusion...
Molecular characterization of a polygalacturonase inhibitor from Pyrus communis L. cv Bartlett.
Stotz, H U, Powell, A L, Damon, S E, Greve, L C, Bennett, A B, Labavitch, J M
A polygalacturonase inhibitor glycoprotein with an apparent molecular mass of 43 kD was purified from pear (Pyrus communis L. cv Bartlett) fruit. Chemical deglycosylation of this protein decreased...
Expression of Acid Invertase Gene Controls Sugar Composition in Tomato (Lycopersicon) Fruit.
Klann, E. M., Chetelat, R. T., Bennett, A. B.
A wild tomato species, Lycopersicon chmielewskii, accumulates high levels of soluble sugar in mature fruit and, unlike the domesticated tomato species, Lycopersicon esculentum, accumulates sucrose...
Assessment of the number and expression of P-type H(+)-ATPase genes in tomato.
Seven genomic fragments encoding isoforms of tomato (Lycopersicon esculentum) plasma membrane H(+)-ATPase were cloned and characterized. Genomic DNA gel-blot analysis indicated that probes...
Polygalacturonase isozyme 1 (PG1) is a heterodimer comprising a catalytic and noncatalytic or [beta] subunit, whereas polygalacturonase isozyme 2 (PG2) comprises only the catalytic subunit. To assess...
Giovannoni, J J, DellaPenna, D, Bennett, A B, Fischer, R L
Tomato fruit ripening is accompanied by extensive degradation of pectic cell wall components. This is thought to be due to the action of a single enzyme, polygalacturonase, whose activity is...
Analysis of tomato polygalacturonase expression in transgenic tobacco.
Osteryoung, K W, Toenjes, K, Hall, B, Winkler, V, Bennett, A B
Tomato polygalacturonase is a cell wall enzyme secreted in large amounts during tomato fruit ripening. Polygalacturonase is synthesized as a glycoprotein precursor that undergoes numerous...
Lashbrook, C C, Gonzalez-Bosch, C, Bennett, A B
Two structurally divergent endo-beta-1,4-glucanase (EGase) cDNAs were cloned from tomato. Although both cDNAs (Cel1 and Cel2) encode potentially glycosylated, basic proteins of 51 to 53 kD and...
Gonzalez-Bosch, C., Brummell, D. A., Bennett, A. B.
The mRNA accumulation of two endo-1,4-[beta]-D-glucanase genes, Cel1 and Cel2, was examined in the pericarp and locules throughout the development of normal tomato (Lycopersicon esculentum) fruit and...
Reuveni, M., Bennett, A. B., Bressan, R. A., Hasegawa, P. M.
Tonoplast enriched membrane vesicle fractions were isolated from unadapted and NaCl (428 millimolar) adapted tobacco cells (Nicotiana tabacum L. var Wisconsin 38). Polypeptides from the tonoplast...
Cantu, D., Vicente, A. R., Greve, L. C., Dewey, F. M., Bennett, A. B., Labavitch, J. M., ...
Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the...