C. H. Klaassen

Prevalence of methicillin-resistant Staphylococcus aureus among veterinarians: an international study (2008)

Wulf, M., Sørum, M., Nes, A. Van, Skov, R., Melchers, W.J., Klaassen, C.H., ...

Pig farmers and veterinarians in contact with livestock in The Netherlands have a higher risk of methicillin-resistant Staphylococcus aureus (MRSA) carriage than the general population. The objective...

Co-localization and functional coupling of creatine kinase B and gastric H+/K(+)-ATPase on the apical membrane and the tubulovesicular system of parietal cells.

Sistermans, E A, Klaassen, C H, Peters, W, Swarts, H G, Jap, P H, De Pont, J J, ...

Immunogold labelling of creatine kinase B (BB-CK) and gastric H+/K(+)-ATPase in the parietal cells of the stomach revealed colocalization of these two enzymes on the apical membrane and the membranes...

Glycosylation is essential for biosynthesis of functional gastric H+,K+-ATPase in insect cells.

Klaassen, C H, Fransen, J A, Swarts, H G, De Pont, J J

The role of N-linked glycosylation in the functional properties of gastric H+,K+-ATPase has been examined with tunicamycin and I-deoxymannojirimycin, inhibitors in glycoprotein biosynthesis and...

Large-scale production and purification of the human green cone pigment: characterization of late photo-intermediates.

Vissers, P M, Bovee-Geurts, P H, Portier, M D, Klaassen, C H, Degrip, W J

We present the first characterization of the late photo-intermediates (Meta I, Meta II and Meta III) of a vertebrate cone pigment in a lipid environment. Marked differences from the same pathway in...

Large-scale production and purification of functional recombinant bovine rhodopsin with the use of the baculovirus expression system.

Klaassen, C H, Bovee-Geurts, P H, Decaluwé, G L, DeGrip, W J

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged...

Co-localization and functional coupling of creatine kinase B and gastric H+/K(+)-ATPase on the apical membrane and the tubulovesicular system of parietal cells.

Sistermans, E A, Klaassen, C H, Peters, W, Swarts, H G, Jap, P H, De Pont, J J, ...

Immunogold labelling of creatine kinase B (BB-CK) and gastric H+/K(+)-ATPase in the parietal cells of the stomach revealed colocalization of these two enzymes on the apical membrane and the membranes...

Glycosylation is essential for biosynthesis of functional gastric H+,K+-ATPase in insect cells.

Klaassen, C H, Fransen, J A, Swarts, H G, De Pont, J J

The role of N-linked glycosylation in the functional properties of gastric H+,K+-ATPase has been examined with tunicamycin and I-deoxymannojirimycin, inhibitors in glycoprotein biosynthesis and...

Large-scale production and purification of the human green cone pigment: characterization of late photo-intermediates.

Vissers, P M, Bovee-Geurts, P H, Portier, M D, Klaassen, C H, Degrip, W J

We present the first characterization of the late photo-intermediates (Meta I, Meta II and Meta III) of a vertebrate cone pigment in a lipid environment. Marked differences from the same pathway in...

Large-scale production and purification of functional recombinant bovine rhodopsin with the use of the baculovirus expression system.

Klaassen, C H, Bovee-Geurts, P H, Decaluwé, G L, DeGrip, W J

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged...

Evaluation of the trophic effect of longterm treatment with the histamine H2 receptor antagonist loxtidine on rat oxyntic mucosa by differential counting of dispersed cells.

Brenna, E, Swarts, H G, Klaassen, C H, De Pont, J J, Waldum, H L

To evaluate whether the general trophic effect of gastrin on the oxyntic mucosa is an indirect effect mediated by histamine H2 receptors, sustained 24 hour hypergastrinaemia was induced in...