Gregory Bokinsky

Single-molecule transition-state analysis of RNA folding

Bokinsky, Gregory, Rueda, David, Misra, Vinod K., Rhodes, Maria M., Gordus, Andrew, Babcock, Hazen P., ...

How RNA molecules fold into functional structures is a problem of great significance given the expanding list of essential cellular RNA enzymes and the increasing number of applications of RNA in...

Single-molecule enzymology of RNA: Essential functional groups impact catalysis from a distance

Rueda, David, Bokinsky, Gregory, Rhodes, Maria M., Rust, Michael J., Zhuang, Xiaowei, Walter, Nils G.

The hairpin ribozyme is a minimalist paradigm for studying RNA folding and function. In this enzyme, two domains dock by induced fit to form a catalytic core that mediates a specific backbone...

Single-molecule transition-state analysis of RNA folding

Bokinsky, Gregory, Rueda, David, Misra, Vinod K., Rhodes, Maria M., Gordus, Andrew, Babcock, Hazen P., ...

How RNA molecules fold into functional structures is a problem of great significance given the expanding list of essential cellular RNA enzymes and the increasing number of applications of RNA in...

Single-molecule enzymology of RNA: Essential functional groups impact catalysis from a distance

Rueda, David, Bokinsky, Gregory, Rhodes, Maria M., Rust, Michael J., Zhuang, Xiaowei, Walter, Nils G.

The hairpin ribozyme is a minimalist paradigm for studying RNA folding and function. In this enzyme, two domains dock by induced fit to form a catalytic core that mediates a specific backbone...

Dissecting the multistep reaction pathway of an RNA enzyme by single-molecule kinetic “fingerprinting”

Liu, Shixin, Bokinsky, Gregory, Walter, Nils G., Zhuang, Xiaowei

Single-molecule FRET is a powerful tool for probing the kinetic mechanism of a complex enzymatic reaction. However, not every reaction intermediate can be identified via a distinct FRET value, making...