Scientific Commons: Arthur A. Guffanti

mrp, a Multigene, Multifunctional Locus in Bacillus subtilis with Roles in Resistance to Cholate and to Na+ and in pH Homeostasis

Ito, Masahiro, Guffanti, Arthur A., Oudega, Bauke, Krulwich, Terry A.

A 5.9-kb region of the Bacillus subtilis chromosome is transcribed as a single transcript that is predicted to encode seven membrane-spanning proteins. Homologues of the first gene of this operon,...

Effects of Nonpolar Mutations in Each of the Seven Bacillus subtilis mrp Genes Suggest Complex Interactions among the Gene Products in Support of Na+ and Alkali but Not Cholate Resistance

Ito, Masahiro, Guffanti, Arthur A., Wang, Wei, Krulwich, Terry A.

The Bacillus subtilis mrp (multiple resistance and pH) operon supports Na+ and alkali resistance via an Na+/H+ antiport, as well as cholate efflux and resistance. Among the individual mutants with...

Two-Dimensional Gel Electrophoresis Analyses of pH-Dependent Protein Expression in Facultatively Alkaliphilic Bacillus pseudofirmus OF4 Lead to Characterization of an S-Layer Protein with a Role in Alkaliphily

Gilmour, Raymond, Messner, Paul, Guffanti, Arthur A., Kent, Rebecca, Scheberl, Andrea, Kendrick, Nancy, ...

The large majority of proteins of alkaliphilic Bacillus pseudofirmus OF4 grown at pH 7.5 and 10.5, as studied by two-dimensional gel electrophoresis analyses, did not exhibit significant pH-dependent...

Twelve-Transmembrane-Segment (TMS) Version (ΔTMS VII-VIII) of the 14-TMS Tet(L) Antibiotic Resistance Protein Retains Monovalent Cation Transport Modes but Lacks Tetracycline Efflux Capacity

Jin, Jie, Guffanti, Arthur A., Beck, Catherine, Krulwich, Terry A.

A “Tet(L)-12” version of Tet(L), a tetracycline efflux protein with 14 transmembrane segments (TMS), was constructed by deletion of two central TMS. Tet(L)-12 catalyzed Na+/H+ antiport and...

GerN, an Endospore Germination Protein of Bacillus cereus, Is an Na+/H+-K+ Antiporter

Southworth, Thomas W., Guffanti, Arthur A., Moir, Anne, Krulwich, Terry A.

GerN, a Bacillus cereus spore germination protein, exhibits homology to a widely distributed group of putative cation transporters or channel proteins. GerN complemented the Na+-sensitive phenotype...

Two Types of Bacillus subtilis tetA(L) Deletion Strains Reveal the Physiological Importance of TetA(L) in K+ Acquisition as well as in Na+, Alkali, and Tetracycline Resistance

Wang, Wei, Guffanti, Arthur A., Wei, Yi, Ito, Masahiro, Krulwich, Terry A.

The chromosomally encoded TetA(L) protein of Bacillus subtilis is a multifunctional tetracycline-metal/H+ antiporter that also exhibits monovalent cation/H+ antiport activity and a net K+ uptake...

Tet(L) and Tet(K) Tetracycline-Divalent Metal/H+ Antiporters: Characterization of Multiple Catalytic Modes and a Mutagenesis Approach to Differences in Their Efflux Substrate and Coupling Ion Preferences

Jin, Jie, Guffanti, Arthur A., Bechhofer, David H., Krulwich, Terry A.

The Tet(L) protein encoded in the Bacillus subtilis chromosome and the closely related Tet(K) protein from Staphylococcus aureus plasmids are multifunctional antiporters that have three cytoplasmic...

Mutational Loss of a K+ and NH4+ Transporter Affects the Growth and Endospore Formation of Alkaliphilic Bacillus pseudofirmus OF4

Wei, Yi, Southworth, Thomas W., Kloster, Hilde, Ito, Masahiro, Guffanti, Arthur A., Moir, Anne, ...

A putative transport protein (Orf9) of alkaliphilic Bacillus pseudofirmus OF4 belongs to a transporter family (CPA-2) of diverse K+ efflux proteins and cation antiporters. Orf9 greatly increased the...

A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake

Hicks, David B., Wang, ZhenXiong, Wei, Yi, Kent, Rebecca, Guffanti, Arthur A., Banciu, Horia, ...

The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes...

Conversion of Pyruvate to Acetoin Helps To Maintain pH Homeostasis in Lactobacillus plantarum†

Tsau, Jya-Li, Guffanti, Arthur A., Montville, Thomas J.

Pyruvate is the substrate for diacetyl and acetoin synthesis by lactobacilli. Exogenous pyruvate stimulates acetoin production when glucose is present as an energy source. In Lactobacillus plantarum...

The voltage-gated Na+ channel NaVBP has a role in motility, chemotaxis, and pH homeostasis of an alkaliphilic Bacillus

Ito, Masahiro, Xu, Haoxing, Guffanti, Arthur A., Wei, Yi, Zvi, Lior, Clapham, David E., ...

The prokaryotic voltage-gated Na+ channel, NaChBac, is one of a growing channel superfamily of unknown function. Here we show that NaVBP, the NaChBac homologue encoded by ncbA in alkaliphilic...

The Mrp Na+/H+ Antiporter Increases the Activity of the Malate:Quinone Oxidoreductase of an Escherichia coli Respiratory Mutant

Swartz, Talia H., Ito, Masahiro, Hicks, David B., Nuqui, Mark, Guffanti, Arthur A., Krulwich, Terry A.

Mrp catalyzes secondary Na+/H+ antiport and was hypothesized to have an additional primary energization mode. Mrp-dependent complementation of nonfermentative growth of an Escherichia coli...