Kaufmann, K., Muiño, J.M., Jauregui, R., Airoldi, C.A., Angenent, G.C.
he molecular mechanisms by which floral homeotic genes act as major developmental switches to specify the identity of floral organs are still largely unknown. Floral homeotic genes encode...
SEPALLATA3: the 'glue' for MADS box transcription factor complex formation (2009)
Immink, G.H., Tonaco, I.A.N., Shchennikova, A., Busscher-Lange, J., ...
Background Plant MADS box proteins play important roles in a plethora of developmental processes. In order to regulate specific sets of target genes, MADS box proteins dimerize and are thought to...
Kaufmann, K., Muiño, J.M., Jauregui, R., Airoldi, C.A., Smaczniak, C., Krajewski, W., ...
The molecular mechanisms by which floral homeotic genes act as major developmental switches to specify the identity of floral organs are still largely unknown. Floral homeotic genes encode...
Jaspers, P., Blomster, T., Brosché, M., Salojärvi, J., Ahlfors, R., Vainonen, J.P., ...
RADICAL-INDUCED CELL DEATH1 (RCD1) is an important regulator of stress and hormonal and developmental responses in Arabidopsis thaliana. Together with its closest homolog, SIMILAR TO RCD-ONE1 (SRO1),...
Dijk, A.D.J., Immink, G.H., Angenent, G.C.
Motivation: Transcription factor interactions are the cornerstone of combinatorial control, which is a crucial aspect of the gene regulatory system. Understanding and predicting transcription factor...
Bemer, M., Wolters-Arts, M., Grossniklaus, U., Angenent, G.C.
MADS box genes in plants consist of MIKC-type and type I genes. While MIKC-type genes have been studied extensively, the functions of type I genes are still poorly understood. Evidence suggests that...
Motivation: Transcription factor interactions are the cornerstone of combinatorial control, which is a crucial aspect of the gene regulatory system. Understanding and predicting transcription factor...
Ciannamea, S., Jensen, C.S., Agerskov, H., Petersen, K., Lenk, I., Didion, T., ...
A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is...
Martens, S., Jonker, H.H., Rosin, F.M.A., Molthoff, J.W., ...
Parthenocarpy, the formation of seedless fruits in the absence of functional fertilization, is a desirable trait for several important crop plants, including tomato (Solanum lycopersicum). Seedless...
Srinivasan, C., Liu, Z., Heidmann, I., Supena, E.D.J., Fukuoka, H., Joosen, R.V.L., ...
Gain-of-function studies have shown that ectopic expression of the BABY BOOM (BBM) AP2/ERF domain transcription factor is sufficient to induce spontaneous somatic embryogenesis in Arabidopsis...
Plant translational genomics: from model species to crops (2007)
Salentijn, E.M.J., Pereira, A.B., Angenent, G.C., Krens, F.A., Smulders, M.J.M., ...
Plant genomic research now faces the ultimate challenge to develop applications in crop plants which implies the translation of gene functions from a model to a crop which is the field of `Plant...
Srinivasan, C., Liu, Z., Heidmann, I., Supena, E.D.J., Fukuoka, H., Joosen, R.V.L., ...
Gain-of-function studies have shown that ectopic expression of the BABY BOOM (BBM) AP2/ERF domain transcription factor is sufficient to induce spontaneous somatic embryogenesis in Arabidopsis...
Tagging of MADS domain proteins for chromatin immunoprecipitation (2007)
Urbanus, S.L., Zuijlen, L., Kaufmann, K., Angenent, G.C.
Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns. To date, knowledge about transcription...
Plant translational genomics: from model species to crops (2007)
Salentijn, E.M.J., Pereira, A.B., Angenent, G.C., Krens, F.A., Smulders, M.J.M., ...
Plant genomic research now faces the ultimate challenge to develop applications in crop plants which implies the translation of gene functions from a model to a crop which is the field of `Plant...
In vivo imaging of MADS-box transcription factor interactions (2006)
Nougalli Tonaco, I.A., Borst, J.W., Angenent, G.C., Immink, R.G.H.
MADS-box transcription factors are major regulators of development in flowering plants. The factors act in a combinatorial manner, either as homo- or heterodimers, and they control floral organ...
Isolation and characterization of an AGAMOUS homologue from cocoa (2006)
Chaidamsari, T., Sugiarit, H., Santoso, D., Angenent, G.C.
We report the cloning of a cDNA from TcAG, an AG (Arabidopsis thaliana MADS-box C-type transcription factor gene AGAMOUS) homologue from cocoa (Theobroma cacao L.). TcAG was in the cocoa flower...
Control of floral meristem determinancy in petunia by MADS box transcription factors (2006)
Ferrario, S.I.T., Shchennikova, A.V., Franken, J., Immink, R.G.H., Angenent, G.C.
The shoot apical meristem (SAM), a small group of undifferentiated dividing cells, is responsible for the continuous growth of plants. Several genes have been identified that control the development...
Characterization of the vernalization response in Lolium perenne by a cDNA microarry approach (2006)
Ciannamea, S., Busscher-Lange, J., Angenent, G.C., Immink, R.G.H.
Many plant species including temperate grasses require vernalization in order to flower. Vernalization is the process of promotion of flowering after exposure to prolonged periods of cold. To...
Characterization of oil palm MADS box genes in relation to the mantled flower abnormality (2006)
Syed Alwee, S., Angenent, G.C., Cheah, S.C., ...
In vitro propagation of oil palm (Elaeis guineensis Jacq.) frequently induces a somaclonal variant called `mantled¿ abnormality, in which the stamens of both male and female flowers are transformed...
Trans meets cis in MADS science (2006)
The interaction between a transcription factor and its binding site at the DNA is an integral part of transcriptional regulatory networks, which is fundamental for an understanding of biological...
A Bsister MADS-box gene involved in ovule and seed development in petunia and Arabidopsis. (2006)
Shchennikova, A.V., Franken, J., Busscher, M., Baskar, R., Grossniklaus, U., ...
MADS-domain transcription factors are essential for proper flower and seed development in angiosperms and their role in determination of floral organ identity can be described by the 'ABC model' of...
Ciannamea, S., Kaufmann, K., Frau, M., Nougalli Tonaco, I.A., Petersen, K., Nielsen, K.K., ...
Regulation of flowering time is best understood in the dicot model species Arabidopsis thaliana. Molecular analyses revealed that genes belonging to the MADS box transcription factor family play...
Use of Petunia to unravel plant meristem functioning (2005)
Angenent, G.C., Stuurman, J., Snowden, K.C., Koes, R.
In the past decade, enormous progress has been made in our understanding of the molecular and genetic control of meristem growth, maintenance and differentiation into plant organs. Several model...
Chalfun Junior, A., Franken, J., Mes, J.J., Marsch-Martinez, N., Pereira, A.B., Angenent, G.C.
The formation and the development of the floral organs require an intercalate expression of organ-specific genes. At the same time, meristem-specific genes are repressed to complete the...
Comprehensive interaction map of the Arabidopsis MADS box transcription factors (2005)
Immink, R.G.H., Kieffer, M, Parenicová, L., Henz, S.R, Weigel, D., ...
Interactions between proteins are essential for their functioning and the biological processes they control. The elucidation of interaction maps based on yeast studies is a first step toward the...
Gomez-Mena, C, Costa, M.M.R, Angenent, G.C., Sablowski, R
Floral organs, whose identity is determined by specific combinations of homeotic genes, originate from a group of undifferentiated cells called the floral meristem. In Arabidopsis, the homeotic gene...
Floral homeotic mutants in lily: double flower and Festiva phenotypes (2005)
Benedito, V.A., Angenent, G.C., Krens, F.A.
Homeotic changes in floral organs of lily (Lilium spp.) are described in this chapter. Usually, lily flowers show similar organs in their first and second whorls called tepals. They constitute the...
Conservation and diversity in flower land (2004)
Ferrario, S.I.T., Immink, R.G.H., Angenent, G.C.
During the past decade, enormous progress has been made in understanding the molecular regulation of flower development. In particular, homeotic genes that determine the identity of the floral organs...
The Petunia ortholog of Arabidopsis SUPERMAN plays a distinct role in floral morphogenesis (2004)
Nakagawa, H., Ferrario, S.I.T., Angenent, G.C., Kobayashi, A., Takatsuji, H.
Arabidopsis (Arabidopsis thaliana) SUPERMAN (SUP) plays a role in establishing a boundary between whorls 3 and 4 of flowers and in ovule development. We characterized a Petunia hybrida (petunia)...
Ferrario, S.I.T., Busscher-Lange, J., Franken, J., Gerts, T., Vandenbussche, M., Angenent, G.C., ...
Several genes belonging to the MADS box transcription factor family have been shown to be involved in the transition from vegetative to reproductive growth. The Petunia hybrida MADS box gene UNSHAVEN...
Shchennikova, A.V., Shulga, O.A., Immink, R., Skryabin, K.G., Angenent, G.C.
Four full-length MADS-box cDNAs from chrysanthemum, designated Chrysanthemum Dendrathema grandiflorum MADS (CDM) 8, CDM41, CDM111, and CDM44, have been isolated and further functionally...
Benedito, V.A., Visser, P.B., Angenent, G.C., Krens, F.A.
Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the...
Lilium longiflorum and molecular floral development: the ABCDE model (2004)
Benedito, V.A., Angenent, G.C., Krens, F.A.
Because lily (Lilium longiflorum Thunb.) is an important cut-flower crop, molecular characterisation of genes that are involved in flower morphology could help breeders to develop novel floral...
Benedito, V.A., Visser, P.B., Angenent, G.C., Krens, F.A.
Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the...
Benedito, V.A., Visser, P.B., Angenent, G.C., Krens, F.A.
The ABC model for floral development was proposed more than 10 years ago and since then many studies have been performed on model species, such as Arabidopsis thaliana, Antirrhinum majus, and many...
Incorporation of 3D plant structures in genetic and physiological models (2004)
Marcelis, L.F.M., Angenent, G.C., Evers, J.B., Struik, P.C., ...
The recently developed virtual plant modelling approach has strongly increased the potential of model applications in crop sciences. Virtual plants are based on a new modelling concept and are...
Bino, R.J., Lommen, A., Hall, R.D., Sumner, L.W., ...
Medicago truncatula is the model plant for molecular and genetic studies of legumes, which are important agricultural protein crops. Furthermore, legumes synthesize several unique (pharmaceutical...
Methods to identify in vivo target genes in Arabidopsis (2004)
Several large families of transcription factors exist in plants, among them the MADS-box gene family, which comprises a little over 100 members. In plants they are involved in e.g. flowering, flower...
Bino, R.J., Lommen, A., Hall, R.D., Sumner, L.W., ...
Medicago truncatula is the model plant for molecular and genetic studies of legumes, which are important agricultural protein crops. Furthermore, legumes synthesize several unique (pharmaceutical...
Benedito, V.A., Visser, P.B., Angenent, G.C., Krens, F.A.
Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the...
Analysis of the petunia MADS-box transcription factor family (2003)
Immink, R.G.H., Ferrario, S.I.T., Busscher-Lange, J., Kooiker, M., Busscher, M., Angenent, G.C.
Transcription factors are key regulators of plant development. One of the major groups of transcription factors is the MADS-box family, of which at least 80 members are encoded in the Arabidopsis...
The MADS Box Gene FBP2 Is Required for SEPALLATA Function in Petunia (2003)
Ferrario, S.I.T., Immink, R.G.H., Shchennikova, A., Busscher-Lange, J., Angenent, G.C.
The ABC model, which was accepted for almost a decade as a paradigm for flower development in angiosperms, has been subjected recently to a significant modification with the introduction of the new...
Parenicova, L., Kieffer, M, Horner, D.S., Favalli, C., Busscher, J., ...
MADS-box transcription factors are key regulators of several plant development processes. Analysis of the complete Arabidopsis genome sequence revealed 107 genes encoding MADS-box proteins, of which...
Vandenbussche, M., Zethof, J., Souer, E., Koes, R., Tornielli, G.B., Pezzotti, M., ...
We have initiated a systematic functional analysis of the MADS box, intervening region, K domain, C domain-type MADS box gene family in petunia. The starting point for this has been a...
Chalfun-Junior, A., Mes, J.J., Mlynarova, L., Aarts, M.G.M., Angenent, G.C.
A powerful system to create gain-of-function mutants in plants is activation tagging using T-DNA based vehicles to introduce transcriptional enhancer sequences. Large Arabidopsis populations of...
Kater, M.M., Franken, J., Inggamer, H., Gretenkort, M., Mollema, C., ...
We have developed a novel strategy for the introduction of durable insect resistance in crops. This strategy was based on intervention in the natural relationship between plants and insects. For many...
Analysis of MADS box protein-protein interactions in living plant cells (2002)
Immink, R.G.H., Gadella Jr., T.W.J., Ferrario, S., Busscher, M., Angenent, G.C.
Over the last decade, the yeast two-hybrid system has become the tool to use for the identification of protein-protein interactions and recently, even complete interactomes were elucidated by this...
Favaro, R., Immink, R.G.H., Feriolo, V., Bernasconi, B., Byzova, M., Angenent, G.C., ...
OsMADS13 is a rice MADS-box gene that is specifically expressed in developing ovules. The amino acid sequence of OsMADS13 shows 74␜imilarity to those of FLORAL BINDING PROTEIN 7 (FBP7) and FBP11,...
Immink, R.G.H., Angenent, G.C.
Proteinprotein interactions are intrinsic to virtually every cellular process. Recent breakthroughs in techniques to study protein-interaction and the availability of fully sequenced plant genomes...
Analysis of MADS box protein-protein interactions in living plant cells (2002)
Immink, R.G.H., Gadella, T.W.J., Ferrario, S.I.T., Busscher, M., Angenent, G.C.
Over the last decade, the yeast two-hybrid system has become the tool to use for the identification of protein-protein interactions and recently, even complete interactomes were elucidated by this...
Immink, R.G.H., Angenent, G.C.
Protein–protein interactions are intrinsic to virtually every cellular process. Recent breakthroughs in techniques to study protein-interaction and the availability of fully sequenced plant genomes...
Favaro, R., Immink, R.G.H., Ferioli, V., Bernasconi, B., Byzova, M., Angenent, G.C., ...
OsMADS13 is a rice MADS-box gene that is specifically expressed in developing ovules. The amino acid sequence of OsMADS13 shows 74␜imilarity to those of FLORAL BINDING PROTEIN 7 (FBP7) and FBP11,...
Analysis of MADS box protein-protein interactions in living plant cells. (2002)
Immink, R.G.H., Gadella, Th.W.J., Ferrario, S., Busscher, M., Angenent, G.C.
Over the last decade, the yeast two-hybrid system has become the tool to use for the identification of protein-protein interactions and recently, even complete interactomes were elucidated by this...
Partial silencing of the NEC1 gene results in early opening of anthers in Petunia hybrida (2001)
Ge, Y.X., Angenent, G.C., Dahlhaus, E., Franken, J., Peters, J., Wullems, G.J., ...
The NEC1 gene, previously isolated from Petunia hybrida, is expressed at high levels in nectaries, and in a very localized fashion in stamens, particularly in the anther stomium cells and the upper...
Sex-determination in the monoecius species cucumber is confined to specific floral whorls (2001)
Kater, M.M., Franken, J., Carney, K.J., Colombo, L., Angenent, G.C.
In unisexual flowers, sex is determined by the selective repression of growth or the abortion of either male or female reproductive organs. The mechanism by which this process is controlled in plants...
Kater, M.M., Franken, J., Angenent, G.C.
Molecular and genetic analyses have shown that the Arabidopsis thaliana gene SUPERMAN (SUP) has at least two functions in Arabidopsis flower development. SUP is necessary to control the correct...
NEC1, a novel gene, highly expressed in nectary tissue of Petunia hybrida (2000)
Ge, Y.X., Angenent, G.C., Wittich, P.E., Peters, J., Franken, J., Busscher, M., ...
NEC1, a novel gene, highly expressed in the nectary tissue of Petunia hybrida (2000)
Ge, X.Y., Angenent, G.C., Wittich, P.E., Peters, J., Franken, J., Busscher, M., ...
To study the molecular regulation of nectary development, we cloned NEC1, a gene predominantly expressed in the nectaries of Petunia hybrida, by using the differential display RTPCR technique. The...
Immink, R.G.H., Hannapel, D.J., Ferrario, S., Busscher, M., Franken, J., Lookeren Campagne, M.M., ...
Immink, R.G.H., Hannapel, D.J., Ferrario, S., Busscher, M., Franken, J., Van Lookeren Campagne, M.M., ...
EMS and transposon mutagenesis for the isolation of apomictic mutants in plants (1998)
Ramulu, K.S., Dijkhuis, P., Pereira, A., Angenent, G.C., Dons, J.J.M.
New molecular genetic strategies: Apomixis improves crops (1997)
Ramulu, K.S., Dijkhuis, R., Pereira, A., Angenent, G.C., Dons, J.J.M.
MADS box genes expressed in developing inflorescences of rice and sorghum (1997)
Greco, R., Stagi, L., Colombo, L., Angenent, G.C., Sari-Gorla, M., Pé, M.E.
EMS mutagenesis and transposon tagging for the induction of apomictic mutants in Arabidopsis (1996)
Ramulu, K.S., Dijkhuis, P., Pereira, A., Angenent, G.C., Dons, J.J.M.
The Petunia MADS box gens FBP11 determines ovule identity. (1995)
Colombo, L., Franken, J., Koetje, E., Dons, H.J.M., Angenent, G.C., ...
Kater, M M, Colombo, L, Franken, J, Busscher, M, Masiero, S, Van Lookeren Campagne, M M, ...
The C function in Arabidopsis, which specifies stamen and carpel identity, is represented by a single gene called AGAMOUS (AG). From both petunia and cucumber, two MADS box genes have been isolated....
Colombo, L, Franken, J, Van Der Krol, A R, Wittich, P E, Dons, H J, Angenent, G C
A maternally determined seed defect has been obtained by downregulation of the petunia MADS box genes Floral Binding Protein 7 (FBP7) and FBP11. These genes have been previously shown to play central...
Differential expression of two MADS box genes in wild-type and mutant petunia flowers.
Angenent, G C, Busscher, M, Franken, J, Mol, J N, Van Tunen, A J
We isolated and characterized two flower-specific genes from petunia. The protein products of these genes, designated floral binding protein 1 (FBP1) and 2 (FBP2), are putative transcription factors...
Angenent, G C, Busscher, M, Franken, J, Dons, H J, Van Tunen, A J
The petunia MADS box floral binding protein (fbp) gene 1 represents a class B homeotic gene determining the identity of second and third floral whorl organs. Suppression of fbp1, which is highly...
A novel class of MADS box genes is involved in ovule development in petunia.
Angenent, G C, Franken, J, Busscher, M, Van Dijken, A, Van Went, J L, Dons, H J, ...
We isolated and characterized two ovule-specific MADS box cDNAs from petunia, designated floral binding protein (fbp) genes 7 and 11. The putative protein products of these genes have approximately...
The petunia MADS box gene FBP11 determines ovule identity.
Colombo, L, Franken, J, Koetje, E, Van Went, J, Dons, H J, Angenent, G C, ...
In contrast to the wealth of information relating to genes regulating floral meristem and floral organ identity, only limited data are available concerning genes that are involved in determining and...
RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.
Angenent, G C, Linthorst, H J, Van Belkum, A F, Cornelissen, B J, Bol, J F
The sequence of the 3'-terminal 1210 nucleotides of RNA 1 and the complete sequence of 3389 nucleotides of RNA 2 of tobacco rattle virus (TRV) strain TCM has been deduced. The sequence of the...
Kater, M M, Colombo, L, Franken, J, Busscher, M, Masiero, S, Van Lookeren Campagne, M M, ...
The C function in Arabidopsis, which specifies stamen and carpel identity, is represented by a single gene called AGAMOUS (AG). From both petunia and cucumber, two MADS box genes have been isolated....
Colombo, L, Franken, J, Van Der Krol, A R, Wittich, P E, Dons, H J, Angenent, G C
A maternally determined seed defect has been obtained by downregulation of the petunia MADS box genes Floral Binding Protein 7 (FBP7) and FBP11. These genes have been previously shown to play central...
Differential expression of two MADS box genes in wild-type and mutant petunia flowers.
Angenent, G C, Busscher, M, Franken, J, Mol, J N, Van Tunen, A J
We isolated and characterized two flower-specific genes from petunia. The protein products of these genes, designated floral binding protein 1 (FBP1) and 2 (FBP2), are putative transcription factors...
Angenent, G C, Busscher, M, Franken, J, Dons, H J, Van Tunen, A J
The petunia MADS box floral binding protein (fbp) gene 1 represents a class B homeotic gene determining the identity of second and third floral whorl organs. Suppression of fbp1, which is highly...
A novel class of MADS box genes is involved in ovule development in petunia.
Angenent, G C, Franken, J, Busscher, M, Van Dijken, A, Van Went, J L, Dons, H J, ...
We isolated and characterized two ovule-specific MADS box cDNAs from petunia, designated floral binding protein (fbp) genes 7 and 11. The putative protein products of these genes have approximately...
The petunia MADS box gene FBP11 determines ovule identity.
Colombo, L, Franken, J, Koetje, E, Van Went, J, Dons, H J, Angenent, G C, ...
In contrast to the wealth of information relating to genes regulating floral meristem and floral organ identity, only limited data are available concerning genes that are involved in determining and...
RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.
Angenent, G C, Linthorst, H J, Van Belkum, A F, Cornelissen, B J, Bol, J F
The sequence of the 3'-terminal 1210 nucleotides of RNA 1 and the complete sequence of 3389 nucleotides of RNA 2 of tobacco rattle virus (TRV) strain TCM has been deduced. The sequence of the...