Cardenas, Paula P., Carrasco, Begoña, Sanchez, Humberto, Deikus, Gintaras, Bechhofer, David H, Alonso, Juan C
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN...
The RecU Holliday junction resolvase acts at early stages of homologous recombination (2008)
Cañas, Cristina, Carrasco, Begoña, Ayora, Silvia, Alonso, Juan C.
Homologous recombination is essential for DNA repair and generation of genetic diversity in all organisms. It occurs through a series of presynaptic steps where the substrate is presented to the...
Carrasco, Begoña, Fernández, Silvia, Petit, Marie-Agnes, Alonso, Juan C.
The B. subtilis ΔhelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec+ strain. The ΔhelD...
Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA
Ayora, Silvia, Carrasco, Begoña, Doncel, Ernesto, Lurz, Rudi, Alonso, Juan C.
Bacillus subtilis RecU protein is involved in homologous recombination, DNA repair, and chromosome segregation. Purified RecU binds preferentially to three- and four-strand junctions when compared to...
Carrasco, Begoña, Cozar, M. Castillo, Lurz, Rudi, Alonso, Juan C., Ayora, Silvia
Bacillus subtilis mutants classified within the ɛ (ruvA, ΔruvB, ΔrecU, and recD) and η (ΔrecG) epistatic groups, in an otherwise rec+ background, render cells impaired in chromosomal...
Bacillus subtilis RecU Holliday-junction resolvase modulates RecA activities
Carrasco, Begoña, Ayora, Silvia, Lurz, Rudi, Alonso, Juan C.
The Bacillus subtilis RecU protein is able to catalyze in vitro DNA strand annealing and Holliday-junction resolution. The interaction between the RecA and RecU proteins, in the presence or absence...
Carrasco, Begoña, Fernández, Silvia, Petit, Marie-Agnes, Alonso, Juan C.
The B. subtilis ΔhelD allele rendered cells proficient in transformational recombination and moderately sensitive to methyl methanesulfonate when present in an otherwise Rec+ strain. The ΔhelD...
Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA
Ayora, Silvia, Carrasco, Begoña, Doncel, Ernesto, Lurz, Rudi, Alonso, Juan C.
Bacillus subtilis RecU protein is involved in homologous recombination, DNA repair, and chromosome segregation. Purified RecU binds preferentially to three- and four-strand junctions when compared to...
Carrasco, Begoña, Cozar, M. Castillo, Lurz, Rudi, Alonso, Juan C., Ayora, Silvia
Bacillus subtilis mutants classified within the ɛ (ruvA, ΔruvB, ΔrecU, and recD) and η (ΔrecG) epistatic groups, in an otherwise rec+ background, render cells impaired in chromosomal...
Bacillus subtilis RecU Holliday-junction resolvase modulates RecA activities
Carrasco, Begoña, Ayora, Silvia, Lurz, Rudi, Alonso, Juan C.
The Bacillus subtilis RecU protein is able to catalyze in vitro DNA strand annealing and Holliday-junction resolution. The interaction between the RecA and RecU proteins, in the presence or absence...
The RecU Holliday junction resolvase acts at early stages of homologous recombination
Cañas, Cristina, Carrasco, Begoña, Ayora, Silvia, Alonso, Juan C.
Homologous recombination is essential for DNA repair and generation of genetic diversity in all organisms. It occurs through a series of presynaptic steps where the substrate is presented to the...
Bacillus subtilis polynucleotide phosphorylase 3′-to-5′ DNase activity is involved in DNA repair
Cardenas, Paula P., Carrasco, Begoña, Sanchez, Humberto, Deikus, Gintaras, Bechhofer, David H, Alonso, Juan C
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN...
Evidence for Different Pathways during Horizontal Gene Transfer in Competent Bacillus subtilis Cells
Kidane, Dawit, Carrasco, Begoña, Manfredi, Candela, Rothmaier, Katharina, Ayora, Silvia, Tadesse, Serkalem, ...
Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA. The integration of homologous donor...