A. Kimura

Details der Publikationsliste

Zeitraum

1988 - 2010

Anzahl

136

Co-Autoren

Superdeformation in Asymmetric N$>$Z Nucleus $^{40}$Ar (2009)

Ideguchi, E., Ota, S., Morikawa, T., Oshima, M., Koizumi, M., Toh, Y., ...

A rotational band with five $\gamma$-ray transitions ranging from 2$^{+}$ to 12$^{+}$ states was identified in $^{40}$Ar. This band is linked through $\gamma$ transitions from the excited 2$^{+}$,...

Identification of an imidazoline binding protein: Creatine kinase and an imidazoline-2 binding site (2009)

Kimura, A., Tyacke, R. J., Robinson, J J, Husbands, Stephen M, Minchin, M. C. W., Nutt, D. J., ...

Drugs that bind to imidazoline binding proteins have major physiological actions. To date, three subtypes of such proteins, I-1, I-2 and I-3, have been proposed, although characterisations of these...

Electronic Structures of Fe$_{3-x}V$_x$Si Probed by Photoemission Spectroscopy (2008)

Cui, Y. T., Kimura, A., Miyamoto, K., Sakamoto, K., Xie, T., Qiao, S., ...

The electronic structures of the Heusler type compounds Fe$_{3-x}V$_x$Si in the concentration range between x = 0 and x = 1 have been probed by photoemission spectroscopy (PES). The observed shift of...

IRRADIATION EMBRITTLEMENT OF CLADDING AND HAZ OF RPV STEEL (2008)

Lee, J.S., Kim, I.S., Jang, C.H., Kimura, A.

Microstructural features and their related mechanical property changes in the 309L cladding and the heat affected zone (HAZ) of SA508 cl.3 steel were investigated through the use of TEM, tensile and...

FUSION MATERIALS AND FUSION ENGINEERING R&D IN JAPAN (2008)

KOHYAMA, A., KONISHI, S., KIMURA, A.

Japanese activities on fusion structural materials R&D have been well organized under the coordination of university programs and JAERI/NIMS programs more than two decades. Where, two categories of...

Laminin-alpha4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and endothelial cells (2007)

Knoll, R., Postel, R., Wang, J., Kratzner, R., Hennecke, G., Vacaru, A. M., ...

BACKGROUND: Extracellular matrix proteins, such as laminins, and endothelial cells are known to influence cardiomyocyte performance; however, the underlying molecular mechanisms remain poorly...

Status of reduced activation ferritic/martensitic steel development (2007)

Baluc, N., Gelles, D. S., Jitsukawa, S., Kimura, A., Klueh, R. L., Odette, G. R., ...

Recent research results obtained in Europe, Japan, China and the USA on reduced activation ferritic/martensitic (RAFM) steels are reviewed. The present status of different RAFM steel products (plate,...

Embrittlement and hardening during thermal aging of high Cr oxide dispersion strengthened alloys (2007)

Lee, J.S., Jang, C.H., Kim, I.S., Kimura, A.

The effects of thermal aging on the microstructure and mechanical property changes in high Cr oxide dispersion strengthened (ODS) steels were investigated by using TEM, microhardness and small punch...

Corrosion behavior of Al-alloying high Cr-ODS steels in lead-bismuth eutectic. (2007)

Takaya, S., Furukawa, T., Aoto, K., Müller, G., Weisenburger, A., Heinzel, A., ...

13th Internat.Conf.on Fusion Reactor Materials (ICFRM-13), Nice, F, December 10-14, 2007

Spin-polarized surface state of MnSb(0001) (2005)

Rader, O., Lezaic, M., Blügel, S., Fujimori, A., Kimura, A., Kamakura, N., ...

This version is available at the following Publisher URL: http://stacks.iop.org/1367-2630/7/111

Current status and future prospects of ODS steel development for fusion. (2005)

Lindau, R., Hoelzer, D.T., Odette, G.R., Kimura, A., Ukai, S.

12th Internat.Conf.on Fusion Reactor Materials (ICFRM-12), Santa Barbara, Calif., December 4-9, 2005

Measuring Aqueous Humor Glucose Across Physiological Levels: NIR Raman Spectroscopy, Multivariate Analysis, Artificial Neural Networks, and Bayesian Probabilities (2004)

Storrie-Lombardi, M., Lambert, J., Borchert, M., Kimura, A., Roseto, J., Bing, R.

We have elicited a reliable Raman spectral signature for glucose in rabbit aqueous humor across mammalian physiological ranges in a rabbit model stressed by recent myocardial infarction.

Electronic structures of Cr$_{1-\delta}$X (X=S, Te) studied by Cr 2p soft x-ray magnetic circular dichroism (2004)

Yaji, K., Kimura, A., Hirai, C., Taniguchi, M., Koyama, M., Sato, H., ...

Cr 2p core excited XAS and XMCD spectra of ferromagnetic Cr$_{1-\delta}$Te with several concentrations of $\delta$=0.11-0.33 and ferrimagnetic Cr$_{5}$S$_{6}$ have been measured. The observed XMCD...

High quality operation of gyrotron aiming toward the convenient radiation source in the submillimeter wave length range. (2004)

Ogawa, I., Idehara, T., Sasagawa, H., Kimura, A., Mitsudo, S., Hori, T., ...

Displays and Vacuum Electronics : Proc.of the 10th Triennial ITG-Conf., Garmisch-Partenkirchen, May 3-4, 2004

Fundamental performance of flux-lock type fault current limiter with two air-core coils (2003)

Matsumura, T., Kimura, A., Shimizu, H., Yokomizu, Y., Goto, M.

This paper proposes a superconducting fault current limiter (FCL) which is a modified version of the flux-lock type fault current limiter developed by us. This FCL consists of a high Tc...

Soft X-ray Magnetic Circular Dichroism of c(2x2) CuMn Ordered Surface Alloy (2003)

Kimura, A., Kanbe, T., Xie, T., Taniguchi, M., Qiao, S., Muro, T., ...

Mn 2p soft X-ray absorption (XAS) spectroscopy excited with circularly polarized synchrotron radiation has been applied to a new class of material, c(2x2)CuMn/Cu(001) two-dimensional ordered surface...

Soft X-ray magnetic circular dichroism study of the ferromagnetic Cr$_{1-\delta}$Te (2003)

Yaji, K., Kimura, A., Hirai, C., Sato, H., Taniguchi, M., Koyama, M., ...

The 2p core excited XAS and XMCD spectra of Cr$_{1-\delta}$Te with several concentrations of $\delta$=0.11-0.33 have been measured. The observed XMCD lineshapes are found to very weakly depend on...

Atomic correlations in itinerant ferromagnets: quasi-particle bands of nickel (2002)

Buenemann, J., Gebhard, F., Ohm, T., Umstaetter, R., Weiser, S., Weber, W., ...

We measure the band structure of nickel along various high-symmetry lines of the bulk Brillouin zone with angle-resolved photoelectron spectroscopy. The Gutzwiller theory for a nine-band Hubbard...

Yamato 792947, 793408 and 82038: The most primitive H chondrites, with abundant refractory inclusions (2002)

Kimura, A., Hiyagon, H., Palme, H., Spettel, B., Wolf, D., Clayton, R. N., ...

In this paper we report petrological and chemical data of the unusual chondritic meteorites Yamato (Y)-792947, Y-93408 and Y- 82038. The three meteorites are very similar in texture and chemical...

Ferritic/martensitic steels - overview of recent results (2002)

Klueh, R. L., Gelles, D. S., Jitsukawa, S., Kimura, A., Odette, G. R., Van Der Schaaf, B., ...

Considerable research work has been conducted on the ferritic/martensitic steels since the last International Conference on Fusion Reactor Materials in 1999. Since only a limited amount of that work...

Development of an extensive database of mechanical and physical properties for reduced-activation martensitic steel F82H. (2002)

Jitsukawa, S., Tamura, M., Van Der Schaaf, B., Klueh, R.L., Alamo, A., Petersen, C., ...

10th Internat.Conf.on Fusion Reactor Materials (ICFRM-10), Baden-Baden, October 14-19, 2001

Progress and critical issues of reduced activation ferritic/martensitic steel development. (2000)

Van Der Schaaf, B., Gelles, D.S., Jitsukawa, S., Kimura, A., Klueh, R.L., Möslang, A., ...

9th Internat.Conf.on Fusion Reactor Materials (ICFRM-9), Colorado Springs, Colo., October 10-15, 1999

Anomalous Strain Rate Dependence of the Serrated Flow in Ni - H and Ni - C - H Alloys. (1998)

Kimura, A., Birnbaum, H. K.

Serrated flow in Ni-H, Ni-C-H, and Ni-C alloys was studied over a wide range of temperature and strain rates. The results for C related serrated flow in Ni-C or Ni-C-H alloys were in excellent...

Effect of Adsorbed Arsenic on the Decomposition Rate of Nickel Hydride. (1998)

Kimura, A., Birnbaum, H. K.

The decomposition rate of nickel hydride was studied using x-ray, Auger spectroscopy, and SIMS techniques. In high purity nickel, which was cathodically charged with hydrogen, deposition of arsenic...

Effects of Small Changes in Alloy Composition on the Mechanical Properties of Low Activation 9%Cr-2%W Steel. (1998)

Kayano, H., Kimura, A., Narui, M., Kikuchi, T., Ohta, S.

9Cr-2W steels with small additions of alloying elements were creep and tensile tested to determine the effects of B, Mn, Ta, Ti, Zr, Al and Y; an increase in the amount of Ti, Mn and Ta and a partial...

Correlation satellite driven by reduced dimensionality (1997)

Rader, O, Vescovo, E, Wuttig, M, Sarma, DD, Blugel, S, Himpsel, FJ, ...

Photoelectron spectra of the ordered surface alloy c(2 x 2) CuMn/Cu(100) show distinct valence band spectral features at higher binding energy, which are most pronounced for the optimal CuMn c(2 x 2)...

Atrial conduction abnormalities in patients with systemic progressive sclerosis (1997)

Mizuno, R., Fujimoto, S., Nakano, H., Nakajima, T., Kimura, A., Nakagawa, Y., ...

Background Atrial abnormalities in patients with progressive systemic sclerosis have not been evaluated in terms of intra-artrial conduction. We hypothesized that a delay in atrial conduction in...

The Cherenkov Correlated Timing Detector: Beam Test Results from Quartz and Acrylic Bars (1995)

Kichimi, H., Sugaya, Y., Yamaguchi, H., Yoshimura, Y., Kanda, S., Olsen, S., ...

Several prototypes of a Cherenkov Correlated Timing (CCT) Detector have been tested at the KEK-PS test beam line. We describe the results for Cherenkov light yields and timing characteristics from...

HYDROGEN SEGREGATION AND DIFFUSION AT GRAIN BOUNDARIES (1988)

Birnbaum, H., Ladna, B., Kimura, A.

The segregation of hydrogen at grain boundaries in Ni has been shown to be dependent on the nature of the boundary using SIMS techniques. This contrasts to hydrogen segregation at free surfaces which...

HYDROGEN SEGREGATION AND DIFFUSION AT GRAIN BOUNDARIES (1988)

Birnbaum, H., Ladna, B., Kimura, A.

The segregation of hydrogen at grain boundaries in Ni has been shown to be dependent on the nature of the boundary using SIMS techniques. This contrasts to hydrogen segregation at free surfaces which...

HYDROGEN SEGREGATION AND DIFFUSION AT GRAIN BOUNDARIES (1988)

Birnbaum, H., Ladna, B., Kimura, A.

The segregation of hydrogen at grain boundaries in Ni has been shown to be dependent on the nature of the boundary using SIMS techniques. This contrasts to hydrogen segregation at free surfaces which...

HYDROGEN SEGREGATION AND DIFFUSION AT GRAIN BOUNDARIES (1988)

Birnbaum, H., Ladna, B., Kimura, A.

The segregation of hydrogen at grain boundaries in Ni has been shown to be dependent on the nature of the boundary using SIMS techniques. This contrasts to hydrogen segregation at free surfaces which...

HYDROGEN SEGREGATION AND DIFFUSION AT GRAIN BOUNDARIES (1988)

Birnbaum, H., Ladna, B., Kimura, A.

The segregation of hydrogen at grain boundaries in Ni has been shown to be dependent on the nature of the boundary using SIMS techniques. This contrasts to hydrogen segregation at free surfaces which...

Cloning and expression of a putative alcohol dehydrogenase gene of Entamoeba histolytica and its application to immunological examination.

Kimura, A, Hara, Y, Kimoto, T, Okuno, Y, Minekawa, Y, Nakabayashi, T

To clone and express the genes encoding major antigens of Entamoeba histolytica, we constructed a lambda gt11 cDNA library for E. histolytica HM1:IMSS and screened it with pooled sera from patients...

Modification of Escherichia coli B glutathione synthetase with polyethylene glycol for clinical application to enzyme replacement therapy for glutathione deficiency.

Inoue, Y, Sugiyama, K, Ueminami, H, Izawa, S, Kimura, A

Glutathione synthetase of Escherichia coli B was modified with polyethylene glycol, and the properties of the resultant modified enzyme were investigated. The thermal stability of the modified enzyme...

Purification and characterization of a novel nucleoside phosphorylase from a Klebsiella sp. and its use in the enzymatic production of adenine arabinoside.

Ling, F, Inoue, Y, Kimura, A

An adenosine-assimilating bacterium, Klebsiella sp. strain LF1202, inducibly formed a novel nucleoside phosphorylase which acted on both purine and pyrimidine nucleosides when the cells were cultured...

Role of human major histocompatibility complex DQ molecules in superantigenicity of streptococcus-derived protein.

Esaki, Y, Fukui, Y, Sudo, T, Yamamoto, K, Inamitsu, T, Nishimura, Y, ...

Antigenicity of peptic extract from type 12 group A streptococci (PEAST12) for T cells was examined in major histocompatibility complex (MHC) class II transgenic mice. PEAST12 was mitogenic for...

A microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity.

Murata, K, Higaki, N, Kimura, A

Enterobacter aerogenes IFO 12010 contains a carbon-phosphorus (C-P) bond cleavage enzyme catalyzing the liberation of inorganic phosphate from various alkyl- and phenylphosphonic acids. The enzyme in...

Purification and some properties of glutathione S-transferase from Escherichia coli B.

Iizuka, M, Inoue, Y, Murata, K, Kimura, A

Glutathione S-transferase was purified approximately 2,300-fold from cell extracts of Escherichia coli B with a 7.5% activity yield. The molecular weight of the enzyme was 45,000, and the enzyme...

Cystathionine accumulation in Saccharomyces cerevisiae.

Ono, B, Suruga, T, Yamamoto, M, Yamamoto, S, Murata, K, Kimura, A, ...

A cysteine-dependent strain of Saccharomyces cerevisiae and its prototrophic revertants accumulated cystathionine in cells. The cystathionine accumulation was caused by a single mutation having a...

Purine-mediated growth inhibition caused by a pyrE mutation in Escherichia coli K-12.

Shimosaka, M, Fukuda, Y, Murata, K, Kimura, A

A purine-sensitive phenotype results from a previously described mutation in the structural gene (pyrE) for orotate phosphoribosyltransferase (OPT) in Escherichia coli K-12. OPT from both the mutant...

Transformation of intact yeast cells treated with alkali cations.

Ito, H, Fukuda, Y, Murata, K, Kimura, A

Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae...

Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli.

Shimosaka, M, Fukuda, Y, Murata, K, Kimura, A

The closely linked structural genes of phosphofructokinase (pfkA) and triosephosphate isomerase (tpi) of Escherichia coli were separately cloned onto plasmid pBR322. By gene dosage effects,...

Regulation of ribonucleic acid synthesis in spheroplasts, cold-shocked cells, and toluene-treated cells of Escherichia coli.

Kimura, A

The effects on the stringent control of ribosomal ribonculeic acid synthesis of the removal of cell wall, cold-shock treatment of cells, LiCl treatment of toluene-treated cells, and hypotonic...

Phosphorylation of mononucleotides and formation of cytidine 5'-diphosphate-choline and sugar nucleotides by respiration-deficient mutants of yeasts.

Kimura, A, Hirose, K, Kariya, Y, Nagai, S

Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to...

Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains.

Murata, K, Fukuda, Y, Shimosaka, M, Watanabe, K, Saikusa, T, Kimura, A

The gene responsible for the methylglyoxal resistance of Saccharomyces cerevisiae was cloned, and its phenotypic characteristics were investigated. S. cerevisiae cells with the gene could accumulate...

Cloning of a gene responsible for the biosynthesis of glutathione in Escherichia coli B.

Murata, K, Kimura, A

A gene (gshI) responsible for gamma-glutamylcysteine synthetase (GSH-I) activity was cloned to construct an Escherichia coli B strain having high glutathione synthesizing activity. For this purpose,...

Group G streptococcal M protein exhibits structural features analogous to those of class I M protein of group A streptococci.

Collins, C M, Kimura, A, Bisno, A L

We have previously studied a collection of group G streptococcal strains isolated from bacteremic human infections and demonstrated that such strains resist phagocytosis by human polymorphonuclear...

A bactericidal monoclonal antibody specific for the lipooligosaccharide of Bordetella pertussis reduces colonization of the respiratory tract of mice after aerosol infection with B. pertussis.

Mountzouros, K T, Kimura, A, Cowell, J L

A mouse immunoglobulin G3 monoclonal antibody specific for the core oligosaccharide moiety of the lipooligosaccharide (LOS) of Bordetella pertussis has been shown to have complement-dependent...

Bordetella pertussis filamentous hemagglutinin: evaluation as a protective antigen and colonization factor in a mouse respiratory infection model.

Kimura, A, Mountzouros, K T, Relman, D A, Falkow, S, Cowell, J L

Filamentous hemagglutinin (FHA) is a cell surface protein of Bordetella pertussis which functions as an adhesin for this organism. It is a component of many new acellular pertussis vaccines. The...

Antigenic characterization of the oligosaccharide portion of the lipooligosaccharide of nontypable Haemophilus influenzae.

Patrick, C C, Kimura, A, Jackson, M A, Hermanstorfer, L, Hood, A, McCracken, G H, ...

Monoclonal antibodies (MAbs) directed against epitopes in the oligosaccharide portion of the lipooligosaccharide (LOS) of nontypable Haemophilus influenzae (NTHI) were used to characterize the LOS of...

Haemophilus influenzae type b lipooligosaccharide: stability of expression and association with virulence.

Kimura, A, Patrick, C C, Miller, E E, Cope, L D, McCracken, G H, Hansen, E J

Spontaneous antigenic and phenotypic variations in the lipooligosaccharide (LOS) of two strains of Haemophilus influenzae type b (Hib) were previously shown to be associated with changes in virulence...

Antigenic and phenotypic variations of Haemophilus influenzae type b lipopolysaccharide and their relationship to virulence.

Kimura, A, Hansen, E J

Haemophilus influenzae type b (Hib) strains NO100 and COL10 were found to produce bacteremia in infant rats at a much lower frequency than other Hib strains previously tested. These relatively...

A minor high-molecular-weight outer membrane protein of Haemophilus influenzae type b is a protective antigen.

Kimura, A, Gulig, P A, McCracken, G H, Loftus, T A, Hansen, E J

Cell surface-exposed antigenic determinants of several high-molecular-weight outer membrane proteins of Haemophilus influenzae type b (Hib) have been shown to be consistently immunogenic in human...

Increased capacity for glutathione synthesis enhances resistance to radiation in Escherichia coli: a possible model for mammalian cell protection.

Moore, W R, Anderson, M E, Meister, A, Murata, K, Kimura, A

A strain of Escherichia coli, enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase activities by recombinant DNA techniques, is more resistant to the lethal effects...

Gene conversion-like events cause steroid 21-hydroxylase deficiency in congenital adrenal hyperplasia.

Harada, F, Kimura, A, Iwanaga, T, Shimozawa, K, Yata, J, Sasazuki, T

Genomic DNAs from twelve Japanese patients with steroid 21-hydroxylase [21-OHase; steroid 21-monooxygenase; steroid, hydrogen-donor:oxygen oxidoreductase (21-hydroxylating); EC 1.14.99.10] deficiency...

A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.

Israël, A, Kimura, A, Kieran, M, Yano, O, Kanellopoulos, J, Le Bail, O, ...

Using gel retardation and in vitro "footprinting", we have analyzed the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of...

The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.

Watanabe, K, Yamano, Y, Murata, K, Kimura, A

The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted...

Nucleotide sequence of the gene responsible for D-xylose uptake in Escherichia coli.

Kurose, N, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature....

Pertussis toxin analog with reduced enzymatic and biological activities is a protective immunogen.

Kimura, A, Mountzouros, K T, Schad, P A, Cieplak, W, Cowell, J L

Bordetella pertussis TOX3201 has a 12-base-pair insertion in the S1 subunit gene of pertussis toxin (PTX), which encodes for a 4-amino-acid insertion between residues 107 and 108 of the mature S1...

Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.

Ohta, Y, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 1,243 bp in length coding for N-acetylneuraminate lyase (N-acetylneuraminate pyruvate lyase; NPL) of Escherichia coli has been determined. Nucleotide...

Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II.

Gushima, H, Yasuda, S, Soeda, E, Yokota, M, Kondo, M, Kimura, A

The nucleotide sequence of the cloned DNA, 1,478 bp in length coding for glutathione synthetase (GSH-II) of E. coli B has been determined. Amino acid and nucleotide sequence analyses have assigned...

Structure of cloned delta-globin genes from a normal subject and a patient with delta-thalassemia; sequence polymorphisms found in the delta-globin gene region of Japanese individuals.

Kimura, A, Matsunaga, E, Ohta, Y, Fujiyoshi, T, Matsuo, T, Nakamura, T, ...

The delta-globin genes of a normal Japanese and a Japanese patient with homozygous delta-thalassemia were cloned, and the nucleotide sequence of a region including the gene was determined. Comparison...

A frameshift addition causes silencing of the delta-globin gene in an old world monkey, an anubis (Papio doguera).

Kimura, A, Takagi, Y

A delta-globin gene of an anubis, Papio doguera, was cloned, and the nucleotide sequence of a region of about 2,000 bp including the gene was determined. In comparison with that of pSP3, a...

Importance of catalase in the adaptive response to hydrogen peroxide: analysis of acatalasaemic Saccharomyces cerevisiae.

Izawa, S, Inoue, Y, Kimura, A

Controversy about the importance of catalase in the detoxification of H2O2 in human erythrocytes continues. It has been suggested that catalase has no role in the clearance of H2O2 in erythrocytes....

Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae.

Izawa, S, Maeda, K, Miki, T, Mano, J, Inoue, Y, Kimura, A

Glucose-6-phosphate dehydrogenase (G6PDH)-deficient cells of Saccharomyces cerevisiae showed increased susceptibility and were unable to induce adaptation to oxidative stress. Historically, mainly in...

Cloning of the glucokinase gene in Escherichia coli B.

Fukuda, Y, Yamaguchi, S, Shimosaka, M, Murata, K, Kimura, A

A gene for glucokinase (Glk) in Escherichia coli B was cloned onto vector plasmid pBR322, and the hybrid plasmid obtained was designated pGK100. The gene for Glk was located in the central MluI...

Gene conversion in steroid 21-hydroxylase genes.

Urabe, K, Kimura, A, Harada, F, Iwanaga, T, Sasazuki, T

The steroid 21-hydroxylase gene, CYP21B, encodes cytochrome P450c21, which mediates 21-hydroxylation. The gene is located about 30 kb downstream from pseudogene CYP21A. The CYP21A gene is homologous...

Cloning and expression of a putative alcohol dehydrogenase gene of Entamoeba histolytica and its application to immunological examination.

Kimura, A, Hara, Y, Kimoto, T, Okuno, Y, Minekawa, Y, Nakabayashi, T

To clone and express the genes encoding major antigens of Entamoeba histolytica, we constructed a lambda gt11 cDNA library for E. histolytica HM1:IMSS and screened it with pooled sera from patients...

Modification of Escherichia coli B glutathione synthetase with polyethylene glycol for clinical application to enzyme replacement therapy for glutathione deficiency.

Inoue, Y, Sugiyama, K, Ueminami, H, Izawa, S, Kimura, A

Glutathione synthetase of Escherichia coli B was modified with polyethylene glycol, and the properties of the resultant modified enzyme were investigated. The thermal stability of the modified enzyme...

Purification and characterization of a novel nucleoside phosphorylase from a Klebsiella sp. and its use in the enzymatic production of adenine arabinoside.

Ling, F, Inoue, Y, Kimura, A

An adenosine-assimilating bacterium, Klebsiella sp. strain LF1202, inducibly formed a novel nucleoside phosphorylase which acted on both purine and pyrimidine nucleosides when the cells were cultured...

Role of human major histocompatibility complex DQ molecules in superantigenicity of streptococcus-derived protein.

Esaki, Y, Fukui, Y, Sudo, T, Yamamoto, K, Inamitsu, T, Nishimura, Y, ...

Antigenicity of peptic extract from type 12 group A streptococci (PEAST12) for T cells was examined in major histocompatibility complex (MHC) class II transgenic mice. PEAST12 was mitogenic for...

A microbial carbon-phosphorus bond cleavage enzyme requires two protein components for activity.

Murata, K, Higaki, N, Kimura, A

Enterobacter aerogenes IFO 12010 contains a carbon-phosphorus (C-P) bond cleavage enzyme catalyzing the liberation of inorganic phosphate from various alkyl- and phenylphosphonic acids. The enzyme in...

Purification and some properties of glutathione S-transferase from Escherichia coli B.

Iizuka, M, Inoue, Y, Murata, K, Kimura, A

Glutathione S-transferase was purified approximately 2,300-fold from cell extracts of Escherichia coli B with a 7.5% activity yield. The molecular weight of the enzyme was 45,000, and the enzyme...

Cystathionine accumulation in Saccharomyces cerevisiae.

Ono, B, Suruga, T, Yamamoto, M, Yamamoto, S, Murata, K, Kimura, A, ...

A cysteine-dependent strain of Saccharomyces cerevisiae and its prototrophic revertants accumulated cystathionine in cells. The cystathionine accumulation was caused by a single mutation having a...

Purine-mediated growth inhibition caused by a pyrE mutation in Escherichia coli K-12.

Shimosaka, M, Fukuda, Y, Murata, K, Kimura, A

A purine-sensitive phenotype results from a previously described mutation in the structural gene (pyrE) for orotate phosphoribosyltransferase (OPT) in Escherichia coli K-12. OPT from both the mutant...

Transformation of intact yeast cells treated with alkali cations.

Ito, H, Fukuda, Y, Murata, K, Kimura, A

Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae...

Cloning of the glucokinase gene in Escherichia coli B.

Fukuda, Y, Yamaguchi, S, Shimosaka, M, Murata, K, Kimura, A

A gene for glucokinase (Glk) in Escherichia coli B was cloned onto vector plasmid pBR322, and the hybrid plasmid obtained was designated pGK100. The gene for Glk was located in the central MluI...

Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli.

Shimosaka, M, Fukuda, Y, Murata, K, Kimura, A

The closely linked structural genes of phosphofructokinase (pfkA) and triosephosphate isomerase (tpi) of Escherichia coli were separately cloned onto plasmid pBR322. By gene dosage effects,...

Regulation of ribonucleic acid synthesis in spheroplasts, cold-shocked cells, and toluene-treated cells of Escherichia coli.

Kimura, A

The effects on the stringent control of ribosomal ribonculeic acid synthesis of the removal of cell wall, cold-shock treatment of cells, LiCl treatment of toluene-treated cells, and hypotonic...

Phosphorylation of mononucleotides and formation of cytidine 5'-diphosphate-choline and sugar nucleotides by respiration-deficient mutants of yeasts.

Kimura, A, Hirose, K, Kariya, Y, Nagai, S

Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to...

Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains.

Murata, K, Fukuda, Y, Shimosaka, M, Watanabe, K, Saikusa, T, Kimura, A

The gene responsible for the methylglyoxal resistance of Saccharomyces cerevisiae was cloned, and its phenotypic characteristics were investigated. S. cerevisiae cells with the gene could accumulate...

Cloning of a gene responsible for the biosynthesis of glutathione in Escherichia coli B.

Murata, K, Kimura, A

A gene (gshI) responsible for gamma-glutamylcysteine synthetase (GSH-I) activity was cloned to construct an Escherichia coli B strain having high glutathione synthesizing activity. For this purpose,...

Group G streptococcal M protein exhibits structural features analogous to those of class I M protein of group A streptococci.

Collins, C M, Kimura, A, Bisno, A L

We have previously studied a collection of group G streptococcal strains isolated from bacteremic human infections and demonstrated that such strains resist phagocytosis by human polymorphonuclear...

A bactericidal monoclonal antibody specific for the lipooligosaccharide of Bordetella pertussis reduces colonization of the respiratory tract of mice after aerosol infection with B. pertussis.

Mountzouros, K T, Kimura, A, Cowell, J L

A mouse immunoglobulin G3 monoclonal antibody specific for the core oligosaccharide moiety of the lipooligosaccharide (LOS) of Bordetella pertussis has been shown to have complement-dependent...

Bordetella pertussis filamentous hemagglutinin: evaluation as a protective antigen and colonization factor in a mouse respiratory infection model.

Kimura, A, Mountzouros, K T, Relman, D A, Falkow, S, Cowell, J L

Filamentous hemagglutinin (FHA) is a cell surface protein of Bordetella pertussis which functions as an adhesin for this organism. It is a component of many new acellular pertussis vaccines. The...

Antigenic characterization of the oligosaccharide portion of the lipooligosaccharide of nontypable Haemophilus influenzae.

Patrick, C C, Kimura, A, Jackson, M A, Hermanstorfer, L, Hood, A, McCracken, G H, ...

Monoclonal antibodies (MAbs) directed against epitopes in the oligosaccharide portion of the lipooligosaccharide (LOS) of nontypable Haemophilus influenzae (NTHI) were used to characterize the LOS of...

Haemophilus influenzae type b lipooligosaccharide: stability of expression and association with virulence.

Kimura, A, Patrick, C C, Miller, E E, Cope, L D, McCracken, G H, Hansen, E J

Spontaneous antigenic and phenotypic variations in the lipooligosaccharide (LOS) of two strains of Haemophilus influenzae type b (Hib) were previously shown to be associated with changes in virulence...

Antigenic and phenotypic variations of Haemophilus influenzae type b lipopolysaccharide and their relationship to virulence.

Kimura, A, Hansen, E J

Haemophilus influenzae type b (Hib) strains NO100 and COL10 were found to produce bacteremia in infant rats at a much lower frequency than other Hib strains previously tested. These relatively...

A minor high-molecular-weight outer membrane protein of Haemophilus influenzae type b is a protective antigen.

Kimura, A, Gulig, P A, McCracken, G H, Loftus, T A, Hansen, E J

Cell surface-exposed antigenic determinants of several high-molecular-weight outer membrane proteins of Haemophilus influenzae type b (Hib) have been shown to be consistently immunogenic in human...

Increased capacity for glutathione synthesis enhances resistance to radiation in Escherichia coli: a possible model for mammalian cell protection.

Moore, W R, Anderson, M E, Meister, A, Murata, K, Kimura, A

A strain of Escherichia coli, enriched in its content of gamma-glutamylcysteine synthetase and glutathione synthetase activities by recombinant DNA techniques, is more resistant to the lethal effects...

Gene conversion-like events cause steroid 21-hydroxylase deficiency in congenital adrenal hyperplasia.

Harada, F, Kimura, A, Iwanaga, T, Shimozawa, K, Yata, J, Sasazuki, T

Genomic DNAs from twelve Japanese patients with steroid 21-hydroxylase [21-OHase; steroid 21-monooxygenase; steroid, hydrogen-donor:oxygen oxidoreductase (21-hydroxylating); EC 1.14.99.10] deficiency...

A common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.

Israël, A, Kimura, A, Kieran, M, Yano, O, Kanellopoulos, J, Le Bail, O, ...

Using gel retardation and in vitro "footprinting", we have analyzed the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of...

The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.

Watanabe, K, Yamano, Y, Murata, K, Kimura, A

The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted...

Nucleotide sequence of the gene responsible for D-xylose uptake in Escherichia coli.

Kurose, N, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature....

Pertussis toxin analog with reduced enzymatic and biological activities is a protective immunogen.

Kimura, A, Mountzouros, K T, Schad, P A, Cieplak, W, Cowell, J L

Bordetella pertussis TOX3201 has a 12-base-pair insertion in the S1 subunit gene of pertussis toxin (PTX), which encodes for a 4-amino-acid insertion between residues 107 and 108 of the mature S1...

Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.

Ohta, Y, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 1,243 bp in length coding for N-acetylneuraminate lyase (N-acetylneuraminate pyruvate lyase; NPL) of Escherichia coli has been determined. Nucleotide...

Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II.

Gushima, H, Yasuda, S, Soeda, E, Yokota, M, Kondo, M, Kimura, A

The nucleotide sequence of the cloned DNA, 1,478 bp in length coding for glutathione synthetase (GSH-II) of E. coli B has been determined. Amino acid and nucleotide sequence analyses have assigned...

Structure of cloned delta-globin genes from a normal subject and a patient with delta-thalassemia; sequence polymorphisms found in the delta-globin gene region of Japanese individuals.

Kimura, A, Matsunaga, E, Ohta, Y, Fujiyoshi, T, Matsuo, T, Nakamura, T, ...

The delta-globin genes of a normal Japanese and a Japanese patient with homozygous delta-thalassemia were cloned, and the nucleotide sequence of a region including the gene was determined. Comparison...

A frameshift addition causes silencing of the delta-globin gene in an old world monkey, an anubis (Papio doguera).

Kimura, A, Takagi, Y

A delta-globin gene of an anubis, Papio doguera, was cloned, and the nucleotide sequence of a region of about 2,000 bp including the gene was determined. In comparison with that of pSP3, a...

Importance of catalase in the adaptive response to hydrogen peroxide: analysis of acatalasaemic Saccharomyces cerevisiae.

Izawa, S, Inoue, Y, Kimura, A

Controversy about the importance of catalase in the detoxification of H2O2 in human erythrocytes continues. It has been suggested that catalase has no role in the clearance of H2O2 in erythrocytes....

Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae.

Izawa, S, Maeda, K, Miki, T, Mano, J, Inoue, Y, Kimura, A

Glucose-6-phosphate dehydrogenase (G6PDH)-deficient cells of Saccharomyces cerevisiae showed increased susceptibility and were unable to induce adaptation to oxidative stress. Historically, mainly in...

Gene conversion in steroid 21-hydroxylase genes.

Urabe, K, Kimura, A, Harada, F, Iwanaga, T, Sasazuki, T

The steroid 21-hydroxylase gene, CYP21B, encodes cytochrome P450c21, which mediates 21-hydroxylation. The gene is located about 30 kb downstream from pseudogene CYP21A. The CYP21A gene is homologous...

Acute myocardial infarction caused by thrombotic occlusion at a stent site two years after conventional stent implantation

Hayashi, T, Kimura, A, Ishikawa, K

Two cases of acute myocardial infarction caused by thrombotic occlusion at the conventional stented site two years after stenting are described. Late thrombotic stent occlusion may be caused by...

Development of non-cholinergic, non-adrenergic excitatory and inhibitory responses to intramural nerve stimulation in rat stomach.

Ito, S., Kimura, A., Ohga, A.

1. The onset and development of functional innervation of intramural neurones were examined by transmural nerve stimulation in circular muscle strips isolated from the rat stomach during the period...

Developmental pattern of 3-oxo-Δ4 bile acids in neonatal bile acid metabolism

Inoue, T., Kimura, A., Aoki, K., Tohma, M., Kato, H.

AIMS—To investigate whether a fetal pathway of bile acid synthesis persists in neonates and infants.
METHODS—3-oxo-Δ4 bile acids were determined qualitatively and quantitatively in the urine,...

Analysis of histological therapeutic effect, apoptosis rate and p53 status after combined treatment with radiation, hyperthermia and 5-fluorouracil suppositories for advanced rectal cancers.

Sakakura, C., Koide, K., Ichikawa, D., Wakasa, T., Shirasu, M., Kimura, A., ...

The tumour-suppressor gene p53 encodes a transcription factor that plays a critical role in the induction of G1 cell cycle arrest and apoptosis after DNA damage. To clarify the role of the p53 gene...

Discrimination of double primary lung cancer from intrapulmonary metastasis by p53 gene mutation

Matsuzoe, D, Hideshima, T, Ohshima, K, Kawahara, K, Shirakusa, T, Kimura, A

When multiple synchronous lung tumours are identified, discrimination of multicentric lung cancers from intrapulmonary metastases by clinical findings is often difficult. We used genetic alterations...