TonB-dependent transporters and their occurrence in cyanobacteria (2009)
Mirus, Oliver, Strauss, Sascha, Nicolaisen, Kerstin, Von Haeseler, Arndt, Schleiff, Enrico
Abstract Background Different iron transport systems evolved in Gram-negative bacteria during evolution. Most of the transport systems depend on outer membrane localized TonB-dependent transporters...
Bionda, Tihana, Koenig, Patrick, Oreb, Mislav, Tews, Ivo, Schleiff, Enrico
The properties of membrane-embedded GTPases are investigated to understand translocation of preprotein across the outer envelope of chloroplasts. The homo- and heterodimerization events of the...
Wunder, Tobias, Martin, Roman, Löffelhardt, Wolfgang, Schleiff, Enrico, Steiner, Jürgen M
Abstract Background Today it is widely accepted that plastids are of cyanobacterial origin. During their evolutionary integration into the metabolic and regulatory networks of the host cell the...
Suncana Moslavac, Kerstin Nicolaisen, Oliver Mirus, Fadi Al Dehni, Rafael Pernil, ...
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms heterocysts in a semiregular pattern when it is grown on N 2 as the sole nitrogen source. The transition from vegetative cells to...
Running title: TPRs in preprotein translocation (2007)
Thomas Schlegel, Oliver Mirus, Arndt Von Haeseler, Enrico Schleiff, Enrico Schleiff
The tetratricopeptide repeats of receptors involved in protein
Dual targeting of the tRNA nucleotidyltransferase in plants: not just the signal (2007)
Schmidt Von Braun, Serena, Sabetti, Antonino, Hanic-Joyce, Pamela J., Gu, Jun, Schleiff, Enrico, Joyce, Paul B. M.
Enzymes involved in tRNA maturation are essential for cytosolic, mitochondrial, and plastid protein synthesis and are therefore localized to these different compartments of the cell. Interestingly,...
The Tetratricopeptide Repeats of Receptors Involved in Protein Translocation across Membranes (2007)
Schlegel, Thomas, Mirus, Oliver, Von Haeseler, Arndt, Schleiff, Enrico
Transport of polypeptides across membranes is a general and essential process in every cell. This process is utilized by molecular machines composed of soluble and membrane-inserted proteins. At...
Prediction of β-barrel membrane proteins by searching for restricted domains (2005)
Mirus, Oliver, Schleiff, Enrico
Abstract Background The identification of β-barrel membrane proteins out of a genomic/proteomic background is one of the rapidly developing fields in bioinformatics. Our main goal is the prediction...
Schleiff, Enrico, Eichacker, Lutz Andreas, Eckart, Kerstin, Becker, Thomas, Mirus, Oliver, Stahl, Tanja, ...
In the postgenomic era, the transformation of genetic information into biochemical meaning is required. We have analyzed the proteome of the chloroplast outer envelope membrane by an in silico and a...
Protein import into mitochondria : a study on hTom20 (1998)
Tom20 is a major receptor for protein translocation located on the outer mitochondrial membrane. This protein is a member of a multicomponent complex and is found to stimulate import of all classes...
Protein import into mitochondria : a study on hTom20 (1998)
Tom20 is a major receptor for protein translocation located on the outer mitochondrial membrane. This protein is a member of a multicomponent complex and is found to stimulate import of all classes...
Toc34 is a preprotein receptor regulated by GTP and phosphorylation
Sveshnikova, Natalia, Soll, Jürgen, Schleiff, Enrico
Most proteins present in chloroplasts are synthesized in the cytosol and are posttranslationally translocated into the organelle. A multicomponent translocation machinery located in both the outer...
Lipid Composition of Outer Leaflet of Chloroplast Outer Envelope Determines Topology of OEP7
Schleiff, Enrico, Tien, Roselynn, Salomon, Michael, Soll, Jürgen
OEP7, a 6.7-kDa outer envelope protein of spinach chloroplasts inserts into the outer envelope of the organelle independent of a classical cleavable targeting signal. The insertion of OEP7 was...
A GTP-driven motor moves proteins across the outer envelope of chloroplasts
Schleiff, Enrico, Jelic, Marko, Soll, Jürgen
The translocation of proteins across cellular membranes is a key mechanistic problem for every cell. The preprotein translocon at the chloroplast outer envelope is responsible for precursor protein...
Toc12, a Novel Subunit of the Intermembrane Space Preprotein Translocon of Chloroplasts
Becker, Thomas, Hritz, Jozef, Vogel, Markus, Caliebe, Alexander, Bukau, Bernd, Soll, Jürgen, ...
Translocation of proteins across membranes is essential for the biogenesis of each cell and is achieved by proteinaceous complexes. We analyzed the translocation complex of the intermembrane space...
A Toc75-like protein import channel is abundant in chloroplasts
Eckart, Kerstin, Eichacker, Lutz, Sohrt, Karen, Schleiff, Enrico, Heins, Lisa, Soll, Jürgen
Chloroplasts import post-translationally most of their constituent polypeptides via two distinct translocon units located in the outer and inner envelope. The protein import channel of the translocon...
Preprotein recognition by the Toc complex
Becker, Thomas, Jelic, Marko, Vojta, Aleksandar, Radunz, Alfons, Soll, Jürgen, Schleiff, Enrico
The Toc core complex consists of the pore-forming Toc75 and the GTPases Toc159 and Toc34. We confirm that the receptor form of Toc159 is integrated into the membrane. The association of Toc34 to...
Membrane protein insertion: mixing eukaryotic and prokaryotic concepts
Schleiff, Enrico, Soll, Jürgen
Proteins are translocated across or inserted into membranes by machines that are composed of soluble and membrane-anchored subunits. The molecular action of these machines and their evolutionary...
Toc34 is a preprotein receptor regulated by GTP and phosphorylation
Sveshnikova, Natalia, Soll, Jürgen, Schleiff, Enrico
Most proteins present in chloroplasts are synthesized in the cytosol and are posttranslationally translocated into the organelle. A multicomponent translocation machinery located in both the outer...
Lipid Composition of Outer Leaflet of Chloroplast Outer Envelope Determines Topology of OEP7
Schleiff, Enrico, Tien, Roselynn, Salomon, Michael, Soll, Jürgen
OEP7, a 6.7-kDa outer envelope protein of spinach chloroplasts inserts into the outer envelope of the organelle independent of a classical cleavable targeting signal. The insertion of OEP7 was...
A GTP-driven motor moves proteins across the outer envelope of chloroplasts
Schleiff, Enrico, Jelic, Marko, Soll, Jürgen
The translocation of proteins across cellular membranes is a key mechanistic problem for every cell. The preprotein translocon at the chloroplast outer envelope is responsible for precursor protein...
Toc12, a Novel Subunit of the Intermembrane Space Preprotein Translocon of Chloroplasts
Becker, Thomas, Hritz, Jozef, Vogel, Markus, Caliebe, Alexander, Bukau, Bernd, Soll, Jürgen, ...
Translocation of proteins across membranes is essential for the biogenesis of each cell and is achieved by proteinaceous complexes. We analyzed the translocation complex of the intermembrane space...
A Toc75-like protein import channel is abundant in chloroplasts
Eckart, Kerstin, Eichacker, Lutz, Sohrt, Karen, Schleiff, Enrico, Heins, Lisa, Soll, Jürgen
Chloroplasts import post-translationally most of their constituent polypeptides via two distinct translocon units located in the outer and inner envelope. The protein import channel of the translocon...
Preprotein recognition by the Toc complex
Becker, Thomas, Jelic, Marko, Vojta, Aleksandar, Radunz, Alfons, Soll, Jürgen, Schleiff, Enrico
The Toc core complex consists of the pore-forming Toc75 and the GTPases Toc159 and Toc34. We confirm that the receptor form of Toc159 is integrated into the membrane. The association of Toc34 to...
Membrane protein insertion: mixing eukaryotic and prokaryotic concepts
Schleiff, Enrico, Soll, Jürgen
Proteins are translocated across or inserted into membranes by machines that are composed of soluble and membrane-anchored subunits. The molecular action of these machines and their evolutionary...
The molecular chaperone Hsp90 delivers precursor proteins to the chloroplast import receptor Toc64
Qbadou, Soumya, Becker, Thomas, Mirus, Oliver, Tews, Ivo, Soll, Jürgen, Schleiff, Enrico
Precursor protein targeting toward organellar surfaces is assisted by different cytosolic chaperones. We demonstrate that the chloroplast protein translocon subunit Toc64 is the docking site for...
Kempa, Stefan, Rozhon, Wilfried, Šamaj, Jozef, Erban, Alexander, Baluška, František, Becker, Thomas, ...
Glycogen synthase kinase 3 (GSK-3) was originally identified as a regulator of glycogen synthesis in mammals. Like starch in plants, glycogen is a polymer of glucose, and serves as an energy and...
A TolC-Like Protein Is Required for Heterocyst Development in Anabaena sp. Strain PCC 7120▿ †
Moslavac, Suncana, Nicolaisen, Kerstin, Mirus, Oliver, Al Dehni, Fadi, Pernil, Rafael, Flores, Enrique, ...
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms heterocysts in a semiregular pattern when it is grown on N2 as the sole nitrogen source. The transition from vegetative cells to...
Schleiff, Enrico, Silvius, John R., Shore, Gordon C.
Insertion of newly synthesized proteins into or across the mitochondrial outer membrane is initiated by import receptors at the surface of the organelle. Typically, this interaction directs the...
Characterization of the translocon of the outer envelope of chloroplasts
Schleiff, Enrico, Soll, Jürgen, Küchler, Michael, Kühlbrandt, Werner, Harrer, Roswitha
The protein translocon of the outer envelope of chloroplasts (Toc) consists of the core subunits Toc159, Toc75, and Toc34. To investigate the molecular structure, the core complex was purified. This...
Prediction of the plant β-barrel proteome: A case study of the chloroplast outer envelope
Schleiff, Enrico, Eichacker, Lutz Andreas, Eckart, Kerstin, Becker, Thomas, Mirus, Oliver, Stahl, Tanja, ...
In the postgenomic era, the transformation of genetic information into biochemical meaning is required. We have analyzed the proteome of the chloroplast outer envelope membrane by an in silico and a...
Phosphorylation regulates the assembly of chloroplast import machinery
Oreb, Mislav, Höfle, Anja, Mirus, Oliver, Schleiff, Enrico
Chloroplast function depends on the translocation of cytosolically synthesized precursor proteins into the organelle. The recognition and transfer of most precursor proteins across the outer membrane...
Nicolaisen, Kerstin, Moslavac, Suncana, Samborski, Anastazia, Valdebenito, Marianne, Hantke, Klaus, Maldener, Iris, ...
Iron uptake in proteobacteria by TonB-dependent outer membrane transporters represents a well-explored subject. In contrast, the same process has been scarcely investigated in cyanobacteria. The...
Moving the green: CHUP1 and chloroplast movement—An obvious relationship?
Schmidt Von Braun, Serena, Schleiff, Enrico
Chloroplast movement as a response of plants to light variations is presented as an example in each classical textbook, showing that these organelles accumulate in response to low light and avoid...