Boosting tandem affinity purification of plant protein complexes (2008)
Van Leene, J, Witters, E, Inze, D, De Jaeger, G
Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few...
Trehalose-6-P synthase AtTPS1 high molecular weight complexes in yeast and Arabidopsis (2007)
Geelen, D, Royackers, K, Vanstraelen, M, De Bus, M, Inze, D, Van Dijck, P, ...
The trehalose-6-phosphate synthase AtTPS I is involved in regulating sugar metabolism and partitioning in connection with plant morphogenesis and development in an as yet unknown fashion. AtTPS I...
Van Nieuwerburgh, F, Casteele, S, Maes, L, Goossens, A, Inze, D, Van Bocxlaer, J, ...
This study reports the development and validation of a rapid, sensitive and selective assay for the quantitation of artemisinin, arteannuin B, artemisitene and artemisinic acid in Artemisia annua L....
Mitosis-specific kinesins in Arabidopsis (2006)
Vanstraelen, M, Inze, D, Geelen, D
Kinesins are a class of microtubule-associated proteins that posses a motor domain for binding to microtubulles and, in general, allows movement along microtubulles. In animal mitosis, they function...
Vanstraelen, M, Van Damme, D, De Rycke, R, Mylle, E, Inze, D, Geelen, D
Eukaryotic cells have developed different mechanisms to establish the division plane [1]. In plants, the position is determined before the onset of mitosis by the preprophase band (PPB) [2, 3]. This...
Van Damme, D, Coutuer, S, De Rycke, R, Bouget, F, Inze, D, Geelen, D
TPLATE was previously identified as a potential cytokinesis protein targeted to the cell plate. Disruption of TPLATE in Arabidopsis thaliana leads to the production of shriveled pollen unable to...
Designing factories for biopharmaceutical production by genetic engineering of plant cells (2006)
Goossens, A., Pauwels, L., Gonzalez-Guzman, M., Tilleman, S., De Sutter, V., Pollier, J., ...
FinMed 2006. Saariselkä, Finland, 27 - 31 March 2006, 16
The Solucel® technology for the production of phytopharmaceuticals (2006)
Rischer, Heiko, Schwarz, S., Goossens, A., Inze, D., Nohynek, Liisa, Oresic, Matej, ...
FinMed 2006. Saariselkä, Finland, 27 - 31 March 2006, 22
Designing factories for biopharmaceutical production by genetic engineering of plant cells (2006)
Goossens, A., Pauwels, L., Gonzalez-Guzman, M., Tilleman, S., De Sutter, V., Pollier, J., ...
FinMed 2006. Saariselkä, Finland, 27 - 31 March 2006, 16
The role of the cell cycle machinery in resumption of postembryonic development (2005)
Barroco, R.M., Van Poucke, K., Bergervoet, J.H.W., De Veylder, L., Groot, S.P.C., Inze, D., ...
Cell cycle activity is required for plant growth and development, but its involvement in the early events that initiate seedling development remains to be clarified. We performed experiments aimed at...
Switching the cell cycle. Kip-related proteins in plant cell cycle control (2005)
Verkest, A., Weinl, C., Inze, D., De Veylder, L., Schnittger, A.
Quantitative cDNA-AFLP analysis for genome-wide expression studies (2003)
Cannoot, B, Rombaut, D, Vandepoele, K, ...
An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative...
Genome-wide analysis of core cell cycle genes in Arabidopsis (2002)
Vandepoele, K, Raes, J, De Veylder, L, Rouze, P, Rombauts, S, Inze, D
Cyclin-dependent kinases and cyclins regulate with the help of different interacting proteins the progression through the eukaryotic cell cycle. A high-quality, homology-based annotation protocol was...
Van Montagu, M, Inze, D, Sanmartin, M, Kanellis, A, Smirnoff, N, ...
Humans are unable to synthesise L-ascorbic acid (L-AA, ascorbate, vitamin C), and are thus entirely dependent upon dietary sources to meet needs. In both plant and animal metabolism, the biological...
Cloning of B-1,3-glucanases expressed during Cichorium somatic embryogenesis (2000)
Helleboid, S., Chapman, A., Hendriks, T., Inze, D., Vasseur, J., Hilbert, J.L.
Three different β-1,3-glucanase cDNA fragments, CG1, CG2 and CG3, were obtained by RT-PCR from RNA isolated from Cichorium hybrid `474' leaf fragments cultured for 11 days under somatic...
Thorin, D, Hua, XJ, Mauro, S, Van Montagu, M, ...
To gain better insights into the generation of oxidative stress by NaCl stress, we compared the effects of toxic, sublethal concentrations of NaCl (150 mM) with those of CuSO4 (100 mu M) On the...
Tolerance to low temperature and paraquat-mediated oxidative stress in two maize genotypes (1999)
Iannelli, M, Van Breusegem, F, Van Montagu, M, Inze, D, Massacci, A
The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter (1998)
Storozhenko, S, De Pauw, P, Van Montagu, M, Inze, D, Kushnir, S
Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the...
Higher plants possess two structurally different poly(ADP-ribose) polymerases (1998)
Babiychuk, E, Cottrill, P, Storozhenko, S, Fuangthong, M, Chen, Y, O'Farrell, M, ...
One of the immediate reactions of the mammalian cell to many environmental stresses is a massive synthesis of poly(ADP-ribose), catalyzed by poly(ADP-ribose) polymerase (PARP). Most of the biological...
Catalase is a sink for H2O2 and is indispensable for stress defence in C-3 plants (1997)
Chamnongpol, S, Schraudner, M, Langebartels, C, VanMontagu, M, ...
Hydrogen peroxide (H2O2) has been implicated in many stress conditions, Control of H2O2 levels is complex and dissection of mechanisms generating and relieving H2O2 stress is difficult, particularly...
Baucher, M, Chabbert, B, Pilate, G, VanDoorsselaere, J, Tollier, M, PetitConil, M, ...
Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in the biosynthesis of the lignin precursors, the monolignols. We have down-regulated CAD in transgenic poplar (Populus tremula x Populus...
BOWLER, C, SLOOTEN, L, VANDENBRANDEN, S, DERYCKE, R, BOTTERMAN, J, SYBESMA, C, ...
In plants, environmental adversity often leads to the formation of highly reactive oxygen radicals. Since resistance to such conditions may be correlated with the activity of enzymes involved in...
Shaul, O, Mironov, V, Burssens, S, Van Montagu, M, Inze, D
Cyclins are cell cycle regulators whose proteins oscillate dramatically during the cell cycle. Cyclin steady-state mRNA levels also fluctuate, and there are indications that both their rate of...
Effects of Iron Excess on Nicotiana plumbaginifolia Plants (Implications to Oxidative Stress).
Kampfenkel, K., Van Montagu, M., Inze, D.
Fe excess is believed to generate oxidative stress. To contribute to the understanding of Fe metabolism, Fe excess was induced in Nicotiana plumbaginifolia grown in hydroponic culture upon root...
Slooten, L., Capiau, K., Van Camp, W., Van Montagu, M., Sybesma, C., Inze, D.
Two varieties of tobacco (Nicotiana tabacum var PBD6 and var SR1) were used to generate transgenic lines overexpressing Mn-superoxide dismutase (MnSOD) in the chloroplasts. The overexpressed MnSOD...
Baucher, M., Chabbert, B., Pilate, G., Van Doorsselaere, J., Tollier, M. T., Petit-Conil, M., ...
Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in the biosynthesis of the lignin precursors, the monolignols. We have down-regulated CAD in transgenic poplar (Populus tremula X Populus...
Willekens, H., Van Camp, W., Van Montagu, M., Inze, D., Langebartels, C., Sandermann, H.
We have studied the expression of antioxidant genes in response to near ambient conditions of O3, SO2, and ultraviolet B (UV-B) in Nicotiana plumbaginifolia L. The genes analyzed encode four...
Shaul, O, Mironov, V, Burssens, S, Van Montagu, M, Inze, D
Cyclins are cell cycle regulators whose proteins oscillate dramatically during the cell cycle. Cyclin steady-state mRNA levels also fluctuate, and there are indications that both their rate of...
Effects of Iron Excess on Nicotiana plumbaginifolia Plants (Implications to Oxidative Stress).
Kampfenkel, K., Van Montagu, M., Inze, D.
Fe excess is believed to generate oxidative stress. To contribute to the understanding of Fe metabolism, Fe excess was induced in Nicotiana plumbaginifolia grown in hydroponic culture upon root...
Slooten, L., Capiau, K., Van Camp, W., Van Montagu, M., Sybesma, C., Inze, D.
Two varieties of tobacco (Nicotiana tabacum var PBD6 and var SR1) were used to generate transgenic lines overexpressing Mn-superoxide dismutase (MnSOD) in the chloroplasts. The overexpressed MnSOD...
Baucher, M., Chabbert, B., Pilate, G., Van Doorsselaere, J., Tollier, M. T., Petit-Conil, M., ...
Cinnamyl alcohol dehydrogenase (CAD) catalyzes the last step in the biosynthesis of the lignin precursors, the monolignols. We have down-regulated CAD in transgenic poplar (Populus tremula X Populus...
Willekens, H., Van Camp, W., Van Montagu, M., Inze, D., Langebartels, C., Sandermann, H.
We have studied the expression of antioxidant genes in response to near ambient conditions of O3, SO2, and ultraviolet B (UV-B) in Nicotiana plumbaginifolia L. The genes analyzed encode four...