Leippe, M., Tannich, E., Nickel, R., Pattus, F., Horstmann, R. D., ...
A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was...
Deletion mutagenesis in M13 by polymerase chain reaction using universal sequencing primers (1990)
Tannich, E., Lingelbach, K., Tümmler, M.
A simple procedure is described for the efficient deletion of large DNA sequences. The method involves a combination of oligonucleotide-directed mutagenesis in bacteriophage M13 and amplification of...
Braun, T., Buschhausen-Denker, G., Tannich, E.
A segment of the 5'-flanking region of the chicken cardiac myosin light-chain gene extending from nucleotide-64 to the RNA start site is sufficient to allow muscle-specific transcription. We...
Braun, T., Bober, E., Tannich, E., Buschhausen-Denker, G.
We have isolated the cDNA encoding a novel human myogenic factor, Myf-5, by weak cross-hydrization to the mouse MyoD1 probe. Nucleotide sequence analysis and the identification of corresponding gene...
Genomic DNA differences between pathogenic and nonpathogenic Entamoeba histolytica (1989)
Horstmann, R.D., Knobloch, J., Tannich, E.
DNA libraries were constructed from pathogenic (HM-1:IMSS) and nonpathogenic (SAW 1734) isolates of Entamoeba histolytica. A cDNA clone (cEH-P1) specific for pathogenic amoebae was identified by...
Arnold, H.H., Tannich, E., Paterson, B.M.
Transcriptional regulation of the chicken cardiac myosin light chain 2 (MLC2-A) gene was investigated in chicken primary myoblast and fibroblast cultures transfected with vector constructs containing...
Hamann, L, Nickel, R, Tannich, E
To provide tools for functional molecular genetics of the protozoan parasite Entamoeba histolytica, we investigated the use of the prokaryotic neomycin phosphotransferase (NEO) gene as a selectable...
Hybrid plasmids were constructed and used for successful transfection and transient expression of the chloramphenicol acetyltransferase (CAT) gene in the protozoan parasite Entamoeba histolytica....
Meyer, C G, Gallin, M, Erttmann, K D, Brattig, N, Schnittger, L, Gelhaus, A, ...
Human infections with the tissue nematode Onchocerca volvulus result in a variety of clinical conditions that possibly include protective immunity. In a West African area hyperendemic for human...
Primary structure of the 170-kDa surface lectin of pathogenic Entamoeba histolytica.
Tannich, E, Ebert, F, Horstmann, R D
The adherence of Entamoeba histolytica to colonic mucins and to host cells appears to be predominantly mediated by a 170-kDa surface lectin of the amoebae. By using an antiserum to the purified...
We previously reported the identification of homologous cDNA clones derived from a pathogenic isolate and a nonpathogenic isolate of Entamoeba histolytica, which had been designated cEh-P1 and...
Lotter, H, Mannweiler, E, Schreiber, M, Tannich, E
A recombinantly expressed protein, recEh-P1, representing part of an immunodominant surface antigen of pathogenic Entamoeba histolytica, was used for serodiagnosis of invasive amebiasis. Expression...
Genomic DNA differences between pathogenic and nonpathogenic Entamoeba histolytica.
Tannich, E, Horstmann, R D, Knobloch, J, Arnold, H H
cDNA libraries were constructed from pathogenic (HM-1:IMSS) and nonpathogenic (SAW 1734) isolates of Entamoeba histolytica. A cDNA clone (cEH-P1) specific for pathogenic amoebae was identified by...
Arnold, H H, Tannich, E, Paterson, B M
Transcriptional regulation of the chicken cardiac myosin light chain 2 (MLC2-A) gene was investigated in chicken primary myoblast and fibroblast cultures transfected with vector constructs containing...
Braun, T, Tannich, E, Buschhausen-Denker, G, Arnold, H H
A segment of the 5'-flanking region of the chicken cardiac myosin light-chain gene extending from nucleotide -64 to the RNA start site is sufficient to allow muscle-specific transcription. In this...
Braun, T, Buschhausen-Denker, G, Bober, E, Tannich, E, Arnold, H H
We have isolated the cDNA encoding a novel human myogenic factor, Myf-5, by weak cross-hydridization to the mouse MyoD1 probe. Nucleotide sequence analysis and the identification of the corresponding...
Primary and secondary structure of the pore-forming peptide of pathogenic Entamoeba histolytica.
Leippe, M, Tannich, E, Nickel, R, Pattus, F, Horstmann, R D, ...
A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was...
A bifunctional 95 kDa polypeptide (EhADH2) harbouring acetaldehyde dehydrogenase and alcohol dehydrogenase activities was purified to homogeneity from trophozoite extracts of the protozoan parasite...
Bruchhaus, I, Jacobs, T, Denart, M, Tannich, E
By using oligonucleotide primers derived from regions highly conserved in prokaryotic and eukaryotic phosphofructokinase sequences, a genomic DNA fragment was amplified and used to isolate cDNA and...
Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica.
Bruchhaus, I, Richter, S, Tannich, E
The 29 kDa protein of Entamoeba histolytica (Eh29), as well as a truncated variant of this protein, which lacks a cysteine-rich N-terminal region of 40 amino acid residues (Eh29mut), were...
Bruchhaus, I, Richter, S, Tannich, E
The gene encoding a putative NADPH:flavin oxidoreductase of the protozoan parasite Entamoeba histolytica (Eh34) was recombinantly expressed in Escherichia coli. The purified recombinant protein...
Hamann, L, Nickel, R, Tannich, E
To provide tools for functional molecular genetics of the protozoan parasite Entamoeba histolytica, we investigated the use of the prokaryotic neomycin phosphotransferase (NEO) gene as a selectable...
Hybrid plasmids were constructed and used for successful transfection and transient expression of the chloramphenicol acetyltransferase (CAT) gene in the protozoan parasite Entamoeba histolytica....
Meyer, C G, Gallin, M, Erttmann, K D, Brattig, N, Schnittger, L, Gelhaus, A, ...
Human infections with the tissue nematode Onchocerca volvulus result in a variety of clinical conditions that possibly include protective immunity. In a West African area hyperendemic for human...
Primary structure of the 170-kDa surface lectin of pathogenic Entamoeba histolytica.
Tannich, E, Ebert, F, Horstmann, R D
The adherence of Entamoeba histolytica to colonic mucins and to host cells appears to be predominantly mediated by a 170-kDa surface lectin of the amoebae. By using an antiserum to the purified...
We previously reported the identification of homologous cDNA clones derived from a pathogenic isolate and a nonpathogenic isolate of Entamoeba histolytica, which had been designated cEh-P1 and...
Lotter, H, Mannweiler, E, Schreiber, M, Tannich, E
A recombinantly expressed protein, recEh-P1, representing part of an immunodominant surface antigen of pathogenic Entamoeba histolytica, was used for serodiagnosis of invasive amebiasis. Expression...
Genomic DNA differences between pathogenic and nonpathogenic Entamoeba histolytica.
Tannich, E, Horstmann, R D, Knobloch, J, Arnold, H H
cDNA libraries were constructed from pathogenic (HM-1:IMSS) and nonpathogenic (SAW 1734) isolates of Entamoeba histolytica. A cDNA clone (cEH-P1) specific for pathogenic amoebae was identified by...
Arnold, H H, Tannich, E, Paterson, B M
Transcriptional regulation of the chicken cardiac myosin light chain 2 (MLC2-A) gene was investigated in chicken primary myoblast and fibroblast cultures transfected with vector constructs containing...
Braun, T, Tannich, E, Buschhausen-Denker, G, Arnold, H H
A segment of the 5'-flanking region of the chicken cardiac myosin light-chain gene extending from nucleotide -64 to the RNA start site is sufficient to allow muscle-specific transcription. In this...
Braun, T, Buschhausen-Denker, G, Bober, E, Tannich, E, Arnold, H H
We have isolated the cDNA encoding a novel human myogenic factor, Myf-5, by weak cross-hydridization to the mouse MyoD1 probe. Nucleotide sequence analysis and the identification of the corresponding...
Primary and secondary structure of the pore-forming peptide of pathogenic Entamoeba histolytica.
Leippe, M, Tannich, E, Nickel, R, Pattus, F, Horstmann, R D, ...
A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was...
A bifunctional 95 kDa polypeptide (EhADH2) harbouring acetaldehyde dehydrogenase and alcohol dehydrogenase activities was purified to homogeneity from trophozoite extracts of the protozoan parasite...
Bruchhaus, I, Jacobs, T, Denart, M, Tannich, E
By using oligonucleotide primers derived from regions highly conserved in prokaryotic and eukaryotic phosphofructokinase sequences, a genomic DNA fragment was amplified and used to isolate cDNA and...
Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica.
Bruchhaus, I, Richter, S, Tannich, E
The 29 kDa protein of Entamoeba histolytica (Eh29), as well as a truncated variant of this protein, which lacks a cysteine-rich N-terminal region of 40 amino acid residues (Eh29mut), were...
Bruchhaus, I, Richter, S, Tannich, E
The gene encoding a putative NADPH:flavin oxidoreductase of the protozoan parasite Entamoeba histolytica (Eh34) was recombinantly expressed in Escherichia coli. The purified recombinant protein...