G. Schatz

Details der Publikationsliste

Zeitraum

1966 - 2008

Anzahl

434

Co-Autoren

Applying Shower Development Universality to KASCADE Data (2008)

Apel, W. D., Badea, A. F., Bekk, K., Bluemer, J., Boos, E., Bozdog, H., ...

On basis of the theorem of a universal shower development stating that a hadronically generated extensive air shower is completely described by the primary energy, the position of the shower maximum...

Composition and energy spectra of elemental groups around the knee: Results from KASCADE (2008)

H. Ulrich, T. Antoni, W. D. Apel, F. Badea, K. Bekk, A. Bercuci, ...

A composition analysis of KASCADE air shower data is performed by means of unfolding the two-dimensional electron-muon number spectrum in energy spectra of 5 elemental groups. The results of the...

Galactic cosmic rays and the knee- Results from the KASCADE experiment 1 (2008)

J. R. Hör, W. D. Apel, F. Badea, K. Bekk, A. Bercuci, J. Blümer, ...

Abstract. Results of the KASCADE air shower experiment investigating the origin of cosmic rays in the energy region from 10 13 to 10 17 eV are presented. Attention is drawn on the investigation of...

Search for Extremely High Energy Gamma Rays with the KASCADE Experiment (2008)

G. Schatz, F. Feßler, T. Antoni, W. D. Apel, F. Badea, K. Bekk, ...

Data observed with the KASCADE extensive air shower experiment have been analyzed with respect to a possible contribution by primary gamma rays in the energy range of 0.3 to 10 PeV. Possible gamma...

28th International Cosmic Ray Conference 17 Analysis of Air Showers at the Trigger Threshold of KASCADE (2008)

J. Scholz, T. Antoni, W. D. Apel, F. Badea, K. Bekk, A. Bercuci, ...

The KASCADE experiment measures extensive air showers. It is 100% efficient for showers which are induced by primary particles with energies above 10 15 eV to pursue its main goal, the examination of...

28th International Cosmic Ray Conference 1595 Investigation of Geometrical Structures in the Hadronic (2008)

Shower Core, A. Iwan, T. Antoni, W. D. Apel, F. Badea, K. Bekk, ...

The geometrical structure of high-energy hadrons in shower cores measured with the KASCADE calorimeter is analyzed. The angular correlation, especially the degree of alignment, is quantified by means...

KASCADE Collaboration (2008)

T. Antoni A, F. Badea B, K. Bekk A, K. Bernlohr A, H. Blumer A, ...

non-parametric approach to infer the energy spectrum

28th International Cosmic Ray Conference 101 A Measurement of the Energy Spectrum of Unaccompanied (2008)

M. Müller, T. Antoni, W. D. Apel, F. Badea, K. Bekk, A. Bercuci, ...

The energy spectrum of unaccompanied hadrons is measured with the large hadron calorimeter of the KASCADE experiment. From the measured flux at detector level the primary proton spectrum at the top...

Cosmic (2007)

A. Haungs, T. Antoni, W. D. Apel, F. Badea, K. Bekk, I. M. Brancus, ...

ray physics around the knee with the KASCADE experiment ( ∗)

Proceedings of ICRC 2001: 814 c (2007)

Copernicus Gesellschaft Icrc, I. M. Brancus, C. Büttner, A. Chilingarian, K. Daumiller, P. Doll, ...

A Muon Tracking Detector has been built up in an underground tunnel north of the centre of the KASCADE experiment. It measures muons and their directions in EAS by using Limited Streamer Tubes. For...

Proceedings of ICRC 2001: 1418 c (2007)

Copernicus Gesellschaft Icrc, A. Iwan, T. Antoni, W. D. Apel, F. Badea, K. Bekk, ...

In this analysis the structure of hadronic shower cores is investigated with the KASCADE calorimeter. The energy, position, and direction of incidence for individual hadrons above 50 GeV are...

Proceedings of ICRC 2001: 88 c (2007)

Copernicus Gesellschaft Icrc, M. Roth, T. Antoni, W. D. Apel, F. Badea, K. Bekk, ...

The data measured by the KASCADE (KArlsruhe Shower Core and Array DEtector) experiment are the basis for a multi-component analysis with the aim to determine the mass composition of the primary...

Proceedings of ICRC 2001: 153 c (2007)

Copernicus Gesellschaft Icrc, C. Büttner, T. Antoni, W. D. Apel, F. Badea, K. Bekk, ...

The capability of the Muon Tracking Detector to measure the radial and tangential angles of muons in extensive air showers, in combination with the shower direction as determined by the scintillator...

Test of hadronic interaction models in the forward region with KASCADE event rates (2007)

T Antoni, F Badea, K Bekk, A Bercuci, K Bernlöhr, E Bollmann, ...

An analysis of muon and hadron rates observed in the central detector of the KASCADE experiment has been carried out. The data are compared with CORSIKA simulations employing the high-energy hadronic...

Proceedings of ICRC 2001: 2370 c (2007)

Copernicus Gesellschaft Icrc, F. Feßler, G. Schatz, T. Antoni, W. D. Apel, F. Badea, ...

Data observed with the KASCADE extensive air shower experiment have been analyzed with respect to a possible contribution by primary gamma rays in the energy range of 0.3 to 10 PeV. The study makes...

Proceedings of ICRC 2001: 201 c (2007)

Copernicus Gesellschaft Icrc, R. Haeusler, T. Antoni, W. D. Apel, F. Badea, K. Bekk, ...

Arrival time distributions of EAS muons carry information about the production profile of the EAS muonic component. The measured distributions are affected and distorted by various interwoven effects...

Proceedings of ICRC 2001: 233 c (2007)

Copernicus Gesellschaft Icrc, R. Glasstetter, R. Haeusler, A. Haungs, J. R. Hör, T. Holst, ...

The Monte Carlo program CORSIKA simulates the 4-dimensional evolution of extensive air showers in the atmosphere initiated by photons, hadrons or nuclei. It contains links to the hadronic interaction...

Extrem myonenarmer Luftschauer. (2006)

Schatz, G.

Frühjahrstagung DPG, Fachverband Teilchenphysik, Dortmund, 28.-31.März 2006

Longitudinalentwicklung von Luftschauern und ihre Streuung. (2006)

Schatz, G., Oehlschläger, J.

Frühjahrstagung DPG, Fachverband Teilchenphysik, Dortmund, 28.-31.März 2006

KASCADE (2005)

H. Ulrich, T. Antoni, W. D. Apel, A. F. Badea, K. Bekk, J. Blümer, ...

Reconstruction of energy spectra of elemental groups with

Test of hadronic interaction models with KASCADE. (2004)

Milke, J., Antoni, T., Apel, W.D., Badea, A.F., Bekk, K., Bercuci, A., ...

33rd Internat.Symp.on Multiparticle Dynamics, Krakow, PL, September 5-11, 2003

Astrophysics of the knee in the cosmic ray energy spectrum. (2004)

Haungs, A., Antoni, T., Apel, W.D., Badea, F., Bekk, K., Bercuci, A., ...

33rd Internat.Symp.on Multiparticle Dynamics, Krakow, PL, September 5-11, 2003

KASCADE: astrophysical results and tests of hadronic interaction models. (2004)

Haungs, A., Antoni, T., Apel, W.D., Badea, A.F., Bekk, K., Bercuci, A., ...

13th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Pylos, GR, September 6-12, 2004

Muon density spectra as probe of the muon component at air-shower simulations. (2003)

Haungs, A., Antoni, T., Apel, W.D., Badea, F., Bekk, K., Bercuci, A., ...

12th Internat.Symp.on Very High Energy Cosmic Ray Interactions (XII ISVHECRI), CERN, Geneve, CH, July 15-20, 2002

Test of hadronic interaction models with the KASCADE hadron calorimeter. (2003)

Milke, J., Antoni, T., Apel, W.D., Badea, F., Bekk, K., Bercuci, A., ...

12th Internat.Symp.on Very High Energy Cosmic Ray Interactions (XII ISVHECRI), CERN, Geneve, CH, July 15-20, 2002

Energy spectra of cosmic rays in the knee region. (2003)

Ulrich, H., Antoni, T., Apel, W.D., Badea, F., Bekk, K., Bercuci, A., ...

12th Internat.Symp.on Very High Energy Cosmic Ray Interactions (XII ISVHECRI), CERN, Geneve, CH, July 15-20, 2002

On the hadronic component of extensive air showers. (2003)

Hörandel, J.R., Antoni, T., Apel, W.D., Badea, F., Bekk, K., Bercuci, A., ...

12th Internat.Symp.on Very High Energy Cosmic Ray Interactions (XII ISVHECRI), CERN, Geneve, CH, July 15-20, 2002

A Search for Fine Structure of the Knee in EAS Size Spectra (2001)

Schatz, G.

28 size spectra of extensive air showers from 7 different experiments are analysed consistently. They are fitted by adjusting either 4 or 5 parameters: knee position, power law exponents above and...

Attenuation and Absorption Lengths of EAS measured with the KASCADE Experiment (2001)

G. Maier, T. Antoni, W.D. Apel, F. Badea, K. Bekk, A. Bercuci, ...

An analysis of attenuation and absorption lengths of the electron number using data of the KASCADE air shower experiment is presented. The following methods are used to determine these quantities:...

Large Muon Tracking Detector in the KASCADE EAS Experiment (2001)

J. Zabierowski, T. Antoni, W.D. Apel, F. Badea, K. Bekk, A. Bercuci, ...

Accurate measurements of the muon component in EAS are particularily important for determination of the primary CR spectrum and composition. Multiparameter analysis possibilities in KASCADE have been...

Inelasticity fluctuations and the efficiency of emulsion chambers for hadrons. (2001)

Schatz, G., Oehlschläger, J.

Proc.of the 11th Internat.Symp.on Very-High-Energy Cosmic Ray Interactions, Campinas, BR, July 17-21, 2000

Suche nach Gamma-Strahlung im PeV Bereich mit dem KASCADE-Experiment. (2001)

Fessler, F., Schatz, G.

Frühjahrstagung der DPG (mit Physik- und Buchaustellung), Bonn, 26.-29.März 2001

Die Abhängigkeit des 'Knies' im Größenspektrum ausgedehnter Luftschauer von der atmosphärischen Tiefe. (2001)

Schatz, G.

Frühjahrstagung der DPG (mit Physik- und Buchaustellung), Bonn, 26.-29.März 2001

Recent results of KASCADE. Phenomenology of extensive air showers. (2001)

Schatz, G.

Proc.of the 11th Internat.Symp.on Very-High-Energy Cosmic Ray Interactions, Campinas, BR, July 17-21, 2000

Search for PeV gamma rays with the KASCADE experiment. (2000)

Fessler, F., Schatz, G.

17th European Cosmic Ray Symp., Lodz, PL, July 23-28, 2000

Test of high-energy interaction models using the hadronic core of EAS (1999)

T. Antoni, W.D. Apel, K. Bekk, K. Bernlöhr, E. Bollmann, K. Daumiller, ...

. Using the large hadron calorimeter of the KASCADE experiment, hadronic cores of extensive air showers have been studied. The hadron lateral and energy distributions have been investigated in order...

Test of high-energy interaction models using the hadronic core of EAS (1999)

T Antoni, W D Apel, K. Bekk, K Bernlöhr, K Bernl Ohr, E Bollmann, ...

Using the large hadron calorimeter of the KASCADE experiment, hadronic cores of extensive air showers have been studied. The hadron lateral and energy distributions have been investigated in order to...

CORSIKA simulations for emulsion experiments on Mt.Chacaltaya. (1999)

Schatz, G., Oehlschläger, J.

Proc.of the 10th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Assergi, I, July 12-17, 1998

Chemiluminescent Reactions of Atomic Fluorine. (1998)

Schatz,G., Kaufman,M. J.

Visible or ultraviolet luminescence is observed in the reactions of atomic fluorine with a wide variety of organic and inorganic molecules. In most cases, the radiation is produced by reaction with...

Zur Fluktuation der Elektronenzahl protoneninduzierter ausgedehnter Luftschauer (EAS). (1998)

Schatz, G., Heck, D., Oehlschläger, J.

Frühjahrstagung des Fachverbandes Teilchenphysik der DPG, Freiburg, 23.-27.März 1998

Zur Bestimmung der Elektronen- und Myonenzahl in ausgedehnten Luftschauern (EAS). (1998)

Schatz, G., Oehlschläger, J.

Frühjahrstagung des Fachverbandes Teilchenphysik der DPG, Freiburg, 23.-27.März 1998

Physik der Höhenstrahlung. (1998)

Schatz, G.

Nachrichten - Forschungszentrum Karlsruhe, 30(1998) S.3-9

Some results of KASCADE. (1998)

Schatz, G.

Symp.Celebrating the Retirement of Professor Michael Hillas, The University of Leeds, GB, April 7, 1998

Results on cosmic ray composition around the knee and above or why do we know so little? (1998)

Schatz, G.

1st Workshop 'Physics at Cosmic Accelerators', Bad Liebenzell, April 1-5, 1998

Some metamorphoses of the knee. (1998)

Schatz, G.

Workshop 'The Anatomy of the Knee', Karlsruhe, December 9-10, 1998

Comparison of hadronic interaction models used in EAS simulations. (1997)

Knapp, J., Heck, D., Schatz, G.

Proc.of the 9th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Karlsruhe, August 19-23, 1996

Influence of hadronic interaction models on the development of EAS in Monte Carlo simulations. (1997)

Heck, D., Knapp, J., Schatz, G.

Proc.of the 9th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Karlsruhe, August 19-23, 1996

Das KASKADE-Experiment - Status und erste Experimente. (1997)

Schatz, G.

61. Physikertagung gemeinsam mit der Frühjahrstagung DPG, München, 17.-21.März 1997

Das KASCADE-Höhenstrahlexperiment. Status und erste Ergebnisse. (1997)

Schatz, G.

Vortr.: Physikalisches Kolloquium, Technische Hochschule Darmstadt, 2.Mai 1997

Very high energy cosmic ray interactions. (1997)

Rebel, H., Schatz, G., Knapp, J., [Hrsg.]

Proc.of the 9th Internat.Symp., Karlsruhe, August 19-23, 1996

The KASCADE cosmic ray experiment. (1997)

Schatz, G.

Vortr.: Universität Nagoya, J, 1.September 1997

Heavy element nucleosynthesis in stars. (1997)

Schatz, G.

Vortr.: Universität Nagoya, J, 5.September 1997

Das Luftschauer-Simulationsprogramm CORSIKA. (1996)

Knapp, J., Heck, D., Capdevielle, J.N., Oehlschläger, J., Schatz, G., Thouw, T., ...

Frühjahrstagung DPG, Teilchenphysik, Hamburg, 18.-23.März 1996

Das Höhenstrahlexperiment KASCADE. (1996)

Schatz, G.

Nachrichten - Forschungszentrum Karlsruhe, 28(1996) S.3-10

First results of the KASCADE experiment. (1996)

Schatz, G.

15th European Cosmic Ray Symp., Perpignan, F, August 26-30, 1996

Das KASCADE-Experiment zur Höhenstrahlung. (1995)

Schatz, G.

Vortr.: MPI für Kernphysik, Heidelberg, 23.Januar 1995

Experimentelle Methoden zur Bestimmung der Zusammensetzung. (1995)

Schatz, G.

Höhenstrahl-Workshop, Karlsruhe, 9.-10.März 1995

Range of muons in air. (1995)

Heck, D., Schatz, G.

Tagung der Astronomischen Gesellschaft, Bonn, 19.-22.September 1995

Unsolved problems of cosmic rays: origin and composition. (1994)

Schatz, G.

Vortr.: Universite Catolique de Louvain, B, 16.Dezember 1993

Relativistische Kern-Kern-Stösse und ausgedehnte Luftschauer. (1994)

Schatz, G., Thouw, T., Oehlschläger, J., Bekk, K., Werner, K.

Frühjahrstagung des Fachverbandes Teilchenphysik der DPG, Dortmund, 1.-4.März 1994

Ladungsaustausch von Pionen und die Entwicklung ausgedehnter Luftschauer. (1994)

Schatz, G., Thouw, T., Oehlschläger, J., Bekk, K., Werner, K.

Frühjahrstagung des Fachverbandes Teilchenphysik der DPG, Dortmund, 1.-4.März 1994

A look at the leading particle and inelasticity problem. (1994)

Bekk, K., Oehlschläger, J., Schatz, G., Thouw, T., Werner, K.

14th European Cosmic Ray Symp., Balatonfüred, H, August 28 - September 3, 1994

The KASCADE cosmic ray experiment. (1994)

Schatz, G.

Russische Höhenstrahlkonf., Moskva, GUS, 27.Juni - 2.Juli 1994

Recent accelerator measurements of cross sections. (1994)

Schatz, G.

Workshop on TeV Gamma-Ray Astrophysics, Heidelberg, October 3-7, 1994

KASCADE and the problem of primary cosmic ray composition. (1993)

Schatz, G.

Vortr.: Universitaet Bordeaux, F, 24.September 1993

An introduction to extensive air showers. (1993)

Schatz, G.

Proc.of the 2nd Gleb Wataghin School on High Energy Phenomenology, Campinas, BR, February 10-20, 1992

The Kascade cosmic ray experiment. (1993)

Schatz, G.

Interdisciplinary Science Reviews, 18(1993) S.306-12

Nuclei in cosmic rays. (1992)

Schatz, G.

Internat.Symp.on Nuclear Astrophysics: Nuclei in the Cosmos, Baden, A, June 18-22, 1990

Extensive air shower simulations with the CORSIKA program. (1992)

Capdevielle, J.N., Gabriel, P., Gils, H.J., Grieder, P., Heck, D., Knapp, J., ...

7th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Ann Arbor, Mich., June 21-27, 1992

Das KASCADE-Hoehenstrahlexperiment. (1991)

Schatz, G.

Vortr.: Workshop, Ahrenshoop, 17.-20.April 1990

Das KASCADE-Hoehenstrahl-Experiment. (1990)

Schatz, G.

Vortr.: Physikalisches Kolloquium, Universitaet Siegen, 23.November 1989 Vortr.: Universitaet Bern, CH, 11.Mai 1990

The Karlsruhe cosmic ray project KASCADE. (1990)

Doll, P., Engler, J., Gils, H.J., Heck, D., Heide, N., Keim, H., ...

Fruehjahrstagung DPG, Teilchenphysik, Hamburg, 21.-24.Maerz, 1990

Luftschauer Simulation fuer KASCADE. (1990)

Capdevielle, J.N., Gabriel, P., Gils, H.J., Grieder, P., Heck, D., Heide, N., ...

Fruehjahrstagung DPG, Teilchenphysik, Hamburg, 21.-24.Maerz, 1990

The Karlsruhe cosmic ray project KASCADE. (1990)

Doll, P., Engler, J., Gabriel, P., Gettert, M., Gils, H.J., Hanser, A., ...

12th European Cosmic Ray Symp., Nottingham, GB, July 15-21, 1990

The KASCADE extensive air shower experiment. (1990)

Schatz, G.

Vortr.: Universitaet Moskau, SU, 15.Juni 1990

E.A.S. simulations for the KASCADE project. (1990)

Capdevielle, J.N., Gabriel, P., Gils, H.J., Grieder, P., Heck, D., Heide, N., ...

6th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Tarbes, F, July 8-17, 1990

The KASCADE extensive air shower project. (1990)

Schatz, G.

6th Internat.Symp.on Very High Energy Cosmic Ray Interactions, Tarbes, F, July 8-17, 1990

`1`7`6Lu - an s-process thermometer. (1990)

Klay, N., Kaeppeler, F., Beer, H., Schatz, G.

Internat.Symp.on Capture Gamma Ray Spectroscopy, Asilomar, Calif., October 14-19, 1990

Das Karlsruher Luftschauerexperiment. (1989)

Engler, J., Gils, H.J., Heck, D., Heeringa, W., Klages, H.O., Knapp, J., ...

53.Physikertagung gemeinsam mit der Fruehjahrstagung DPG, Kern- und Hochenergie- physik, Energietechnik - Physikalische Grundlagen, Fachdidaktik der Physik, Gravitation und Relativitaetstheorie,...

Ein Multi-Transputer System fuer Monte-Carlo-Simulationen von ausgedehnten Luftschauern. (1989)

Gils, H.J., Heck, D., Oehlschlaeger, J., Schatz, G., Thouw, T., Merkel, A.

53.Physikertagung gemeinsam mit der Fruehjahrstagung DPG, Kern- und Hochenergie- physik, Energietechnik - Physikalische Grundlagen, Fachdidaktik der Physik, Gravitation und Relativitaetstheorie,...

Ausgedehnte Luftschauer und die Zusammensetzung der primaeren Hoehenstrahlung. (1989)

Schatz, G.

Vortr.: Max-Planck-Institut fuer Kernphysik, Heidelberg, 26.Januar 1989

Air shower simulations for the KASCADE project. (1989)

Schatz, G.

Cosmic Ray Cascade Simulation Workshop, Salt Lake City, Utah, March 2-3, 1989

Atomic beam, optogalvanic and stored ion spectroscopy for radioactive isotopes. (1988)

Meisel, G., Bekk, K., Rebel, H., Schatz, G.

Workshop on Modern Optics, Lasers and Laser Spectroscopy for Radioactive Isotopes, Kanpur, IND, January 23-29, 1987

Laserspektroskopische Messungen an gespeicherten radioaktiven Thorium-Ionen. (1988)

Bekk, K., Faubel, W., Goering, S., Kaelber, W., Meisel, G., Rebel, H., ...

Fruehjahrstagung DPG, Atomphysik, Massenspektrometrie, Molekuelphysik, Quantenoptik, Bonn, 21.-25.Maerz 1988

Ausgedehnte Luftschauer. (1988)

Schatz, G.

Expertentreffen Kernphysik, Schleching, 8.Maerz 1988

Laserspectroscopic studies of Am and Th isotopes. (1988)

Bekk, K., Faubel, W., Goering, S., Hanser, A., Kaelber, W., Meisel, G., ...

11th Internat.Conf.on Atomic Physics (ELICAP), Paris, F, July 4-8, 1988

Synthese schwerer Elemente in Sternen. (1988)

Schatz, G.

Vortr.: Physikalisches Kolloquium, Universitaet Frankfurt, 15.Juni 1988

Laborexperimente zur Entstehung schwerer Elemente in Sternen. (1987)

Schatz, G.

Vortr.: Institut fuer Strahlen- und Kernphysik, Universitaet Bonn, 13.November 1985

The level scheme of `1`7`6 Lu and its astrophysical implications. (1987)

Klay, N., Beer, H., Boerner, H., Kaeppeler, F., Schatz, G., Krusche, B.

Spring Meeting of Nuclear Physics Sections of the Deutsche Physikalische Gesellschaft, Nederlandse Natuurkundige Vereniging, Belgische Natuurkundige Vereniging, Societe Belge de Physique, Dansk...

Das 'Odd-Even Staggering' der nuklearen Ladungsgradien der Blei-Isotope. (1986)

Anselment, M., Faubel, W., Goering, S., Hanser, A., Meisel, G., Rebel, H., ...

50.Physikertagung gemeinsam mit der Fruehjahrstagung DPG, Atomphysik, Kern- und Hochenergiephysik, Molekuelphysik, Quantenoptik, der Arbeitsgemeinschaft Massenspektrometrie, Wechselwirkung von...

Nukleare Ladungsgradien und elektromagnetische Momente von Sn-Isotopen aus laserspektroskopischen Untersuchungen. (1986)

Anselment, M., Hanser, A., Hoeffgen, H., Goering, S., Meisel, G., Rebel, H., ...

50.Physikertagung gemeinsam mit der Fruehjahrstagung DPG, Atomphysik, Kern- und Hochenergiephysik, Molekuelphysik, Quantenoptik, der Arbeitsgemeinschaft Massenspektrometrie, Wechselwirkung von...

Nukleare Uhren und Thermometer der Astrophysik. (1986)

Schatz, G.

50.Physikertagung gemeinsam mit der Fruehjahrstagung DPG, Atomphysik, Kern- und Hochenergiephysik, Molekuelphysik, Quantenoptik, der Arbeitsgemeinschaft Massenspektrometrie, Wechselwirkung von...

Production of heavy elements by the s-process. (1986)

Schatz, G.

Vortr.: Internat.School of Nuclear Physics, Erice, I, April 2-14, 1986

Recent laser spectroscopy activities at KfK. (1986)

Anselment, M., Bekk, K., Chongkum, S., Goering, S., Hanser, A., Kaelber, W., ...

18th Meeting of the European Group for Atomic Spectroscopy (EGAS), Marburg, July 8-11, 1986

Laserspektroskopische Untersuchungen von Strontium-Isotopen. (1985)

Anselment, A., Chongkum, S., Hanser, A., Goering, S., Meisel, G., Rebel, H., ...

49.Physikertagung gemeinsam mit der Fruehjahrstagung DPG, Kern- und Hochenergiephysik, Didaktik der Physik, Energietechnik - Physikalische Grundlagen, Vakuumtechnik, Computer in der Technischen...

The `1`7`6Lu-`1`7`6Hf s-process chronometer and planned projects with a 4#pBGO detector. (1984)

Schatz, G.

Nuclear Astrophysics : Proc.of the 2nd workshop, Tegernsee, June 6-10, 1983

Laserspektroskopie an stabilen und radioaktiven Sn-Atomen. (1984)

Anselment, M., Hanser, A., Hoeffgen, J., Goering, S., Meisel, G., Rebel, H., ...

Fruehjahrstagung DPG, Atomphysik, Massenspektrometrie, Quantenoptik, Giessen, 19.-23.Maerz 1984.

Laserspektroskopische Untersuchungen an instabilen Bleiisotopen. (1983)

Thompson, R.C., Anselment, M., Bekk, K., Goering, S., Hanser, A., Meisel, G., ...

47. Physikertagung/Deutsche Physikalische Gesellschaft. Gemeinsam mit der Fruehjahrstagung der Fachgremien Atomphysik, Massenspektrometrie, Molekuelphysik..., Regensburg, 14.-18.Maerz 1983

Note on technetium in stars. (1983)

Schatz, G.

Astronomy and Astrophysics, 122(1983) S.327-29

Nuclear physics. (1983)

Aures, R., Beer, H., Beck, R., Bischoff, A., Buschmann, J., Dickmann, F., ...

In: Gemmeke, H.; Kaeppeler, F.; Weddigen, Ch.; [Hrsg.]

Laser spectroscopy. (1983)

Anselment, M., Bekk, K., Chongkum, S., Dembczynski, J., Goering, S., Hanser, A., ...

In: Gemmeke, H.; Kaeppeler, F.; Weddigen, Ch.; [Hrsg.]

Detectors. (1983)

Bischoff, A., Bluem, P., Brady, F.P., Cierjacks, S., Doll, P., Finckh, E., ...

In: Gemmeke, H.; Kaeppeler, F.; Weddigen, Ch.; [Hrsg.]

Neue Ergebnisse zum s-Prozess der stellaren Nukleosynthese. (1983)

Schatz, G.

Vortr.: MPI fuer Astrophysik, Muenchen, 29.November 1983

Laser induced fluorescence spectroscopy in atomic beams of radioactive nuclides. (1982)

Rebel, H., Schatz, G.

Conf.on Lasers in Nuclear Physics, Oak Ridge, Tenn., April 21-23, 1982. Invited talk

Medical and engineering applications of the Karlsruhe Isochronous Cyclotron. (1982)

Schatz, G.

Vortr.: University of Petroleum and Minerals, Dhahran, Saudi-Arabien, 28.Januar 1982

Nuclear charge radii of unstable nuclei from laser spectroscopy. (1982)

Schatz, G.

Vortr.: University of Petroleum and Minerals, Dhahran, Saudi-Arabien, 27.Januar 1982

Laser spectroscopy. (1982)

Andl, A., Bekk, K., Goering, S., Hanser, A., Nowicki, G., Rebel, H., ...

Beck, R.; Bueche, G.; Fluegge, G.; Kernert, N. [Hrsg.]

Technetium im s-Prozess. (1982)

Schatz, G.

Vortr.: Workshop ueber Fragen der nuklearen Astrophysik, Mainz, 13.-15.Oktober 1982

Laborexperimente zur Entstehung schwerer Elemente in Sternen. (1982)

Schatz, G.

Vortr.: MPI fuer Kernphysik, Heidelberg, 9.Dezember 1982

Der s-Prozess der Elementsynthese. (1981)

Schatz, G.

Expertentreffen fuer Kernphysik, Schleching, 4.-13.Maerz 1981

Industrial and environmental applications of cyclotrons. (1981)

Schatz, G.

9th Internat.Conf.on Cyclotrons and Their Applications, Caen, F, September 7-10, 1981

Bestimmung von Ladungsradien instabiler Kerne, eine Anwendung der Laserspektroskopie. (1981)

Schatz, G.

Vortr.: Gesellschaft fuer Schwerionenforschung, Darmstadt, 26.Mai 1981

Laser techniques for the detection of small numbers of atoms. Invited talk. (1981)

Schatz, G.

US-German Workshop on Radioactive Ion Beams and Small Cross Section Measurements, Columbus, Ohio, August 31 - September 4, 1981

Die Entstehung der schweren Elemente im stellaren s-Prozess. (1981)

Schatz, G.

Grundlagenforschung im Kernforschungszentrum Karlsruhe, Kernforschungszentrum Karlsruhe, 25.Juni 1981

Laser Spectroscopy of minute samples of radioactive alkaline earth atoms. (1980)

Schatz, G.

Vortr.: KVI, Universitaet Groningen, NL, February 11, 1980

Bestimmung der Kernladungsradien der Calcium-Isotope mit 40 (1980)

Andl, A., Bekk, K., Hanser, A., Nowicki, G., Rebel, H., Schatz, G.

Fruehjahrstagung DPG, Kernphysik, Muenchen, 17.-21. Maerz 1980. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd15(1980) S.1075

Isotopieverschiebung und Hyperfeinstruktur der Calcium-Resonanzlinie fuer die Isotope mit 40 (1980)

Andl, A., Bekk, K., Hanser, A., Nowicki, G., Rebel, H., Schatz, G.

Fruehjahrstagung DPG, Atomphysik, Bielefeld, 3.-7.Maerz 1980. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 15(1980) S.537

Laser spectroscopy of radioactive alkaline earth atoms. (1980)

Andl, A., Bekk, K., Goering, S., Hanser, A., Nowicki, G., Rebel, H., ...

Trends in Nuclear Structure Physics. Institute of Physics, Conf., Manchester, April 16-18, 1980

Laser spectroscopy and photon scattering. (1980)

Andl, A., Bekk, K., Feurer, B., Hanser, A., Nowicki, G., Rebel, H., ...

In: Annual Report. Teilinstitut Kernphysik des Instituts fuer Angewandte Kernphysik. (July 1, 1979 - June 30, 1980).

Laserspektroskopische Messungen von Isotopieverschiebung und Hyperfeinstruktur neutronenarmer Ba Isotope von A = 126 bis A = 138. (1979)

Bekk, K., Andl, A., Goering, S., Hanser, S., Nowicki, G., Rebel, H., ...

Fruehjahrstagung DPG, Atomphysik, Berlin, 5.-9.Maerz 1979. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 14(1979) S. 480

Nuclear charge radii and moments of neutron deficient Ba isotopes from high resolution laser spectroscopy. (1979)

Andl, A., Bekk, K., Goering, S., Hanser, A., Nowicki, G., Rebel, H., ...

Spring Meeting DPG,NNV,DFS,BNV-SBP, Nuclear Physics, Gent, Belgium, March 26-30, 1979. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 14(1979) S.824

Nuclear charge radii and moments of neutron deficient Ba isotopes from high resolution laser spectroscopy. (1979)

Andl, A., Bekk, K., Goering, S., Hanser, A., Nowicki, G., Rebel, H., ...

Gross Properties of Nuclei and Nuclear Excitations, Internat.Workshop VII, Hirschegg, Austria, January 15-27,1979

Atomic beam laser spectroscopy of radioactive Ba atoms. (1979)

Schatz, G.

Laser-Spectroscopy, 4.Internat.Conf., Rottach-Egern, June 11-15, 1979

Laserspectroscopy of short-lived isotopes. (1979)

Andl, A., Bekk, K., Feurer, B., Goering, S., Hanser, A., Nowicki, G., ...

In: Annual Report. Teilinstitut Kernphysik des Instituts fuer Angewandte Kernphysik (July 1,1978 - June 30,1979). KfK-2868 (Oktober 79) S.57-62

Laserspektroskopie an radioaktiven Atomen. (1979)

Schatz, G.

Vortrag in der KFA Juelich, Inst.f.Kernphysik, 26.November 1979

Laser spectroscopy of radioactive Ba atoms. (1979)

Schatz, G.

Vortrag, Inst.f.Physik, Jagellonian Univ., Krakau, Polen, December 14, 1979

Bestimmung der Isotopieverschiebung und Hyperfeinstruktur leichter Barium-Isotope mittels Laserspektroskopie. (1978)

Bekk, K., Nowicki, G., Goering, S., Hanser, A., Rebel, H., Schatz, G.

Fruehjahrstagung DPG, Atomphysik, Massenspektrometrie, Kurzzeitphysik, Molekuelphysik, Oberflaechenphysik, Duenne Schichten, Plasma-, Gasentladungsphysik, Quantenoptik, Muenchen, 6.-10.Maerz 1978....

Isotopieverschiebung und Hyperfeinstruktur neutronenarmer Barium-Isotope. (1978)

Nowicki, G., Bekk, K., Goering, S., Hanser, A., Rebel, H., Schatz, G.

Fruehjahrstagung DPG, Kernphysik, Heidelberg, 13.-17.Maerz 1978. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 13(1978) S.841

Erzeugung von Neutronen im keV-Bereich. (1978)

Kaeppeler, F., Schatz, G.

Fruehjahrstagung DPG, Kernphysik, Heidelberg, 13.-17.Maerz 1978. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 13(1978) S.945

Hochaufloesende Laserspektroskopie an radioaktiven Barium-Isotopen. (1978)

Schatz, G.

Institut fuer Experimentalphysik, Univ.Hamburg, 23.Januar 1978

Nuclear charge radii and moments of neutron deficient Ba-isotopes by high resolution laser spectroscopy. (1978)

Bekk, K., Goering, S., Hanser, A., Nowicki, G., Rebel, H., Schatz, G.

10.Conf.on Atomic Spectroscopy, European Group for Atomic Spectroscopy, European Physical Society, Muenchen, July 10-14, 1978

Spectroscopy of minute samples or how to look with lasers at nuclei. (1978)

Schatz, G.

Institut Max von Laue - Paul Langevin, Kolloquium, Grenoble, 11.Mai 1978

Untersuchung der Hyperfeinstruktur an radioaktiven Bariumatomen. (1978)

Schatz, G.

Vortrag, Kolloquium des Sonderforschungsbereiches 'Hyperfeinwechselwirkungen', FU Berlin, 27.April 1978

Laser spectroscopy. (1978)

Bekk, K., Feurer, B., Goering, S., Hanser, A., Nowicki, G., Rebel, H., ...

In: Annual Report. Teilinstitut Kernphysik. (July 1, 1977 - June 30, 1978).

Hochaufloesende Laserspektroskopie an leichten Barium-Isotopen. (1977)

Nowicki, G., Bekk, K., Goering, S., Hanser, A., Rebel, H., Schatz, G.

Fruehjahrstagung DPG, Atomphysik, Massenspektrometrie, Molekuelphysik, Quantenoptik, Mainz, 28.Februar - 5.Maerz 1977. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 12(1977) S.484

Hochaufloesende Laserspektroskopie an leichten Barium-Isotopen. (1977)

Schatz, G., Bekk, K., Goering, S., Hanser, A., Nowicki, G., Rebel, H.

Fruehjahrstagung DPG, Kernphysik, Konstanz, 21.-25.Maerz 1977. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 12(1977) S.889

High resolution laser spectroscopy of radioactive light barium isotopes. (1977)

Nowicki, G., Bekk, K., Goering, S., Hanser, A., Rebel, H., Schatz, G.

9.Annual Conference on Atomic Spectroscopy, EGAS, Cracow, Poland, July 12-15, 1977

Laser spectroscopy. (1977)

Bekk, K., Feurer, B., Goering, S., Hanser, A., Nowicki, G., Rebel, H., ...

In: Annual Report. Teilinstitut Kernphysik. (July 1, 1976 - June 30, 1977). KFK-2504 (Oktober 77) S.70-75

Basic nuclear physics at the cyclotron: Measurements of nuclear magnetic moments. (1977)

Bekk, K., Buchinger, F., Collignon, J., Goering, S., Graeff, G., Hanser, A., ...

In: F. Schulz, H. Schweickert [Hrsg.]: Operation of the Karlsruhe Isochronous Cyclotron in 1976.

Status Report of the Axial Injection System at the Karlsruhe Isochronous Cyclotron. (1976)

Haushahn, G., Moellenbeck, J., Schatz, G., Schulz, F., Schweickert, H.

Fruehjahrstagung DPG und OEPG, Kernphysik, Baden/Oesterr., 29. Maerz - 2. April 1976. Verhandlungen der Deutschen Physikalischen Gesellschaft, R.6, Bd 11(1976) S.809

Nuclear medicine. (1976)

Adam, W.E., Bitter, F., Goering, S., Hanser, A., Haushahn, G., Helus, F., ...

In: Operation of the Karlsruhe Isochronous Cyclotron in 1975.

Laser spectroscopy. (1976)

Feurer, B., Goering, S., Hanser, A., Nowicki, G., Rebel, H., Schatz, G.

In: Annual Report. Teilinstitut Kernphysik. (July 1, 1975 - June 30, 1976)

Nuclear medicine. (1976)

Adam, W.E., Bitter, F., Kampmann, H., Maier-Borst, W., Lambrecht, R.M., Wolf, A.P., ...

In: Operation of the Karlsruhe Isochronous Cyclotron in 1975.

Applications of Cyclotrons in Technical and Analytical Studies. (1975)

Gerve, A., Schatz, G.

7.Internat.Conference on Cyclotrons and their Applications, Zuerich, August 19-22, 1975

Computer Controlled Beam Diagnostics at the Karlsruhe Isochronous Cyclotron (1975)

Hartwig, D., Kappel, W., Kneis, W., Moellenbeck, J., Schatz, G., Schweickert, H.

7.International Conference on Cyclotrons and their Applications, Zuerich, August 19-22, 1975

Status Report of the Axial Injection System at the Karlsruhe Isochronous Cyclotron. (1975)

Haushahn, G., Moellenbeck, J., Schatz, G., Schulz, F., Schweickert, H.

7.International Conference on Cyclotrons and their Applications, Zuerich, August 19-22, 1975

Das Niveauschema von `1`3`5Pr: Hinweise auf prolate Deformation in Kernen mit ungerader Protonenzahl. (1974)

Wisshak, K., Buschmann, J., Faust, H., Habs, D., Klewe-Nebenius, H., Nowicki, G., ...

Fruehjahrstagung DPG, Kernphysik, Bochum, 4.-9.Maerz 1974. Verhandlungen der Deutschen Physikalischen Gesellschaft, R. 6, Bd 9(1974) S.80-81

Beschleunigung von `6Li`3`+-Ionen im Karlsruher Isochronzyklotron. (1974)

Biber, J., Haushahn, G., Kuhn, H., Moellenbeck, J., Schatz, G., Schulz, F., ...

Fruehjahrstagung DPG, Kernphysik, Bochum, 4.-9.Maerz 1974. Verhandlungen der Deutschen Physikalischen Gesellschaft, R. 6, Bd 9(1974) S.147-48

Ueberlegungen zum Entwurf eines Energieverlust-Spektrometers fuer das Isochron Zyklotron Karlsruhe. (1973)

Gils, H.J., Schatz, G.

Fruehjahrstagung DPG, Kernphysik, Heidelberg, 19.-23.Februar 1973. Verhandlungen der Deutschen Physikalischen Gesellschaft, Reihe 6, Bd 8(1973) S.33

Technische Entwicklungen am Karlsruher Isochronzyklotron. (1973)

Haushahn, G., Kappel, W.R., Kuhn, H., Moellenbeck, J., Schatz, G., Schulz, F., ...

Fruehjahrstagung DPG, Kernphysik, Heidelberg, 19.-23.Februar 1973. Verhandlungen der Deutschen Physikalischen Gesellschaft, Reihe 6, Bd 8 (1973) S.65

Gammaspektroskopische Experimente zur Untersuchung prolater, oblater und asymmetrischer Kernformen im Massenbereich mit 50 (1973)

Buschmann, J., Goering, S., Habs, D., Klewe-Nebenius, H., Loehken, R., Nowicki, G., ...

Fruehjahrstagung DPG, Kernphysik, Heidelberg, 19.-23.Februar 1973. Verhandlungen der Deutschen Physikalischen Gesellschaft, Reihe 6, Bd 8(1973) S.160

High Spin Bands of Odd Mass Nuclei with (59 (1973)

Habs, D., Nowicki, G., Klewe-Nebenius, H., Wisshak, K., Buschmann, J., Rebel, H., ...

International Conference on Nuclear Physics, Muenchen, August 27 - September 1, 1973

Operation of the Karlsruhe Isochronous Cyclotron in 1969. (1970)

Schatz, G., Schulz, F.

7.European Cyclotron Progress Meeting, Eindhoven, April 13-14,1970

A sectored isochronous ring for accelerating heavy ions (1969)

Schatz, G.

Nuclear Instruments and Methods, 67 (1969) S. 103-08

Cyclophilin catalyzes protein folding in yeast mitochondria.

Matouschek, A, Rospert, S, Schmid, K, Glick, B S, Schatz, G

Cyclophilins are a family of ubiquitous proteins that are the intracellular target of the immunosuppressant drug cyclosporin A. Although cyclophilins catalyze peptidylprolyl cis-trans isomerization...

The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast.

Lithgow, T, Junne, T, Suda, K, Gratzer, S, Schatz, G

We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses...

Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.

Kronidou, N G, Oppliger, W, Bolliger, L, Hannavy, K, Glick, B S, Schatz, G, ...

The protein import system of the yeast mitochondrial inner membrane includes at least three membrane proteins that presumably form a transmembrane channel as well as several chaperone proteins that...

Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria.

Rospert, S, Glick, B S, Jenö, P, Schatz, G, Todd, M J, Lorimer, G H, ...

Chaperonin 60 (cpn60) and chaperonin 10 (cpn10) constitute the chaperonin system in prokaryotes, mitochondria, and chloroplasts. In Escherichia coli, these two chaperonins are also termed groEL and...

Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane.

Scherer, P E, Manning-Krieg, U C, Jenö, P, Schatz, G, Horst, M

Import of proteins into mitochondria involves the cooperation of protein translocation systems in the outer and inner membranes. We have identified a 45-kDa protein at the protein import site of the...

Analysis of cleavage site mutations between the NC and PR Gag domains of Rous sarcoma virus.

Schatz, G, Pichova, I, Vogt, V M

In retroviruses, the viral protease (PR) is released as a mature protein by cleavage of Gag, Gag-Pro, or Gag-Pro-Pol precursor polypeptides. In avian sarcoma and leukemia viruses (ASLV), PR forms the...

Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.

Haucke, V, Ocana, C S, Hönlinger, A, Tokatlidis, K, Pfanner, N, Schatz, G

Mitochondrial NADH-cytochrome b5 reductase (Mcr1p) is encoded by a single nuclear gene and imported into two different submitochondrial compartments: the outer membrane and the intermembrane space....

Processing of avian retroviral gag polyprotein precursors is blocked by a mutation at the NC-PR cleavage site.

Burstein, H, Bizub, D, Kotler, M, Schatz, G, Vogt, V M, Skalka, A M

The avian sarcoma and leukosis viruses (ASLV) encode a protease (PR) at the C terminus of gag which in vivo catalyzes the processing of both gag and gag-pol precursors. The studies reported here were...

Properties of avian retrovirus particles defective in viral protease.

Stewart, L, Schatz, G, Vogt, V M

The structural and enzymatic components of retroviral cores are formed by proteolytic cleavage of precursor polypeptides, mediated by the viral protease (PR). We constructed an active-site mutation,...

Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.

Hwang, S T, Schatz, G

Work in several laboratories has established that import of proteins across the mitochondrial inner membrane requires an electrochemical potential across that membrane and cleavage of nucleoside...

Protein import into mitochondria: the requirement for external ATP is precursor-specific whereas intramitochondrial ATP is universally needed for translocation into the matrix.

Wachter, C, Schatz, G, Glick, B S

ATP is needed for the import of precursor proteins into mitochondria. However, the role of ATP and its site of action have been unclear. We have now investigated the ATP requirements for protein...

Sequences from a prokaryotic genome or the mouse dihydrofolate reductase gene can restore the import of a truncated precursor protein into yeast mitochondria.

Baker, A, Schatz, G

Sequences that are capable of restoring mitochondrial targeting to a truncated yeast cytochrome c oxidase subunit IV presequence are encoded within the genome of Escherichia coli and within the gene...

A yeast mutant lacking mitochondrial manganese-superoxide dismutase is hypersensitive to oxygen.

Van Loon, A P, Pesold-Hurt, B, Schatz, G

The nuclear gene for manganese-containing superoxide dismutase (MnSOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) of yeast mitochondria was mapped on chromosome VIII and inactivated by gene...

PROMITOCHONDRIA OF ANAEROBICALLY GROWN YEAST, IV. CONVERSION INTO RESPIRING MITOCHONDRIA*

Plattner, H., Salpeter, M. M., Saltzgaber, J., Schatz, G.

Promitochondria of anaerobically grown Saccharomyces cerevisiae were selectively labeled in vivo by incubating the cells with [3H]leucine and cycloheximide. When the labeled cells were washed free of...

Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps.

Gasser, S M, Ohashi, A, Daum, G, Böhni, P C, Gibson, J, Reid, G A, ...

Cytochrome b2 of yeast is located in the space between the inner and outer mitochondrial membranes whereas cytochrome c1 is bound to the outer face of the mitochondrial inner membrane. Both proteins...

A two-subunit cytochrome c oxidase (cytochrome aa3) from Paracoccus dentrificans.

Ludwig, B, Schatz, G

Cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, EC 1.9.3.1) was purified from the cytoplasmic membrane of the bacterium Paracoccus denitrificans. The enzyme contains two heme groups...

Cytoplasmically made subunits of yeast mitochondrial F1-ATPase and cytochrome c oxidase are synthesized as individual precursors, not as polyproteins.

Lewin, A S, Gregor, I, Mason, T L, Nelson, N, Schatz, G

At least three subunits of yeast mitochondrial F1-ATPase (ATP phosphohydrolase, EC 3.6.1.3) and at least two subunits of cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) are...

Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.

Maccecchini, M L, Rudin, Y, Blobel, G, Schatz, G

The mitochondrial F1-ATPase consists of five nonidentical subunits that are synthesized outside the mitochondria and imported across both mitochondrial membranes to the matrix side of the inner...

Artificial mitochondrial presequences.

Allison, D S, Schatz, G

Synthetic oligonucleotides were used to construct artificial mitochondrial presequences that contained, besides the initiator methionine, only arginine, serine, and leucine. The ratio of these three...

Promitochondria of Anaerobically Grown Yeast, V. Energy Transfer in the Absence of an Electron Transfer Chain

Groot, G. S. P., Kováč, L., Schatz, G.

Promitochondria of anaerobically grown Saccharomyces cerevisiae lack cytochromes aa3, b, c1, and c, as well as ubiquinone, yet catalyze a Pi-ATP exchange reaction that is sensitive to uncouplers,...

Two nuclear mutations that block mitochondrial protein import in yeast.

Yaffe, M P, Schatz, G

We isolated two yeast mutants that are temperature-sensitive for import of mitochondrial proteins. Each strain contains a single mutation that results in arrest of growth and accumulation of...

Acidic receptor domains on both sides of the outer membrane mediate translocation of precursor proteins into yeast mitochondria.

Bolliger, L, Junne, T, Schatz, G, Lithgow, T

Mitochondrial precursor proteins made in the cytosol bind to a hetero-oligomeric protein import receptor on the mitochondrial surface and then pass through the translocation channel across the outer...

A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability.

Bolliger, L, Deloche, O, Glick, B S, Georgopoulos, C, Jenö, P, Kronidou, N, ...

Mitochondrial hsp70 (mhsp70) is located in the matrix and an essential component of the mitochondrial protein import system. To study the function of mhsp70 and to identify possible partner proteins...

An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast.

Leighton, J, Schatz, G

In an attempt to identify a mitochondrial ATP binding cassette (ABC) transporter, we have used the polymerase chain reaction to amplify 10 DNA fragments homologous to members of the ABC family from...

Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria.

Horst, M, Hilfiker-Rothenfluh, S, Oppliger, W, Schatz, G

Mitochondria contain two distinct protein import systems, one in the outer and the other in the inner membrane. These systems can act independently of one another in submitochondrial fractions of if...

Energy-dependent processing of cytoplasmically made precursors to mitochondrial proteins.

Nelson, N, Schatz, G

Earlier work has shown that mitochondrial proteins synthesized in the cytosol are initially made as larger precursors which are then transferred into the organelles and processed to their mature size...

The signal that sorts yeast cytochrome b2 to the mitochondrial intermembrane space contains three distinct functional regions.

Beasley, E M, Müller, S, Schatz, G

Cytochrome b2, a protein of the yeast mitochondrial intermembrane space, is synthesized with an 80 residue bipartite presequence. The amino-terminal portion resembles a matrix-targeting signal. The...

Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product.

Horst, M, Jenö, P, Kronidou, N G, Bolliger, L, Oppliger, W, Scherer, P, ...

Protein import across both mitochondrial membranes is mediated by the cooperation of two distinct protein transport systems, one in the outer and the other in the inner membrane. Previously we...

Functional cooperation of mitochondrial protein import receptors in yeast.

Ramage, L, Junne, T, Hahne, K, Lithgow, T, Schatz, G

We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor. We cloned, sequenced and physically mapped the MAS20 gene...

Interaction of integral and peripheral membrane proteins: affinity labeling of yeast cytochrome oxidase by modified yeast cytochrome c.

Birchmeier, W, Kohler, C E, Schatz, G

To identify possible substrate-binding subunit(s) of yeast cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1-9-3-1), the purified enzyme was reacted with yeast iso-1-cytochrome c...

The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor.

Haucke, V, Horst, M, Schatz, G, Lithgow, T

Protein import into yeast mitochondria is mediated by four integral outer membrane proteins which function as import receptors. These proteins (termed Mas20p, Mas22p, Mas37p and Mas70p) appear to...

Hsp60-independent protein folding in the matrix of yeast mitochondria.

Rospert, S, Looser, R, Dubaquie, Y, Matouschek, A, Glick, B S, Schatz, G

Proteins that are imported from the cytosol into mitochondria cross the mitochondrial membranes in an unfolded conformation and then fold in the matrix. Some of these proteins require the chaperonin...

Inner membrane protease I, an enzyme mediating intramitochondrial protein sorting in yeast.

Schneider, A, Behrens, M, Scherer, P, Pratje, E, Michaelis, G, Schatz, G

Several precursors transported from the cytoplasm to the intermembrane space of yeast mitochondria are first cleaved by the MAS-encoded protease in the matrix space and then by additional proteases...

Sequential action of mitochondrial chaperones in protein import into the matrix.

Manning-Krieg, U C, Scherer, P E, Schatz, G

Translocation and folding of proteins imported into mitochondria are mediated by two matrix-localized chaperones, mhsp70 and hsp60. In order to investigate whether these chaperones act sequentially...

Amphiphilicity is essential for mitochondrial presequence function.

Roise, D, Theiler, F, Horvath, S J, Tomich, J M, Richards, J H, Allison, D S, ...

We have shown earlier that a mitochondrial presequence peptide can form an amphiphilic helix. However, the importance of amphiphilicity for mitochondrial presequence function became doubtful when an...

Unfolding and refolding of a purified precursor protein during import into isolated mitochondria.

Eilers, M, Hwang, S, Schatz, G

A purified mitochondrial precursor protein unfolds to a protease-sensitive conformation at the surface of isolated mitochondria before being imported into the organelles. This unfolding is stimulated...

Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria.

Vestweber, D, Schatz, G

In order to study the role of protein unfolding during post-translational protein import into mitochondria, we destabilized the structure of a mitochondrial precursor protein by site-directed...

Latent membrane perturbation activity of a mitochondrial precursor protein is exposed by unfolding.

Endo, T, Schatz, G

We have purified milligram amounts of an importable mitochondrial precursor protein [the presequence of yeast cytochrome oxidase subunit IV fused to mouse dihydrofolate reductase (DHFR)]. This has...

Import of proteins into yeast mitochondria: the purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes.

Yang, M, Jensen, R E, Yaffe, M P, Oppliger, W, Schatz, G

We have purified the metalloprotease which is localized in the soluble matrix space of Saccharomyces cerevisiae mitochondria and cleaves the amino-terminal matrix-targeting sequences from imported...

MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease.

Witte, C, Jensen, R E, Yaffe, M P, Schatz, G

We have previously described a yeast mutant (mas1) that accumulates mitochondrial precursor proteins at high temperature and is deficient in the activity of a matrix-localized protease which cleaves...

Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70.

Hines, V, Brandt, A, Griffiths, G, Horstmann, H, Brütsch, H, Schatz, G

The yeast mitochondrial outer membrane contains a major 70 kd protein with an amino-terminal hydrophobic membrane anchor and a hydrophilic 60 kd domain exposed to the cytosol. We now show that this...

A precursor protein partly translocated into yeast mitochondria is bound to a 70 kd mitochondrial stress protein.

Scherer, P E, Krieg, U C, Hwang, S T, Vestweber, D, Schatz, G

We have probed the environment of a precursor protein stuck in mitochondrial import sites using cleavable bifunctional crosslinking reagents. The stuck precursor was crosslinked to a 70 kd protein...

A yeast mutant lacking mitochondrial porin is respiratory-deficient, but can recover respiration with simultaneous accumulation of an 86-kd extramitochondrial protein.

Dihanich, M, Suda, K, Schatz, G

A yeast mutant lacking the only known pore-forming protein of the mitochondrial outer membrane was constructed by gene disruption. The mutant retained all other major proteins of the mitochondrial...

Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria.

Eilers, M, Oppliger, W, Schatz, G

We have investigated the energy requirement of mitochondrial protein import with a simplified system containing only isolated yeast mitochondria, energy sources and a purified precursor protein. This...

Protein import into yeast mitochondria is inhibited by antibodies raised against 45-kd proteins of the outer membrane.

Ohba, M, Schatz, G

Import of several precursor proteins into isolated yeast mitochondria is inhibited by rabbit antiserum raised against the total mitochondrial outer membrane or against electrophoretically purified...

Disruption of the outer membrane restores protein import to trypsin-treated yeast mitochondria.

Ohba, M, Schatz, G

Treatment of isolated yeast mitochondria with high levels (1 mg/ml) of trypsin severely inhibits protein import but does not destroy the integrity of the outer membrane or abolish mitochondrial...

Transport of proteins to the mitochondrial intermembrane space: the 'matrix-targeting' and the 'sorting' domains in the cytochrome c1 presequence.

Van Loon, A P, Brändli, A W, Pesold-Hurt, B, Blank, D, Schatz, G

We reported earlier that the yeast cytochrome c1 presequence (length: 61 amino acids) directs attached proteins to the mitochondrial intermembrane space and that it appears to contain two functional...

Transport of proteins to the mitochondrial intermembrane space: the 'sorting' domain of the cytochrome c1 presequence is a stop-transfer sequence specific for the mitochondrial inner membrane.

Van Loon, A P, Schatz, G

The presequence of yeast cytochrome c1 (an inner membrane protein protruding into the intermembrane space) contains a matrix-targeting domain and an intramitochondrial sorting domain. This...

Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.

Verner, K, Schatz, G

We have studied the post-translational import of incomplete precursor chains into isolated yeast mitochondria. The precursor was a fusion protein containing a mitochondrial presequence attached to...

The cytoplasmically-made subunit IV is necessary for assembly of cytochrome c oxidase in yeast.

Dowhan, W, Bibus, C R, Schatz, G

Yeast cytochrome c oxidase contains three large subunits made in mitochondria and at least six smaller subunits made in the cytoplasm. There is evidence that the catalytic centers (heme a and copper)...

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

Hurt, E C, Pesold-Hurt, B, Suda, K, Oppliger, W, Schatz, G

Yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) is made as a larger precursor with a transient pre-sequence of 25 amino acids. If this pre-sequence is fused to the amino...

A yeast mutant temperature-sensitive for mitochondrial assembly is deficient in a mitochondrial protease activity that cleaves imported precursor polypeptides.

Yaffe, M P, Ohta, S, Schatz, G

We have previously described two yeast mutants which, at elevated temperature, stop growing and accumulate precursors to several imported mitochondrial proteins. We now show that one of these mutants...

The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane.

Hurt, E C, Müller, U, Schatz, G

We have used an in vivo complementation assay to test whether a given polypeptide sequence can direct an attached protein to the mitochondrial inner membrane. The host is a previously described yeast...

Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane.

Riezman, H, Hase, T, Van Loon, A P, Grivell, L A, Suda, K, Schatz, G

The yeast mitochondrial outer membrane contains a major 70-kd protein which is coded by a nuclear gene. Two forms of this gene were isolated from a yeast genomic clone bank: the intact gene, and a...

Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane.

Hase, T, Riezman, H, Suda, K, Schatz, G

The nucleotide sequence of the yeast chromosomal gene coding for the 70-kd protein of the mitochondrial outer membrane was determined. The deduced amino acid sequence of the protein agrees with the...

Role of ATP in the intramitochondrial sorting of cytochrome c1 and the adenine nucleotide translocator.

Wachter, C, Schatz, G, Glick, B S

Import of precursor proteins across the mitochondrial inner membrane requires ATP in the matrix. However, some precursors can still cross the outer membrane in ATP-depleted mitochondria. Here we show...

A purified precursor polypeptide requires a cytosolic protein fraction for import into mitochondria.

Ohta, S, Schatz, G

The beta-subunit of mitochondrial ATPase is coded by a nuclear gene, synthesized outside the mitochondria as a larger precursor and imported into mitochondria. The beta-subunit precursor was purified...

Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.

Suissa, M, Suda, K, Schatz, G

To isolate nuclear genes specifying imported mitochondrial proteins, a yeast genomic clone bank was screened by an RNA hybridization-competition assay. This assay exploited the fact that mRNAs for...

Sequencing of the nuclear gene for the yeast cytochrome c1 precursor reveals an unusually complex amino-terminal presequence.

Sadler, I, Suda, K, Schatz, G, Kaudewitz, F, Haid, A

Cytochrome c1 is a component of the mitochondrial respiratory chain in most eukaryotes. The protein is coded by nuclear DNA, synthesized as a larger precursor outside the mitochondria and then...

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

Maarse, A C, Van Loon, A P, Riezman, H, Gregor, I, Schatz, G, Grivell, L A

The six small subunits (IV-VII, VIIa, VIII) of yeast cytochrome c oxidase are encoded by nuclear genes and imported into the mitochondria. We have isolated the gene for subunit IV from a yeast...

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

Hurt, E C, Pesold-Hurt, B, Schatz, G

Subunit IV of yeast cytochrome c oxidase is encoded by a nuclear gene, synthesized in the cytosol as a precursor with a transient amino-terminal extension of 25 amino acids, and imported into the...

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

Hase, T, Müller, U, Riezman, H, Schatz, G

The major 70-kd protein of the yeast mitochondrial outer membrane is made on cytosolic ribosomes and imported into the outer membrane without proteolytic cleavage. We have attempted to identify the...

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

Roise, D, Horvath, S J, Tomich, J M, Richards, J H, Schatz, G

Subunit IV of yeast cytochrome oxidase is made in the cytoplasm with a transient pre-sequence of 25 amino acids which is removed upon import of the protein into mitochondria. To study the function of...

Sequential action of two hsp70 complexes during protein import into mitochondria.

Horst, M, Oppliger, W, Rospert, S, Schönfeld, H J, Schatz, G, Azem, A

The mitochondrial chaperone mhsp70 mediates protein transport across the inner membrane and protein folding in the matrix. These two reactions are effected by two different mhsp70 complexes. The ADP...

Binding of mitochondrial precursor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones.

Komiya, T, Rospert, S, Schatz, G, Mihara, K

We have reconstituted the early steps of precursor targeting to mitochondria in a defined and soluble system consisting of the cytosolic domains of the yeast mitochondrial import receptors Tom20 and...

Reconstitution of the protein insertion machinery of the mitochondrial inner membrane.

Haucke, V, Schatz, G

We have reconstituted the protein insertion machinery of the yeast mitochondrial inner membrane into proteoliposomes. The reconstituted proteoliposomes have a distinct morphology and protein...

Active unfolding of precursor proteins during mitochondrial protein import.

Matouschek, A, Azem, A, Ratliff, K, Glick, B S, Schmid, K, Schatz, G

Precursor proteins made in the cytoplasm must be in an unfolded conformation during import into mitochondria. Some precursor proteins have tightly folded domains but are imported faster than they...

Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway: evidence for the 'acid chain' hypothesis.

Komiya, T, Rospert, S, Koehler, C, Looser, R, Schatz, G, Mihara, K

Mitochondrial precursor proteins with basic targeting signals may be transported across the outer membrane by sequential binding to acidic receptor sites of increasing affinity. To test this 'acid...

Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins.

Koehler, C M, Merchant, S, Oppliger, W, Schmid, K, Jarosch, E, Dolfini, L, ...

Tim10p, a protein of the yeast mitochondrial intermembrane space, was shown previously to be essential for the import of multispanning carrier proteins from the cytoplasm into the inner membrane. We...

Different import pathways through the mitochondrial intermembrane space for inner membrane proteins.

Leuenberger, D, Bally, N A, Schatz, G, Koehler, C M

Earlier work on the protein import system of yeast mitochondria has identified two soluble 70 kDa protein complexes in the intermembrane space. One complex contains the essential proteins Tim9p and...

Cyclophilin catalyzes protein folding in yeast mitochondria.

Matouschek, A, Rospert, S, Schmid, K, Glick, B S, Schatz, G

Cyclophilins are a family of ubiquitous proteins that are the intracellular target of the immunosuppressant drug cyclosporin A. Although cyclophilins catalyze peptidylprolyl cis-trans isomerization...

The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast.

Lithgow, T, Junne, T, Suda, K, Gratzer, S, Schatz, G

We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses...

Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.

Kronidou, N G, Oppliger, W, Bolliger, L, Hannavy, K, Glick, B S, Schatz, G, ...

The protein import system of the yeast mitochondrial inner membrane includes at least three membrane proteins that presumably form a transmembrane channel as well as several chaperone proteins that...

Identification and functional analysis of chaperonin 10, the groES homolog from yeast mitochondria.

Rospert, S, Glick, B S, Jenö, P, Schatz, G, Todd, M J, Lorimer, G H, ...

Chaperonin 60 (cpn60) and chaperonin 10 (cpn10) constitute the chaperonin system in prokaryotes, mitochondria, and chloroplasts. In Escherichia coli, these two chaperonins are also termed groEL and...

Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane.

Scherer, P E, Manning-Krieg, U C, Jenö, P, Schatz, G, Horst, M

Import of proteins into mitochondria involves the cooperation of protein translocation systems in the outer and inner membranes. We have identified a 45-kDa protein at the protein import site of the...

Analysis of cleavage site mutations between the NC and PR Gag domains of Rous sarcoma virus.

Schatz, G, Pichova, I, Vogt, V M

In retroviruses, the viral protease (PR) is released as a mature protein by cleavage of Gag, Gag-Pro, or Gag-Pro-Pol precursor polypeptides. In avian sarcoma and leukemia viruses (ASLV), PR forms the...

Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.

Haucke, V, Ocana, C S, Hönlinger, A, Tokatlidis, K, Pfanner, N, Schatz, G

Mitochondrial NADH-cytochrome b5 reductase (Mcr1p) is encoded by a single nuclear gene and imported into two different submitochondrial compartments: the outer membrane and the intermembrane space....

Processing of avian retroviral gag polyprotein precursors is blocked by a mutation at the NC-PR cleavage site.

Burstein, H, Bizub, D, Kotler, M, Schatz, G, Vogt, V M, Skalka, A M

The avian sarcoma and leukosis viruses (ASLV) encode a protease (PR) at the C terminus of gag which in vivo catalyzes the processing of both gag and gag-pol precursors. The studies reported here were...

Properties of avian retrovirus particles defective in viral protease.

Stewart, L, Schatz, G, Vogt, V M

The structural and enzymatic components of retroviral cores are formed by proteolytic cleavage of precursor polypeptides, mediated by the viral protease (PR). We constructed an active-site mutation,...

Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.

Hwang, S T, Schatz, G

Work in several laboratories has established that import of proteins across the mitochondrial inner membrane requires an electrochemical potential across that membrane and cleavage of nucleoside...

Protein import into mitochondria: the requirement for external ATP is precursor-specific whereas intramitochondrial ATP is universally needed for translocation into the matrix.

Wachter, C, Schatz, G, Glick, B S

ATP is needed for the import of precursor proteins into mitochondria. However, the role of ATP and its site of action have been unclear. We have now investigated the ATP requirements for protein...

Sequences from a prokaryotic genome or the mouse dihydrofolate reductase gene can restore the import of a truncated precursor protein into yeast mitochondria.

Baker, A, Schatz, G

Sequences that are capable of restoring mitochondrial targeting to a truncated yeast cytochrome c oxidase subunit IV presequence are encoded within the genome of Escherichia coli and within the gene...

A yeast mutant lacking mitochondrial manganese-superoxide dismutase is hypersensitive to oxygen.

Van Loon, A P, Pesold-Hurt, B, Schatz, G

The nuclear gene for manganese-containing superoxide dismutase (MnSOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) of yeast mitochondria was mapped on chromosome VIII and inactivated by gene...

PROMITOCHONDRIA OF ANAEROBICALLY GROWN YEAST, IV. CONVERSION INTO RESPIRING MITOCHONDRIA*

Plattner, H., Salpeter, M. M., Saltzgaber, J., Schatz, G.

Promitochondria of anaerobically grown Saccharomyces cerevisiae were selectively labeled in vivo by incubating the cells with [3H]leucine and cycloheximide. When the labeled cells were washed free of...

Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps.

Gasser, S M, Ohashi, A, Daum, G, Böhni, P C, Gibson, J, Reid, G A, ...

Cytochrome b2 of yeast is located in the space between the inner and outer mitochondrial membranes whereas cytochrome c1 is bound to the outer face of the mitochondrial inner membrane. Both proteins...

A two-subunit cytochrome c oxidase (cytochrome aa3) from Paracoccus dentrificans.

Ludwig, B, Schatz, G

Cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, EC 1.9.3.1) was purified from the cytoplasmic membrane of the bacterium Paracoccus denitrificans. The enzyme contains two heme groups...

Cytoplasmically made subunits of yeast mitochondrial F1-ATPase and cytochrome c oxidase are synthesized as individual precursors, not as polyproteins.

Lewin, A S, Gregor, I, Mason, T L, Nelson, N, Schatz, G

At least three subunits of yeast mitochondrial F1-ATPase (ATP phosphohydrolase, EC 3.6.1.3) and at least two subunits of cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) are...

Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.

Maccecchini, M L, Rudin, Y, Blobel, G, Schatz, G

The mitochondrial F1-ATPase consists of five nonidentical subunits that are synthesized outside the mitochondria and imported across both mitochondrial membranes to the matrix side of the inner...

Artificial mitochondrial presequences.

Allison, D S, Schatz, G

Synthetic oligonucleotides were used to construct artificial mitochondrial presequences that contained, besides the initiator methionine, only arginine, serine, and leucine. The ratio of these three...

Promitochondria of Anaerobically Grown Yeast, V. Energy Transfer in the Absence of an Electron Transfer Chain

Groot, G. S. P., Kováč, L., Schatz, G.

Promitochondria of anaerobically grown Saccharomyces cerevisiae lack cytochromes aa3, b, c1, and c, as well as ubiquinone, yet catalyze a Pi-ATP exchange reaction that is sensitive to uncouplers,...

Two nuclear mutations that block mitochondrial protein import in yeast.

Yaffe, M P, Schatz, G

We isolated two yeast mutants that are temperature-sensitive for import of mitochondrial proteins. Each strain contains a single mutation that results in arrest of growth and accumulation of...

Acidic receptor domains on both sides of the outer membrane mediate translocation of precursor proteins into yeast mitochondria.

Bolliger, L, Junne, T, Schatz, G, Lithgow, T

Mitochondrial precursor proteins made in the cytosol bind to a hetero-oligomeric protein import receptor on the mitochondrial surface and then pass through the translocation channel across the outer...

A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability.

Bolliger, L, Deloche, O, Glick, B S, Georgopoulos, C, Jenö, P, Kronidou, N, ...

Mitochondrial hsp70 (mhsp70) is located in the matrix and an essential component of the mitochondrial protein import system. To study the function of mhsp70 and to identify possible partner proteins...

An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast.

Leighton, J, Schatz, G

In an attempt to identify a mitochondrial ATP binding cassette (ABC) transporter, we have used the polymerase chain reaction to amplify 10 DNA fragments homologous to members of the ABC family from...

Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria.

Horst, M, Hilfiker-Rothenfluh, S, Oppliger, W, Schatz, G

Mitochondria contain two distinct protein import systems, one in the outer and the other in the inner membrane. These systems can act independently of one another in submitochondrial fractions of if...

Energy-dependent processing of cytoplasmically made precursors to mitochondrial proteins.

Nelson, N, Schatz, G

Earlier work has shown that mitochondrial proteins synthesized in the cytosol are initially made as larger precursors which are then transferred into the organelles and processed to their mature size...

The signal that sorts yeast cytochrome b2 to the mitochondrial intermembrane space contains three distinct functional regions.

Beasley, E M, Müller, S, Schatz, G

Cytochrome b2, a protein of the yeast mitochondrial intermembrane space, is synthesized with an 80 residue bipartite presequence. The amino-terminal portion resembles a matrix-targeting signal. The...

Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product.

Horst, M, Jenö, P, Kronidou, N G, Bolliger, L, Oppliger, W, Scherer, P, ...

Protein import across both mitochondrial membranes is mediated by the cooperation of two distinct protein transport systems, one in the outer and the other in the inner membrane. Previously we...

Functional cooperation of mitochondrial protein import receptors in yeast.

Ramage, L, Junne, T, Hahne, K, Lithgow, T, Schatz, G

We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor. We cloned, sequenced and physically mapped the MAS20 gene...

Interaction of integral and peripheral membrane proteins: affinity labeling of yeast cytochrome oxidase by modified yeast cytochrome c.

Birchmeier, W, Kohler, C E, Schatz, G

To identify possible substrate-binding subunit(s) of yeast cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1-9-3-1), the purified enzyme was reacted with yeast iso-1-cytochrome c...

The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor.

Haucke, V, Horst, M, Schatz, G, Lithgow, T

Protein import into yeast mitochondria is mediated by four integral outer membrane proteins which function as import receptors. These proteins (termed Mas20p, Mas22p, Mas37p and Mas70p) appear to...

Hsp60-independent protein folding in the matrix of yeast mitochondria.

Rospert, S, Looser, R, Dubaquie, Y, Matouschek, A, Glick, B S, Schatz, G

Proteins that are imported from the cytosol into mitochondria cross the mitochondrial membranes in an unfolded conformation and then fold in the matrix. Some of these proteins require the chaperonin...

Inner membrane protease I, an enzyme mediating intramitochondrial protein sorting in yeast.

Schneider, A, Behrens, M, Scherer, P, Pratje, E, Michaelis, G, Schatz, G

Several precursors transported from the cytoplasm to the intermembrane space of yeast mitochondria are first cleaved by the MAS-encoded protease in the matrix space and then by additional proteases...

Sequential action of mitochondrial chaperones in protein import into the matrix.

Manning-Krieg, U C, Scherer, P E, Schatz, G

Translocation and folding of proteins imported into mitochondria are mediated by two matrix-localized chaperones, mhsp70 and hsp60. In order to investigate whether these chaperones act sequentially...

Amphiphilicity is essential for mitochondrial presequence function.

Roise, D, Theiler, F, Horvath, S J, Tomich, J M, Richards, J H, Allison, D S, ...

We have shown earlier that a mitochondrial presequence peptide can form an amphiphilic helix. However, the importance of amphiphilicity for mitochondrial presequence function became doubtful when an...

Unfolding and refolding of a purified precursor protein during import into isolated mitochondria.

Eilers, M, Hwang, S, Schatz, G

A purified mitochondrial precursor protein unfolds to a protease-sensitive conformation at the surface of isolated mitochondria before being imported into the organelles. This unfolding is stimulated...

Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria.

Vestweber, D, Schatz, G

In order to study the role of protein unfolding during post-translational protein import into mitochondria, we destabilized the structure of a mitochondrial precursor protein by site-directed...

Latent membrane perturbation activity of a mitochondrial precursor protein is exposed by unfolding.

Endo, T, Schatz, G

We have purified milligram amounts of an importable mitochondrial precursor protein [the presequence of yeast cytochrome oxidase subunit IV fused to mouse dihydrofolate reductase (DHFR)]. This has...

Import of proteins into yeast mitochondria: the purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes.

Yang, M, Jensen, R E, Yaffe, M P, Oppliger, W, Schatz, G

We have purified the metalloprotease which is localized in the soluble matrix space of Saccharomyces cerevisiae mitochondria and cleaves the amino-terminal matrix-targeting sequences from imported...

MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease.

Witte, C, Jensen, R E, Yaffe, M P, Schatz, G

We have previously described a yeast mutant (mas1) that accumulates mitochondrial precursor proteins at high temperature and is deficient in the activity of a matrix-localized protease which cleaves...

Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70.

Hines, V, Brandt, A, Griffiths, G, Horstmann, H, Brütsch, H, Schatz, G

The yeast mitochondrial outer membrane contains a major 70 kd protein with an amino-terminal hydrophobic membrane anchor and a hydrophilic 60 kd domain exposed to the cytosol. We now show that this...

A precursor protein partly translocated into yeast mitochondria is bound to a 70 kd mitochondrial stress protein.

Scherer, P E, Krieg, U C, Hwang, S T, Vestweber, D, Schatz, G

We have probed the environment of a precursor protein stuck in mitochondrial import sites using cleavable bifunctional crosslinking reagents. The stuck precursor was crosslinked to a 70 kd protein...

A yeast mutant lacking mitochondrial porin is respiratory-deficient, but can recover respiration with simultaneous accumulation of an 86-kd extramitochondrial protein.

Dihanich, M, Suda, K, Schatz, G

A yeast mutant lacking the only known pore-forming protein of the mitochondrial outer membrane was constructed by gene disruption. The mutant retained all other major proteins of the mitochondrial...

Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria.

Eilers, M, Oppliger, W, Schatz, G

We have investigated the energy requirement of mitochondrial protein import with a simplified system containing only isolated yeast mitochondria, energy sources and a purified precursor protein. This...

Protein import into yeast mitochondria is inhibited by antibodies raised against 45-kd proteins of the outer membrane.

Ohba, M, Schatz, G

Import of several precursor proteins into isolated yeast mitochondria is inhibited by rabbit antiserum raised against the total mitochondrial outer membrane or against electrophoretically purified...

Disruption of the outer membrane restores protein import to trypsin-treated yeast mitochondria.

Ohba, M, Schatz, G

Treatment of isolated yeast mitochondria with high levels (1 mg/ml) of trypsin severely inhibits protein import but does not destroy the integrity of the outer membrane or abolish mitochondrial...

Transport of proteins to the mitochondrial intermembrane space: the 'matrix-targeting' and the 'sorting' domains in the cytochrome c1 presequence.

Van Loon, A P, Brändli, A W, Pesold-Hurt, B, Blank, D, Schatz, G

We reported earlier that the yeast cytochrome c1 presequence (length: 61 amino acids) directs attached proteins to the mitochondrial intermembrane space and that it appears to contain two functional...

Transport of proteins to the mitochondrial intermembrane space: the 'sorting' domain of the cytochrome c1 presequence is a stop-transfer sequence specific for the mitochondrial inner membrane.

Van Loon, A P, Schatz, G

The presequence of yeast cytochrome c1 (an inner membrane protein protruding into the intermembrane space) contains a matrix-targeting domain and an intramitochondrial sorting domain. This...

Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.

Verner, K, Schatz, G

We have studied the post-translational import of incomplete precursor chains into isolated yeast mitochondria. The precursor was a fusion protein containing a mitochondrial presequence attached to...

The cytoplasmically-made subunit IV is necessary for assembly of cytochrome c oxidase in yeast.

Dowhan, W, Bibus, C R, Schatz, G

Yeast cytochrome c oxidase contains three large subunits made in mitochondria and at least six smaller subunits made in the cytoplasm. There is evidence that the catalytic centers (heme a and copper)...

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

Hurt, E C, Pesold-Hurt, B, Suda, K, Oppliger, W, Schatz, G

Yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) is made as a larger precursor with a transient pre-sequence of 25 amino acids. If this pre-sequence is fused to the amino...

A yeast mutant temperature-sensitive for mitochondrial assembly is deficient in a mitochondrial protease activity that cleaves imported precursor polypeptides.

Yaffe, M P, Ohta, S, Schatz, G

We have previously described two yeast mutants which, at elevated temperature, stop growing and accumulate precursors to several imported mitochondrial proteins. We now show that one of these mutants...

The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane.

Hurt, E C, Müller, U, Schatz, G

We have used an in vivo complementation assay to test whether a given polypeptide sequence can direct an attached protein to the mitochondrial inner membrane. The host is a previously described yeast...

Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane.

Riezman, H, Hase, T, Van Loon, A P, Grivell, L A, Suda, K, Schatz, G

The yeast mitochondrial outer membrane contains a major 70-kd protein which is coded by a nuclear gene. Two forms of this gene were isolated from a yeast genomic clone bank: the intact gene, and a...

Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane.

Hase, T, Riezman, H, Suda, K, Schatz, G

The nucleotide sequence of the yeast chromosomal gene coding for the 70-kd protein of the mitochondrial outer membrane was determined. The deduced amino acid sequence of the protein agrees with the...

Role of ATP in the intramitochondrial sorting of cytochrome c1 and the adenine nucleotide translocator.

Wachter, C, Schatz, G, Glick, B S

Import of precursor proteins across the mitochondrial inner membrane requires ATP in the matrix. However, some precursors can still cross the outer membrane in ATP-depleted mitochondria. Here we show...

A purified precursor polypeptide requires a cytosolic protein fraction for import into mitochondria.

Ohta, S, Schatz, G

The beta-subunit of mitochondrial ATPase is coded by a nuclear gene, synthesized outside the mitochondria as a larger precursor and imported into mitochondria. The beta-subunit precursor was purified...

Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.

Suissa, M, Suda, K, Schatz, G

To isolate nuclear genes specifying imported mitochondrial proteins, a yeast genomic clone bank was screened by an RNA hybridization-competition assay. This assay exploited the fact that mRNAs for...

Sequencing of the nuclear gene for the yeast cytochrome c1 precursor reveals an unusually complex amino-terminal presequence.

Sadler, I, Suda, K, Schatz, G, Kaudewitz, F, Haid, A

Cytochrome c1 is a component of the mitochondrial respiratory chain in most eukaryotes. The protein is coded by nuclear DNA, synthesized as a larger precursor outside the mitochondria and then...

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

Maarse, A C, Van Loon, A P, Riezman, H, Gregor, I, Schatz, G, Grivell, L A

The six small subunits (IV-VII, VIIa, VIII) of yeast cytochrome c oxidase are encoded by nuclear genes and imported into the mitochondria. We have isolated the gene for subunit IV from a yeast...

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

Hurt, E C, Pesold-Hurt, B, Schatz, G

Subunit IV of yeast cytochrome c oxidase is encoded by a nuclear gene, synthesized in the cytosol as a precursor with a transient amino-terminal extension of 25 amino acids, and imported into the...

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

Hase, T, Müller, U, Riezman, H, Schatz, G

The major 70-kd protein of the yeast mitochondrial outer membrane is made on cytosolic ribosomes and imported into the outer membrane without proteolytic cleavage. We have attempted to identify the...

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

Roise, D, Horvath, S J, Tomich, J M, Richards, J H, Schatz, G

Subunit IV of yeast cytochrome oxidase is made in the cytoplasm with a transient pre-sequence of 25 amino acids which is removed upon import of the protein into mitochondria. To study the function of...

Sequential action of two hsp70 complexes during protein import into mitochondria.

Horst, M, Oppliger, W, Rospert, S, Schönfeld, H J, Schatz, G, Azem, A

The mitochondrial chaperone mhsp70 mediates protein transport across the inner membrane and protein folding in the matrix. These two reactions are effected by two different mhsp70 complexes. The ADP...

Binding of mitochondrial precursor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones.

Komiya, T, Rospert, S, Schatz, G, Mihara, K

We have reconstituted the early steps of precursor targeting to mitochondria in a defined and soluble system consisting of the cytosolic domains of the yeast mitochondrial import receptors Tom20 and...

Reconstitution of the protein insertion machinery of the mitochondrial inner membrane.

Haucke, V, Schatz, G

We have reconstituted the protein insertion machinery of the yeast mitochondrial inner membrane into proteoliposomes. The reconstituted proteoliposomes have a distinct morphology and protein...

Active unfolding of precursor proteins during mitochondrial protein import.

Matouschek, A, Azem, A, Ratliff, K, Glick, B S, Schmid, K, Schatz, G

Precursor proteins made in the cytoplasm must be in an unfolded conformation during import into mitochondria. Some precursor proteins have tightly folded domains but are imported faster than they...

Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway: evidence for the 'acid chain' hypothesis.

Komiya, T, Rospert, S, Koehler, C, Looser, R, Schatz, G, Mihara, K

Mitochondrial precursor proteins with basic targeting signals may be transported across the outer membrane by sequential binding to acidic receptor sites of increasing affinity. To test this 'acid...

Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins.

Koehler, C M, Merchant, S, Oppliger, W, Schmid, K, Jarosch, E, Dolfini, L, ...

Tim10p, a protein of the yeast mitochondrial intermembrane space, was shown previously to be essential for the import of multispanning carrier proteins from the cytoplasm into the inner membrane. We...

Different import pathways through the mitochondrial intermembrane space for inner membrane proteins.

Leuenberger, D, Bally, N A, Schatz, G, Koehler, C M

Earlier work on the protein import system of yeast mitochondria has identified two soluble 70 kDa protein complexes in the intermembrane space. One complex contains the essential proteins Tim9p and...

Import of cytochrome b2 to the mitochondrial intermembrane space: the tightly folded heme-binding domain makes import dependent upon matrix ATP.

Glick, B. S., Wachter, C., Reid, G. A., Schatz, G.

Cytochrome b2 is synthesized as a precursor in the cytoplasm and imported to the intermembrane space of yeast mitochondria. We show here that the precursor contains a tightly folded heme-binding...

The mitochondrial protein import motor: dissociation of mitochondrial hsp70 from its membrane anchor requires ATP binding rather than ATP hydrolysis.

Horst, M., Oppliger, W., Feifel, B., Schatz, G., Glick, B. S.

During protein import into mitochondria, matrix-localized mitochondrial hsp70 (mhsp70) interacts with the inner membrane protein Tim44 to pull a precursor across the inner membrane. We have proposed...