Towards a multiplex cereal traceability tool using padlock probe ligation on genomic DNA (2010)
Prins, T.W., Voorhuijzen, M.M., Broeders, S., Trapmann, S., ...
Current EU regulations on the protection of products with certain characteristics (geographical indications and designations of origin) aim to ensure fair competition for producers and increased...
Prins, T.W., Beenen, H.G., Voorhuijzen, M.M., Schoen, C.D., ...
Background To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised...
Monitoren van schimmelsporen (2007)
Köhl, J., Schoen, C.D., Bergervoet, J.H.W., Paternotte, S.J., ...
Monitoren van schimmelsporen : thema: effectief en duurzaam middelenpakket BO-06-004-001.003 (2007)
Köhl, J., Schoen, C.D., Bergervoet, J.H.W., Paternotte, S.J., ...
Szemes, M., Bonants, P.J.M., Kowalchuk, G.A., Salles, J.F., Ortenberg, E., ...
Background Diagnostics and disease-management strategies require technologies to enable the simultaneous detection and quantification of a wide range of pathogenic microorganisms. Most multiplex,...
Anderson, N.M., Szemes, M., O'Brien, P.A., Schoen, C.D., Boender, P.J., ...
Microarray-based detection is limited by variable and inconsistent hybridization intensities across the diversity of probes used in each array. In this paper, we introduce a novel concept for the...
Monitoren van schimmelsporen : thema effectief en duurzaam middelenpakket BO-06-004 (2006)
Köhl, J., Amsing, J.J., Schoen, C.D., Bonants, P.J.M., Bergervoet, J.H.W., ...
Szemes, M., Bonants, P.J.M., Baner, J., Landegren, U., Schoen, C.D.
Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP...
Real-time NASBA for sensitive detection of Strawberry vein banding virus (2004)
Vasková, D., Spak, J., Klerks, M.M., Schoen, C.D., Thompson, J.R., Jelkmann, W.
An assay for the detection of Strawberry vein banding virus (SVBV) in Fragaria spp. based on nucleic acid sequence based amplification (NASBA) and real-time detection using molecular beacons...
Detection and tentative grouping of Strawberry crinkle virus isolates (2004)
Klerks, M.M., Lindner, J.L., Vasková, D., Spak, J., Thompson, J.R., Jelkmann, W., ...
A partial sequence of the putative polymerase (L) protein of Strawberry crinkle virus (SCV), genus Cytorhabdovirus, is described. The virus protein was found to be distantly related to the L protein...
Quantitative detection of Fusarium species in wheat using TaqMan (2004)
Waalwijk, C., Schoen, C.D., Costrel-Decorainville, G., ...
Fusarium head blight (FHB) of wheat and other small-grain cereals is a disease complex caused by several fungal species. To monitor and quantify the major species in the FHB complex during the...
From single to multiplex detection of plant pathogens by micro-arrays (2004)
Bonants, P.J.M., Schoen, C.D., Szemes, M., Klerks, M.M., ...
AmpliDet RNA is a real-time diagnostic method, the specificity of which is defined mainly by the molecular beacon (MB). MBs can be characterized according to the stability of their stem-and-loop...
Thompson, J.R., Wetzel, S., Klerks, M.M., Vasková, D., Schoen, C.D., Spak, J., ...
The principal aphid-borne viruses infecting Strawberry (Fragaria spp.) Strawberry crinkle virus (SCV), Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV) and Strawberry vein...
Kalanchoë blossfeldiana, a new host for Sonchus yellow net virus (2002)
The agent causing chlorotic spots in Kalanchoë blossfeldiana `Isabella¿ was investigated. A virus isolated from this naturally infected kalanchoë was mechanically transmissible to several...
Szemes, M., Klerks, M.M., Schoen, C.D.
A multiplex AmpliDet RNA assay was developed for the specific detection of potato virus Y (PVY), and for the differentiation of the PVYN, PVYO/C strains and the tuber necrotic isolates (PVYNTN). The...
Szemes, M., Klerks, M.M., Schoen, C.D.
A multiplex AmpliDet RNA assay was developed for the specific detection of potato virus Y (PVY), and for the differentiation of the PVY(N), PVY(O/C) strains and the tuber necrotic isolates...
Klerks, M.M., Leone, G.O.M., Verbeek, M., Schoen, C.D.
A novel isothermal multiplex AmpliDet RNA system is described for the simultaneous amplification and detection of Potato leafroll virus (PLRV) and Potato virus Y (PVY) in seed potatoes. The risk of...
Molecular cloning of dsRNAs associated with strawberry mottle virus (1998)
Schoen, C.D., Miglino, R., Jelkmann, W., Leone, G.
Strawberry mottle virus was transmitted from Fragaria vesca to Chenopodium quinoa and Nicotiana occidentalis 37B. In all plants, SMoV infection-associated dsRNA was detected but dsRNA patterns showed...
Leone, G., Lindner, J.L., Schoen, C.D., Jongedijk, G.
Isolates of apple stem pitting virus (ASPV) from diseased apple trees were maintained in Nicotiana occidentalis then back-transmitted mechanically from the herbaceous host to apple seedlings and...
NASBA, an isothermal amplification method for nucleic acids, was applied to the detection of RNA of potato leafroll virus (PLRV) in a single enzymatic reaction at 41 °C. A set of primers was...
Towards molecular detection methods for aphid-borne strawberry viruses (1995)
Intact rhabdovirus particles of strawberry crinkle virus (SCV) mechanically transmitted from Fragaria vesca UC-S to Physalis pubescens plants were purified. When these particles were used as an...
Unstable infectivity and abundant viral RNAs associated with strawberry mottle virus (1995)
Leone, G., Lindner, J.L., Schoen, C.D.
A not yet identified virus (SMoV) associated with the strawberry mottle syndrome was mechanically transmitted from infected Fragaria vesca UC-5 to Chenopodium quinoa and from C. quinoa to Nicotiana...
Leone, G, Van Schijndel, H, Van Gemen, B, Kramer, F R, Schoen, C D
Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize...
Leone, G, Van Schijndel, H, Van Gemen, B, Kramer, F R, Schoen, C D
Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize...