Evelyne Kohli

Crystallization and Preliminary X-Ray Analysis of Rotavirus Protein VP6

Petitpas, Isabelle, Lepault, Jean, Vachette, Patrice, Charpilienne, Annie, Mathieu, Magali, Kohli, Evelyne, ...

As a first step to gain insight into the structure of the rotavirus virion at atomic resolution, we report here the expression, purification, and crystallization of recombinant rotavirus protein VP6....

Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response

Fromantin, Catherine, Jamot, Béatrice, Cohen, Jean, Piroth, Lionel, Pothier, Pierre, Kohli, Evelyne

We investigated the rotavirus-specific lymphocyte responses induced by intranasal immunization of adult BALB/c mice with rotavirus 2/6 virus-like particles (2/6-VLPs) of the bovine RF strain, by...

Frequencies of Virus-Specific CD4+ and CD8+ T Lymphocytes Secreting Gamma Interferon after Acute Natural Rotavirus Infection in Children and Adults

Jaimes, María C., Rojas, Olga Lucía, González, Ana María, Cajiao, Isabela, Charpilienne, Annie, Pothier, Pierre, ...

Human rotavirus-specific CD4+ and CD8+ T-cell responses in peripheral blood lymphocytes were studied using a flow cytometric assay that detects the intracellular accumulation of cytokines after...

Virus Diversity in a Winter Epidemic of Acute Diarrhea in France

Chikhi-Brachet, Roxane, Bon, Fabienne, Toubiana, Laurent, Pothier, Pierre, Nicolas, Jean-Claude, Flahault, Antoine, ...

In France, an epidemic peak of acute diarrhea is observed each winter. Previous results suggested a viral etiology for these winter epidemics. We investigated the role of enteric viruses in acute...

Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion

Mathieu, Magali, Petitpas, Isabelle, Navaza, Jorge, Lepault, Jean, Kohli, Evelyne, Pothier, Pierre, ...

The structural protein VP6 of rotavirus, an important pathogen responsible for severe gastroenteritis in children, forms the middle layer in the triple-layered viral capsid. Here we present the...

Molecular Characterization of Isolates of Waterborne Cryptosporidium spp. Collected during an Outbreak of Gastroenteritis in South Burgundy, France

Dalle, Frédéric, Roz, Pascale, Dautin, Guillaume, Di-Palma, Marc, Kohli, Evelyne, Sire-Bidault, C., ...

In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs...

Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

Nicollier-Jamot, Béatrice, Pico, Valérie, Pothier, Pierre, Kohli, Evelyne

Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1,078 age-stratified human sera collected...

Virological and Pharmacological Parameters Predicting the Response to Lopinavir-Ritonavir in Heavily Protease Inhibitor-Experienced Patients

Marcelin, Anne-Geneviève, Cohen-Codar, Isabelle, King, Martin S., Colson, Philippe, Guillevic, Emmanuel, Descamps, Diane, ...

The genotypic inhibitory quotient (GIQ) has been proposed as a way to integrate drug exposure and genotypic resistance to protease inhibitors and can be useful to enhance the predictivity of...

Crystallization and Preliminary X-Ray Analysis of Rotavirus Protein VP6

Petitpas, Isabelle, Lepault, Jean, Vachette, Patrice, Charpilienne, Annie, Mathieu, Magali, Kohli, Evelyne, ...

As a first step to gain insight into the structure of the rotavirus virion at atomic resolution, we report here the expression, purification, and crystallization of recombinant rotavirus protein VP6....

Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response

Fromantin, Catherine, Jamot, Béatrice, Cohen, Jean, Piroth, Lionel, Pothier, Pierre, Kohli, Evelyne

We investigated the rotavirus-specific lymphocyte responses induced by intranasal immunization of adult BALB/c mice with rotavirus 2/6 virus-like particles (2/6-VLPs) of the bovine RF strain, by...

Frequencies of Virus-Specific CD4+ and CD8+ T Lymphocytes Secreting Gamma Interferon after Acute Natural Rotavirus Infection in Children and Adults

Jaimes, María C., Rojas, Olga Lucía, González, Ana María, Cajiao, Isabela, Charpilienne, Annie, Pothier, Pierre, ...

Human rotavirus-specific CD4+ and CD8+ T-cell responses in peripheral blood lymphocytes were studied using a flow cytometric assay that detects the intracellular accumulation of cytokines after...

Virus Diversity in a Winter Epidemic of Acute Diarrhea in France

Chikhi-Brachet, Roxane, Bon, Fabienne, Toubiana, Laurent, Pothier, Pierre, Nicolas, Jean-Claude, Flahault, Antoine, ...

In France, an epidemic peak of acute diarrhea is observed each winter. Previous results suggested a viral etiology for these winter epidemics. We investigated the role of enteric viruses in acute...

Atomic structure of the major capsid protein of rotavirus: implications for the architecture of the virion

Mathieu, Magali, Petitpas, Isabelle, Navaza, Jorge, Lepault, Jean, Kohli, Evelyne, Pothier, Pierre, ...

The structural protein VP6 of rotavirus, an important pathogen responsible for severe gastroenteritis in children, forms the middle layer in the triple-layered viral capsid. Here we present the...

Molecular Characterization of Isolates of Waterborne Cryptosporidium spp. Collected during an Outbreak of Gastroenteritis in South Burgundy, France

Dalle, Frédéric, Roz, Pascale, Dautin, Guillaume, Di-Palma, Marc, Kohli, Evelyne, Sire-Bidault, C., ...

In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs...

Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

Nicollier-Jamot, Béatrice, Pico, Valérie, Pothier, Pierre, Kohli, Evelyne

Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1,078 age-stratified human sera collected...

Virological and Pharmacological Parameters Predicting the Response to Lopinavir-Ritonavir in Heavily Protease Inhibitor-Experienced Patients

Marcelin, Anne-Geneviève, Cohen-Codar, Isabelle, King, Martin S., Colson, Philippe, Guillevic, Emmanuel, Descamps, Diane, ...

The genotypic inhibitory quotient (GIQ) has been proposed as a way to integrate drug exposure and genotypic resistance to protease inhibitors and can be useful to enhance the predictivity of...

Detection of Multiple Noroviruses Associated with an International Gastroenteritis Outbreak Linked to Oyster Consumption

Le Guyader, Françoise S., Bon, Fabienne, DeMedici, Dario, Parnaudeau, Sylvain, Bertone, Alessandra, Crudeli, Silvia, ...

An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and...

European Multicenter Evaluation of Commercial Enzyme Immunoassays for Detecting Norovirus Antigen in Fecal Samples▿

Gray, Jim J., Kohli, Evelyne, Ruggeri, Franco M., Vennema, Harry, Sánchez-Fauquier, Alicia, Schreier, Eckart, ...

A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus...