Sebaihia, M., Wren, B.W., Mullany, P., Fairweather, N.F., Minton, N., Stabler, R., ...
We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists...
Jomaa, M., Yuste, J., Paton, James Cleland, Jones, Claire Louise, Dougan, G., Brown, J. S.
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Jomaa, M., Yuste, J., Paton, James Cleland, Jones, Claire Louise, Dougan, G., Brown, J. S.
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Cahill, R.J., Tan, S., Dougan, G., O’Gaora, P., Pickard, D., Kennea, N., ...
A large number of bacterial species have been identified in fetal membranes after preterm labour (PTL) associated with intrauterine infection by microbiological culture. In this study, we have...
Cahill, R.J., Tan, S., Dougan, G., OGaora, P., Pickard, D., Kennea, N., ...
A large number of bacterial species have been identified in fetal membranes after preterm labour (PTL) associated with intrauterine infection by microbiological culture. In this study, we have...
Jenkins, C., Perry, N. T., Cheasty, T., Shaw, D. J., Frankel, G., Dougan, G., ...
Certain strains of Shiga toxin-producing Escherichia coli (STEC) which do not have the locus of enterocyte effacement pathogenicity island carry the STEC autoagglutinating adhesin (saa) gene. The...
Jenkins, C., Perry, N. T., Cheasty, T., Shaw, D. J., Frankel, G., Dougan, G., ...
Certain strains of Shiga toxin-producing Escherichia coli (STEC) which do not have the locus of enterocyte effacement pathogenicity island carry the STEC autoagglutinating adhesin (saa) gene. The...
Refocusing of B-cell responses following a single amino acid substitution in an antigen (2001)
Chiesa, M.D., Martensen, P.M., Simmons, C., Porakishvili, N., Justensen, J., Dougan, G., ...
Intranasal immunization of BALB/c strain mice was carried out using baculovirus-derived human chorionic gonadotrophin (hCG) β-chain, together with Escherichia coli heat-labile enterotoxin....
Street, M., Herd, K., Londono, P., Doan, T., Dougan, G., Kast, W. M., ...
Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful-antibody responses when mice...
Street, M., Herd, K., Londono, P., Doan, T., Dougan, G., Kast, W. M., ...
Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful-antibody responses when mice...
Douce, G, Turcotte, C, Cropley, I, Roberts, M, Pizza, M, Domenghini, M, ...
A nontoxic mutant (LTK7) of the Escherichia coli heat-labile enterotoxin (LT) lacking ADP-ribosylating activity but retaining holotoxin formation was constructed. By using site-directed mutagenesis,...
Mastroeni, Pietro, Clare, S., Khan, S., Harrison, J. A., Hormaeche, C. E., Okamura, H., ...
Spleen and peritoneal macrophages obtained from innately resistant A/J mice released low levels of interleukin 18 (IL-18) upon infection with Salmonella typhimurium C5 RP4. Incubating the cells with...
Mastroeni, Pietro, Simmons, C., Fowler, R., Hormaeche, C. E., Dougan, G.
In the present study we evaluated the role of B cells in acquired immunity to Salmonella infection by using gene-targeted B-cell-deficient innately susceptible mice on a C57BL/6 background...
Degnan, B. A., Fontaine, M. C., Doebereiner, A. H., Lee, J. J., Mastroeni, P., Dougan, G., ...
An isogenic mutant of Streptococcus pyogenes Manfredo that lacks the ability to make streptococcal acid glycoprotein (SAGP) has been constructed by inserting a deletion in the sagp gene using the...
Mastroeni, Pietro, Harrison, J. A., Robinson, J. H., Clare, S., Khan, S., Maskell, D. J., ...
The attenuated S. typhimurium SL3261 (aroA) strain causes mild infections in BALB/c mice. We were able to exacerbate the disease by administering anti-interleukin-12 (IL-12) antibodies, resulting in...
Atkins, T., Prior, R. G., Mack, K., Russell, P., Nelson, M., Oyston, P. C. F., ...
Using a transposon mutagenesis approach, we have identified a mutant of Burkholderia pseudomallei that is auxotrophic for branched chain amino acids. The transposon was shown to have interrupted the...
Lodge, J, Douce, G R, Amin, I I, Bolton, A J, Martin, G D, Chatfield, S, ...
TnphoA transposon insertion mutants of phoN-negative derivatives of Salmonella typhimurium TML (of human gastroenteritic origin) were selected by growing mutagenized recipient bacteria under a...
Roberts, M, Bacon, A, Rappuoli, R, Pizza, M, Cropley, I, Douce, G, ...
We examined the capacity of a genetically detoxified derivative of pertussis toxin (PTX), PT-9K/129G, to act as a mucosal adjuvant for an intranasally (i.n.) administered tetanus vaccine. Groups of...
Characterization of recombinant tetanus toxin derivatives suitable for vaccine development.
Figueiredo, D, Turcotte, C, Frankel, G, Li, Y, Dolly, O, Wilkin, G, ...
Recombinant derivatives of tetanus toxin (TeTx) were isolated and used to immunize mice. Recombinant TeTx light chain, a derivative of fragment C that had lost the ability to bind neurons, and a...
Frankel, G, Candy, D C, Fabiani, E, Adu-Bobie, J, Gil, S, Novakova, M, ...
A eukaryotic cell-binding domain from the intimin (Int) polypeptide of enteropathogenic Escherichia coli O127 (EPEC) was investigated. Derivatives of the carboxy-terminal 280-amino-acid domains of...
Di Tommaso, A, Saletti, G, Pizza, M, Rappuoli, R, Dougan, G, Abrignani, S, ...
Immunization of the female reproductive tract is important for protection against sexually transmitted diseases and other pathogens of the reproductive tract. However, intravaginal immunization with...
Li, S R, Dorrell, N, Everest, P H, Dougan, G, Wren, B W
The high-temperature requirement (HtrA) family of stress response proteins are induced by different environmental stress conditions in a variety of bacteria and have been shown to contribute to the...
Immune response in mice following immunization with DNA encoding fragment C of tetanus toxin.
Anderson, R, Gao, X M, Papakonstantinopoulou, A, Roberts, M, Dougan, G
Tetanus toxin is a potent neurotoxin synthesized by Clostridium tetani. Immunization with fragment C protein, the nontoxic C-terminal domain of tetanus toxin, will protect mice against lethal...
Frankel, G, Phillips, A D, Novakova, M, Field, H, Candy, D C, Schauer, D B, ...
The formation of attaching and effacing (A/E) lesions is central to the pathogenesis of enteropathogenic Escherichia coli (EPEC)-mediated disease in humans and Citrobacter rodentium (formerly C....
Tacket, C O, Sztein, M B, Losonsky, G A, Wasserman, S S, Nataro, J P, Edelman, R, ...
A single-dose, oral Salmonella typhi vaccine strain has been sought as a carrier or vector of cloned genes encoding protective antigens of other pathogens. Such a hybrid vaccine, administered orally,...
Knutton, S, Adu-Bobie, J, Bain, C, Phillips, A D, Dougan, G, Frankel, G
Enteropathogenic Escherichia coli (EPEC) produces attaching and effacing (A/E) lesions in the intestinal mucosa. The intimate bacterial adhesion associated with A/E lesion formation is promoted by...
Intranasal immunogenicity and adjuvanticity of site-directed mutant derivatives of cholera toxin.
Douce, G, Fontana, M, Pizza, M, Rappuoli, R, Dougan, G
Genetically modified derivatives of cholera toxin (CT), harboring a single amino acid substitution in and around the NAD binding cleft of the A subunit, were isolated following site-directed...
Zhang-Barber, L, Turner, A K, Martin, G, Frankel, G, Dougan, G, Barrow, P A
Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated...
Frankel, G, Candy, D C, Everest, P, Dougan, G
Surface proteins called intimins (Int), which are homologous to the invasin protein (Inv) of Yersinia spp., play a role in inducing brush border damage, termed attachment and effacement, which...
Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions.
Chambers, M A, Dougan, G, Newman, J, Brown, F, Crowther, J, Mould, A P, ...
An RGD-containing epitope from the foot-and-mouth disease virus (FMDV) VP1 protein was inserted into the e1 loop of the hepatitis B virus core (HBc) protein. This chimeric protein was expressed at...
Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.
Garbe, T, Jones, C, Charles, I, Dougan, G, Young, D
The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the lambda gt11 vector....
Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis.
Maskell, D J, Morrissey, P, Dougan, G
The aroA locus of Bordetella pertussis, encoding 5-enolpyruvylshikimate 3-phosphate synthase, has been cloned into Escherichia coli by using a cosmid vector. The gene is expressed in E. coli and...
Moseley, S L, Dougan, G, Schneider, R A, Moon, H W
The genetic determinant for production of the adhesive antigen F41 was isolated from a porcine enterotoxigenic Escherichia coli strain by cosmid cloning. The cloned DNA included sequences homologous...
Identification and cloning of the genetic determinant that encodes for the K88ac adherence antigen.
Shipley, P L, Dougan, G, Falkow, S
Strains of Escherichia coli capable of causing diarrhea in young pigs are often able to proliferate in the upper small intestine of the infected animal due to the presence of a specific surface...
Escherichia coli K-12 minicells, harboring recombinant plasmids encoding polypeptides involved in the expression of K88ac adhesion pili on the bacterial cell surface, were labeled with...
Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tn1 and Tn3.
Dougan, G, Saul, M, Twigg, A, Gill, R, Sherratt, D
Escherichia coli K-12 minicells were employed to examine polypeptides encoded by plasmids carrying wild-type and mutant Tn1 or Tn3 transposition elements. Tn1- and Tn3-containing minicells express...
Gill, R, Heffron, F, Dougan, G, Falkow, S
The Tn1 and Tn3 elements are closely related transposons which carry the structural gene for ampicillin resistance. Two classes of deletion mutants of the plasmid pMB8::Tn3 (RSF1050) are unable to...
Coleman, K, Dougan, G, Arbuthnott, J P
A hemolysin determinant was cloned from Pseudomonas aeruginosa PA103 by inserting Sau3a-generated DNA fragments between the BamHI sites of the lambda replacement vector WL47.1. A 9.5-kilobase HindIII...
Changes in protein synthesis on mitomycin C induction of wild-type and mutant CloDF13 plasmids.
Mitomycin C treatment of Escherichia coli K-12 cells containing the nonconjugative plasmid CloDF13 resulted in inhibition of host chromosome protein synthesis and a high rate of synthesis of two...
Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16.
Comerford, S A, McCance, D J, Dougan, G, Tite, J P
There is strong evidence implicating human papillomavirus type 16 (HPV16) in the genesis of human genital cancer. Viral DNA has been identified in invasive carcinoma of the uterine cervix and in cell...
Strugnell, R, Dougan, G, Chatfield, S, Charles, I, Fairweather, N, Tite, J, ...
The P.69 Bordetella pertussis protective antigen was expressed by use of the trc promoter from the chromosome of a Salmonella typhimurium aro vaccine strain, BRD509, by integrating the prn gene,...
Chatfield, S N, Dorman, C J, Hayward, C, Dougan, G
A Salmonella typhimurium strain harboring stable mutations in both ompC and ompF was constructed from the mouse-virulent strain S. typhimurium SL1344. When administered orally to BALB/c mice the...
Construction and characterization in vivo of Bordetella pertussis aroA mutants.
Roberts, M, Maskell, D, Novotny, P, Dougan, G
A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector...
Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier.
Fairweather, N F, Chatfield, S N, Makoff, A J, Strugnell, R A, Bester, J, Maskell, D J, ...
A Salmonella typhimurium aroA mutant has been used as a live carrier to immunize mice against tetanus. Plasmid pTETtac4, which expresses a 50-kilodalton fragment of tetanus toxin (fragment C) under...
O'Callaghan, D, Maskell, D, Liew, F Y, Easmon, C S, Dougan, G
Stable transposon-generated auxotrophic mutations in aroA, purA, and purE or aroA and purA together were introduced into Salmonella typhimurium strains which were virulent in mice. Strains harboring...
Chatfield, S N, Fernie, D S, Penn, C, Dougan, G
Eleven strains of Treponema hyodysenteriae isolated from pigs with swine dysentery were examined by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. T....
Virulence, persistence, and immunogenicity of Yersinia enterocolitica O:8 aroA mutants.
Bowe, F, O'Gaora, P, Maskell, D, Cafferkey, M, Dougan, G
The virulent Yersinia enterocolitica strain 8081 killed BALB/c mice within 5 days of oral infection with a 50% lethal dose of log10 7.1, whereas an aroA mutant of 8081, YAM.1, and the plasmidless...
In vivo properties of a cloned K88 adherence antigen determinant.
Dougan, G, Sellwood, R, Maskell, D, Sweeney, K, Liew, F Y, Beesley, J, ...
An Escherichia coli strain of serotype O9:K36:H19 harboring the K88 recombinant plasmid pMK005 was able to efficiently colonize the small bowel of young piglets after oral infection. The strain...
Kehoe, M, Duncan, J, Foster, T, Fairweather, N, Dougan, G
A fragment of Staphylococcus aureus DNA encoding the alpha-hemolysin determinant was cloned from strain Wood 46 by inserting Sau3A-generated genomic DNA fragments between the BamHI sites of the...
Fairweather, N, Kennedy, S, Foster, T J, Kehoe, M, Dougan, G
A DNA sequence encoding Staphylococcus aureus alpha-hemolysin, which had been previously cloned and mapped in Escherichia coli K-12, was introduced into Bacillus subtilis BD170 and several strains of...
Antibiotic resistance in Providencia stuartii isolated in hospitals.
McHale, P J, Keane, C T, Dougan, G
A total of 238 isolates of Providencia stuartii obtained from infected patients in six Dublin hospitals were grouped by using serological and bacteriocin typing methods and tested for sensitivity to...
Charles, I G, Dougan, G, Pickard, D, Chatfield, S, Smith, M, Novotny, P, ...
Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein...
Pickard, D, Li, J, Roberts, M, Maskell, D, Hone, D, Levine, M, ...
The ompB operon, comprising the ompR and envZ genes, was cloned from a Salmonella typhi Ty2 cosmid bank and characterized by DNA sequence analysis. The S. typhi ompR and envZ genes contained open...
Isolation of orally attenuated Salmonella typhimurium following TnphoA mutagenesis.
Miller, I, Maskell, D, Hormaeche, C, Johnson, K, Pickard, D, Dougan, G
One hundred fifty Tn5 IS50L::phoA (TnphoA) mutants of a mouse-virulent, nalidixic acid-resistant (Nalr), prototrophic Salmonella typhimurium strain, C5 Nalr, were isolated. None of the mutants were...
Dorman, C J, Chatfield, S, Higgins, C F, Hayward, C, Dougan, G
The ompC, ompD, and ompF genes encode the three major porins of Salmonella typhimurium. ompR encodes a positive regulator required for the expression of ompC and ompF. Transposon-generated mutations...
Shaw, D. J., Jenkins, C., Pearce, M. C., Cheasty, T., Gunn, G. J., Dougan, G., ...
Rectal fecal samples were taken once a week from 49 calves on the same farm. In addition, the dams of the calves were sampled at the time of calf birth and at the end of the study. Strains of...
Moore, J D, Lamb, H K, Garbe, T, Servos, S, Dougan, G, Charles, I G, ...
The aroQ gene of Mycobacterium tuberculosis, encoding a type-II 3-dehydroquinase, and the aroD gene of Salmonella typhi, encoding a type-I 3-dehydroquinase, have been highly overexpressed in...
Knutton, S, Rosenshine, I, Pallen, M J, Nisan, I, Neves, B C, Bain, C, ...
Enteropathogenic Escherichia coli (EPEC), like many bacterial pathogens, employ a type III secretion system to deliver effector proteins across the bacterial cell. In EPEC, four proteins are known to...
Kehoe, M, Winther, M, Dougan, G
The determinant for the K88ac adherence antigen of porcine enterotoxigenic Escherichia coli has been cloned previously onto the vector plasmid pBR322 to form the K88ac-pBR322 hybrid plasmid pMK005...
Douce, G, Turcotte, C, Cropley, I, Roberts, M, Pizza, M, Domenghini, M, ...
A nontoxic mutant (LTK7) of the Escherichia coli heat-labile enterotoxin (LT) lacking ADP-ribosylating activity but retaining holotoxin formation was constructed. By using site-directed mutagenesis,...
Mastroeni, Pietro, Clare, S., Khan, S., Harrison, J. A., Hormaeche, C. E., Okamura, H., ...
Spleen and peritoneal macrophages obtained from innately resistant A/J mice released low levels of interleukin 18 (IL-18) upon infection with Salmonella typhimurium C5 RP4. Incubating the cells with...
Mastroeni, Pietro, Simmons, C., Fowler, R., Hormaeche, C. E., Dougan, G.
In the present study we evaluated the role of B cells in acquired immunity to Salmonella infection by using gene-targeted B-cell-deficient innately susceptible mice on a C57BL/6 background...
Degnan, B. A., Fontaine, M. C., Doebereiner, A. H., Lee, J. J., Mastroeni, P., Dougan, G., ...
An isogenic mutant of Streptococcus pyogenes Manfredo that lacks the ability to make streptococcal acid glycoprotein (SAGP) has been constructed by inserting a deletion in the sagp gene using the...
Mastroeni, Pietro, Harrison, J. A., Robinson, J. H., Clare, S., Khan, S., Maskell, D. J., ...
The attenuated S. typhimurium SL3261 (aroA) strain causes mild infections in BALB/c mice. We were able to exacerbate the disease by administering anti-interleukin-12 (IL-12) antibodies, resulting in...
Atkins, T., Prior, R. G., Mack, K., Russell, P., Nelson, M., Oyston, P. C. F., ...
Using a transposon mutagenesis approach, we have identified a mutant of Burkholderia pseudomallei that is auxotrophic for branched chain amino acids. The transposon was shown to have interrupted the...
Lodge, J, Douce, G R, Amin, I I, Bolton, A J, Martin, G D, Chatfield, S, ...
TnphoA transposon insertion mutants of phoN-negative derivatives of Salmonella typhimurium TML (of human gastroenteritic origin) were selected by growing mutagenized recipient bacteria under a...
Roberts, M, Bacon, A, Rappuoli, R, Pizza, M, Cropley, I, Douce, G, ...
We examined the capacity of a genetically detoxified derivative of pertussis toxin (PTX), PT-9K/129G, to act as a mucosal adjuvant for an intranasally (i.n.) administered tetanus vaccine. Groups of...
Characterization of recombinant tetanus toxin derivatives suitable for vaccine development.
Figueiredo, D, Turcotte, C, Frankel, G, Li, Y, Dolly, O, Wilkin, G, ...
Recombinant derivatives of tetanus toxin (TeTx) were isolated and used to immunize mice. Recombinant TeTx light chain, a derivative of fragment C that had lost the ability to bind neurons, and a...
Frankel, G, Candy, D C, Fabiani, E, Adu-Bobie, J, Gil, S, Novakova, M, ...
A eukaryotic cell-binding domain from the intimin (Int) polypeptide of enteropathogenic Escherichia coli O127 (EPEC) was investigated. Derivatives of the carboxy-terminal 280-amino-acid domains of...
Di Tommaso, A, Saletti, G, Pizza, M, Rappuoli, R, Dougan, G, Abrignani, S, ...
Immunization of the female reproductive tract is important for protection against sexually transmitted diseases and other pathogens of the reproductive tract. However, intravaginal immunization with...
Li, S R, Dorrell, N, Everest, P H, Dougan, G, Wren, B W
The high-temperature requirement (HtrA) family of stress response proteins are induced by different environmental stress conditions in a variety of bacteria and have been shown to contribute to the...
Immune response in mice following immunization with DNA encoding fragment C of tetanus toxin.
Anderson, R, Gao, X M, Papakonstantinopoulou, A, Roberts, M, Dougan, G
Tetanus toxin is a potent neurotoxin synthesized by Clostridium tetani. Immunization with fragment C protein, the nontoxic C-terminal domain of tetanus toxin, will protect mice against lethal...
Frankel, G, Phillips, A D, Novakova, M, Field, H, Candy, D C, Schauer, D B, ...
The formation of attaching and effacing (A/E) lesions is central to the pathogenesis of enteropathogenic Escherichia coli (EPEC)-mediated disease in humans and Citrobacter rodentium (formerly C....
Tacket, C O, Sztein, M B, Losonsky, G A, Wasserman, S S, Nataro, J P, Edelman, R, ...
A single-dose, oral Salmonella typhi vaccine strain has been sought as a carrier or vector of cloned genes encoding protective antigens of other pathogens. Such a hybrid vaccine, administered orally,...
Knutton, S, Adu-Bobie, J, Bain, C, Phillips, A D, Dougan, G, Frankel, G
Enteropathogenic Escherichia coli (EPEC) produces attaching and effacing (A/E) lesions in the intestinal mucosa. The intimate bacterial adhesion associated with A/E lesion formation is promoted by...
Intranasal immunogenicity and adjuvanticity of site-directed mutant derivatives of cholera toxin.
Douce, G, Fontana, M, Pizza, M, Rappuoli, R, Dougan, G
Genetically modified derivatives of cholera toxin (CT), harboring a single amino acid substitution in and around the NAD binding cleft of the A subunit, were isolated following site-directed...
Zhang-Barber, L, Turner, A K, Martin, G, Frankel, G, Dougan, G, Barrow, P A
Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated...
Frankel, G, Candy, D C, Everest, P, Dougan, G
Surface proteins called intimins (Int), which are homologous to the invasin protein (Inv) of Yersinia spp., play a role in inducing brush border damage, termed attachment and effacement, which...
Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions.
Chambers, M A, Dougan, G, Newman, J, Brown, F, Crowther, J, Mould, A P, ...
An RGD-containing epitope from the foot-and-mouth disease virus (FMDV) VP1 protein was inserted into the e1 loop of the hepatitis B virus core (HBc) protein. This chimeric protein was expressed at...
Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.
Garbe, T, Jones, C, Charles, I, Dougan, G, Young, D
The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the lambda gt11 vector....
Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis.
Maskell, D J, Morrissey, P, Dougan, G
The aroA locus of Bordetella pertussis, encoding 5-enolpyruvylshikimate 3-phosphate synthase, has been cloned into Escherichia coli by using a cosmid vector. The gene is expressed in E. coli and...
Moseley, S L, Dougan, G, Schneider, R A, Moon, H W
The genetic determinant for production of the adhesive antigen F41 was isolated from a porcine enterotoxigenic Escherichia coli strain by cosmid cloning. The cloned DNA included sequences homologous...
Identification and cloning of the genetic determinant that encodes for the K88ac adherence antigen.
Shipley, P L, Dougan, G, Falkow, S
Strains of Escherichia coli capable of causing diarrhea in young pigs are often able to proliferate in the upper small intestine of the infected animal due to the presence of a specific surface...
Escherichia coli K-12 minicells, harboring recombinant plasmids encoding polypeptides involved in the expression of K88ac adhesion pili on the bacterial cell surface, were labeled with...
Kehoe, M, Winther, M, Dougan, G
The determinant for the K88ac adherence antigen of porcine enterotoxigenic Escherichia coli has been cloned previously onto the vector plasmid pBR322 to form the K88ac-pBR322 hybrid plasmid pMK005...
Polypeptides expressed in Escherichia coli K-12 minicells by transposition elements Tn1 and Tn3.
Dougan, G, Saul, M, Twigg, A, Gill, R, Sherratt, D
Escherichia coli K-12 minicells were employed to examine polypeptides encoded by plasmids carrying wild-type and mutant Tn1 or Tn3 transposition elements. Tn1- and Tn3-containing minicells express...
Gill, R, Heffron, F, Dougan, G, Falkow, S
The Tn1 and Tn3 elements are closely related transposons which carry the structural gene for ampicillin resistance. Two classes of deletion mutants of the plasmid pMB8::Tn3 (RSF1050) are unable to...
Coleman, K, Dougan, G, Arbuthnott, J P
A hemolysin determinant was cloned from Pseudomonas aeruginosa PA103 by inserting Sau3a-generated DNA fragments between the BamHI sites of the lambda replacement vector WL47.1. A 9.5-kilobase HindIII...
Changes in protein synthesis on mitomycin C induction of wild-type and mutant CloDF13 plasmids.
Mitomycin C treatment of Escherichia coli K-12 cells containing the nonconjugative plasmid CloDF13 resulted in inhibition of host chromosome protein synthesis and a high rate of synthesis of two...
Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16.
Comerford, S A, McCance, D J, Dougan, G, Tite, J P
There is strong evidence implicating human papillomavirus type 16 (HPV16) in the genesis of human genital cancer. Viral DNA has been identified in invasive carcinoma of the uterine cervix and in cell...
Strugnell, R, Dougan, G, Chatfield, S, Charles, I, Fairweather, N, Tite, J, ...
The P.69 Bordetella pertussis protective antigen was expressed by use of the trc promoter from the chromosome of a Salmonella typhimurium aro vaccine strain, BRD509, by integrating the prn gene,...
Chatfield, S N, Dorman, C J, Hayward, C, Dougan, G
A Salmonella typhimurium strain harboring stable mutations in both ompC and ompF was constructed from the mouse-virulent strain S. typhimurium SL1344. When administered orally to BALB/c mice the...
Construction and characterization in vivo of Bordetella pertussis aroA mutants.
Roberts, M, Maskell, D, Novotny, P, Dougan, G
A DNA fragment encoding a kanamycin resistance determinant was used to insertionally inactivate the cloned aroA gene of Bordetella pertussis in Escherichia coli K-12, and a conjugative shuttle vector...
Oral vaccination of mice against tetanus by use of a live attenuated Salmonella carrier.
Fairweather, N F, Chatfield, S N, Makoff, A J, Strugnell, R A, Bester, J, Maskell, D J, ...
A Salmonella typhimurium aroA mutant has been used as a live carrier to immunize mice against tetanus. Plasmid pTETtac4, which expresses a 50-kilodalton fragment of tetanus toxin (fragment C) under...
O'Callaghan, D, Maskell, D, Liew, F Y, Easmon, C S, Dougan, G
Stable transposon-generated auxotrophic mutations in aroA, purA, and purE or aroA and purA together were introduced into Salmonella typhimurium strains which were virulent in mice. Strains harboring...
Chatfield, S N, Fernie, D S, Penn, C, Dougan, G
Eleven strains of Treponema hyodysenteriae isolated from pigs with swine dysentery were examined by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. T....
Virulence, persistence, and immunogenicity of Yersinia enterocolitica O:8 aroA mutants.
Bowe, F, O'Gaora, P, Maskell, D, Cafferkey, M, Dougan, G
The virulent Yersinia enterocolitica strain 8081 killed BALB/c mice within 5 days of oral infection with a 50% lethal dose of log10 7.1, whereas an aroA mutant of 8081, YAM.1, and the plasmidless...
In vivo properties of a cloned K88 adherence antigen determinant.
Dougan, G, Sellwood, R, Maskell, D, Sweeney, K, Liew, F Y, Beesley, J, ...
An Escherichia coli strain of serotype O9:K36:H19 harboring the K88 recombinant plasmid pMK005 was able to efficiently colonize the small bowel of young piglets after oral infection. The strain...
Kehoe, M, Duncan, J, Foster, T, Fairweather, N, Dougan, G
A fragment of Staphylococcus aureus DNA encoding the alpha-hemolysin determinant was cloned from strain Wood 46 by inserting Sau3A-generated genomic DNA fragments between the BamHI sites of the...
Fairweather, N, Kennedy, S, Foster, T J, Kehoe, M, Dougan, G
A DNA sequence encoding Staphylococcus aureus alpha-hemolysin, which had been previously cloned and mapped in Escherichia coli K-12, was introduced into Bacillus subtilis BD170 and several strains of...
Antibiotic resistance in Providencia stuartii isolated in hospitals.
McHale, P J, Keane, C T, Dougan, G
A total of 238 isolates of Providencia stuartii obtained from infected patients in six Dublin hospitals were grouped by using serological and bacteriocin typing methods and tested for sensitivity to...
Charles, I G, Dougan, G, Pickard, D, Chatfield, S, Smith, M, Novotny, P, ...
Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein...
Pickard, D, Li, J, Roberts, M, Maskell, D, Hone, D, Levine, M, ...
The ompB operon, comprising the ompR and envZ genes, was cloned from a Salmonella typhi Ty2 cosmid bank and characterized by DNA sequence analysis. The S. typhi ompR and envZ genes contained open...
Isolation of orally attenuated Salmonella typhimurium following TnphoA mutagenesis.
Miller, I, Maskell, D, Hormaeche, C, Johnson, K, Pickard, D, Dougan, G
One hundred fifty Tn5 IS50L::phoA (TnphoA) mutants of a mouse-virulent, nalidixic acid-resistant (Nalr), prototrophic Salmonella typhimurium strain, C5 Nalr, were isolated. None of the mutants were...
Dorman, C J, Chatfield, S, Higgins, C F, Hayward, C, Dougan, G
The ompC, ompD, and ompF genes encode the three major porins of Salmonella typhimurium. ompR encodes a positive regulator required for the expression of ompC and ompF. Transposon-generated mutations...
Shaw, D. J., Jenkins, C., Pearce, M. C., Cheasty, T., Gunn, G. J., Dougan, G., ...
Rectal fecal samples were taken once a week from 49 calves on the same farm. In addition, the dams of the calves were sampled at the time of calf birth and at the end of the study. Strains of...
Moore, J D, Lamb, H K, Garbe, T, Servos, S, Dougan, G, Charles, I G, ...
The aroQ gene of Mycobacterium tuberculosis, encoding a type-II 3-dehydroquinase, and the aroD gene of Salmonella typhi, encoding a type-I 3-dehydroquinase, have been highly overexpressed in...
Knutton, S, Rosenshine, I, Pallen, M J, Nisan, I, Neves, B C, Bain, C, ...
Enteropathogenic Escherichia coli (EPEC), like many bacterial pathogens, employ a type III secretion system to deliver effector proteins across the bacterial cell. In EPEC, four proteins are known to...
Xu, D, McSorley, S J, Chatfield, S N, Dougan, G, Liew, F Y
The gene encoding the Leishmania major (L. major) promastigote surface glycoprotein, gp63, was introduced into the Salmonella typhimurium (S. typhimurium) aroA- aroD- live oral vaccine strain BRD509...
Tite, J P, Gao, X M, Hughes-Jenkins, C M, Lipscombe, M, O'Callaghan, D, Dougan, G, ...
A plasmid encoding the influenza nucleoprotein gene from A/NT/60/68 virus was transduced into the attenuated Salmonella typhimurium aroA- strain SL3261. The bacterial vector expressing the viral gene...
Tite, J P, Hughes-Jenkins, C, O'Callaghan, D, Dougan, G, Russell, S M, Gao, X M, ...
Protection against influenza A virus infection in mice immunized with recombinant nucleoprotein (rNP) was studied. Nucleoprotein-immune mice were protected against a lethal challenge with A/Puerto...
O'Callaghan, D, Maskell, D, Tite, J, Dougan, G
Two near isogenic strains of Salmonella typhimurium HWSH, stably mutated in either the aroA gene affecting the biosynthesis of aromatic compounds, or the purA gene affecting the biosynthesis of...
Wain, J, House, D, Pickard, D, Dougan, G, Frankel, G
In this review we summarize recent genomic studies that shed light on the mechanism through which pathogenic Escherichia coli and Salmonella enterica have evolved. We show how acquisition of DNA at...
DNA topology and adaptation of Salmonella typhimurium to an intracellular environment.
Marshall, D G, Bowe, F, Hale, C, Dougan, G, Dorman, C J
The expression of genes coding for determinants of DNA topology in the facultative intracellular pathogen Salmonella typhimurium was studied during adaptation by the bacteria to the intracellular...
The immune responses to bacterial antigens encountered in vivo at mucosal surfaces.
Dougan, G, Ghaem-Maghami, M, Pickard, D, Frankel, G, Douce, G, Clare, S, ...
Mammals have evolved a sophisticated immune system for handling antigens encountered at their mucosal surfaces. The way in which mucosally delivered antigens are handled influences our ability to...
Phillips, A, Navabpour, S, Hicks, S, Dougan, G, Wallis, T, Frankel, G
BACKGROUND—Enterohaemorrhagic Escherichia coli (EHEC) constitute a significant risk to human health worldwide, and infections, particularly with serogroup O157:H7, are associated with consumption...
Fitzhenry, R J, Pickard, D J, Hartland, E L, Reece, S, Dougan, G, Phillips, A D, ...
Background: Enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli epithelial cell adhesion is characterised by intimate attachment, and attaching and effacing (A/E) lesion formation....
Haque, A, Bowe, F, Fitzhenry, R J, Frankel, G, Thomson, M, Heuschkel, R, ...
Background: Salmonella enterica serovar typhimurium (Styphimurium) causes invasive gastroenteritis in humans, a disease involving significant penetration of the intestinal mucosa. However, few...