Baum, Jake, Papenfuss, Anthony T., Mair, Gunnar R., Janse, Chris J., Vlachou, Dina, Waters, Andrew P., ...
Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria, are currently intractable for those genes that are essential for blood stage development. The ability to use...
Braks, Joanna A. M., Mair, Gunnar R., Franke-Fayard, Blandine, Janse, Chris J., Waters, Andrew P.
Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the...
A role for natural regulatory T cells in the pathogenesis of experimental cerebral malaria (2007)
Amante, Fiona H., Stanley, Amanda C., Randall, Louise M., Zhou, Yonghong, Haque, Ashraful, McSweeney, Karli, ...
Cerebral malaria (CM) is a serious complication of Plasmodium falciparum infection that is responsible for a significant number of deaths in children and nonimmune adults. A failure to control blood...
A role for natural regulatory T cells in the pathogenesis of experimental cerebral malaria (2007)
Amante, Fiona H., Stanley, Amanda C., Randall, Louise M., Zhou, Yonghong, Haque, Ashraful, McSweeney, Karli, ...
Cerebral malaria (CM) is a serious complication of Plasmodium falciparum infection that is responsible for a significant number of deaths in children and nonimmune adults. A failure to control blood...
Braks, Joanna A. M., Franke-Fayard, Blandine, Kroeze, Hans, Janse, Chris J., Waters, Andrew P.
A limitation of transfection of malaria parasites is the availability of only a low number of positive selectable markers for selection of transformed mutants. This is exacerbated for the rodent...
Jane M. Carlton, Shelby L. Bidwell, Neil Hall, Jai Ramesar, Chris J. Janse, ...
Whole-genome comparisons are highly informative regarding genome evolution and can reveal the conservation of genome organization and gene content, gene regulatory elements, and presence of...
Sakamoto, Hiroshi, Thiberge, Sabine, Akerman, Susan, Janse, Chris J., Carvalho, Teresa Gil, Ménard, Robert
After the deciphering of the genome sequences of several Plasmodium species, efforts must turn to elucidating gene function and identifying essential gene products. However, random approaches are...
Williamson, Donald H., Janse, Chris J., Moore, Peter W., Waters, Andrew P., Preiser, Peter R.
The rodent malaria Plasmodium berghei is one of a small number of species of Plasmodium that can currently be genetically transformed through experimentally controlled uptake of exogenous DNA by...
Pace, Tomasino, Janse, Chris J., Birago, Cecilia, Ramesar, Jai, Picci, Leonardo, ...
A 13.6 kb contig of chromosome 5 of Plasmodium berghei, a rodent malaria parasite, has been sequenced and analysed for its coding potential. Assembly and comparison of this genomic locus with the...
Targeted Terminal Deletions as a Tool for Functional Genomics Studies in Plasmodium (2000)
Pace, Tomasino, Scotti, Raffaella, Janse, Chris J., Waters, Andrew P., Birago, Cecilia, Ponzi, Marta
Pace, Tomasino, Janse, Chris J., Birago, Cecilia, Ramesar, Jai, Picci, Leonardo, ...
A 13.6 kb contig of chromosome 5 of Plasmodium berghei, a rodent malaria parasite, has been sequenced and analysed for its coding potential. Assembly and comparison of this genomic locus with the...
Topology and replication of a nuclear episomal plasmid in the rodent malaria Plasmodium berghei
Williamson, Donald H., Janse, Chris J., Moore, Peter W., Waters, Andrew P., Preiser, Peter R.
The rodent malaria Plasmodium berghei is one of a small number of species of Plasmodium that can currently be genetically transformed through experimentally controlled uptake of exogenous DNA by...
Margos, Gabriele, Van Dijk, Melissa R., Ramesar, Jai, Janse, Chris J., Waters, Andrew P., Sinden, Robert E.
Pbs21 is a surface protein of the ookinete of Plasmodium berghei, which can induce a potent transmission-blocking immune response. Pbs21 is normally expressed only by parasite stages in the mosquito,...
P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions
Tomas, Ana M., Margos, Gabriele, Dimopoulos, George, Sinha, Ria, ...
The ookinete surface proteins (P25 and P28) are proven antimalarial transmission-blocking vaccine targets, yet their biological functions are unknown. By using single (Sko) and double gene knock-out...
Targeted Terminal Deletions as a Tool for Functional Genomics Studies in Plasmodium
Pace, Tomasino, Scotti, Raffaella, Janse, Chris J., Waters, Andrew P., Birago, Cecilia, Ponzi, Marta
We describe a transfection system that induces terminal deletions at specific chromosome ends in malaria parasites using a linear construct containing telomeric repeats at one end and plasmodial...
Franke-Fayard, Blandine, Janse, Chris J., Cunha-Rodrigues, Margarida, Ramesar, Jai, Büscher, Philippe, Que, Ivo, ...
Sequestration of malaria-parasite-infected erythrocytes in the microvasculature of organs is thought to be a significant cause of pathology. Cerebral malaria (CM) is a major complication of...
Van Dijk, Melissa R., Douradinha, Bruno, Franke-Fayard, Blandine, Heussler, Volker, Van Dooren, Maaike W., Van Schaijk, Ben, ...
Immunization with Plasmodium sporozoites that have been attenuated by γ-irradiation or specific genetic modification can induce protective immunity against subsequent malaria infection. The...
Species-Specific Inhibition of Cerebral Malaria in Mice Coinfected with Plasmodium spp.
Voza, Tatiana, Vigário, Ana M., Belnoue, Elodie, Grüner, Anne Charlotte, Deschemin, Jean-Christophe, Kayibanda, Michèle, ...
Recent epidemiological observations suggest that clinical evolution of Plasmodium falciparum infections might be influenced by the concurrent presence of another Plasmodium species, and such...
Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis
Sakamoto, Hiroshi, Thiberge, Sabine, Akerman, Susan, Janse, Chris J., Carvalho, Teresa Gil, Ménard, Robert
After the deciphering of the genome sequences of several Plasmodium species, efforts must turn to elucidating gene function and identifying essential gene products. However, random approaches are...
Kooij, Taco W. A, Carlton, Jane M, Bidwell, Shelby L, Hall, Neil, Ramesar, Jai, Janse, Chris J, ...
Whole-genome comparisons are highly informative regarding genome evolution and can reveal the conservation of genome organization and gene content, gene regulatory elements, and presence of...
Braks, Joanna A. M., Franke-Fayard, Blandine, Kroeze, Hans, Janse, Chris J., Waters, Andrew P.
A limitation of transfection of malaria parasites is the availability of only a low number of positive selectable markers for selection of transformed mutants. This is exacerbated for the rodent...
Pace, Tomasino, Janse, Chris J., Birago, Cecilia, Ramesar, Jai, Picci, Leonardo, ...
A 13.6 kb contig of chromosome 5 of Plasmodium berghei, a rodent malaria parasite, has been sequenced and analysed for its coding potential. Assembly and comparison of this genomic locus with the...
Topology and replication of a nuclear episomal plasmid in the rodent malaria Plasmodium berghei
Williamson, Donald H., Janse, Chris J., Moore, Peter W., Waters, Andrew P., Preiser, Peter R.
The rodent malaria Plasmodium berghei is one of a small number of species of Plasmodium that can currently be genetically transformed through experimentally controlled uptake of exogenous DNA by...
Margos, Gabriele, Van Dijk, Melissa R., Ramesar, Jai, Janse, Chris J., Waters, Andrew P., Sinden, Robert E.
Pbs21 is a surface protein of the ookinete of Plasmodium berghei, which can induce a potent transmission-blocking immune response. Pbs21 is normally expressed only by parasite stages in the mosquito,...
P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions
Tomas, Ana M., Margos, Gabriele, Dimopoulos, George, Sinha, Ria, ...
The ookinete surface proteins (P25 and P28) are proven antimalarial transmission-blocking vaccine targets, yet their biological functions are unknown. By using single (Sko) and double gene knock-out...
Targeted Terminal Deletions as a Tool for Functional Genomics Studies in Plasmodium
Pace, Tomasino, Scotti, Raffaella, Janse, Chris J., Waters, Andrew P., Birago, Cecilia, Ponzi, Marta
We describe a transfection system that induces terminal deletions at specific chromosome ends in malaria parasites using a linear construct containing telomeric repeats at one end and plasmodial...
Franke-Fayard, Blandine, Janse, Chris J., Cunha-Rodrigues, Margarida, Ramesar, Jai, Büscher, Philippe, Que, Ivo, ...
Sequestration of malaria-parasite-infected erythrocytes in the microvasculature of organs is thought to be a significant cause of pathology. Cerebral malaria (CM) is a major complication of...
Van Dijk, Melissa R., Douradinha, Bruno, Franke-Fayard, Blandine, Heussler, Volker, Van Dooren, Maaike W., Van Schaijk, Ben, ...
Immunization with Plasmodium sporozoites that have been attenuated by γ-irradiation or specific genetic modification can induce protective immunity against subsequent malaria infection. The...
Species-Specific Inhibition of Cerebral Malaria in Mice Coinfected with Plasmodium spp.
Voza, Tatiana, Vigário, Ana M., Belnoue, Elodie, Grüner, Anne Charlotte, Deschemin, Jean-Christophe, Kayibanda, Michèle, ...
Recent epidemiological observations suggest that clinical evolution of Plasmodium falciparum infections might be influenced by the concurrent presence of another Plasmodium species, and such...
Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis
Sakamoto, Hiroshi, Thiberge, Sabine, Akerman, Susan, Janse, Chris J., Carvalho, Teresa Gil, Ménard, Robert
After the deciphering of the genome sequences of several Plasmodium species, efforts must turn to elucidating gene function and identifying essential gene products. However, random approaches are...
Kooij, Taco W. A, Carlton, Jane M, Bidwell, Shelby L, Hall, Neil, Ramesar, Jai, Janse, Chris J, ...
Whole-genome comparisons are highly informative regarding genome evolution and can reveal the conservation of genome organization and gene content, gene regulatory elements, and presence of...
Braks, Joanna A. M., Franke-Fayard, Blandine, Kroeze, Hans, Janse, Chris J., Waters, Andrew P.
A limitation of transfection of malaria parasites is the availability of only a low number of positive selectable markers for selection of transformed mutants. This is exacerbated for the rodent...
Augustijn, Kevin D., Kleemann, Robert, Thompson, Joanne, Kooistra, Teake, Crawford, Carina E., Reece, Sarah E., ...
Macrophage migration inhibitory factor (MIF) is a mammalian cytokine that participates in innate and adaptive immune responses. Homologues of mammalian MIF have been discovered in parasite species...
A Role for Natural Regulatory T Cells in the Pathogenesis of Experimental Cerebral Malaria
Amante, Fiona H., Stanley, Amanda C., Randall, Louise M., Zhou, Yonghong, Haque, Ashraful, McSweeney, Karli, ...
Cerebral malaria (CM) is a serious complication of Plasmodium falciparum infection that is responsible for a significant number of deaths in children and nonimmune adults. A failure to control blood...
Braks, Joanna A. M., Mair, Gunnar R., Franke-Fayard, Blandine, Janse, Chris J., Waters, Andrew P.
Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the...
Tomás, Ana M., Kocken, Clemens H.M., Malhotra, Pawan, Janse, Chris J., Waters, Andrew P., ...
The recently developed transfection systems for Plasmodium berghei and Plasmodium falciparum offer important new tools enabling further insight into the biology of malaria parasites. These systems...
Janse, Chris J., Van Gemert, Geert-Jan, Van Dijk, Melissa R., Gego, Audrey, Franetich, Jean-Francois, ...
Difficulties with inducing sterile and long lasting protective immunity against malaria with subunit vaccines has renewed interest in vaccinations with attenuated Plasmodium parasites. Immunizations...
Lasonder, Edwin, Janse, Chris J., Van Gemert, Geert-Jan, Mair, Gunnar R., Vermunt, Adriaan M. W., Douradinha, Bruno G., ...
Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite...
The Glutathione Biosynthetic Pathway of Plasmodium Is Essential for Mosquito Transmission
Vega-Rodríguez, Joel, Franke-Fayard, Blandine, Dinglasan, Rhoel R., Janse, Chris J., Pastrana-Mena, Rebecca, Waters, Andrew P., ...
Infection of red blood cells (RBC) subjects the malaria parasite to oxidative stress. Therefore, efficient antioxidant and redox systems are required to prevent damage by reactive oxygen species....
Correction: The Malaria Secretome: From Algorithms to Essential Function in Blood Stage Infection
Van Ooij, Christiaan, Tamez, Pamela, Bhattacharjee, Souvik, Hiller, N. Luisa, Harrison, Travis, Liolios, Konstantinos, ...
Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites
Baum, Jake, Papenfuss, Anthony T., Mair, Gunnar R., Janse, Chris J., Vlachou, Dina, Waters, Andrew P., ...
Techniques for targeted genetic disruption in Plasmodium, the causative agent of malaria, are currently intractable for those genes that are essential for blood stage development. The ability to use...
Vega-Rodríguez, Joel, Franke-Fayard, Blandine, Dinglasan, Rhoel R., Janse, Chris J., Pastrana-Mena, Rebecca, Waters, Andrew P., ...
A Cyclic GMP Signalling Module That Regulates Gliding Motility in a Malaria Parasite
Moon, Robert W., Taylor, Cathy J., Bex, Claudia, Schepers, Rebecca, Goulding, David, Janse, Chris J., ...
The ookinete is a motile stage in the malaria life cycle which forms in the mosquito blood meal from the zygote. Ookinetes use an acto-myosin motor to glide towards and penetrate the midgut wall to...
Visualisation and Quantitative Analysis of the Rodent Malaria Liver Stage by Real Time Imaging
Ploemen, Ivo H. J., Prudêncio, Miguel, Douradinha, Bruno G., Ramesar, Jai, Fonager, Jannik, Van Gemert, Geert-Jan, ...
The quantitative analysis of Plasmodium development in the liver in laboratory animals in cultured cells is hampered by low parasite infection rates and the complicated methods required to monitor...