Gel permeation chromatography of proteins in partly aqueous eluents (1984)
Ligny, C.L. De, Gelesma, W.J., Roozen, A.M.P.
Gel permeation chromatography of a number of proteins has been performed with Sephadex G-100 and G-200, using mixtures of water and organic solvents as eluents. It appears that the chromatographic...
Ligny, C.L. De, Spanjer, M.C., Houwelingen, J.C. Van, Weesie, H.M.
It is shown that the Snyder equation is not quite satisfactory for fitting retention data in normal-phase high-performance liquid chromatography (HPLC) on chemically bonded phases. This equation is a...
Os, M.J. Van, Slanina, J., Ligny, C.L. De, Hammers, W.E., Agterdenbos, J.
Zipax-SAX pellicular beads are used as the anion-exchanger material ; a high-pressure packing technique is described. A Zipax-SAX column (200 × 4.5 mm) is used in a separation system with eluent...
Dekker, B.G., Arts, C.J.M., Ligny, C.L. De
The labeling of human serum albumin (HSA) with 99mTc using Sn(II) as the reductant has been reinvestigated in view of the possibility of formation of Sn-Tc colloids. Preparations of 99mTc-HSA and...
Hammers, W.E., Meurs, G.J., Ligny, C.L. De
Literature data on activity coefficients of various solutes in water, of some tetraalkyl compounds in methanol-water mixture and of water in organic solvents have been correlated with the product of...
Gelsema, W.J., Ligny, C.L. De, Blanken, W.M., Hamer, R.J., Roozen, A.M.P.
The merits of hydrophobic interaction chromatography on octyl-Sepharose as a method for the separation of carrier ampholytes from peptides have been investigated. K values for the association of...
Korteland, J., Dekker, B.G., Ligny, C.L. De
The valence state of technetium-99 in its complexes with bleomycin, 1-hydroxy-ethylidene-1,1-diphosphonate and human serum albumin was determined by titration of 99TcO4− with Sn(II) in the presence...
Selectivity of nucleosil 10 NH2 as an adsorbent in high-performance (1979)
Hammers, W.E., Spanjer, M.C., Ligny, C.L. De
Net retention volumes per gram of Nucleosil 10 NH2 have been measured for a large number of mono- and disubstituted benzene derivatives and of polycyclic aromatic hydrocarbons, using n-hexane,...
The specific conductivities of focused Ampholine, Servalyte and Pharmalyte carrier ampholytes have been determined and the results used to establish selection rules for carrier ampholytes in...
Isoelectric points, pIapp, in sucrose-urea-water and glycerol-ethanol-water mixtures and isoelectric points, pI, in water have been determined at 25° for some carrier ampholytes. The differences,...
Hammers, W.E., Kos, C.H., Brederode, W.K., Ligny, C.L. De
Net retention volumes per gram of N-2-cyanoethyl-N-methylaminosilica have been measured for a large number of mono- and disubstituted benzenes, some mono-substituted (cyclo)hexanes and a number of...
Determination of complex association constants from gas chromatograhic data (1975)
Ligny, C.L. De, Koole, N.J., Nelson, H.D., Nieuwdorp, G.H.E.
Abstract The equilibrium constants for hydrogen bonding of a series of β-substituted alcohols with a number of monofunctional hexadecyl derivatives and dioctyl compounds have been measured by gas...
The use of the Cerenkov effect in the counting of β- and γ- emitting radionuclides (1975)
Gelsema, W.J., Ligny, C.L. De, Luten, J.B., Vossenberg, F.G.A.
In a transparent medium, β-radiation and the electrons which are released, under certain conditions, by the interaction of γ-radiation with the medium cause Cerenkov-radiation. The intensity of...
The orientation of solvent-dipoles at the surface of the pure solvent (1975)
A method is described for the assessment of the preferential orientation of solvent-dipoles at the surface of the solvent from the surface potential χ and its temperature coefficient, dχ/dT. The...
Peak broadening in paper chromatography and related techniques (1972)
Ligny, C.L. De, Veen, N.G. Van
Peak dispersion (i.e. the separation efficiency) in thin-layer electrophoresis was investigated and compared for six different cellulose layers. The relative importance of longitudinal diffusion and...
Determination of complex association constants from gas chromatographic data (1972)
The basic expressions for the determination of complex association constants from gas chromatographic data in terms of the activities of the reactants are derived. The advantage of using a reference...
Pierotti's theory for the solubility of gases in liquids is tested by means of a large amount of literature data on solubility and entropy of solution. The involved solutes comprise the noble gases,...
The conditions under which the moving rate of a solute in chromatography is influenced by restricted diffusion are investigated. It appears that this phenomenon does not occur in practice in...
The efficiency of several chromatography papers and thin-layer cellulose powders for the separation of amino acids is investigated, using the minimum elution time for a given resolution as the...
Ligny, C.L. De, Remijnse, A.G.
The mechanism of peak broadening in thin-layer chromatography on cellulose powder was investigated by comparing the peak widths obtained in chromatography with those caused only by diffusion in the...
Ligny, C.L. De, Remijnse, A.G.
The mechanism of peak broadening in paper chromatography is investigated by comparing the peak widths obtained in chromatography with those caused only by diffusion, for a set of amino acids of...