Siegel, T. Nicolai, Hekstra, Doeke R., Kemp, Louise E., Figueiredo, Luisa M., Lowell, Joanna E., Fenyo, David, ...
Unusually for a eukaryote, genes transcribed by RNA polymerase II (pol II) in Trypanosoma brucei are arranged in polycistronic transcription units. With one exception, no pol II promoter motifs have...
Telomere structure and shortening in telomerase-deficient Trypanosoma brucei (2005)
Dreesen, Oliver, Li, Bibo, Cross, George A. M.
Telomerase consists of a reverse transcriptase (TERT) and an RNA that contains a template for telomere-repeat extension. Telomerase is required to prevent telomere erosion and its activity or lack...
Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei (2004)
Janzen, Christian J., Lander, Fabian, Dreesen, Oliver, Cross, George A. M.
KU is a heterodimer, consisting of ∼70 and ∼80 kDa subunits (KU70 and KU80, respectively), which is involved in a variety of nuclear functions. We generated tbKU80-deficient trypanosomes to...
An organism-specific method to rank predicted coding regions in Trypanosoma brucei (2003)
Gopal, Shuba, Cross, George A. M., Gaasterland, Terry
Genome annotation in differently evolved organisms presents challenges because the lack of sequence‐based homology limits the ability to determine the function of putative coding regions. To...
Engstler, Markus, Wirtz, Elizabeth, Cross, George A.M.
Trans-sialylation is a unique enzymatic process that is restricted to some trypanosome species. By expressing developmentally regulated trans-sialidases, these protozoan parasites cleave sialic acids...
Haynes, Paul A., Ferguson, A.J., Cross, George A.M.
Affinity-purified glycopeptides were prepared from Trypanosoma cruzi using the carbohydrate-specific monoclonal antibody WIC29.26. These glycopeptides contain rhamnose, fucose, xylose, and galactose,...
Bellofatto, Vivian, Cooper, Robin, Cross, George A.M.
Mature mRNAs of trypanosomatid protozoa result from the joining of at least two exons, which are initially transcribed as separate RNAs. In all trypanosomatids examined to date, the first exon...
Freistadt, Marion S., Cross, George A.M., Branch, Andrea D., Robertson, Hugh D.
The mini-exon, a short segment found at the 5′ end of trypanosome mRNAs, is contributed by a small RNA, the mini-exon donor (medRNA). In vivo 32P-labeled medRNA, a set of smaller RNAs related to...
Boothroyd, John C., Paynter, Cora A., Cross, George A.M., Bernards, Andre, Borst, Piet
cDNAs coding for the amino and carboxy termini of two trypanosome variant surface glycoproteins (VSGs) have been sequenced. The results indicate that VSGs are synthesised with hydrophobic...
Purification, cloning and characterization of a GPI inositol deacylase from Trypanosoma brucei
Güther, Maria Lucia Sampaio, Leal, Simone, Morrice, Nicholas A., Cross, George A.M., Ferguson, Michael A.J.
Inositol acylation is an obligatory step in glycosylphosphatidylinositol (GPI) biosynthesis whereas mature GPI anchors often lack this modification. The GPI anchors of Trypanosoma brucei variant...
t-loops at trypanosome telomeres
Muñoz-Jordán, Jorge L., Cross, George A.M., Lange, Titia De, Griffith, Jack D.
Mammalian telomeres form large duplex loops (t-loops) that may sequester chromosome ends by invasion of the 3′ TTAGGG overhang into the duplex TTAGGG repeat array. Here we document t-loops in...
An organism-specific method to rank predicted coding regions in Trypanosoma brucei
Gopal, Shuba, Cross, George A. M., Gaasterland, Terry
Genome annotation in differently evolved organisms presents challenges because the lack of sequence-based homology limits the ability to determine the function of putative coding regions. To provide...
Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei
Janzen, Christian J., Lander, Fabian, Dreesen, Oliver, Cross, George A. M.
KU is a heterodimer, consisting of ∼70 and ∼80 kDa subunits (KU70 and KU80, respectively), which is involved in a variety of nuclear functions. We generated tbKU80-deficient trypanosomes to...
Trypanosome Telomeres Are Protected by a Homologue of Mammalian TRF2
Li, Bibo, Espinal, Amin, Cross, George A. M.
Putative TTAGGG repeat-binding factor (TRF) homologues in the genomes of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major were identified. They have significant sequence similarity to...
Telomere structure and shortening in telomerase-deficient Trypanosoma brucei
Dreesen, Oliver, Li, Bibo, Cross, George A. M.
Telomerase consists of a reverse transcriptase (TERT) and an RNA that contains a template for telomere-repeat extension. Telomerase is required to prevent telomere erosion and its activity or lack...
Das, Anish, Zhang, Qing, Palenchar, Jennifer B., Chatterjee, Bithi, Cross, George A. M., Bellofatto, Vivian
Protein-coding genes of trypanosomes are mainly transcribed polycistronically and cleaved into functional mRNAs in a process that requires trans splicing of a capped 39-nucleotide RNA derived from a...
Systematic Study of Sequence Motifs for RNA trans Splicing in Trypanosoma brucei
Siegel, T. Nicolai, Tan, Kevin S. W., Cross, George A. M.
mRNA maturation in Trypanosoma brucei depends upon trans splicing, and variations in trans-splicing efficiency could be an important step in controlling the levels of individual mRNAs. RNA splicing...
Telomerase-Independent Stabilization of Short Telomeres in Trypanosoma brucei
Dreesen, Oliver, Cross, George A. M.
In cancer cells and germ cells, shortening of chromosome ends is prevented by telomerase. Telomerase-deficient cells have a replicative life span, after which they enter senescence. Senescent cells...
Purification, cloning and characterization of a GPI inositol deacylase from Trypanosoma brucei
Güther, Maria Lucia Sampaio, Leal, Simone, Morrice, Nicholas A., Cross, George A.M., Ferguson, Michael A.J.
Inositol acylation is an obligatory step in glycosylphosphatidylinositol (GPI) biosynthesis whereas mature GPI anchors often lack this modification. The GPI anchors of Trypanosoma brucei variant...
t-loops at trypanosome telomeres
Muñoz-Jordán, Jorge L., Cross, George A.M., Lange, Titia De, Griffith, Jack D.
Mammalian telomeres form large duplex loops (t-loops) that may sequester chromosome ends by invasion of the 3′ TTAGGG overhang into the duplex TTAGGG repeat array. Here we document t-loops in...
An organism-specific method to rank predicted coding regions in Trypanosoma brucei
Gopal, Shuba, Cross, George A. M., Gaasterland, Terry
Genome annotation in differently evolved organisms presents challenges because the lack of sequence-based homology limits the ability to determine the function of putative coding regions. To provide...
Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei
Janzen, Christian J., Lander, Fabian, Dreesen, Oliver, Cross, George A. M.
KU is a heterodimer, consisting of ∼70 and ∼80 kDa subunits (KU70 and KU80, respectively), which is involved in a variety of nuclear functions. We generated tbKU80-deficient trypanosomes to...
Trypanosome Telomeres Are Protected by a Homologue of Mammalian TRF2
Li, Bibo, Espinal, Amin, Cross, George A. M.
Putative TTAGGG repeat-binding factor (TRF) homologues in the genomes of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major were identified. They have significant sequence similarity to...
Telomere structure and shortening in telomerase-deficient Trypanosoma brucei
Dreesen, Oliver, Li, Bibo, Cross, George A. M.
Telomerase consists of a reverse transcriptase (TERT) and an RNA that contains a template for telomere-repeat extension. Telomerase is required to prevent telomere erosion and its activity or lack...
Das, Anish, Zhang, Qing, Palenchar, Jennifer B., Chatterjee, Bithi, Cross, George A. M., Bellofatto, Vivian
Protein-coding genes of trypanosomes are mainly transcribed polycistronically and cleaved into functional mRNAs in a process that requires trans splicing of a capped 39-nucleotide RNA derived from a...
Systematic Study of Sequence Motifs for RNA trans Splicing in Trypanosoma brucei
Siegel, T. Nicolai, Tan, Kevin S. W., Cross, George A. M.
mRNA maturation in Trypanosoma brucei depends upon trans splicing, and variations in trans-splicing efficiency could be an important step in controlling the levels of individual mRNAs. RNA splicing...
Telomerase-Independent Stabilization of Short Telomeres in Trypanosoma brucei
Dreesen, Oliver, Cross, George A. M.
In cancer cells and germ cells, shortening of chromosome ends is prevented by telomerase. Telomerase-deficient cells have a replicative life span, after which they enter senescence. Senescent cells...
Dreesen, Oliver, Cross, George A. M.
Trypanosoma brucei evades the host immune response by sequential expression of a large family of variant surface glycoproteins (VSG) from one of ∼20 subtelomeric expression sites (ES). VSG...
An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA
Rubio, Mary Anne T., Pastar, Irena, Gaston, Kirk W., Ragone, Frank L., Janzen, Christian J., Cross, George A. M., ...
Adenosine-to-inosine editing in the anticodon of tRNAs is essential for viability. Enzymes mediating tRNA adenosine deamination in bacteria and yeast contain cytidine deaminase-conserved motifs,...
Acetylation of histone H4K4 is cell cycle regulated and mediated by HAT3 in Trypanosoma brucei
Siegel, T Nicolai, Kawahara, Taemi, DeGrasse, Jeffrey A, Janzen, Christian J, Horn, David, Cross, George A M
Post-translational histone modifications have been studied intensively in several eukaryotes. It has been proposed that these modifications constitute a ‘histone code’ that specifies epigenetic...
A Histone Methyltransferase Modulates Antigenic Variation in African Trypanosomes
Figueiredo, Luisa M, Janzen, Christian J, Cross, George A.M
To evade the host immune system, several pathogens periodically change their cell-surface epitopes. In the African trypanosomes, antigenic variation is achieved by tightly regulating the expression...
Telomeric Expression Sites Are Highly Conserved in Trypanosoma brucei
Hertz-Fowler, Christiane, Figueiredo, Luisa M., Quail, Michael A., Becker, Marion, Jackson, Andrew, Bason, Nathalie, ...
Subtelomeric regions are often under-represented in genome sequences of eukaryotes. One of the best known examples of the use of telomere proximity for adaptive purposes are the bloodstream...
Multifunctional class I transcription in Trypanosoma brucei depends on a novel protein complex
Brandenburg, Jens, Schimanski, Bernd, Nogoceke, Everson, Nguyen, Tu N, Padovan, Júlio C, Chait, Brian T, ...
The vector-borne, protistan parasite Trypanosoma brucei is the only known eukaryote with a multifunctional RNA polymerase I that, in addition to ribosomal genes, transcribes genes encoding the...
Kawahara, Taemi, Siegel, T Nicolai, Ingram, Alexandra K, Alsford, Sam, Cross, George A M, Horn, David
Chromatin modification is important for virtually all aspects of DNA metabolism but little is known about the consequences of such modification in trypanosomatids, early branching protozoa of...
Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
Siegel, T. Nicolai, Hekstra, Doeke R., Kemp, Louise E., Figueiredo, Luisa M., Lowell, Joanna E., Fenyo, David, ...
Unusually for a eukaryote, genes transcribed by RNA polymerase II (pol II) in Trypanosoma brucei are arranged in polycistronic transcription units. With one exception, no pol II promoter motifs have...