Desai, Nisarg R, Kesari, Kavindra K, Agarwal, Ashok
Abstract Hazardous health effects stemming from exposure to radiofrequency electromagnetic waves (RF-EMW) emitted from cell phones have been reported in the literature. However, the cellular target...
Factors affecting the outcome of human blastocyst vitrification (2009)
Kader, Amr A, Choi, Audrey, Orief, Yasser, Agarwal, Ashok
Abstract With single blastocyst transfer practice becoming more common in ART, there is a greater demand for a convenient and reliable cryostorage of surplus blastocysts. Vitrification has emerged in...
Vitrification of isolated mice blastomeres using a closed loading device (2009)
Kader, Amr, Agarwal, Ashok, Sharma, Rakesh, Falcone, Tommaso
Abstract Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic...
Factors affecting the outcome of human blastocyst vitrification. (2009)
Kader, Amr A, Choi, Audrey, Orief, Yasser, Agarwal, Ashok
With single blastocyst transfer practice becoming more common in ART, there is a greater demand for a convenient and reliable cryostorage of surplus blastocysts. Vitrification has emerged in the last...
EDITOR EDITOR EDITOR EDITOR EDITOR (2008)
K. L. Jaisingh, Ashok Agarwal, D. C. Bajaj, V. V. Deodhar, Vijay Jhalani, ...
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Varghese, Alex C, Du Plessis, Stefan S, Falcone, Tommaso, Agarwal, Ashok
Abstract Cryopreservation of ovarian tissue and in vitro follicle maturation are two emerging techniques for fertility preservation, especially in cancer patients. These treatment regimes are opening...
Cocuzza,Marcello, Sikka,Suresh C., Athayde,Kelly S., Agarwal,Ashok
Oxidative stress (OS) in the reproductive tract is now a real entity and concern due to the potential harmful effects of high levels of reactive oxygen species (ROS) on sperm number, motility,...
Oxidative stress and antioxidants in male infertility: a difficult balance (2007)
Agarwal, Ashok, Prabakaran, Sushil Anandh
Infertility is one of the most stressful conditions amongst married couples. Male factor infertility is implicated in almost half of these cases. Recent advances in the field of reproductive medicine...
Esteves,Sandro C., Sharma,Rakesh K., Thomas Jr,Anthony J., Agarwal,Ashok
OBJECTIVE: In this study, we evaluated whether the hypo-osmotic swelling test (HOST) can be used as a vital marker in combination with peanut agglutinin (PNA) - labeling in fresh and cryopreserved...
The relationship between human sperm apoptosis, morphology and the sperm deformity index (2007)
Aziz, Nabil, Said, Tamer, Paasch, Uwe, Agarwal, Ashok
BACKGROUND This study aimed to assess the relationship between apoptosis in human ejaculated spermatozoa, sperm morphology and the novel sperm deformity index (SDI). METHODS Semen specimens from 50...
The relationship between human sperm apoptosis, morphology and the sperm deformity index (2007)
Aziz, Nabil, Said, Tamer, Paasch, Uwe, Agarwal, Ashok
BACKGROUND This study aimed to assess the relationship between apoptosis in human ejaculated spermatozoa, sperm morphology and the novel sperm deformity index (SDI). METHODS Semen specimens from 50...
The relationship between human sperm apoptosis, morphology and the sperm deformity index (2007)
Aziz, Nabil, Said, Tamer, Paasch, Uwe, Agarwal, Ashok
BACKGROUND This study aimed to assess the relationship between apoptosis in human ejaculated spermatozoa, sperm morphology and the novel sperm deformity index (SDI). METHODS Semen specimens from 50...
Tamer Said, Ashok Agarwal, Sonja Grunewald, Manja Rasch, Thomas Baumann, Christian Kriegel, ...
Magnetic cell sorting (MACS) using annexin V-conjugated microbeads eliminates apoptotic spermatozoa based on the externalization of phosphatidylserine residues. The procedure delivers two sperm...
Bedaiwy, Mohamed A., Falcone, Tommaso, Goldberg, Jeffrey M., Sharma, Rakesh K., Nelson, David R., Agarwal, Ashok
BACKGROUND: Leptin influences the proinflammatory immune responses and has angiogenic activity in vitro and in vivo. The objective of this study was to evaluate the peritoneal fluid levels of leptin...
Nallella,Kiran P., Sharma,Rakesh K., Allamaneni,Shyam S. R., Agarwal,Ashok
PURPOSE: To determine whether patients with male factor infertility can be accurately identified by calculating a novel semen quality score and measuring levels of reactive oxygen species during...
Disruption of Spermatogenesis by the Cancer Disease Process (2005)
Agarwal, Ashok, Allamaneni, Shyam S. R.
In the past, cancer survivors tended to be most concerned about disease recurrence and treatment side effects. As survival rates have increased, however, patients are now also concerned about...
Bedaiwy, Mohamed A., Falcone, Tommaso, Goldberg, Jeffrey M., Sharma, Rakesh K., Nelson, David R., Agarwal, Ashok
BACKGROUND: Leptin influences the proinflammatory immune responses and has angiogenic activity in vitro and in vivo. The objective of this study was to evaluate the peritoneal fluid levels of leptin...
Uwe Paasch, Rakesh K. Sharma, Akshay K. Gupta, Sonja Grunewald, Edward J. Mascha, ...
We investigated the impact of cryopreservation and thawing on levels of caspases-3, -8, and -9 activity, intact mitochondrial membrane potential (Δψm), and DNA fragmentation in human spermatozoa....
Role of caspases in male infertility (2004)
Said, Tamer M., Paasch, Uwe, Glander, Hans-Juergen, Agarwal, Ashok
Apoptosis is characterized by a variety of changes resulting in the recognition and phagocytosis of apoptotic cells. Caspases (cysteinyl aspartate‐specific proteinases) play a central role in...
Moustafa, Mohamed H., Sharma, Rakesh K., Thornton, Julie, Mascha, Edward, Abdel-Hafez, Mohammed A., Thomas, Anthony J., ...
BACKGROUND: The aim of this study was to examine the role of apoptosis and reactive oxygen species (ROS) in inducing DNA damage in ejaculated spermatozoa. METHODS: We examined ejaculated spermatozoa...
Detection of testicular cancer in men presenting with infertility (2003)
Pasqualotto,Fabio Firmbach, Pasqualotto,Eleonora Bedin, Agarwal,Ashok, Thomas Jr.,Anthony Joseph
PURPOSE: Infertility is one of the less common presenting features associated with testicular tumors. We evaluated the histologic and biochemical findings, and pregnancy outcome in patients...
Pasqualotto,Fabio Firmbach, Pasqualotto,Eleonora Bedin, Agarwal,Ashok, Thomas Jr.,Anthony Joseph
The incidence of vasal injury during inguinal herniorrhaphy is estimated at 0.5%. We sought to assess the patency rates and long-term fertility outcome after microsurgical repair of vasal obstruction...
Lower sperm aneuploidy frequency is associated with high pregnancy rates in ICSI programmes (2003)
Burrello, Nunziatina, Vicari, Enzo, Shin, Paul, Agarwal, Ashok, De Palma, Adele, Grazioso, Caterina, ...
BACKGROUND: A large proportion of patients undergoing ICSI have been shown to have an increased sperm aneuploidy rate. This study was undertaken to evaluate the impact of sperm aneuploidy on ICSI...
Varicocele and male infertility: Part II: Pathophysiology of varicoceles in male infertility (2001)
Naughton, Cathy K., Nangia, Ajay K., Agarwal, Ashok
Varicoceles are found in 19 to 41% of infertile men, and is one treatable form of male infertility. The mechanism by which varicoceles cause the variable effect on male infertility and...
Sharma, Rakesh K., Pasqualotto, Fabio F., Nelson, David R., Thomas, Anthony J., Agarwal, Ashok
The imbalance between reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in seminal fluid indicates oxidative stress and is correlated with male infertility. A composite...
Role of oxidative stress in female reproduction
Agarwal, Ashok, Gupta, Sajal, Sharma, Rakesh K
In a healthy body, ROS (reactive oxygen species) and antioxidants remain in balance. When the balance is disrupted towards an overabundance of ROS, oxidative stress (OS) occurs. OS influences the...
Varghese, Alex C, Du Plessis, Stefan S, Falcone, Tommaso, Agarwal, Ashok
Cryopreservation of ovarian tissue and in vitro follicle maturation are two emerging techniques for fertility preservation, especially in cancer patients. These treatment regimes are opening up more...
Vitrification of isolated mice blastomeres using a closed loading device
Kader, Amr, Agarwal, Ashok, Sharma, Rakesh, Falcone, Tommaso
Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic information. A...
The Role of Varicocele Repair in the New Era of Assisted Reproductive Technology
Cocuzza, Marcello, Cocuzza, Mariana Amora, Bragais, Frances Monette Papa, Agarwal, Ashok
Infertility affects 10–15% of couples who are trying to conceive, and half of the cases are due to male infertility. Intracytoplasmic sperm injection is increasingly being used to overcome multiple...
Nonsurgical treatment of male infertility: specific and empiric therapy
Cocuzza, Marcello, Agarwal, Ashok
Management of male infertility is always a difficult task, and the pathologic process is often poorly understood. Even though modern assisted reproduction techniques (ART) can help overcome severe...
Factors affecting the outcome of human blastocyst vitrification
Kader, Amr A, Choi, Audrey, Orief, Yasser, Agarwal, Ashok
With single blastocyst transfer practice becoming more common in ART, there is a greater demand for a convenient and reliable cryostorage of surplus blastocysts. Vitrification has emerged in the last...
Desai, Nisarg R, Kesari, Kavindra K, Agarwal, Ashok
Hazardous health effects stemming from exposure to radiofrequency electromagnetic waves (RF-EMW) emitted from cell phones have been reported in the literature. However, the cellular target of RF-EMW...