Stefanovic, B., Lindquist, J., Brenner, D. A.
The stability of collagen α1(I) mRNA is regulated by its 5′ stem–loop, which binds a cytoplasmic protein in a cap-dependent manner, and its 3′-untranslated region (UTR), which binds αCP. When...
Westwick, J K, Cox, A D, Der, C J, Cobb, M H, Hibi, M, Karin, M, ...
c-Jun transcriptional activity is augmented by expression of oncogenic Ras and Raf proteins. This study demonstrates a direct correlation between Ras transforming activity and c-Jun activation,...
Cloning of murine ferrochelatase.
Ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1) catalyzes the last step in the heme biosynthetic pathway, the chelation of ferrous iron and protoporphyrin to form heme. The activity of...
Stefanovic, B., Lindquist, J., Brenner, D. A.
The stability of collagen α1(I) mRNA is regulated by its 5′ stem–loop, which binds a cytoplasmic protein in a cap-dependent manner, and its 3′-untranslated region (UTR), which binds αCP. When...
Regulatory Role of the Conserved Stem-Loop Structure at the 5′ End of Collagen α1(I) mRNA
Stefanovic, B., Hellerbrand, C., Brenner, D. A.
Three fibrillar collagen mRNAs, α1(I), α2(I), and α1(III), are coordinately upregulated in the activated hepatic stellate cell (hsc) in liver fibrosis. These three mRNAs contain sequences...
Posttranscriptional regulation of collagen alpha1(I) mRNA in hepatic stellate cells.
Stefanovic, B, Hellerbrand, C, Holcik, M, Briendl, M, Aliebhaber, S, Brenner, D A
The hepatic stellate cell (HSC) is the primary cell responsible for the dramatic increase in the synthesis of type I collagen in the cirrhotic liver. Quiescent HSCs contain a low level of collagen...
Roebuck, K A, Brenner, D A, Kagnoff, M F
Activation of HIV-1 requires the binding of host cell transcription factors to cis elements in the proviral long terminal repeat (LTR). This study identifies c-fos-responsive sequence motifs in the...
Mithramycin selectively inhibits collagen-alpha 1(I) gene expression in human fibroblast.
Nehls, M C, Brenner, D A, Gruss, H J, Dierbach, H, Mertelsmann, R, Herrmann, F
The products of the collagen-alpha 1(I) and -alpha 2(I) genes form the triple helical molecule collagen type I, which constitutes the major ECM protein in tissue fibrosis. The collagen-alpha 1(I)...
Activation of Jun kinase is an early event in hepatic regeneration.
Westwick, J K, Weitzel, C, Leffert, H L, Brenner, D A
Compensatory hepatic regeneration after partial hepatectomy (PH) is dependent upon the extent of resection. This study analyzes the regulation of the AP-1 transcription factor c-Jun during hepatic...
Tumor necrosis factor-alpha inhibits albumin gene expression in a murine model of cachexia.
Brenner, D A, Buck, M, Feitelberg, S P, Chojkier, M
The mechanisms responsible for decreased serum albumin levels in patients with cachexia-associated infection, inflammation, and cancer are unknown. Since tumor necrosis factor-alpha (TNF alpha) is...
Houglum, K, Brenner, D A, Chojkier, M
Ascorbic acid stimulates collagen gene transcription in cultured fibroblasts, and this effect is mediated through the induction of lipid peroxidation by ascorbic acid. Quiescent cultured fibroblasts...
Analysis of the collagen alpha 1(I) promoter.
Brenner, D A, Rippe, R A, Veloz, L
The collagen alpha 1(I) gene is regulated at a developmental and tissue specific level. We have previously demonstrated that only 220bp of the promoter region of the collagen alpha 1(I) gene are...
Double-strand gap repair results in homologous recombination in mouse L cells.
Brenner, D A, Smigocki, A C, Camerini-Otero, R D
Previous studies have demonstrated that the presence of double-strand breaks or double-strand gaps increases the frequency of homologous recombination between two cotransferred DNAs when they are...
DNA-mediated gene transfer into adult rat hepatocytes in primary culture.
Rippe, R A, Brenner, D A, Leffert, H L
Proliferation-competent and differentiation-competent adult rat hepatocytes in primary culture were investigated for their ability to express reporter genes (firefly luciferase, bacterial...
Nehls, M C, Rippe, R A, Veloz, L, Brenner, D A
The collagen alpha 1(I) promoter, which is efficiently transcribed in NIH 3T3 fibroblasts, contains four binding sites for trans-acting factors, as demonstrated by DNase I protection assays (D. A....
Rippe, R A, Lorenzen, S I, Brenner, D A, Breindl, M
We have identified two blocks of regulatory sequences located in the 5'-flanking region and the first intron of the mouse alpha 1 type I collagen (COL1A1) gene. Both blocks were found to contain...
Brenner, D A, Smigocki, A C, Camerini-Otero, R D
We have used DNA-mediated gene transfer to study homologous recombination in cultured mammalian cells. A family of plasmids with insertion and deletion mutations in the coding region of the herpes...
Bloomer, J R, Brenner, D A, Mahoney, M J
The activity of heme synthetase, which catalyzes the chelation of ferrous iron to protoporphyrin to form heme, is deficient in sonicates of skin fibroblasts cultured from patients with...
Mallat, A, Préaux, A M, Raufaste, D, Gallois, C, Brenner, D A, ...
During chronic liver diseases, hepatic stellate cells (HSC) acquire an activated myofibroblast-like phenotype, proliferate, and synthetize fibrosis components. We have shown that endothelin-1 (ET-1)...
NFkappaB prevents apoptosis and liver dysfunction during liver regeneration.
Iimuro, Y, Nishiura, T, Hellerbrand, C, Behrns, K E, Schoonhoven, R, Grisham, J W, ...
Although NFkappaB binding activity is induced during liver regeneration after partial hepatectomy, the physiological consequence of this induction is unknown. We have assessed the role of NFkappaB...
DNA methylation represses the murine alpha 1(I) collagen promoter by an indirect mechanism.
Rhodes, K, Rippe, R A, Umezawa, A, Nehls, M, Brenner, D A, Breindl, M
Several lines of evidence indicate that DNA methylation plays a role in the transcriptional regulation of the murine alpha 1(I) collagen gene. To study the molecular mechanisms involved, a reporter...
A molecular defect in human protoporphyria.
Brenner, D A, Didier, J M, Frasier, F, Christensen, S R, Evans, G A, Dailey, H A
Protoporphyria is generally an autosomal dominant disease that is characterized clinically by photosensitivity and hepatobiliary disease and that is characterized biochemically by elevated...
Westwick, J K, Cox, A D, Der, C J, Cobb, M H, Hibi, M, Karin, M, ...
c-Jun transcriptional activity is augmented by expression of oncogenic Ras and Raf proteins. This study demonstrates a direct correlation between Ras transforming activity and c-Jun activation,...
Cloning of murine ferrochelatase.
Ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1) catalyzes the last step in the heme biosynthetic pathway, the chelation of ferrous iron and protoporphyrin to form heme. The activity of...
Stefanovic, B., Lindquist, J., Brenner, D. A.
The stability of collagen α1(I) mRNA is regulated by its 5′ stem–loop, which binds a cytoplasmic protein in a cap-dependent manner, and its 3′-untranslated region (UTR), which binds αCP. When...
Regulatory Role of the Conserved Stem-Loop Structure at the 5′ End of Collagen α1(I) mRNA
Stefanovic, B., Hellerbrand, C., Brenner, D. A.
Three fibrillar collagen mRNAs, α1(I), α2(I), and α1(III), are coordinately upregulated in the activated hepatic stellate cell (hsc) in liver fibrosis. These three mRNAs contain sequences...
Posttranscriptional regulation of collagen alpha1(I) mRNA in hepatic stellate cells.
Stefanovic, B, Hellerbrand, C, Holcik, M, Briendl, M, Aliebhaber, S, Brenner, D A
The hepatic stellate cell (HSC) is the primary cell responsible for the dramatic increase in the synthesis of type I collagen in the cirrhotic liver. Quiescent HSCs contain a low level of collagen...
Roebuck, K A, Brenner, D A, Kagnoff, M F
Activation of HIV-1 requires the binding of host cell transcription factors to cis elements in the proviral long terminal repeat (LTR). This study identifies c-fos-responsive sequence motifs in the...
Mithramycin selectively inhibits collagen-alpha 1(I) gene expression in human fibroblast.
Nehls, M C, Brenner, D A, Gruss, H J, Dierbach, H, Mertelsmann, R, Herrmann, F
The products of the collagen-alpha 1(I) and -alpha 2(I) genes form the triple helical molecule collagen type I, which constitutes the major ECM protein in tissue fibrosis. The collagen-alpha 1(I)...
Activation of Jun kinase is an early event in hepatic regeneration.
Westwick, J K, Weitzel, C, Leffert, H L, Brenner, D A
Compensatory hepatic regeneration after partial hepatectomy (PH) is dependent upon the extent of resection. This study analyzes the regulation of the AP-1 transcription factor c-Jun during hepatic...
Tumor necrosis factor-alpha inhibits albumin gene expression in a murine model of cachexia.
Brenner, D A, Buck, M, Feitelberg, S P, Chojkier, M
The mechanisms responsible for decreased serum albumin levels in patients with cachexia-associated infection, inflammation, and cancer are unknown. Since tumor necrosis factor-alpha (TNF alpha) is...
Houglum, K, Brenner, D A, Chojkier, M
Ascorbic acid stimulates collagen gene transcription in cultured fibroblasts, and this effect is mediated through the induction of lipid peroxidation by ascorbic acid. Quiescent cultured fibroblasts...
Analysis of the collagen alpha 1(I) promoter.
Brenner, D A, Rippe, R A, Veloz, L
The collagen alpha 1(I) gene is regulated at a developmental and tissue specific level. We have previously demonstrated that only 220bp of the promoter region of the collagen alpha 1(I) gene are...
Double-strand gap repair results in homologous recombination in mouse L cells.
Brenner, D A, Smigocki, A C, Camerini-Otero, R D
Previous studies have demonstrated that the presence of double-strand breaks or double-strand gaps increases the frequency of homologous recombination between two cotransferred DNAs when they are...
DNA methylation represses the murine alpha 1(I) collagen promoter by an indirect mechanism.
Rhodes, K, Rippe, R A, Umezawa, A, Nehls, M, Brenner, D A, Breindl, M
Several lines of evidence indicate that DNA methylation plays a role in the transcriptional regulation of the murine alpha 1(I) collagen gene. To study the molecular mechanisms involved, a reporter...
DNA-mediated gene transfer into adult rat hepatocytes in primary culture.
Rippe, R A, Brenner, D A, Leffert, H L
Proliferation-competent and differentiation-competent adult rat hepatocytes in primary culture were investigated for their ability to express reporter genes (firefly luciferase, bacterial...
Nehls, M C, Rippe, R A, Veloz, L, Brenner, D A
The collagen alpha 1(I) promoter, which is efficiently transcribed in NIH 3T3 fibroblasts, contains four binding sites for trans-acting factors, as demonstrated by DNase I protection assays (D. A....
Rippe, R A, Lorenzen, S I, Brenner, D A, Breindl, M
We have identified two blocks of regulatory sequences located in the 5'-flanking region and the first intron of the mouse alpha 1 type I collagen (COL1A1) gene. Both blocks were found to contain...
Brenner, D A, Smigocki, A C, Camerini-Otero, R D
We have used DNA-mediated gene transfer to study homologous recombination in cultured mammalian cells. A family of plasmids with insertion and deletion mutations in the coding region of the herpes...
Bloomer, J R, Brenner, D A, Mahoney, M J
The activity of heme synthetase, which catalyzes the chelation of ferrous iron to protoporphyrin to form heme, is deficient in sonicates of skin fibroblasts cultured from patients with...
Mallat, A, Préaux, A M, Raufaste, D, Gallois, C, Brenner, D A, ...
During chronic liver diseases, hepatic stellate cells (HSC) acquire an activated myofibroblast-like phenotype, proliferate, and synthetize fibrosis components. We have shown that endothelin-1 (ET-1)...
NFkappaB prevents apoptosis and liver dysfunction during liver regeneration.
Iimuro, Y, Nishiura, T, Hellerbrand, C, Behrns, K E, Schoonhoven, R, Grisham, J W, ...
Although NFkappaB binding activity is induced during liver regeneration after partial hepatectomy, the physiological consequence of this induction is unknown. We have assessed the role of NFkappaB...
A molecular defect in human protoporphyria.
Brenner, D A, Didier, J M, Frasier, F, Christensen, S R, Evans, G A, Dailey, H A
Protoporphyria is generally an autosomal dominant disease that is characterized clinically by photosensitivity and hepatobiliary disease and that is characterized biochemically by elevated...
Analysis of RNA transcripts for HLA class II genes in human small intestinal biopsies.
Volk, B A, Brenner, D A, Kagnoff, M F
Studies of the expression of selected genes within the intestinal mucosa will provide important new information about physiologic pathological processes that effect mucosal growth, differentiation,...
Sanz, S, Pucilowska, J B, Liu, S, Rodríguez-Ortigosa, C M, Lund, P K, Brenner, D A, ...
Background/Aim: Hepatic stellate cells (HSCs) express α-smooth muscle actin (αSMA) and acquire a profibrogenic phenotype upon activation by noxious stimuli. Insulin-like growth I (IGF-I) has been...
Comparison of human and bovine protoporphyria.
Brenner, D. A., Bloomer, J. R.
Protoporphyria (PP) is an inherited disorder of porphyrin metabolism in man in which there is excessive accumulation and excretion of protoporphyrin. Recently, a similar disorder has been described...