Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development.
Dykhuizen, D E, Polin, D S, Dunn, J J, Wilske, B, Preac-Mursic, V, Dattwyler, R J, ...
The chromosomal genes fla and p93 and the ospA gene from a linear plasmid were sequenced from up to 15 isolates of Borrelia burgdorferi, which causes Lyme borreliosis in man. Comparison of the gene...
Kumaran, D., Eswaramoorthy, S., Luft, B.J., Koide, S., Dunn, J.J., Lawson, C.L., ...
Outer surface protein C (OspC) is a major antigen on the surface of the Lyme disease spirochete, Borrelia burgdorferi, when it is being transmitted to humans. Crystal structures of OspC have been...
Clarithromycin in treatment of early Lyme disease: a pilot study.
Dattwyler, R J, Grunwaldt, E, Luft, B J
Forty-one patients with erythema migrans were enrolled in an open-labelled pilot study of oral clarithromycin, 500 mg twice daily for 21 days, for the treatment of early Lyme disease. Immediately...
Jacobson, J M, Davidian, M, Rainey, P M, Hafner, R, Raasch, R H, Luft, B J
Pyrimethamine pharmacokinetics were studied in 11 human immunodeficiency virus (HIV)-positive patients who were seropositive for exposure to Toxoplasma gondii and were taking zidovudine (AIDS...
Silverman, J A, Hayes, M L, Luft, B J, Joiner, K A
The immunosuppressive agent cyclosporin A (CsA) also possesses broad-spectrum antimicrobial activity. Previous investigators have reported that the obligate intracellular protozoan Toxoplasma gondii...
McGrath, B C, Dunn, J J, Gorgone, G, Guttman, D, Dykhuizen, D, Luft, B J
The gene for the major outer surface protein A (OspA) from several clinically obtained strains of Borrelia burgdorferi, the cause of Lyme disease, has been cloned, sequenced, and expressed in...
Piesman, J, Dolan, M C, Happ, C M, Luft, B J, Rooney, S E, Mather, T N, ...
The ability of naturally infected and cured mice to resist reinfection with tick-transmitted Borrelia burgdorferi was tested over a 1-year period. All of the mice were resistant to reinfection when...
Activity of roxithromycin (RU 28965), a macrolide, against Toxoplasma gondii infection in mice.
Roxithromycin (RU 28965), an ether oxime derivative of erythromycin, protected mice against a lethal infection with the virulent RH strain of Toxoplasma gondii. Therapy begun 24 h before, 2 h after,...
Dunn, J J, Buchstein, S R, Butler, L L, Fisenne, S, Polin, D S, Lade, B N, ...
We have determined the complete nucleotide sequence of a small circular plasmid from the spirochete Borrelia burgdorferi Ip21, the agent of Lyme disease. The plasmid (cp8.3/Ip21) is 8,303 bp long,...
Guttman, D S, Wang, P W, Wang, I N, Bosler, E M, Luft, B J, Dykhuizen, D E
The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism...
Borrelia burgdorferi HSP70 homolog: characterization of an immunoreactive stress protein.
Anzola, J, Luft, B J, Gorgone, G, Dattwyler, R J, Soderberg, C, Lahesmaa, R, ...
The gene encoding an immunoreactive Borrelia burgdorferi HSP70 homolog was isolated and characterized. The predicted amino acid sequence of this spirochetal protein confirms that this gene encodes a...
The 93-kilodalton protein of Borrelia burgdorferi: an immunodominant protoplasmic cylinder antigen.
Luft, B J, Mudri, S, Jiang, W, Dattwyler, R J, Gorevic, P D, Fischer, T, ...
Using immunoblots, we identified proteins of Borrelia burgdorferi recognized by sera from 62 patients with either acute or chronic Lyme disease. In all groups studied, the 41-kDa flagellar protein...
Schubach, W H, Mudri, S, Dattwyler, R J, Luft, B J
The major outer surface membrane protein of Borrelia burgdorferi, OspA, is one of several antigens recognized by sera from some patients in the chronic phase of Lyme borreliosis. We have expressed...
Characterization of a Borrelia burgdorferi dnaJ homolog.
Anzola, J, Luft, B J, Gorgone, G, Peltz, G
The gene encoding a Borrelia burgdorferi DnaJ homolog, located immediately 3' of the hsp70 gene, was characterized. Although there is a single copy of the dnaJ gene on the spirochetal chromosome, two...
Biochemical and immunological characterization of the surface proteins of Borrelia burgdorferi.
Luft, B J, Jiang, W, Munoz, P, Dattwyler, R J, Gorevic, P D
The immunodominant proteins and glycoproteins of Borrelia burgdorferi were analyzed by one-dimensional (1D) and 2D gel electrophoresis. More than 100 polypeptide species could be detected on...
Characterization of the Cryptosporidium antigens from sporulated oocysts of Cryptosporidium parvum.
Luft, B J, Payne, D, Woodmansee, D, Kim, C W
The antigenic constituents of sporulated Cryptosporidium parvum oocyst antigens were characterized with antisera from mice immunized against C. parvum. Sodium dodecyl sulfate-polyacrylamide gel...
Mapping the major antigenic domains of the native flagellar antigen of Borrelia burgdorferi.
Jiang, W, Luft, B J, Schubach, W, Dattwyler, R J, Gorevic, P D
Purified flagellar protein (p41) of Borrelia burgdorferi (strain B31) was subjected to chemical cleavage with hydroxylamine or proteolysis with V8 protease, endoproteinase Asp-N, or...
Beckers, C J, Roos, D S, Donald, R G, Luft, B J, Schwab, J C, Cao, Y, ...
We investigated potential targets for the activity of protein synthesis inhibitors against the protozoan parasite Toxoplasma gondii. Although nanomolar concentrations of azithromycin and clindamycin...
Early and specific antibody response to OspA in Lyme Disease.
Schutzer, S E, Coyle, P K, Dunn, J J, Luft, B J, Brunner, M
Borrelia burgdorferi (Bb), the cause of Lyme disease, has appeared not to evoke a detectable specific antibody response in humans until long after infection. This delayed response has been a biologic...
Studies of the effect of pregnancy on the capacity of mice to resist Listeria monocytogenes and Toxoplasma gondii infection revealed significantly diminished resistance of pregnant mice to infection...
Jiang, W, Gorevic, P D, Dattwyler, R J, Dunn, J J, Luft, B J
The major outer surface protein, OspA, of Borrelia burgdorferi is a lipoprotein which is a particular interest because of its potential as a vaccine candidate. However, serotypic and genetic analysis...
Schutzer, S E, Coyle, P K, Krupp, L B, Deng, Z, Belman, A L, Dattwyler, R, ...
Lyme disease is the major tick-borne disease, caused by Borrelia burgdorferi (Bb). Neurological involvement is common in all stages. In vivo expression of Bb antigens (Ags) and the immune response to...
Penicillin-binding proteins in Borrelia burgdorferi.
Urban, C, Rahal, J J, Dattwyller, R J, Gorevic, P, Luft, B J
Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band...
Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto.
Wang, I N, Dykhuizen, D E, Qiu, W, Dunn, J J, Bosler, E M, Luft, B J
The outer surface protein, OspC, is highly variable in Borrelia burgdorferi sensu stricto, the agent of Lyme disease. We have shown that even within a single population OspC is highly variable. The...
Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development.
Dykhuizen, D E, Polin, D S, Dunn, J J, Wilske, B, Preac-Mursic, V, Dattwyler, R J, ...
The chromosomal genes fla and p93 and the ospA gene from a linear plasmid were sequenced from up to 15 isolates of Borrelia burgdorferi, which causes Lyme borreliosis in man. Comparison of the gene...
Kumaran, D., Eswaramoorthy, S., Luft, B.J., Koide, S., Dunn, J.J., Lawson, C.L., ...
Outer surface protein C (OspC) is a major antigen on the surface of the Lyme disease spirochete, Borrelia burgdorferi, when it is being transmitted to humans. Crystal structures of OspC have been...
Clarithromycin in treatment of early Lyme disease: a pilot study.
Dattwyler, R J, Grunwaldt, E, Luft, B J
Forty-one patients with erythema migrans were enrolled in an open-labelled pilot study of oral clarithromycin, 500 mg twice daily for 21 days, for the treatment of early Lyme disease. Immediately...
Jacobson, J M, Davidian, M, Rainey, P M, Hafner, R, Raasch, R H, Luft, B J
Pyrimethamine pharmacokinetics were studied in 11 human immunodeficiency virus (HIV)-positive patients who were seropositive for exposure to Toxoplasma gondii and were taking zidovudine (AIDS...
Silverman, J A, Hayes, M L, Luft, B J, Joiner, K A
The immunosuppressive agent cyclosporin A (CsA) also possesses broad-spectrum antimicrobial activity. Previous investigators have reported that the obligate intracellular protozoan Toxoplasma gondii...
McGrath, B C, Dunn, J J, Gorgone, G, Guttman, D, Dykhuizen, D, Luft, B J
The gene for the major outer surface protein A (OspA) from several clinically obtained strains of Borrelia burgdorferi, the cause of Lyme disease, has been cloned, sequenced, and expressed in...
Piesman, J, Dolan, M C, Happ, C M, Luft, B J, Rooney, S E, Mather, T N, ...
The ability of naturally infected and cured mice to resist reinfection with tick-transmitted Borrelia burgdorferi was tested over a 1-year period. All of the mice were resistant to reinfection when...
Activity of roxithromycin (RU 28965), a macrolide, against Toxoplasma gondii infection in mice.
Roxithromycin (RU 28965), an ether oxime derivative of erythromycin, protected mice against a lethal infection with the virulent RH strain of Toxoplasma gondii. Therapy begun 24 h before, 2 h after,...
Dunn, J J, Buchstein, S R, Butler, L L, Fisenne, S, Polin, D S, Lade, B N, ...
We have determined the complete nucleotide sequence of a small circular plasmid from the spirochete Borrelia burgdorferi Ip21, the agent of Lyme disease. The plasmid (cp8.3/Ip21) is 8,303 bp long,...
Guttman, D S, Wang, P W, Wang, I N, Bosler, E M, Luft, B J, Dykhuizen, D E
The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism...
Borrelia burgdorferi HSP70 homolog: characterization of an immunoreactive stress protein.
Anzola, J, Luft, B J, Gorgone, G, Dattwyler, R J, Soderberg, C, Lahesmaa, R, ...
The gene encoding an immunoreactive Borrelia burgdorferi HSP70 homolog was isolated and characterized. The predicted amino acid sequence of this spirochetal protein confirms that this gene encodes a...
The 93-kilodalton protein of Borrelia burgdorferi: an immunodominant protoplasmic cylinder antigen.
Luft, B J, Mudri, S, Jiang, W, Dattwyler, R J, Gorevic, P D, Fischer, T, ...
Using immunoblots, we identified proteins of Borrelia burgdorferi recognized by sera from 62 patients with either acute or chronic Lyme disease. In all groups studied, the 41-kDa flagellar protein...
Schubach, W H, Mudri, S, Dattwyler, R J, Luft, B J
The major outer surface membrane protein of Borrelia burgdorferi, OspA, is one of several antigens recognized by sera from some patients in the chronic phase of Lyme borreliosis. We have expressed...
Characterization of a Borrelia burgdorferi dnaJ homolog.
Anzola, J, Luft, B J, Gorgone, G, Peltz, G
The gene encoding a Borrelia burgdorferi DnaJ homolog, located immediately 3' of the hsp70 gene, was characterized. Although there is a single copy of the dnaJ gene on the spirochetal chromosome, two...
Biochemical and immunological characterization of the surface proteins of Borrelia burgdorferi.
Luft, B J, Jiang, W, Munoz, P, Dattwyler, R J, Gorevic, P D
The immunodominant proteins and glycoproteins of Borrelia burgdorferi were analyzed by one-dimensional (1D) and 2D gel electrophoresis. More than 100 polypeptide species could be detected on...
Characterization of the Cryptosporidium antigens from sporulated oocysts of Cryptosporidium parvum.
Luft, B J, Payne, D, Woodmansee, D, Kim, C W
The antigenic constituents of sporulated Cryptosporidium parvum oocyst antigens were characterized with antisera from mice immunized against C. parvum. Sodium dodecyl sulfate-polyacrylamide gel...
Mapping the major antigenic domains of the native flagellar antigen of Borrelia burgdorferi.
Jiang, W, Luft, B J, Schubach, W, Dattwyler, R J, Gorevic, P D
Purified flagellar protein (p41) of Borrelia burgdorferi (strain B31) was subjected to chemical cleavage with hydroxylamine or proteolysis with V8 protease, endoproteinase Asp-N, or...
Beckers, C J, Roos, D S, Donald, R G, Luft, B J, Schwab, J C, Cao, Y, ...
We investigated potential targets for the activity of protein synthesis inhibitors against the protozoan parasite Toxoplasma gondii. Although nanomolar concentrations of azithromycin and clindamycin...
Early and specific antibody response to OspA in Lyme Disease.
Schutzer, S E, Coyle, P K, Dunn, J J, Luft, B J, Brunner, M
Borrelia burgdorferi (Bb), the cause of Lyme disease, has appeared not to evoke a detectable specific antibody response in humans until long after infection. This delayed response has been a biologic...
Studies of the effect of pregnancy on the capacity of mice to resist Listeria monocytogenes and Toxoplasma gondii infection revealed significantly diminished resistance of pregnant mice to infection...
Jiang, W, Gorevic, P D, Dattwyler, R J, Dunn, J J, Luft, B J
The major outer surface protein, OspA, of Borrelia burgdorferi is a lipoprotein which is a particular interest because of its potential as a vaccine candidate. However, serotypic and genetic analysis...
Schutzer, S E, Coyle, P K, Krupp, L B, Deng, Z, Belman, A L, Dattwyler, R, ...
Lyme disease is the major tick-borne disease, caused by Borrelia burgdorferi (Bb). Neurological involvement is common in all stages. In vivo expression of Bb antigens (Ags) and the immune response to...
Penicillin-binding proteins in Borrelia burgdorferi.
Urban, C, Rahal, J J, Dattwyller, R J, Gorevic, P, Luft, B J
Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band...
Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto.
Wang, I N, Dykhuizen, D E, Qiu, W, Dunn, J J, Bosler, E M, Luft, B J
The outer surface protein, OspC, is highly variable in Borrelia burgdorferi sensu stricto, the agent of Lyme disease. We have shown that even within a single population OspC is highly variable. The...
In vitro generation of adherent mononuclear suppressor cells to Toxoplasma antigen.
Luft, B J, Pedrotti, P W, Remington, J S
Adherent mononuclear cells have been found to suppress the lymphocyte proliferation, of T lymphocytes of patients with various chronic infections, to pathogen-specific antigens. To explore mechanisms...