Affinities of ribosomal protein S20 and C-terminal deletion mutants for 16S rRNA and S20 mRNA (1988)
Donly, B.Cameron, Mackie, George A.
We have measured the binding of E. coli ribosomal protein S20 and a number of C-terminal deletion mutants to 16S rRNA and in vitro transcribed S20 mRNA. Mutant S20s of interest were synthesized in...
The sequence of nucleotides extending over 2.3 kb distal to the gene for ribosomal protein S20 of E. coli has been determined. Included in the sequence is an efficient rhoindependent terminator 50...
Primary sequence of the 16S ribosomal RNA of Escherichia coli (1975)
Ehresmann, Chantal, Stiegler, Patrick, Mackie, George A., Zimmermann, Robert A., Ebel, J.P., Fellner, Peter
Recent progress in the nucleotide sequence analysis of the 16S ribosomal RNA from E.coli is described. The sequence which has been partially or completely determined so far encompasses 1520...
Spickler, Catherine, Stronge, Victoria, Mackie, George A.
RNase E, the principal RNase capable of initiating mRNA decay, preferentially attacks 5′-monophosphorylated over 5′-triphosphorylated substrates. Site-specific cleavage in vitro of the rpsT mRNA...
Spickler, Catherine, Mackie, George A.
The 3′→5′ exoribonucleases, RNase II and polynucleotide phosphorylase (PNPase), play an essential role in degrading fragments of mRNA generated by prior cleavages by endonucleases. We have...
Coburn, Glen A., Miao, Xin, Briant, Douglas J., Mackie, George A.
The RNA degradosome is a multiprotein complex required for the degradation of highly structured RNAs. We have developed a method for reconstituting a minimal degradosome from purified proteins. Our...
Function of the Conserved S1 and KH Domains in Polynucleotide Phosphorylase
Stickney, Leigh M., Hankins, Janet S., Miao, Xin, Mackie, George A.
We have examined the roles of the conserved S1 and KH RNA binding motifs in the widely dispersed prokaryotic exoribonuclease polynucleotide phosphorylase (PNPase). These domains can be released from...
Spickler, Catherine, Stronge, Victoria, Mackie, George A.
RNase E, the principal RNase capable of initiating mRNA decay, preferentially attacks 5′-monophosphorylated over 5′-triphosphorylated substrates. Site-specific cleavage in vitro of the rpsT mRNA...
Spickler, Catherine, Mackie, George A.
The 3′→5′ exoribonucleases, RNase II and polynucleotide phosphorylase (PNPase), play an essential role in degrading fragments of mRNA generated by prior cleavages by endonucleases. We have...
Coburn, Glen A., Miao, Xin, Briant, Douglas J., Mackie, George A.
The RNA degradosome is a multiprotein complex required for the degradation of highly structured RNAs. We have developed a method for reconstituting a minimal degradosome from purified proteins. Our...
Function of the Conserved S1 and KH Domains in Polynucleotide Phosphorylase
Stickney, Leigh M., Hankins, Janet S., Miao, Xin, Mackie, George A.
We have examined the roles of the conserved S1 and KH RNA binding motifs in the widely dispersed prokaryotic exoribonuclease polynucleotide phosphorylase (PNPase). These domains can be released from...
Role of RNA Structure and Susceptibility to RNase E in Regulation of a Cold Shock mRNA, cspA mRNA▿
Hankins, Janet S., Zappavigna, Christopher, Prud'homme-Généreux, Annie, Mackie, George A.
Degradation of the cspA mRNA in vivo is very rapid at temperatures greater than 30°C and is moderately dependent on RNase E. Investigations in vitro show that degradosomes prepared from normal or...
Chang, Samantha A., Cozad, Madeline, Mackie, George A., Jones, George H.
We examined the activity of polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor, Streptomyces antibioticus, and Escherichia coli in phosphorolysis using substrates derived from the...