A. I. Azuaga

Comparative NMR study on the impact of point mutations on protein stability of Pseudomonas mendocina lipase (2006)

Sibille, N., Favier, A., Azuaga, A.I., Ganshaw, G., Bott, R., Bonvin, A.M.J.J., ...

In this work we compare the dynamics and conformational stability of Pseudomonas mendocina lipase enzyme and its F180P/S205G mutant that shows higher activity and stability for use in washing...

Characterization of single-tryptophan mutants of histidine-containing phosphocarrier protein: evidence for local rearrangements during folding from high concentrations of denaturant (2003)

Azuaga, A.I., Canet, D., Smeenk, G., Berends, R., Titgemeijer, F., Duurkens, R., ...

We have used site-directed mutagenesis in combination with a battery of biophysical techniques to probe the stability and folding behavior of a small globular protein, the histidine-containing...

Analysis of the interactions between streptokinase domains and human plasminogen (1998)

CONEJERO-LARA, F., PARRADO, J., AZUAGA, A. I., DOBSON, C. M., PONTING, C. P.

Thermal stability of the three domains of streptokinase studied by circular dichroism and nuclear magnetic resonance (1996)

CONEJERO-LARA, F., PARRADO, J., AZUAGA, A. I., SMITH, RAG., PONTING, C. P., DOBSON, C. M.

Thermal stability of the three domains of streptokinase studied by circular dichroism and nuclear magnetic resonance.

Conejero-Lara, F., Parrado, J., Azuaga, A. I., Smith, R. A., Ponting, C. P., Dobson, C. M.

Streptococcus equisimilis streptokinase (SK) is a single-chain protein of 414 residues that is used extensively in the clinical treatment of acute myocardial infarction due to its ability to activate...

Analysis of the interactions between streptokinase domains and human plasminogen.

Conejero-Lara, F., Parrado, J., Azuaga, A. I., Dobson, C. M., Ponting, C. P.

The contrasting roles of streptokinase (SK) domains in binding human Glu1-plasminogen (Plg) have been studied using a set of proteolytic fragments, each of which encompasses one or more of SK's three...

Expression and characterization of the intact N-terminal domain of streptokinase.

Azuaga, A. I., Woodruff, N. D., Conejero-Lara, F., Cox, V. F., Smith, R. A., Dobson, C. M.

Proteolytic studies have enabled two of the three putative domains of the fibrinolytic protein streptokinase to be isolated and characterized (Conejero-Lara F et al., 1996, Protein Sci 5:2583-2591)....