David H. Figurski

ORFcurator: molecular curation of genes and gene clusters in prokaryotic organisms (2004)

Rosenfeld, Jeffrey A., Sarkar, Indra N., Planet, Paul J., Figurski, David H., DeSalle, Rob

Summary: The ability to detect clusters of functionally related genes in multiple microbial genomes has enormous potential for enhancing studies on gene function and microbial evolution. The...

ORFcurator: molecular curation of genes and gene clusters in prokaryotic organisms (2004)

Rosenfeld, Jeffrey A., Sarkar, Indra N., Planet, Paul J., Figurski, David H., DeSalle, Rob

The ability to detect clusters of functionally related genes in multiple microbial genomes has enormous potential for enhancing studies on gene function and microbial evolution. The staggering amount...

ORFcurator: molecular curation of genes and gene clusters in prokaryotic organisms (2004)

Rosenfeld, Jeffrey A., Sarkar, Indra N., Planet, Paul J., Figurski, David H., DeSalle, Rob

The ability to detect clusters of functionally related genes in multiple microbial genomes has enormous potential for enhancing studies on gene function and microbial evolution. The staggering amount...

Nonspecific Adherence by Actinobacillus actinomycetemcomitans Requires Genes Widespread in Bacteria and Archaea (2000)

Scott C. Kachlany, Paul J. Planet, Mrinal K. Bhattacharjee, Evyenia Kollia, Rob Desalle, Daniel H. Fine, ...

The gram-negative coccobacillus, Actinobacillus actinomycetemcomitans, is the putative agent for localized juvenile periodontitis, a particularly destructive form of periodontal disease in...

Map location and nucleotide sequence of korA, a key regulatory gene of promiscuous ptasmid RK2 (1983)

Bechhofer, David H., Figurski, David H.

From our earlier work, we know that the korA gene of broad host range plasmid RK2 is located within the 50.4'-56.4' region. By additional sub-cloning of this region, we have mapped korA to the...

Phylogeny of genes for secretion NTPases: Identification of the widespread tadA subfamily and development of a diagnostic key for gene classification

Planet, Paul J., Kachlany, Scott C., DeSalle, Rob, Figurski, David H.

Macromolecular transport systems in bacteria currently are classified by function and sequence comparisons into five basic types. In this classification system, type II and type IV secretion systems...

Incompatibility Protein IncC and Global Regulator KorB Interact in Active Partition of Promiscuous Plasmid RK2

Rosche, Thomas M., Siddique, Azeem, Larsen, Michelle H., Figurski, David H.

Replication of the broad-host-range, IncPα plasmid RK2 requires two plasmid loci: trfA, the replication initiator gene, and oriV, the origin of replication. While these determinants are sufficient...

Nonspecific Adherence by Actinobacillus actinomycetemcomitans Requires Genes Widespread in Bacteria and Archaea

Kachlany, Scott C., Planet, Paul J., Bhattacharjee, Mrinal K., Kollia, Evyenia, DeSalle, Rob, Fine, Daniel H., ...

The gram-negative coccobacillus, Actinobacillus actinomycetemcomitans, is the putative agent for localized juvenile periodontitis, a particularly destructive form of periodontal disease in...

Secretion of RTX Leukotoxin by Actinobacillus actinomycetemcomitans

Kachlany, Scott C., Fine, Daniel H., Figurski, David H.

Actinobacillus actinomycetemcomitans, the etiologic agent for localized juvenile periodontitis and certain other human infections, such as endocarditis, expresses a leukotoxin that acts on...

Nonspecific Adherence and Fibril Biogenesis by Actinobacillus actinomycetemcomitans: TadA Protein Is an ATPase

Bhattacharjee, Mrinal K., Kachlany, Scott C., Fine, Daniel H., Figurski, David H.

Cells of Actinobacillus actinomycetemcomitans, a gram-negative pathogen responsible for an aggressive form of juvenile periodontitis, form tenaciously adherent biofilms on solid surfaces. The...

Direct Selection of IS903 Transposon Insertions by Use of a Broad-Host-Range Vector: Isolation of Catalase-Deficient Mutants of Actinobacillus actinomycetemcomitans

Thomson, Valeri J., Bhattacharjee, Mrinal K., Fine, Daniel H., Derbyshire, Keith M., Figurski, David H.

Transposon mutagenesis in bacteria generally requires efficient delivery of a transposon suicide vector to allow the selection of relatively infrequent transposition events. We have developed an...

The Active Partition Gene incC of IncP Plasmids Is Required for Stable Maintenance in a Broad Range of Hosts

Siddique, Azeem, Figurski, David H.

Plasmids of incompatibility group P (IncP) are capable of replication and stable inheritance in a wide variety of gram-negative bacteria. Three determinants of IncP plasmids are components of an...

Tight-adherence genes of Actinobacillus actinomycetemcomitans are required for virulence in a rat model

Schreiner, Helen C., Sinatra, Kathy, Kaplan, Jeffrey B., Furgang, David, Kachlany, Scott C., Planet, Paul J., ...

Actinobacillus actinomycetemcomitans is a Gram-negative coccobacillus that has been associated with localized aggressive periodontitis and infections of the heart, brain, and urinary tract. Wild-type...

Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans

Figurski, David H., Helinski, Donald R.

pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases),...

Phylogeny of genes for secretion NTPases: Identification of the widespread tadA subfamily and development of a diagnostic key for gene classification

Planet, Paul J., Kachlany, Scott C., DeSalle, Rob, Figurski, David H.

Macromolecular transport systems in bacteria currently are classified by function and sequence comparisons into five basic types. In this classification system, type II and type IV secretion systems...

Incompatibility Protein IncC and Global Regulator KorB Interact in Active Partition of Promiscuous Plasmid RK2

Rosche, Thomas M., Siddique, Azeem, Larsen, Michelle H., Figurski, David H.

Replication of the broad-host-range, IncPα plasmid RK2 requires two plasmid loci: trfA, the replication initiator gene, and oriV, the origin of replication. While these determinants are sufficient...

Nonspecific Adherence by Actinobacillus actinomycetemcomitans Requires Genes Widespread in Bacteria and Archaea

Kachlany, Scott C., Planet, Paul J., Bhattacharjee, Mrinal K., Kollia, Evyenia, DeSalle, Rob, Fine, Daniel H., ...

The gram-negative coccobacillus, Actinobacillus actinomycetemcomitans, is the putative agent for localized juvenile periodontitis, a particularly destructive form of periodontal disease in...

Secretion of RTX Leukotoxin by Actinobacillus actinomycetemcomitans

Kachlany, Scott C., Fine, Daniel H., Figurski, David H.

Actinobacillus actinomycetemcomitans, the etiologic agent for localized juvenile periodontitis and certain other human infections, such as endocarditis, expresses a leukotoxin that acts on...

Nonspecific Adherence and Fibril Biogenesis by Actinobacillus actinomycetemcomitans: TadA Protein Is an ATPase

Bhattacharjee, Mrinal K., Kachlany, Scott C., Fine, Daniel H., Figurski, David H.

Cells of Actinobacillus actinomycetemcomitans, a gram-negative pathogen responsible for an aggressive form of juvenile periodontitis, form tenaciously adherent biofilms on solid surfaces. The...

Direct Selection of IS903 Transposon Insertions by Use of a Broad-Host-Range Vector: Isolation of Catalase-Deficient Mutants of Actinobacillus actinomycetemcomitans

Thomson, Valeri J., Bhattacharjee, Mrinal K., Fine, Daniel H., Derbyshire, Keith M., Figurski, David H.

Transposon mutagenesis in bacteria generally requires efficient delivery of a transposon suicide vector to allow the selection of relatively infrequent transposition events. We have developed an...

The Active Partition Gene incC of IncP Plasmids Is Required for Stable Maintenance in a Broad Range of Hosts

Siddique, Azeem, Figurski, David H.

Plasmids of incompatibility group P (IncP) are capable of replication and stable inheritance in a wide variety of gram-negative bacteria. Three determinants of IncP plasmids are components of an...

Tight-adherence genes of Actinobacillus actinomycetemcomitans are required for virulence in a rat model

Schreiner, Helen C., Sinatra, Kathy, Kaplan, Jeffrey B., Furgang, David, Kachlany, Scott C., Planet, Paul J., ...

Actinobacillus actinomycetemcomitans is a Gram-negative coccobacillus that has been associated with localized aggressive periodontitis and infections of the heart, brain, and urinary tract. Wild-type...

Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans

Figurski, David H., Helinski, Donald R.

pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases),...

The TadV Protein of Actinobacillus actinomycetemcomitans Is a Novel Aspartic Acid Prepilin Peptidase Required for Maturation of the Flp1 Pilin and TadE and TadF Pseudopilins†

Tomich, Mladen, Fine, Daniel H., Figurski, David H.

The tad locus of Actinobacillus actinomycetemcomitans encodes genes for the biogenesis of Flp pili, which allow the bacterium to adhere tenaciously to surfaces and form strong biofilms. Although tad...

Interaction between Leukotoxin and Cu,Zn Superoxide Dismutase in Aggregatibacter actinomycetemcomitans▿

Balashova, Nataliya V., Park, Diane H., Patel, Jigna K., Figurski, David H., Kachlany, Scott C.

Aggregatibacter (Actinobacillus) actinomycetemcomitans is a gram-negative oral pathogen that is the etiologic agent of localized aggressive periodontitis and systemic infections. A....

Outer Membrane Components of the Tad (Tight Adherence) Secreton of Aggregatibacter actinomycetemcomitans▿ †

Clock, Sarah A., Planet, Paul J., Perez, Brenda A., Figurski, David H.

Prokaryotic secretion relies on proteins that are widely conserved, including NTPases and secretins, and on proteins that are system specific. The Tad secretion system in Aggregatibacter...

Transcriptional Regulation of the tad Locus in Aggregatibacter actinomycetemcomitans: a Termination Cascade▿

Kram, Karin E., Hovel-Miner, Galadriel A., Tomich, Mladen, Figurski, David H.

The tad (tight adherence) locus of Aggregatibacter actinomycetemcomitans includes genes for the biogenesis of Flp pili, which are necessary for bacterial adhesion to surfaces, biofilm formation, and...

Bacterial Conjugation in the Cytoplasm of Mouse Cells▿

Lim, Yin Mei, Bhattacharjee, Mrinal K., Figurski, David H., Schon, Eric A.

Intracellular pathogenic organisms such as salmonellae and shigellae are able to evade the effects of many antibiotics because the drugs are not able to penetrate the plasma membrane. In addition,...