Cardenas, Paula P., Carrasco, Begoña, Sanchez, Humberto, Deikus, Gintaras, Bechhofer, David H, Alonso, Juan C
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN...
Yao, Shiyi, Blaustein, Joshua B., Bechhofer, David H.
Small cytoplasmic RNA (scRNA) of Bacillus subtilis is the RNA component of the signal recognition particle. scRNA is transcribed as a 354-nt precursor, which is processed to the mature 271-nt scRNA....
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Bechhofer, David H., Figurski, David H.
From our earlier work, we know that the korA gene of broad host range plasmid RK2 is located within the 50.4'-56.4' region. By additional sub-cloning of this region, we have mapped korA to the...
Protection against 3′-to-5′ RNA Decay in Bacillus subtilis
Farr, Glen A., Oussenko, Irina A., Bechhofer, David H.
A 320-nucleotide RNA with several characteristic features was expressed in Bacillus subtilis to study RNA processing. The RNA consisted of a 5′-proximal sequence from bacteriophage SP82 containing...
Bechhofer, David H., Stasinopoulos, Stan J.
A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant...
Decay of ermC mRNA in a Polynucleotide Phosphorylase Mutant of Bacillus subtilis
Bechhofer, David H., Wang, Wei
ermC mRNA decay was examined in a mutant of Bacillus subtilis that has a deleted pnpA gene (coding for polynucleotide phosphorylase). 5′-proximal RNA fragments less than 400 nucleotides in length...
Oussenko, Irina A., Bechhofer, David H.
Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major...
Tetracycline Induces Stabilization of mRNA in Bacillus subtilis
The tet(L) gene of Bacillus subtilis confers low-level tetracycline (Tc) resistance. Previous work examining the >20-fold-inducible expression of tet(L) by Tc demonstrated a 12-fold translational...
Jin, Jie, Guffanti, Arthur A., Bechhofer, David H., Krulwich, Terry A.
The Tet(L) protein encoded in the Bacillus subtilis chromosome and the closely related Tet(K) protein from Staphylococcus aureus plasmids are multifunctional antiporters that have three cytoplasmic...
Bacillus subtilis YhaM, a Member of a New Family of 3′-to-5′ Exonucleases in Gram-Positive Bacteria
Oussenko, Irina A., Sanchez, Roberto, Bechhofer, David H.
A strain of Bacillus subtilis lacking two 3′-to-5′ exoribonucleases, polynucleotide phosphorylase (PNPase) and RNase R, was used to purify another 3′-to-5′ exoribonuclease, which is encoded...
A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
Hicks, David B., Wang, ZhenXiong, Wei, Yi, Kent, Rebecca, Guffanti, Arthur A., Banciu, Horia, ...
The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes...
Effect of Translational Signals on mRNA Decay in Bacillus subtilis
Sharp, Josh S., Bechhofer, David H.
A 254-nucleotide model mRNA, designated ΔermC mRNA, was used to study the effects of translational signals and ribosome transit on mRNA decay in Bacillus subtilis. ΔermC mRNA features a strong...
Recycling of a regulatory protein by degradation of the RNA to which it binds
Deikus, Gintaras, Babitzke, Paul, Bechhofer, David H.
When Bacillus subtilis is grown in the presence of excess tryptophan, transcription of the trp operon is regulated by binding of tryptophan-activated TRAP to trp leader RNA, which promotes...
Oussenko, Irina A., Sanchez, Roberto, Bechhofer, David H.
In a continuing effort to identify ribonucleases that may be involved in mRNA decay in Bacillus subtilis, fractionation of a protein extract from a triple-mutant strain that was missing three...
Participation of 3′-to-5′ Exoribonucleases in the Turnover of Bacillus subtilis mRNA
Oussenko, Irina A., Abe, Teppei, Ujiie, Hiromi, Muto, Akira, Bechhofer, David H.
Four 3′-to-5′ exoribonucleases have been identified in Bacillus subtilis: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Mutant strains were constructed that were lacking...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Campos-Guillén, Juan, Bralley, Patricia, Jones, George H., Bechhofer, David H., Olmedo-Alvarez, Gabriela
Polyadenylation plays a role in decay of some bacterial mRNAs, as well as in the quality control of stable RNA. In Escherichia coli, poly(A) polymerase I (PAP I) is the main polyadenylating enzyme,...
Protection against 3′-to-5′ RNA Decay in Bacillus subtilis
Farr, Glen A., Oussenko, Irina A., Bechhofer, David H.
A 320-nucleotide RNA with several characteristic features was expressed in Bacillus subtilis to study RNA processing. The RNA consisted of a 5′-proximal sequence from bacteriophage SP82 containing...
Bechhofer, David H., Stasinopoulos, Stan J.
A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant...
Decay of ermC mRNA in a Polynucleotide Phosphorylase Mutant of Bacillus subtilis
Bechhofer, David H., Wang, Wei
ermC mRNA decay was examined in a mutant of Bacillus subtilis that has a deleted pnpA gene (coding for polynucleotide phosphorylase). 5′-proximal RNA fragments less than 400 nucleotides in length...
Oussenko, Irina A., Bechhofer, David H.
Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major...
Tetracycline Induces Stabilization of mRNA in Bacillus subtilis
The tet(L) gene of Bacillus subtilis confers low-level tetracycline (Tc) resistance. Previous work examining the >20-fold-inducible expression of tet(L) by Tc demonstrated a 12-fold translational...
Jin, Jie, Guffanti, Arthur A., Bechhofer, David H., Krulwich, Terry A.
The Tet(L) protein encoded in the Bacillus subtilis chromosome and the closely related Tet(K) protein from Staphylococcus aureus plasmids are multifunctional antiporters that have three cytoplasmic...
Bacillus subtilis YhaM, a Member of a New Family of 3′-to-5′ Exonucleases in Gram-Positive Bacteria
Oussenko, Irina A., Sanchez, Roberto, Bechhofer, David H.
A strain of Bacillus subtilis lacking two 3′-to-5′ exoribonucleases, polynucleotide phosphorylase (PNPase) and RNase R, was used to purify another 3′-to-5′ exoribonuclease, which is encoded...
A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
Hicks, David B., Wang, ZhenXiong, Wei, Yi, Kent, Rebecca, Guffanti, Arthur A., Banciu, Horia, ...
The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes...
Effect of Translational Signals on mRNA Decay in Bacillus subtilis
Sharp, Josh S., Bechhofer, David H.
A 254-nucleotide model mRNA, designated ΔermC mRNA, was used to study the effects of translational signals and ribosome transit on mRNA decay in Bacillus subtilis. ΔermC mRNA features a strong...
Recycling of a regulatory protein by degradation of the RNA to which it binds
Deikus, Gintaras, Babitzke, Paul, Bechhofer, David H.
When Bacillus subtilis is grown in the presence of excess tryptophan, transcription of the trp operon is regulated by binding of tryptophan-activated TRAP to trp leader RNA, which promotes...
Oussenko, Irina A., Sanchez, Roberto, Bechhofer, David H.
In a continuing effort to identify ribonucleases that may be involved in mRNA decay in Bacillus subtilis, fractionation of a protein extract from a triple-mutant strain that was missing three...
Participation of 3′-to-5′ Exoribonucleases in the Turnover of Bacillus subtilis mRNA
Oussenko, Irina A., Abe, Teppei, Ujiie, Hiromi, Muto, Akira, Bechhofer, David H.
Four 3′-to-5′ exoribonucleases have been identified in Bacillus subtilis: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Mutant strains were constructed that were lacking...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Campos-Guillén, Juan, Bralley, Patricia, Jones, George H., Bechhofer, David H., Olmedo-Alvarez, Gabriela
Polyadenylation plays a role in decay of some bacterial mRNAs, as well as in the quality control of stable RNA. In Escherichia coli, poly(A) polymerase I (PAP I) is the main polyadenylating enzyme,...
Adaptive Gene Expression in Bacillus subtilis Strains Deleted for tetL
Wei, Yi, Deikus, Gintaras, Powers, Benjamin, Shelden, Victor, Krulwich, Terry A., Bechhofer, David H.
The growth properties of a new panel of Bacillus subtilis tetL deletion strains and of a derivative set of strains in which tetL is restored to the chromosome support earlier indications that...
Yao, Shiyi, Blaustein, Joshua B., Bechhofer, David H.
Small cytoplasmic RNA (scRNA) of Bacillus subtilis is the RNA component of the signal recognition particle. scRNA is transcribed as a 354-nt precursor, which is processed to the mature 271-nt scRNA....
Deikus, Gintaras, Condon, Ciarán, Bechhofer, David H.
The 140-nucleotide trp leader RNA, which is formed by transcription termination under conditions of high intracellular tryptophan, was used to study RNA turnover in Bacillus subtilis. We showed in...
Bacillus subtilis polynucleotide phosphorylase 3′-to-5′ DNase activity is involved in DNA repair
Cardenas, Paula P., Carrasco, Begoña, Sanchez, Humberto, Deikus, Gintaras, Bechhofer, David H, Alonso, Juan C
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN...