Rao, Xiancai, Deighan, Padraig, Hua, Ziyu, Hu, Xiaomei, Wang, Jin, Luo, Miao, ...
The obligate intracellular human pathogen Chlamydia trachomatis undergoes a complex developmental program involving transition between two forms: the infectious elementary body (EB), and the rapidly...
Li, Zhongyu, Zhong, Youmin, Lei, Lei, Wu, Yimou, Wang, Shiping, Zhong, Guangming
Abstract Background C. trachomatis organisms carry a cryptic plasmid that encodes 8 open reading frames designated as pORF1 to 8. It is not clear whether all 8 pORFs are expressed during C....
Luo, Jianhua, Liu, Guangchao, Zhong, Youmin, Jia, Tianjun, Liu, Kaiyang, Chen, Ding, ...
Abstract Background Although more than 100 Chlamydia pneumoniae hypothetical proteins have been predicted to be inclusion membrane proteins, only a few have been experimentally demonstrated to be in...
Copyright American Society, Feng Dong, Heng Su, Yanqing Huang, Youmin Zhong, Guangming Zhong
lications that include ectopic pregnancy and infertility (22). Although how chlamydial infections cause diseases is still not fully understood, the hypothesis is that the long-term survival of...
Zhong, Guangming, E Sousa, Caetano Reis, Germain, Ronald N.
Several unanswered questions in T cell immunobiology relating to intracellular processing or in vivo antigen presentation could be approached if convenient, specific, and sensitive reagents were...
Zhang, Dongji, Yang, Xi, Lu, Hang, Zhong, Guangming, Brunham, Robert C.
As is true for other intracellular pathogens, immunization with live Chlamydia trachomatis generally induces stronger protective immunity than does immunization with inactivated organism. To...
Immunization with dendritic cells pulsed ex vivo with antigens has been successfully used to elicit primary antigen-specific immune responses. We report that mouse bone marrow-derived dendritic cells...
Wolf, Katerina, Fischer, Elizabeth, Mead, David, Zhong, Guangming, Peeling, Roseanna, Whitmire, Bill, ...
The major outer membrane protein (MOMP) of Chlamydia trachomatis serovariants is known to be an immunodominant surface antigen. Moreover, it is known that the C. trachomatis MOMP elicits antibodies...
Fan, Peiyi, Dong, Feng, Huang, Yanqing, Zhong, Guangming
Chlamydia pneumoniae is a causative agent for many respiratory infections and has been associated with cardiovascular diseases in humans. The pathogenicity of C. pneumoniae is thought to depend on...
Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis
Belland, Robert J., Zhong, Guangming, Crane, Deborah D., Hogan, Daniel, Sturdevant, Daniel, Sharma, Jyotika, ...
Chlamydia trachomatis is one of the most common bacterial pathogens and is the etiological agent of debilitating sexually transmitted and ocular diseases in humans. The organism is an obligate...
Chlamydia-Infected Cells Continue To Undergo Mitosis and Resist Induction of Apoptosis
Greene, Whitney, Xiao, Yangming, Huang, Yanqing, McClarty, Grant, Zhong, Guangming
Both anti- and proapoptotic activities have been reported to occur during chlamydial infection. To reconcile the apparent controversy, we compared host cell apoptotic responses to infection with 17...
Hu, He, Pierce, Grant N., Zhong, Guangming
Epidemiological investigations have linked Chlamydia pneumoniae infection to atherosclerosis. It is not clear, however, whether C. pneumoniae infection plays a causal role in the development of...
Cleavage of Host Keratin 8 by a Chlamydia-Secreted Protease
Dong, Feng, Su, Heng, Huang, Yanqing, Zhong, Youmin, Zhong, Guangming
Chlamydiae have to replicate within a cytoplasmic vacuole in eukaryotic cells. Expansion of the chlamydia-laden vacuole is essential for chlamydial intravacuolar replication, which inevitably causes...
Dong, Feng, Sharma, Jyotika, Xiao, Yanming, Zhong, Youmin, Zhong, Guangming
We previously identified a chlamydial protein designated CPAF (chlamydia protease/proteasome-like activity factor) that is secreted into host cell cytosol for degrading host transcription factors...
Chlamydia trachomatis Infection Inhibits Both Bax and Bak Activation Induced by Staurosporine
Xiao, Yangming, Zhong, Youmin, Greene, Whitney, Dong, Feng, Zhong, Guangming
We have previously shown that Chlamydia trachomatis inhibits host cell apoptosis and blocks mitochondrial cytochrome c release. We now report that activation of both Bax and Bak, two proapoptotic...
Human Antibody Responses to a Chlamydia-Secreted Protease Factor
Sharma, Jyotika, Bosnic, Anthony M., Piper, Jeanna M., Zhong, Guangming
We have previously identified a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor, or CPAF) in the cytosol of chlamydia-infected cells. Although CPAF is known...
Dong, Feng, Zhong, Youmin, Arulanandam, Bernard, Zhong, Guangming
We have previously identified a chlamydial protein, chlamydial protease/proteasome-like activity factor (CPAF), for degrading host transcription factors in cells infected with the human chlamydial...
Dong, Feng, Pirbhai, Mustak, Xiao, Yangming, Zhong, Youmin, Wu, Yimou, Zhong, Guangming
We have previously correlated Chlamydia trachomatis antiapoptotic activity with the blockade of mitochondrial cytochrome c release and the inhibition of Bax and Bak activation. We now report that C....
Sharma, Jyotika, Dong, Feng, Pirbhai, Mustak, Zhong, Guangming
We have previously shown that individuals infected with Chlamydia trachomatis can develop a robust antibody response to a chlamydia-secreted protein (designated chlamydial proteasome/protease-like...
Sharma, Jyotika, Zhong, Youmin, Dong, Feng, Piper, Jeanna M., Wang, Guqi, Zhong, Guangming
The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial...
Chen, Chaoqun, Chen, Ding, Sharma, Jyotika, Cheng, Wen, Zhong, Youmin, Liu, Kaiyang, ...
Using antibodies raised with chlamydial fusion proteins, we have localized a protein encoded by hypothetical open reading frame CT813 in the inclusion membrane of Chlamydia trachomatis. The detection...
Zhong, Guangming, E Sousa, Caetano Reis, Germain, Ronald N.
Several unanswered questions in T cell immunobiology relating to intracellular processing or in vivo antigen presentation could be approached if convenient, specific, and sensitive reagents were...
Zhang, Dongji, Yang, Xi, Lu, Hang, Zhong, Guangming, Brunham, Robert C.
As is true for other intracellular pathogens, immunization with live Chlamydia trachomatis generally induces stronger protective immunity than does immunization with inactivated organism. To...
Immunization with dendritic cells pulsed ex vivo with antigens has been successfully used to elicit primary antigen-specific immune responses. We report that mouse bone marrow-derived dendritic cells...
Wolf, Katerina, Fischer, Elizabeth, Mead, David, Zhong, Guangming, Peeling, Roseanna, Whitmire, Bill, ...
The major outer membrane protein (MOMP) of Chlamydia trachomatis serovariants is known to be an immunodominant surface antigen. Moreover, it is known that the C. trachomatis MOMP elicits antibodies...
Fan, Peiyi, Dong, Feng, Huang, Yanqing, Zhong, Guangming
Chlamydia pneumoniae is a causative agent for many respiratory infections and has been associated with cardiovascular diseases in humans. The pathogenicity of C. pneumoniae is thought to depend on...
Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis
Belland, Robert J., Zhong, Guangming, Crane, Deborah D., Hogan, Daniel, Sturdevant, Daniel, Sharma, Jyotika, ...
Chlamydia trachomatis is one of the most common bacterial pathogens and is the etiological agent of debilitating sexually transmitted and ocular diseases in humans. The organism is an obligate...
Chlamydia-Infected Cells Continue To Undergo Mitosis and Resist Induction of Apoptosis
Greene, Whitney, Xiao, Yangming, Huang, Yanqing, McClarty, Grant, Zhong, Guangming
Both anti- and proapoptotic activities have been reported to occur during chlamydial infection. To reconcile the apparent controversy, we compared host cell apoptotic responses to infection with 17...
Hu, He, Pierce, Grant N., Zhong, Guangming
Epidemiological investigations have linked Chlamydia pneumoniae infection to atherosclerosis. It is not clear, however, whether C. pneumoniae infection plays a causal role in the development of...
Cleavage of Host Keratin 8 by a Chlamydia-Secreted Protease
Dong, Feng, Su, Heng, Huang, Yanqing, Zhong, Youmin, Zhong, Guangming
Chlamydiae have to replicate within a cytoplasmic vacuole in eukaryotic cells. Expansion of the chlamydia-laden vacuole is essential for chlamydial intravacuolar replication, which inevitably causes...
Dong, Feng, Sharma, Jyotika, Xiao, Yanming, Zhong, Youmin, Zhong, Guangming
We previously identified a chlamydial protein designated CPAF (chlamydia protease/proteasome-like activity factor) that is secreted into host cell cytosol for degrading host transcription factors...
Chlamydia trachomatis Infection Inhibits Both Bax and Bak Activation Induced by Staurosporine
Xiao, Yangming, Zhong, Youmin, Greene, Whitney, Dong, Feng, Zhong, Guangming
We have previously shown that Chlamydia trachomatis inhibits host cell apoptosis and blocks mitochondrial cytochrome c release. We now report that activation of both Bax and Bak, two proapoptotic...
Human Antibody Responses to a Chlamydia-Secreted Protease Factor
Sharma, Jyotika, Bosnic, Anthony M., Piper, Jeanna M., Zhong, Guangming
We have previously identified a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor, or CPAF) in the cytosol of chlamydia-infected cells. Although CPAF is known...
Dong, Feng, Zhong, Youmin, Arulanandam, Bernard, Zhong, Guangming
We have previously identified a chlamydial protein, chlamydial protease/proteasome-like activity factor (CPAF), for degrading host transcription factors in cells infected with the human chlamydial...
Dong, Feng, Pirbhai, Mustak, Xiao, Yangming, Zhong, Youmin, Wu, Yimou, Zhong, Guangming
We have previously correlated Chlamydia trachomatis antiapoptotic activity with the blockade of mitochondrial cytochrome c release and the inhibition of Bax and Bak activation. We now report that C....
Sharma, Jyotika, Dong, Feng, Pirbhai, Mustak, Zhong, Guangming
We have previously shown that individuals infected with Chlamydia trachomatis can develop a robust antibody response to a chlamydia-secreted protein (designated chlamydial proteasome/protease-like...
Sharma, Jyotika, Zhong, Youmin, Dong, Feng, Piper, Jeanna M., Wang, Guqi, Zhong, Guangming
The available chlamydial genome sequences have made it possible to comprehensively analyze host responses to all chlamydial proteins, which is essential for further understanding of chlamydial...
Chen, Chaoqun, Chen, Ding, Sharma, Jyotika, Cheng, Wen, Zhong, Youmin, Liu, Kaiyang, ...
Using antibodies raised with chlamydial fusion proteins, we have localized a protein encoded by hypothetical open reading frame CT813 in the inclusion membrane of Chlamydia trachomatis. The detection...
Dong, Feng, Flores, Rhonda, Chen, Ding, Luo, Jianhua, Zhong, Youmin, Wu, Zhongming, ...
Using antibodies raised with chlamydial fusion proteins, we have localized a protein encoded by the hypothetical open reading frame Cpn0797 in the cytoplasm of Chlamydia pneumoniae-infected host...
Murthy, Ashlesh K., Cong, Yu, Murphey, Cathi, Guentzel, M. Neal, Forsthuber, Thomas G., Zhong, Guangming, ...
There is no licensed vaccine available against Chlamydia trachomatis, the leading cause of bacterial sexually transmitted disease. We have found that intranasal immunization with recombinant...
Hypothetical Protein Cpn0308 Is Localized in the Chlamydia pneumoniae Inclusion Membrane▿
Luo, Jianhua, Jia, Tianjun, Flores, Rhonda, Chen, Ding, Zhong, Guangming
The hypothetical protein encoded by Chlamydia pneumoniae open reading frame cpn0308 was detected in inclusion membranes of C. pneumoniae-infected cells using antibodies raised with Cpn0308 fusion...
Murthy, Ashlesh K., Chambers, James P., Meier, Patricia A., Zhong, Guangming, Arulanandam, Bernard P.
There is currently no licensed vaccine against Chlamydia trachomatis, the leading cause of sexually transmitted bacterial disease worldwide. Conventional vaccination attempts using surface-exposed...
Li, Weidang, Guentzel, M. Neal, Seshu, J., Zhong, Guangming, Murthy, Ashlesh K., Arulanandam, Bernard P.
An important consideration for antichlamydial vaccine development is the induction of cross-serovar protection, since multiple serovars (D to L) of Chlamydia trachomatis cause genital infections. We...
Zhong, Guangming, Fan, Tao, Liu, Li
We report that chlamydiae, which are obligate intracellular bacterial pathogens, can inhibit interferon (IFN)-γ–inducible major histocompatibility complex (MHC) class II expression. However, the...
Zhong, Guangming, Fan, Peiyi, Ji, Hezhao, Dong, Feng, Huang, Yanqing
Microbial pathogens have been selected for the capacity to evade or manipulate host responses in order to survive after infection. Chlamydia, an obligate intracellular pathogen and the causative...
Zhong, Guangming, Romagnoli, Paola, Germain, Ronald N.
Leucine-based signals in the cytoplasmic tail of invariant chain (Ii) control targeting of newly synthesized major histocompatibility complex class II molecules to the endocytic pathway for...
Zhong, Guangming, Sousa, Caetano Reis E, Germain, Ronald N.
Intravenous (i.v.) injection of high amounts of soluble proteins often results in the induction of antigen-specific tolerance or deviation to helper rather than inflammatory T cell immunity. It has...
Fan, Tao, Lu, Hang, Hu, He, Shi, Lianfa, McClarty, Grant A., Nance, Dwight M., ...
We report that chlamydiae, which are obligate intracellular bacterial pathogens, possess a novel antiapoptotic mechanism. Chlamydia-infected host cells are profoundly resistant to apoptosis induced...
Zhong, Guangming, Liu, Li, Fan, Tao, Fan, Peiyi, Ji, Hezhao
We have previously shown that the obligate intracellular pathogen chlamydia can suppress interferon (IFN)-γ–inducible major histocompatibility complex (MHC) class II expression in infected cells...
Cheng, Wen, Shivshankar, Pooja, Li, Zhongyu, Chen, Lili, Yeh, I-Tien, Zhong, Guangming
Chlamydia trachomatis infection induces inflammatory pathologies in the upper genital tract, potentially leading to ectopic pregnancy and infertility in the affected women. Caspase-1 is required for...
Shivshankar, Pooja, Lei, Lei, Wang, Jie, Zhong, Guangming
We report that rottlerin, a plant-derived compound known to inhibit various mammalian kinases, profoundly inhibited chlamydial growth in cell culture with a minimal inhibition concentration of 1 μM....
Cheng, Wen, Shivshankar, Pooja, Zhong, Youmin, Chen, Ding, Li, Zhongyu, Zhong, Guangming
Chlamydia trachomatis infection induces a wide array of inflammatory cytokines and chemokines, which may contribute to chlamydia-induced pathologies. However, the precise mechanisms by which...
Li, Zhongyu, Chen, Chaoqun, Chen, Ding, Wu, Yimou, Zhong, Youmin, Zhong, Guangming
Although the Chlamydia trachomatis genome is predicted to encode 50 inclusion membrane proteins, only 18 have been experimentally localized in the inclusion membrane of C. trachomatis-infected cells....
Li, Zhongyu, Chen, Ding, Zhong, Youmin, Wang, Shiping, Zhong, Guangming
The chlamydial cryptic plasmid encodes eight putative open reading frames (ORFs), designated pORF1 to -8. Antibodies raised against these ORF proteins were used to localize the endogenous proteins...
Rao, Xiancai, Deighan, Padraig, Hua, Ziyu, Hu, Xiaomei, Wang, Jin, Luo, Miao, ...
The obligate intracellular human pathogen Chlamydia trachomatis undergoes a complex developmental program involving transition between two forms: the infectious elementary body (EB), and the rapidly...