DNA-based vaccine protects against zoonotic schistosomiasis in water buffalo (2008)
Da'Dara, A. A, Li, Y. S, Xiong, T, Zhou, J, Williams, Gail M., McManus, D. P, ...
Echinococcosis in Ningxia Hui Autonomous Region, northwest China (2008)
Yang, Yu R., Craig, P. S, Sun, T., Vuitton, D. A., Giraudoux, P., Jones, Malcolm K., ...
Cystic echinococcosis (CE), caused by Echinococcus granulosus, and alveolar echinococcosis (AE), due to E. multilocularis, are endemic to large areas of north and northwest China. Here we review...
Ellis, M. K., Raso, G., Li, Y. S., Rong, Z., Chen, H. G., McManus, D. P.
Human helminthiases are common in China, especially in rural areas where sanitation conditions are poor. Co- and multiple infections with helminths are strikingly frequent. A cross-sectional...
Yang, Y. R., Craig, P. S., Ito, A., Vuitton, D. A., Giraudoux, P., Sun, T., ...
We correlated ultrasound (US) imaging classifications for human alveolar echinococcosis (AE) and cystic echinococcosis (CE) with serology (ELISA and immunoblotting (IB) incorporating native and...
Tracking the fate of iron in early development of human blood flukes (2007)
Jones, M. K., McManus, D. P., Sivadorai, P., Glanfield, A., Moertel, L., Belli, S.I., ...
Iron (Fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. One role for Fe in some invertebrates is in stabilization of...
Balen, J., Zhao, Z.Y., Williams, G.M., McManus, D.P., Raso, G., Utzinger, J., ...
Objective To determine the prevalence and intensity of Schistosoma japonicum infection and associated morbidity, and to estimate the infected human and buffalo populations in the Dongting Lake...
Ellis, M. K., Raso, G., Li, Y. S., Rong, Z., Chen, H. G., McManus, D. P.
Human helminthiases are common in China, especially in rural areas where sanitation conditions are poor. Co- and multiple infections with helminths are strikingly frequent. A cross-sectional...
Raso, G., Vounatsou, P., McManus, D. P., N'Goran, E. K., Utzinger, J.
Models that accurately estimate the age-specific infection prevalence of Schistosoma mansoni can be useful for schistosomiasis control programmes, particularly with regard to whether mass drug...
Gray, D.J., Williams, G.M., Li, Y., Chen, H., Li, R.S., Forsyth, S.J., ...
We describe the design and report baseline results of a cluster-randomized intervention to determine the importance of bovines for Schistosoma japonicum transmission in southern China. The study...
Yang, Y. R., Craig, P. S., Ito, A., Vuitton, D. A., Giraudoux, P., Sun, T., ...
We correlated ultrasound (US) imaging classifications for human alveolar echinococcosis (AE) and cystic echinococcosis (CE) with serology (ELISA and immunoblotting (IB) incorporating native and...
Tracking the fate of iron in early development of human blood flukes (2007)
Jones, M. K., McManus, D. P., Sivadorai, P., Glanfield, A., Moertel, L., Belli, S.I., ...
Iron (Fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. One role for Fe in some invertebrates is in stabilization of...
Gray, D. J., Williams, G. M., Li, Y. S., Chen, H. G., Li, R. S., Forsyth, S. J., ...
We describe the design and report baseline results of a cluster-randomized intervention to determine the importance of bovines for Schistosoma japonicum transmission in southern China. The study...
Zhang, D. M., Pan, W. Q., Qian, L., Duke, M., Shen, L. H., McManus, D. P.
Schistosoma japonicum paramyosin, a 97 kDa myofibrillar protein, is a recognized vaccine candidate against schistosomiasis. To improve its expression and to identify protective epitopic regions on...
Zhang, D. M., Pan, W. Q., Qian, L., Duke, M., Shen, L. H., McManus, D. P.
Schistosoma japonicum paramyosin, a 97 kDa myofibrillar protein, is a recognized vaccine candidate against schistosomiasis. To improve its expression and to identify protective epitopic regions on...
Gan, X. X., Shen, L. Y., Wang, Y. Q., Ding, J. Z., Shen, H. Y., Zeng, X. P., ...
A polyhistidine-tagged recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein (SVLBP) from adult Schistosoma japonicum was expressed in Escherichia coli. The...
Smyth, D. J., Glanfield, A., McManus, D. P., Hacker, E., Blair, D., Anderson, G. J., ...
We describe two homologues of the mammalian divalent metal transporter (DMT1) for Schistosoma mansoni, a pathogenic intravascular parasite of humans. Schistosomes have a high nutritional and...
Yang, Y. R., Williams, G. M., Craig, P. S., Sun, T., Yang, S. K., Cheng, L., ...
A comprehensive study of human echinococcosis (caused by Echinococcus granulosus or E. multilocularis), including assessment of hospital records, community surveys and patient follow-up, was...
Ellis, M. K., Li, Y. S., Rong, Z., Chen, H. G., McManus, D. P.
Despite the success of extensive control measures that have been implemented in China for over 50 years, the number of individuals infected with Schistosoma japonicum remains high in the existing...
Molecular discrimination of taeniid cestodes (2006)
DNA approaches are now being used routinely for accurate identification of Echinococcus and Taenia species, subspecies and strains, and in molecular epidemiological surveys of...
Molecular Study of Echinococcus in West-Central China (2005)
Yang, Y. R., Rosenzvit, M. C., Zhang, L. H., Zhang, J. Z., McManus, D. P.
West-central China is an important endemic focus of both alveolar and cystic echinococcosis where several species of intermediate host are commonly infected with Echinococcus granulosus and E....
Yang, Y. R., Vuitton, D. A., Jones, M. K., Craig, P. S., McManus, D. P.
An unusual female case, with alveolar echinococcosis (AE) disseminated from the primary hepatic lesion to the brain by metastasis formation, was retrospectively identified during a community survey...
Pearson, M. S., McManus, D. P., Smyth, D. J., Lewis, F. A., Loukas, A.
Proteins secreted by and anchored on the surfaces of parasites are in intimate contact with host tissues. The transcriptome of infective cercariae of the blood fluke, Schistosoma mansoni, was...
Hydatid disease in the brush-tailed rock-wallaby (2004)
Barnes, T. S., Goldizen, A. W., McManus, D. P., Jenkins, D.J., Coleman, G. T.
Lahmar, S., Debbek, H., Zhang, L. H., McManus, D. P., Souissi, A., Chelly, S., ...
Cystic echinococcosis, caused by Echinococcus grantilosus, is highly endemic in North Africa and the Middle East. This paper examines the abundance and prevalence of infection of E. granulosus in...
Lahmar, S., Debbek, H., Zhang, L. H., McManus, D. P., Souissi, A., Chelly, S., ...
Cystic echinococcosis, caused by Echinococcus grantilosus, is highly endemic in North Africa and the Middle East. This paper examines the abundance and prevalence of infection of E. granulosus in...
Hydatid disease in the brush-tailed rock-wallaby (2004)
Barnes, T. S., Goldizen, A. W., McManus, D. P., Jenkins, D.J., Coleman, G. T.
Hydatid disease in the brush-tailed rock-wallaby (Petrogale penicillata) (2004)
Barnes, T. S., Goldizen, A. W., McManus, D. P., Jenkins, D. J., Coleman, G. T.
Jones, M. K., Gobert, G. N., Zhang, L. H., Sunderland, P., McManus, D. P.
Schistosomes are parasitic blood flukes, responsible for significant human disease in tropical and developing nations. Here we review information on the organization of the cytoskeleton and...
Laser microdissection microscopy in parasitology: microscopes meet thermocyclers (2004)
Jones, M. K., Randall, L. M., McManus, D. P., Engwerda, C. R.
The new methods of laser microdissection microscopy have received wide acceptance in biology and have been applied in a small number of parasitology investigations. Here, the techniques and...
Schistosome transcriptome analysis at the cutting edge (2004)
McManus, D. P., Hu, W., Brindley, P. J., Feng, Z., Han, Z. G.
We live in the era of post-genomics, a term that was, until recently, inappropriate when considering the blood flukes of humans because of the relative lack of knowledge of the schistosome genome....
Li, J., Zhang, W. B., Loukas, A., Lin, R. Y., Ito, A., Zhang, L. H., ...
A full-length cDNA sequence coding for Echinococcus granulosus thioredoxin peroxidase (EgTPx) was isolated from a sheep strain protoscolex cDNA library by immunoscreening using a pool of sera from...
Li, Y. S., Kardorff, R., Richter, J., Sun, K. Y., Zhou, H., McManus, D. P., ...
Hepatosplenic measurements among 550 Chinese subjects, aged 3-59 years from Yueyang city-a nonendemic area for schistosomiasis in Hunan province, China-were performed to define normal ranges of...
Case report: unusual presentation of Fasciolopsis buski in a Viet Namese child (2004)
Le, T. H., De Nguyen, V., Phan, B. U., Blair, D., McManus, D. P.
A Viet Namese child presented with a history of abdominal pain. Shortly afterwards, he vomited eight live trematode flukes that were collected and morphologically identified as Fasciolopsis buski....
Li, Y., Yu, D. B., Li, Y. S., Luo, X. S., Liang, Y. S., Bartley, P. B., ...
To demonstrate the dynamics of specific antibody isotypes against schistosome adult worm (AWA) and soluble egg (SEA) antigens, we evaluated (in 1999-2000) 112 subjects infected with Schistosoma...
McDermott, L., Kennedy, M.W., McManus, D.P., Bradley, J.E., Cooper, A., Storch, J.
Three different classes of small lipid-binding protein (LBP) are found in helminth parasites. Although of similar size, the ABA-1A1 (also designated As-NPA-A1) and Ov-FAR-1 (formerly known as Ov20)...
Le, T.H., Pearson, M.S., Blair, D., Dai, N., Zhang, L.H., McManus, D.P.
Unlike other members of the genus, Echinococcus granulosus is known to exhibit considerable levels of variation in biology, physiology and molecular genetics. Indeed, some of the taxa regarded as...
Williams, G. M., Sleigh, A. C., Li, Y. S., Feng, Z., Davis, G. M., Chen, H. G., ...
We present the first mathematical model on the transmission dynamics of Schistosoma japonicum. The work extends Barbour's classic model of schistosome transmission. It allows for the mammalian host...
McManus, D. P., Wong, J.Y.M., Zhou, J., Cai, C., Zeng, Q., Smyth, D., ...
Li, Y., Yu, D. B., Li, Y. S., Luo, X. S., Liang, Y. S., Bartley, P. B., ...
To demonstrate the dynamics of specific antibody isotypes against schistosome adult worm (AWA) and soluble egg (SEA) antigens, we evaluated (in 1999-2000) 112 subjects infected with Schistosoma...
Tashani, O. A., Zhang, L. H., Boufana, B., Jegi, A., McManus, D. P.
The incidence of surgically confirmed cystic echinococcosis in eastern Libya was estimated to be at least 4.2 cases/100,000, with significantly more female cases than male. The prevalences of...
Revisiting the question of limited genetic variation within Schistosoma japonicum (2002)
Le, T. H., Blair, D., McManus, D. P.
Recent electrophoretic data have indicated that Schistosoma japonicum in mainland China may be a species complex, with the existence of a cryptic species being predicted from the analysis of...
Towards a taxonomic revision of the genus Echinococcus (2002)
Thompson, R. C. A., McManus, D. P.
Echinococcus remains a significant public health problem worldwide and, in several regions, the aetiological agents of cystic hydatid disease/echinococcosis are extending their range. The taxonomy of...
Mitochondrial genomes of parasitic flatworms (2002)
Le, T. H., Blair, D., McManus, D. P.
Complete or near-complete mitochondrial genomes are now available for 11 species or strains of parasitic flatworms belonging to the Trematoda and the Cestoda. The organization of these genomes is not...
McManus, D. P., Zhang, L. H., Castrodale, L. J., Le, T. H., Pearson, M., Blair, D.
Distinct Echinococcus granulosus life cycle patterns have been described in North America: domestic and sylvatic. Gene sequences of the sylvatic E. granulosus indicate that it represents a separate...
Cellular responses to Schistosoma japonicum cathepsin D aspartic protease (2002)
Verity, C. K., McManus, D. P., Brindley, P. J.
Lymphocyte proliferation and cytokine production were measured in groups of mice vaccinated (but not subsequently challenge infected) with recombinant forms of Schistosoma japonicum cathepsin D...
Laha, T., Brindley, P. J., Verity, C. K., McManus, D. P., Loukas, A.
A newly described non-long terminal repeat (non-LTR) retrotransposon element was isolated from the genome of the Oriental schistosome, Schistosoma japonicum. At least 1000 partial copies of the...
Le, T. H., Pearson, M. S., Blair, D., Dai, N., Zhang, L. H., McManus, D. P.
Unlike other members of the genus, Echinococcus granulosus is known to exhibit considerable levels of variation in biology, physiology and molecular genetics. Indeed, some of the taxa regarded as...
Laha, T., Brindley, P. J., Smout, M. J., Verity, C. K., McManus, D. P., Loukas, A.
A new RTE-like, non-long terminal repeat retrotransposon, termed SjR2, from the human blood fluke, Schistosoma japonicum, is described. SjR2 is similar to3.9 kb in length and is constituted of a...
Acosta, L. P., Aligui, G. D. L., Tiu, W. U., McManus, D. P., Olveda, R. M.
This study describes the categorical classification of 155 individuals living in an endemic village in Macanip, Leyte, Philippines as 'resistant' or 'susceptible' to Schistosoma japonicum infection...
McManus, D. P., Ross, A. G. P., Williams, G. M., Sleigh, A. C., Wiest, P., Erlich, H., ...
To identify possible associations between host genetic factors and the onset of liver fibrosis following Schistosoma japonicum infection, the major histocompatibility class II alleles of 84...
Ross, A. G. P., Sleigh, A. C., Li, Y. S., Davis, G. M., Williams, G. M., Jiang, Z., ...
Schistosomiasis japonica is a serious communicable disease and a major disease risk for more than 30 million people living in the tropical and subtropical zones of China. Infection remains a major...
Li, Y. S., Sleigh, A. C., Ross, A. G. P., Li, Y., Zhang, X. Y., Williams, G. M., ...
Antibody isotypic responses (IgE, IgA, IgG1, IgG2, IgG3 and IgG4) to Schistosoma japonicum antigens-adult worm (AWA), soluble egg (SEA) and the recombinant proteins TEG (22.6-kDa tegumental antigen,...
Ross, A. G. P., Li, Y., Williams, G. M., Jiang, Z., McManus, D. P.
Human and animal infection rates with the Oriental schistosome have steadily declined in China over the last half-century, but the Three Gorges Dam may reverse this decline by creating new, or...
Proteolysis of human hemoglobin by schistosome cathepsin D (2001)
Brindley, P. J., Kalinna, B. H., Wong, J. Y. M., Bogitsh, B. J., King, L. T., Smyth, D. J., ...
Schistosomes feed on human blood. They employ proteases to degrade hemoglobin from ingested erythrocytes, using the residues released for amino acid metabolism. However, the identity and the role of...
Jones, M. K., Yang, W., McManus, D. P.
Calponins are proteins present in vertebrate smooth musculature where they occur in association with thin myofilaments. Calponins are not present in vertebrate or invertebrate striated muscles. The...
Receptor for Fc on the surfaces of schistosomes (2001)
Loukas, A., Jones, M. K., King, L. T., Brindley, P. J., McManus, D. P.
Schistosoma mansoni masks its surface with adsorbed host proteins including erythrocyte antigens, immunoglobulins, major histocompatibility complex class I, and beta (2)-microglobulin (beta (2)m),...
Yu, D. B., Li, Y., Sleigh, A. C., Yu, X. L., Li, Y. S., Wei, W. Y., ...
This study conducted in 1999/2000 was designed to evaluate the efficacy of praziquantel against Schistosoma japonicum in an area with repeated chemotherapy (Area A) compared with a newly identified...
Le, T. H., Humair, P. F., Blair, D., Agatsuma, T., Littlewood, D. T. J., McManus, D. P.
Complete sequences were obtained for the coding portions of the mitochondrial (mt) genomes of Schistosoma mansoni (NMRI strain, Puerto Rico; 14415 bp), S. japonicum (Anhui strain, China; 14085 bp)...
Verity, C. K., Loukas, A., McManus, D. P., Brindley, P. J.
Recombinant cathepsin D aspartic protease of Schistosoma japonicum cleaved human IgG in vitro in a time and dose-dependent manner. Optimal cleavage was seen at pH 3.6-4.5; modest cleavage remained at...
Vaccine efficacy of recombinant cathepsin D aspartic protease from Schistosoma japonicum (2001)
Verity, C. K., McManus, D. P., Brindley, P. J.
Mice were vaccinated with recombinant Schistosoma japonicum cathepsin D aspartic protease, expressed in both insect cells and bacteria, in order to evaluate the vaccine efficacy of the schistosome...
Laha, T., Loukas, A., Verity, C. K., McManus, D. P., Brindley, P. J.
We characterized the consensus sequence and structure of a long terminal repeat (LTR) retrotransposon from the genome of the human blood fluke, Schistosoma japonicum, and have earned this element,...
Le, T. H., Blair, D., McManus, D. P.
The complete nucleotide sequence of the mitochondrial (mt) DNA molecule of the liverfluke, Fasciola hepatica (phylum Platyhelminthes, class Trematoda, family Fasciolidae), was determined, It...
Le, T.H., Blair, D., Agatsuma, T., Humair, P. F., Campbell, N. J. H., Iwagami, M., ...
No Abstract
Li, Y., Sleigh, A., Ross, A. G., Li, Y.S., Williams, G. M., Tanner, M., ...
Li, Y., Sleigh, A. C., Williams, G. M., Ross, A. G. P., Tanner, M., Li, Y. S., ...
We used activity diaries and snail detection to relate water contact and Schistosoma japonicum infection among a cohort of 178 residents on two islands in the Dongting Lake, China. Water exposure to...
Is there immunity to schistosoma japonicum (2000)
Ross, A. G., Sleigh, A., Li, Y., Williams, G. M., Algui, G. D. L., McManus, D. P.
The Oriental schistosome, Schistosoma japonicum, unlike the other two major schistosomes that infect humans (S. mansoni and S. haematobium), is a zoonotic species. The transmission dynamics and the...
Li, Y., Sleigh, A., Ross, A. G., Williams, G. M., Tanner, M., McManus, D. P.
Dongting Lake, covering a very large surface water area of 2691km2, is located in Hunan Province in the southern part of the People's Republic of China. It is the second-largest freshwater lake in...
Li, Y. S., Sleigh, A. C., Ross, A. G. P., Li, Y., Williams, G. M., Tanner, M., ...
We studied a community cohort of 193 individuals exposed to endemic Schistosoma japonicum infection in the Dongting Lake region of China to assess subclinical morbidity and the 2-year benefit of...
McManus, D. P., Ross, A. G., Sleigh, A., Williams, G. M., Yang, W., Li, Y. S., ...
Interleukin-10 (IL-10) cytokine production was assessed using peripheral blood mononuclear cells (PBMC) from 67 individuals living in an area endemic for schistosomiasis japonica in China (Dongting...
Characterisation of a family of Schistosoma japonicum proteins related to dynein light chains (1999)
Yang, W., Jones, M. K., Hughes-Stamm, S. R., Fan, J. J., McManus, D. P.
Ultrastructural localization of Echinococcus granulosus laminin-binding protein (1999)
Jones, M. K., Zhang, L. H., Gould, R. J., McManus, D. P.
Murine antibodies, raised against a purified recombinant 30 kDa laminin-binding protein from Echinococcus granulosus, were used to investigate the tissue distribution of the native protein in...
Molecular characterization of a calponin-like protein from Schistosoma japonicum (1999)
Yang, W., Zheng, Y. Z., Jones, M. K., McManus, D. P.
The gene for a Schistosoma japonicum (Philippine strain origin) (Sjp) calponin-like protein has been cloned and characterised. The clone, designated P14, was isolated from a Sjp adult worm lambda ZAP...
Li, Yue Sheng, Ross, Allen G. P., Sleigh, A. C., Li, Yi, Waine, G. J., Williams, G. J., ...
Antibody isotype responses to adult worm antigen (AWA) of Schistosoma japonicum and two recombinant proteins (paramyosin (PMY) and a 22 kDa tegumental membrane-associated antigen (TEG)) were analyzed...
Li, Y. S., Sleigh, A. C., Ross, A. G. P., Li, Y., Williams, G. M., Forsyth, S. J., ...
In 1996, 250 people living in the Dongting-Lake region of China were selected for a 2-year study. All had been or were infected with Schistosoma japonicum. All were treated with praziquantel,...
He, Y. K., Li, Y., Liu, S. X., Luo, X. S., Yu, X. L., Zhang, X. Y., ...
The levels of antibodies which react with the cercarial antigens (CA), schistosomulum-stage antigens (SSA), adult-worm antigens (AWA) and soluble egg antigens (SEA) of Schistosoma japonicum were...
Identification of novel 70-kDa heat shock protein-encoding cDNAs from schistosoma japonicum (1999)
A diverse range of organisms respond to a variety of chemical, physiological and temperature-associated stresses by a rapid and transient increase in the synthesis of heat shock proteins. We...
Mitochondrial genes of schistosoma mansoni (1999)
Blair, D., Le, T. H., Despres, L., McManus, D. P.
Two clones, totalling 8068 bp and spanning over half of the coding region of the mitochondrial genome of Schistosoma, mansoni, have been sequenced. Complete sequences are presented of the large and...
McManus, D. P., Ross, A. G., Sleigh, A., Williams, G. M., Yang, W., Li, Y. S., ...
Interleukin-10 (IL-10) cytokine production was assessed using peripheral blood mononuclear cells (PBMC) from 67 individuals living in an area endemic for schistosomiasis japonica in China (Dongting...
Li, Y. S., Ross, A. G. P., Yu, D. B., Li, Y., Williams, G. M., McManus, D. P.
We examined three Chinese villages (one farming village and two fishing villages) in an area highly endemic for schistosomiasis japonica in order to study the prevalence, intensity of infection and...
Ross, A. G. P., Li, Y. S., Williams, G. M., Li, Y., Sleigh, A. S., Zhang, X., ...
In 1995-1996 we conducted an epidemiological survey in two communities (1656 individuals) on Qingshan island, Hunan province P.R. China, in order to determine the efficacy of current control...
Emerging and reemerging helminthiases and the public health of China.
Hotez, P. J., Zheng, F., Long-qi, X., Ming-gang, C., Shu-hua, X., Shu-xian, L., ...
Despite great strides in their control throughout the People's Republic of China, helminth infections remain an important public health problem. The Institute of Parasitic Diseases of the Chinese...