David I. Yule

Ultrastructure and cell-cell coupling of cardiac myocytes differentiating in embryonic stem cell cultures (1997)

Westfall, Margaret V., Pasyk, Krystyna A., Yule, David I., Samuelson, Linda C., Metzger, Joseph M.

Differentiation cultures of embryonic stem (ES) cells can be a useful in vitro system for understanding cardiac myocyte development. However, cell morphometry, sarcomere development, and functional...

CCK antagonists reveal that CCK-8 and JMV-180 interact with different sites on the rat pancreatic acinar cell CCKA receptor (1994)

Yule, David I., Williams, John A.

The ability of CCKA antagonists to inhibit full and partial CCK agonists of the rat pancreatic acinar cell CCKA receptor has been studied. When isolated rat pancreatic acini were superfused with...

Endothelin increases [Ca2+]i in rat pancreatic acinar cells by intracellular release but fails to increase amylase secretion (1992)

Yule, David I., Wagner, Andreas C. C., Williams, John A.

In individual fura-2 loaded cells of rat pancreatic acini endothelin-1 (ET-1) (10-50 nM) induced sustained oscillations in [Ca2+]i. At higher concentrations a larger, but transient increase in...

Mastoparan induces oscillations of cytosolic Ca2+ in rat pancreatic acinar cells (1991)

Yule, David I., Williams, John A.

Microfluorimetry of fura-2 was used to monitor [Ca2+]i in single cells stimulated with the G-protein activating agent mastoparan. Mastoparan induced the generation of [Ca2+]i oscillations, which in...

Agonist activation of arachidonate-regulated Ca2+-selective (ARC) channels in murine parotid and pancreatic acinar cells

Mignen, Olivier, Thompson, Jill L, Yule, David I, Shuttleworth, Trevor J

ARC channels (arachidonate-regulated Ca2+-selective channels) are a novel type of highly Ca2+-selective channel that are specifically activated by low concentrations of agonist-induced arachidonic...

Agonist activation of arachidonate-regulated Ca2+-selective (ARC) channels in murine parotid and pancreatic acinar cells

Mignen, Olivier, Thompson, Jill L, Yule, David I, Shuttleworth, Trevor J

ARC channels (arachidonate-regulated Ca2+-selective channels) are a novel type of highly Ca2+-selective channel that are specifically activated by low concentrations of agonist-induced arachidonic...

Calcium Wave Propagation in Pancreatic Acinar Cells: Functional Interaction of Inositol 1,4,5-Trisphosphate Receptors, Ryanodine Receptors, and Mitochondria

Straub, Stephen V., Giovannucci, David R., Yule, David I.

In pancreatic acinar cells, inositol 1,4,5-trisphosphate (InsP3)–dependent cytosolic calcium ([Ca2+]i) increases resulting from agonist stimulation are initiated in an apical “trigger zone,”...

Cytosolic Ca2+ and Ca2+-activated Cl− current dynamics: insights from two functionally distinct mouse exocrine cells

Giovannucci, David R, Bruce, Jason I. E, Straub, Stephen V, Arreola, Jorge, Sneyd, James, Shuttleworth, Trevor J, ...

The dynamics of Ca2+ release and Ca2+-activated Cl− currents in two related, but functionally distinct exocrine cells, were studied to gain insight into how the molecular specialization of Ca2+...

Agonist-dependent Phosphorylation of the Inositol 1,4,5-Trisphosphate Receptor : A Possible Mechanism for Agonist-specific Calcium Oscillations in Pancreatic Acinar Cells

LeBeau, Andrew P., Yule, David I., Groblewski, Guy E., Sneyd, James

The properties of inositol 1,4,5-trisphosphate (IP3)-dependent intracellular calcium oscillations in pancreatic acinar cells depend crucially on the agonist used to stimulate them. Acetylcholine or...

ATP Modulation of Ca2+ Release by Type-2 and Type-3 Inositol (1, 4, 5)-Triphosphate Receptors: DIFFERING ATP SENSITIVITIES AND MOLECULAR DETERMINANTS OF ACTION*

Betzenhauser, Matthew J., Wagner, Larry E., Iwai, Miwako, Michikawa, Takayuki, Mikoshiba, Katsuhiko, Yule, David I.

ATP enhances Ca2+ release from inositol (1,4,5)-trisphosphate receptors (InsP3R). However, the three isoforms of InsP3R are reported to respond to ATP with differing sensitivities. Ca2+ release...

Regulation of single inositol 1,4,5-trisphosphate receptor channel activity by protein kinase A phosphorylation

Wagner II, Larry E, Joseph, Suresh K, Yule, David I

Phosphorylation of inositol 1,4,5-trisphosphate receptors (InsP3R) by PKA represents an important, common route for regulation of Ca2+ release. Following phosphorylation of the S2 splice variant of...

Visualizing form and function in organotypic slices of the adult mouse parotid gland

Warner, Jennifer D., Peters, Christian G., Saunders, Rudel, Won, Jong Hak, Betzenhauser, Matthew J., Gunning, William T., ...

An organotypic slice preparation of the adult mouse parotid salivary gland amenable to a variety of optical assessments of fluid and protein secretion dynamics is described. The semi-intact...

The Type 2 Inositol (1,4,5)-Trisphosphate (InsP3) Receptor Determines the Sensitivity of InsP3-induced Ca2+ Release to ATP in Pancreatic Acinar Cells*

Park, Hyung Seo, Betzenhauser, Matthew J., Won, Jong Hak, Chen, Ju, Yule, David I.

Calcium release through inositol (1,4,5)-trisphosphate receptors (InsP3R) is the primary signal driving digestive enzyme and fluid secretion from pancreatic acinar cells. The type 2 (InsP3R2) and...