RNase J1 endonuclease activity as a probe of RNA secondary structure (2009)
Daou-Chabo, Roula, Condon, Ciarán
Reliable determination of RNA secondary structure depends on both computer algorithms and experimental probing of nucleotides in single- or double-stranded conformation. Here we describe the...
Real-time fluorescence detection of exoribonucleases (2009)
Sinturel, Flore, Pellegrini, Olivier, Xiang, Song, Tong, Liang, Condon, Ciarán, Bénard, Lionel
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic...
The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member (2005)
Allemand, Frédéric, Mathy, Nathalie, Brechemier-Baey, Dominique, Condon, Ciarán
The maturation of 5S ribosomal RNA in low G+C Gram-positive bacteria is catalyzed by a highly conserved, ∼190 residue, enzyme, called ribonuclease M5 (RNase M5). Sequence alignment had predicted...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Transfer RNA-mediated antitermination in vitro (2002)
Putzer, Harald, Condon, Ciarán, Brechemier-Baey, Dominique, Brito, Renata, Grunberg-Manago, Marianne
The threonyl‐tRNA synthetase gene (thrS) is a member of the T‐box family of ∼250 genes, found essentially in Gram‐positive bacteria, regulated by a tRNA‐dependent...
The phylogenetic distribution of bacterial ribonucleases (2002)
Condon, Ciarán, Putzer, Harald
Ribonucleases play key, often essential, roles in cellular metabolism. Nineteen ribonuclease activities, from 22 different proteins, have so far been described in bacteria, the majority of them from...
Asai, Tsuneaki, Condon, Ciarán, Voulgaris, Justina, Zaporojets, Dmitry, Shen, Binghua, Al-Omar, Michaal, ...
The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of...
Torres, Martha, Condon, Ciarán, Balada, Joan-Miquel, Squires, Craig, Squires, Catherine L.
Escherichia coli ribosomal RNA (rRNA) operons contain antitermination motifs necessary for forming terminator-resistant transcription complexes. In preliminary work, we isolated ‘antiterminating’...
Ribonuclease M5 Has Few, If Any, mRNA Substrates in Bacillus subtilis
Condon, Ciarán, Rourera, Jordi, Brechemier-Baey, Dominique, Putzer, Harald
In Bacillus subtilis, maturation of 5S rRNA is catalyzed by an enzyme called RNase M5. We searched for potential mRNA substrates for RNase M5 by gene array technology, based on the premise that most...
Transfer RNA-mediated antitermination in vitro
Putzer, Harald, Condon, Ciarán, Brechemier-Baey, Dominique, Brito, Renata, Grunberg-Manago, Marianne
The threonyl-tRNA synthetase gene (thrS) is a member of the T-box family of ∼250 genes, found essentially in Gram-positive bacteria, regulated by a tRNA-dependent antitermination mechanism in...
SURVEY AND SUMMARY: The phylogenetic distribution of bacterial ribonucleases
Condon, Ciarán, Putzer, Harald
Ribonucleases play key, often essential, roles in cellular metabolism. Nineteen ribonuclease activities, from 22 different proteins, have so far been described in bacteria, the majority of them from...
RNA Processing and Degradation in Bacillus subtilis
This review focuses on the enzymes and pathways of RNA processing and degradation in Bacillus subtilis, and compares them to those of its gram-negative counterpart, Escherichia coli. A comparison of...
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis
Pellegrini, Olivier, Nezzar, Jamel, Marchfelder, Anita, Putzer, Harald, Condon, Ciarán
In contrast to Escherichia coli, where the 3′ ends of tRNAs are primarily generated by exoribonucleases, maturation of the 3′ end of tRNAs is catalysed by an endoribonuclease, known as RNase Z...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member
Allemand, Frédéric, Mathy, Nathalie, Brechemier-Baey, Dominique, Condon, Ciarán
The maturation of 5S ribosomal RNA in low G+C Gram-positive bacteria is catalyzed by a highly conserved, ∼190 residue, enzyme, called ribonuclease M5 (RNase M5). Sequence alignment had predicted...
Asai, Tsuneaki, Condon, Ciarán, Voulgaris, Justina, Zaporojets, Dmitry, Shen, Binghua, Al-Omar, Michaal, ...
The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of...
Torres, Martha, Condon, Ciarán, Balada, Joan-Miquel, Squires, Craig, Squires, Catherine L.
Escherichia coli ribosomal RNA (rRNA) operons contain antitermination motifs necessary for forming terminator-resistant transcription complexes. In preliminary work, we isolated ‘antiterminating’...
Ribonuclease M5 Has Few, If Any, mRNA Substrates in Bacillus subtilis
Condon, Ciarán, Rourera, Jordi, Brechemier-Baey, Dominique, Putzer, Harald
In Bacillus subtilis, maturation of 5S rRNA is catalyzed by an enzyme called RNase M5. We searched for potential mRNA substrates for RNase M5 by gene array technology, based on the premise that most...
Transfer RNA-mediated antitermination in vitro
Putzer, Harald, Condon, Ciarán, Brechemier-Baey, Dominique, Brito, Renata, Grunberg-Manago, Marianne
The threonyl-tRNA synthetase gene (thrS) is a member of the T-box family of ∼250 genes, found essentially in Gram-positive bacteria, regulated by a tRNA-dependent antitermination mechanism in...
SURVEY AND SUMMARY: The phylogenetic distribution of bacterial ribonucleases
Condon, Ciarán, Putzer, Harald
Ribonucleases play key, often essential, roles in cellular metabolism. Nineteen ribonuclease activities, from 22 different proteins, have so far been described in bacteria, the majority of them from...
RNA Processing and Degradation in Bacillus subtilis
This review focuses on the enzymes and pathways of RNA processing and degradation in Bacillus subtilis, and compares them to those of its gram-negative counterpart, Escherichia coli. A comparison of...
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis
Pellegrini, Olivier, Nezzar, Jamel, Marchfelder, Anita, Putzer, Harald, Condon, Ciarán
In contrast to Escherichia coli, where the 3′ ends of tRNAs are primarily generated by exoribonucleases, maturation of the 3′ end of tRNAs is catalysed by an endoribonuclease, known as RNase Z...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member
Allemand, Frédéric, Mathy, Nathalie, Brechemier-Baey, Dominique, Condon, Ciarán
The maturation of 5S ribosomal RNA in low G+C Gram-positive bacteria is catalyzed by a highly conserved, ∼190 residue, enzyme, called ribonuclease M5 (RNase M5). Sequence alignment had predicted...
Type A and B RNase P RNAs are interchangeable in vivo despite substantial biophysical differences
Wegscheid, Barbara, Condon, Ciarán, Hartmann, Roland K
We show that structural type A and B bacterial ribonuclease P (RNase P) RNAs can fully replace each other in vivo despite the many reported differences in their biogenesis, biochemical/biophysical...
Deikus, Gintaras, Condon, Ciarán, Bechhofer, David H.
The 140-nucleotide trp leader RNA, which is formed by transcription termination under conditions of high intracellular tryptophan, was used to study RNA turnover in Bacillus subtilis. We showed in...