Zhou, Jing, Chen, Chin, Aiken, Christopher
Abstract Background Despite the effectiveness of currently available antiretroviral therapies in the treatment of HIV-1 infection, a continuing need exists for novel compounds that can be used in...
Kaszubska, Wiweka, Aiken, Christopher, O'Connor, C.David, Gumport, Richard I.
RsrI DNA methyltransferase (M-RsrI) from Rhodobacter sphaeroides has been purified to homogeneity, and its gene cloned and sequenced. This enzyme catalyzes methylation of the same central adenine...
Aiken, Christopher, Gumport, Richard. I.
We have purified RsrI endonuclease (R·RsrI), an isoschizomer of EcoRI, from Rhodobacter sphaeroides strain 630. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis and...
Wyma, Donald J., Kotov, Alexander, Aiken, Christopher
Assembly of infectious human immunodeficiency virus type 1 (HIV-1) virions requires incorporation of the viral envelope glycoproteins gp41 and gp120. Several lines of evidence have suggested that the...
Association of Nef with the Human Immunodeficiency Virus Type 1 Core
Kotov, Alexander, Zhou, Jing, Flicker, Paula, Aiken, Christopher
Highly conserved among primate lentiviruses, the human immunodeficiency virus type 1 (HIV-1) Nef protein enhances viral infectivity by an unknown mechanism. Nef-defective virions are blocked at a...
Zhou, Jing, Aiken, Christopher
The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef stimulates viral infectivity by facilitating an early event in the HIV-1 life cycle. Although no structural or biochemical...
Chazal, Nathalie, Singer, Gregory, Aiken, Christopher, Hammarskjöld, Marie-Louise, Rekosh, David
We have investigated the effects of Nef on infectivity in the context of various viral envelope proteins. These experiments were performed with a minimal vector system where Nef is the only accessory...
Forshey, Brett M., Von Schwedler, Uta, Sundquist, Wesley I., Aiken, Christopher
Virions of human immunodeficiency virus type 1 (HIV-1) and other lentiviruses contain conical cores consisting of a protein shell composed of the viral capsid protein (CA) surrounding an internal...
Forshey, Brett M., Aiken, Christopher
The human immunodeficiency virus type 1 (HIV-1) virulence factor Nef enhances viral infectivity in single-cycle infection assays and accelerates HIV-1 replication in vitro. It has been reported that...
Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells
Tobiume, Minoru, Lineberger, Janet E., Lundquist, Christopher A., Miller, Michael D., Aiken, Christopher
The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef stimulates viral infectivity by an unknown mechanism. Recent studies have suggested that Nef may act by regulating the efficiency...
Huang, Li, Yuan, Xiong, Aiken, Christopher, Chen, Chin Ho
A class of betulinic acid derivatives was synthesized to target two critical steps in the human immunodeficiency virus type 1 (HIV-1) replication cycle, entry and maturation. Each mechanism of HIV-1...
Zhou, Jing, Yuan, Xiong, Dismuke, David, Forshey, Brett M., Lundquist, Christopher, Lee, Kuo-Hsiung, ...
Despite the effectiveness of currently available human immunodeficiency virus type 1 (HIV-1) therapies, a continuing need exists for new drugs to treat HIV-1 infection. We investigated the mechanism...
Assembly Properties of the Human Immunodeficiency Virus Type 1 CA Protein
Ganser-Pornillos, Barbie K., Von Schwedler, Uta K., Stray, Kirsten M., Aiken, Christopher, Sundquist, Wesley I.
During retroviral maturation, the CA protein oligomerizes to form a closed capsid that surrounds the viral genome. We have previously identified a series of deleterious surface mutations within human...
Wyma, Donald J., Jiang, Jiyang, Shi, Jiong, Zhou, Jing, Lineberger, Janet E., Miller, Michael D., ...
Retrovirus particles are not infectious until they undergo proteolytic maturation to form a functional core. Here we report a link between human immunodeficiency virus type 1 (HIV-1) core maturation...
Lundquist, Christopher A., Zhou, Jing, Aiken, Christopher
The Nef protein enhances human immunodeficiency virus type 1 (HIV-1) replication through an unknown mechanism. We and others have previously reported that efficient HIV-1 replication in activated...
Forshey, Brett M., Shi, Jiong, Aiken, Christopher
Human immunodeficiency virus type 1 (HIV-1) infection of simian cells is restricted at an early postentry step by host factors whose mechanism of action is unclear. These factors target the viral...
VanCompernolle, Scott E., Taylor, R. Jeffery, Oswald-Richter, Kyra, Jiang, Jiyang, Youree, Bryan E., Bowie, John H., ...
Topical antimicrobicides hold great promise in reducing human immunodeficiency virus (HIV) transmission. Amphibian skin provides a rich source of broad-spectrum antimicrobial peptides including some...
Lundquist, Christopher A., Tobiume, Minoru, Zhou, Jing, Unutmaz, Derya, Aiken, Christopher
The accessory protein Nef plays a crucial role in primate lentivirus pathogenesis. Nef enhances human immunodeficiency virus type 1 (HIV-1) infectivity in culture and stimulates viral replication in...
Davis, Melody R., Jiang, Jiyang, Zhou, Jing, Freed, Eric O., Aiken, Christopher
The Gag protein of human immunodeficiency virus type 1 (HIV-1) associates with the envelope protein complex during virus assembly. The available evidence indicates that this interaction involves...
Dismuke, David J., Aiken, Christopher
Human immunodeficiency virus type 1 (HIV-1) particles begin their replication upon fusion with the plasma membrane of target cells and release of the viral core into the host cell cytoplasm. Soon...
Wyma, Donald J., Kotov, Alexander, Aiken, Christopher
Assembly of infectious human immunodeficiency virus type 1 (HIV-1) virions requires incorporation of the viral envelope glycoproteins gp41 and gp120. Several lines of evidence have suggested that the...
Association of Nef with the Human Immunodeficiency Virus Type 1 Core
Kotov, Alexander, Zhou, Jing, Flicker, Paula, Aiken, Christopher
Highly conserved among primate lentiviruses, the human immunodeficiency virus type 1 (HIV-1) Nef protein enhances viral infectivity by an unknown mechanism. Nef-defective virions are blocked at a...
Zhou, Jing, Aiken, Christopher
The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef stimulates viral infectivity by facilitating an early event in the HIV-1 life cycle. Although no structural or biochemical...
Chazal, Nathalie, Singer, Gregory, Aiken, Christopher, Hammarskjöld, Marie-Louise, Rekosh, David
We have investigated the effects of Nef on infectivity in the context of various viral envelope proteins. These experiments were performed with a minimal vector system where Nef is the only accessory...
Forshey, Brett M., Von Schwedler, Uta, Sundquist, Wesley I., Aiken, Christopher
Virions of human immunodeficiency virus type 1 (HIV-1) and other lentiviruses contain conical cores consisting of a protein shell composed of the viral capsid protein (CA) surrounding an internal...
Forshey, Brett M., Aiken, Christopher
The human immunodeficiency virus type 1 (HIV-1) virulence factor Nef enhances viral infectivity in single-cycle infection assays and accelerates HIV-1 replication in vitro. It has been reported that...
Lundquist, Christopher A., Tobiume, Minoru, Zhou, Jing, Unutmaz, Derya, Aiken, Christopher
The accessory protein Nef plays a crucial role in primate lentivirus pathogenesis. Nef enhances human immunodeficiency virus type 1 (HIV-1) infectivity in culture and stimulates viral replication in...
Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells
Tobiume, Minoru, Lineberger, Janet E., Lundquist, Christopher A., Miller, Michael D., Aiken, Christopher
The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef stimulates viral infectivity by an unknown mechanism. Recent studies have suggested that Nef may act by regulating the efficiency...
Huang, Li, Yuan, Xiong, Aiken, Christopher, Chen, Chin Ho
A class of betulinic acid derivatives was synthesized to target two critical steps in the human immunodeficiency virus type 1 (HIV-1) replication cycle, entry and maturation. Each mechanism of HIV-1...
Zhou, Jing, Yuan, Xiong, Dismuke, David, Forshey, Brett M., Lundquist, Christopher, Lee, Kuo-Hsiung, ...
Despite the effectiveness of currently available human immunodeficiency virus type 1 (HIV-1) therapies, a continuing need exists for new drugs to treat HIV-1 infection. We investigated the mechanism...
Assembly Properties of the Human Immunodeficiency Virus Type 1 CA Protein
Ganser-Pornillos, Barbie K., Von Schwedler, Uta K., Stray, Kirsten M., Aiken, Christopher, Sundquist, Wesley I.
During retroviral maturation, the CA protein oligomerizes to form a closed capsid that surrounds the viral genome. We have previously identified a series of deleterious surface mutations within human...
Wyma, Donald J., Jiang, Jiyang, Shi, Jiong, Zhou, Jing, Lineberger, Janet E., Miller, Michael D., ...
Retrovirus particles are not infectious until they undergo proteolytic maturation to form a functional core. Here we report a link between human immunodeficiency virus type 1 (HIV-1) core maturation...
Lundquist, Christopher A., Zhou, Jing, Aiken, Christopher
The Nef protein enhances human immunodeficiency virus type 1 (HIV-1) replication through an unknown mechanism. We and others have previously reported that efficient HIV-1 replication in activated...
Forshey, Brett M., Shi, Jiong, Aiken, Christopher
Human immunodeficiency virus type 1 (HIV-1) infection of simian cells is restricted at an early postentry step by host factors whose mechanism of action is unclear. These factors target the viral...
VanCompernolle, Scott E., Taylor, R. Jeffery, Oswald-Richter, Kyra, Jiang, Jiyang, Youree, Bryan E., Bowie, John H., ...
Topical antimicrobicides hold great promise in reducing human immunodeficiency virus (HIV) transmission. Amphibian skin provides a rich source of broad-spectrum antimicrobial peptides including some...
Davis, Melody R., Jiang, Jiyang, Zhou, Jing, Freed, Eric O., Aiken, Christopher
The Gag protein of human immunodeficiency virus type 1 (HIV-1) associates with the envelope protein complex during virus assembly. The available evidence indicates that this interaction involves...
Dismuke, David J., Aiken, Christopher
Human immunodeficiency virus type 1 (HIV-1) particles begin their replication upon fusion with the plasma membrane of target cells and release of the viral core into the host cell cytoplasm. Soon...
Zhou, Jing, Chen, Chin Ho, Aiken, Christopher
The compound 3-O-(3′,3′-dimethylsuccinyl)-betulinic acid (DSB) potently and specifically inhibits human immunodeficiency virus type 1 (HIV-1) replication by delaying the cleavage of the CA-SP1...
Qi, Mingli, Aiken, Christopher
The Nef protein enhances human immunodeficiency virus type 1 (HIV-1) infectivity by facilitating an early postentry step in the virus life cycle. We report here that the addition of MG132 or...
Yang, Ruifeng, Aiken, Christopher
The replication of many isolates of human immunodeficiency virus type 1 (HIV-1) is enhanced by binding of the host cell protein cyclophilin A (CypA) to the viral capsid protein (CA). The...
Jiang, Jiyang, Aiken, Christopher
Lentiviruses, including human immunodeficiency virus type 1 (HIV-1), typically encode fusion glycoproteins with long cytoplasmic tails (CTs). We previously reported that immature HIV-1 particles are...
Song, Chisu, Aiken, Christopher
The host cell protein cyclophilin A (CypA) binds to CA of human immunodeficiency virus type 1 (HIV-1) and promotes HIV-1 infection of target cells. Disruption of the CypA-CA interaction, either by...
Schneidewind, Arne, Brockman, Mark A., Yang, Ruifeng, Adam, Rahma I., Li, Bin, Le Gall, Sylvie, ...
Human leukocyte antigen (HLA)-B27-positive subjects are uncommon in their ability to control infection with human immunodeficiency virus type 1 (HIV-1). However, late viral escape from a narrowly...
Proteasomal Degradation of TRIM5α during Retrovirus Restriction
Rold, Christopher James, Aiken, Christopher
The host protein TRIM5α inhibits retroviral infection at an early post-penetration stage by targeting the incoming viral capsid. While the detailed mechanism of restriction remains unclear, recent...
Langelier, Charles R., Sandrin, Virginie, Eckert, Debra M., Christensen, Devin E., Chandrasekaran, Viswanathan, Alam, Steven L., ...
The rhesus monkey intrinsic immunity factor TRIM5αrh recognizes incoming capsids from a variety of retroviruses, including human immunodeficiency virus type 1 (HIV-1) and equine infectious anemia...
Qi, Mingli, Yang, Ruifeng, Aiken, Christopher
Among retroviruses, lentiviruses are unusual in their ability to efficiently infect both dividing and nondividing cells, such as activated T cells and macrophages, respectively. Recent studies...