The NH2 terminus of RCK1 domain regulates Ca2+-dependent BK(Ca) channel gating. (2005)
Krishnamoorthy, Gayathri, Shi, Jingyi, Sept, David, Cui, Jianmin
Large conductance, voltage- and Ca2+-activated K+ (BK(Ca)) channels regulate blood vessel tone, synaptic transmission, and hearing owing to dual activation by membrane depolarization and...
Dinitroanilines Bind �-Tubulin to Disrupt (2003)
Naomi S. Morrissette, Arpita Mitra, David Sept, L. David Sibley
Protozoan parasites are remarkably sensitive to dinitroanilines such as oryzalin, which disrupt plant but not animal microtubules. To explore the basis of dinitroaniline action, we isolated 49...
The physical basis of microtubule structure and stability (2003)
Sept, David, Baker, Nathan A., McCammon, J. Andrew
Microtubules are cylindrical polymers found in every eukaryotic cell. They have a unique helical structure that has implications at both the cellular level, in terms of the functions they perform,...
Electrostatics of Nanosystems: Application to microtubules and the ribosome (2001)
Nathan A. Baker, David Sept, Simpson Joseph, Michael J. Holst, J. Andrew Mccammon
Evaluation of the electrostatic properties of biomolecules has become a standard practice in molecular biophysics. Foremost among the models used to elucidate the electrostatic potential is the...
Nathan A. Baker, David Sept, Michael J. Holst, J. Andrew Mccammon
Using new methods for the parallel solution of elliptic partial di#erential equations, the teraflops computing power of massively parallel computers can be leveraged to perform electrostatic...
Electrostatics of nanosystems: Application to microtubules and the ribosome
Baker, Nathan A., Sept, David, Joseph, Simpson, Holst, Michael J., McCammon, J. Andrew
Evaluation of the electrostatic properties of biomolecules has become a standard practice in molecular biophysics. Foremost among the models used to elucidate the electrostatic potential is the...
Sahoo, Nivedita, Beatty, Wandy, Heuser, John, Sept, David, Sibley, L. David
Toxoplasma is a protozoan parasite in the phylum Apicomplexa, which contains a number of medically important parasites that rely on a highly unusual form of motility termed gliding to actively...
Dinitroanilines Bind α-Tubulin to Disrupt Microtubules
Morrissette, Naomi S., Mitra, Arpita, Sept, David, Sibley, L. David
Protozoan parasites are remarkably sensitive to dinitroanilines such as oryzalin, which disrupt plant but not animal microtubules. To explore the basis of dinitroaniline action, we isolated 49...
Clark, Michael G., Teply, Joseph, Haarer, Brian K., Viggiano, Susan C., Sept, David, Amberg, David C.
Actin interacting protein 1 (Aip1p) and cofilin cooperate to disassemble actin filaments in vitro and are thought to promote rapid turnover of actin networks in vivo. The precise method by which...
Electrostatics of nanosystems: Application to microtubules and the ribosome
Baker, Nathan A., Sept, David, Joseph, Simpson, Holst, Michael J., McCammon, J. Andrew
Evaluation of the electrostatic properties of biomolecules has become a standard practice in molecular biophysics. Foremost among the models used to elucidate the electrostatic potential is the...
Dinitroanilines Bind α-Tubulin to Disrupt Microtubules
Morrissette, Naomi S., Mitra, Arpita, Sept, David, Sibley, L. David
Protozoan parasites are remarkably sensitive to dinitroanilines such as oryzalin, which disrupt plant but not animal microtubules. To explore the basis of dinitroaniline action, we isolated 49...
Sahoo, Nivedita, Beatty, Wandy, Heuser, John, Sept, David, Sibley, L. David
Toxoplasma is a protozoan parasite in the phylum Apicomplexa, which contains a number of medically important parasites that rely on a highly unusual form of motility termed gliding to actively...
Clark, Michael G., Teply, Joseph, Haarer, Brian K., Viggiano, Susan C., Sept, David, Amberg, David C.
Actin interacting protein 1 (Aip1p) and cofilin cooperate to disassemble actin filaments in vitro and are thought to promote rapid turnover of actin networks in vivo. The precise method by which...
Myo1c Binds Phosphoinositides through a Putative Pleckstrin Homology Domain
Hokanson, David E., Laakso, Joseph M., Lin, Tianming, Sept, David, Ostap, E. Michael
Myo1c is a member of the myosin superfamily that binds phosphatidylinositol-4,5-bisphosphate (PIP2), links the actin cytoskeleton to cellular membranes and plays roles in mechano-signal transduction...
Energetics and Dynamics of Constrained Actin Filament Bundling
Yang, Le, Sept, David, Carlsson, A. E.
The formation of filopodia-like bundles from a dendritic actin network has been observed to occur in vitro as a result of branching induced by Arp2/3 complex. We study both the energetics and...
Mutations in α-Tubulin Confer Dinitroaniline Resistance at a Cost to Microtubule Function
Ma, Christopher, Li, Catherine, Ganesan, Lakshmi, Oak, Jean, Tsai, Susan, Sept, David, ...
Protozoan microtubules are sensitive to disruption by dinitroanilines, compounds that kill intracellular Toxoplasma gondii parasites without affecting microtubules in vertebrate host cells. We...
The NH2 Terminus of RCK1 Domain Regulates Ca2+-dependent BKCa Channel Gating
Krishnamoorthy, Gayathri, Shi, Jingyi, Sept, David, Cui, Jianmin
Large conductance, voltage- and Ca2+-activated K+ (BKCa) channels regulate blood vessel tone, synaptic transmission, and hearing owing to dual activation by membrane depolarization and intracellular...
The physical basis of microtubule structure and stability
Sept, David, Baker, Nathan A., McCammon, J. Andrew
Microtubules are cylindrical polymers found in every eukaryotic cell. They have a unique helical structure that has implications at both the cellular level, in terms of the functions they perform,...
Nucleotide Effects on the Structure and Dynamics of Actin
Zheng, Xiange, Diraviyam, Karthikeyan, Sept, David
Adenosine 5′-triphosphate or ATP is the primary energy source within the cell, releasing its energy via hydrolysis into adenosine 5′-diphosphate or ADP. Actin is an important ATPase involved in...
Silva, Jonathan R., Pan, Hua, Wu, Dick, Nekouzadeh, Ali, Decker, Keith F., Cui, Jianmin, ...
Ion-channel function is determined by its gating movement. Yet, molecular dynamics and electrophysiological simulations were never combined to link molecular structure to function. We performed...
Neurodegeneration mutations in dynactin impair dynein-dependent nuclear migration
Moore, Jeffrey K., Sept, David, Cooper, John A.
Neurodegenerative disease in humans and mice can be caused by mutations affecting the microtubule motor dynein or its biochemical regulator, dynactin, a multiprotein complex required for dynein...
Taxol is a commonly used antitumor agent that hyperstabilizes microtubules and prevents cell division. The interaction of Taxol with tubulin and the microtubule has been studied through a wide array...