H. Lehrach

Details der Publikationsliste

Zeitraum

1980 - 2008

Anzahl

117

Co-Autoren

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo (2008)

Kues, W. A., Sudheer, S., Herrmann, D., Carnwath, J. W., Havlicek, V., Besenfelder, U., ...

Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of...

Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress (2007)

Ralser, Markus, Wamelink, Mirjam M., Kowald, Axel, Gerisch, Birgit, Heeren, Gino, Struys, Eduard A., ...

Background Eukaryotic cells have evolved various response mechanisms to counteract the deleterious consequences of oxidative stress. Among these processes, metabolic alterations seem to play an...

High-throughput identification of potential Arabidopsis MAP kinases substrates (2005)

Feilner, T, Hultschig, C., Lee, J., Meyer, S., Immink, R.G.H., Koenig, A., ...

Mitogen-activated protein kinase (MAPK) cascades are universal and highly conserved signal transduction modules in eucaryotes, including plants. These protein phosphorylation cascades link...

Versatile gene-specific sequence tags for Arabidopsis functional genomics: Trancript profiling and reverse genetics applications (2004)

Hilson, P., Allemeersch, J., Altmann, T, Aubourg, S, Avon, A., Beynon, J., ...

Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization...

Establishment of T cell-specific and natural killer cell-specific unigene sets: towards high-throughput genomics of leukaemia (2004)

Illiger, J., Herwig, R., Steinfath, M., Przewieslik, T., Elge, T., Bull, C., ...

We report the establishment of highly non-redundant unigene sets consisting of cDNA clones derived from T lymphocytes and natural killer cells. Each set consists of 10 506 and 13 409 clones,...

Mutations in DNAH5 cause primary ciliary dyskinesia and randomization of left–right asymmetry (2002)

Olbrich, H., Häffner, K., Kispert, A., Völkel, A., Volz, A., Sasmaz, G., ...

Primary ciliary dyskinesia (PCD, MIM 242650) is characterized by recurrent infections of the respiratory tract due to reduced mucociliary clearance and by sperm immobility. Half of the affected...

The genome sequence of Schizosaccharomyces pombe (2002)

Gwilliam, R, Rajandream, M-A, Lyne, M, Lyne, R, Stewart, A, ...

We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The...

The genome sequence of Schizosaccharomyces pombe (2002)

Wood, V., Gwilliam, R., Rajandream, M. A., Lyne, M., Lyne, R., Stewart, A., ...

We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The...

The genome sequence of Schizosaccharomyces pombe (2002)

Wood, V., Gwilliam, R., Rajandream, M. A., Lyne, M., Lyne, R., Stewart, A., ...

We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The...

Initial sequencing and analysis of the human genome (2001)

Lander, ES, Linton, LM, Birren, B, Nusbaum, C, Zody, MC, Baldwin, J, ...

The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and...

The isolation of cDNAs within the Huntington disease region by hybridisation of yeast artificial chromosomes to a cDNA library (1993)

Snell, R.G., Doucette-Stamm, L.A., Gillespie, K.M., Taylor, S.A.M., Riba, L., Bates, G.P., ...

Flanking recombination events have defined the Huntington Disease gene candidate region to between D4S10 and D4S98, about 2.2Mb. Because of the large size of the candidate region and the likely large...

Radiation hybrid map spanning the huntington disease gene region of chromosome 4 (1992)

Altherr, Michael R., Altherr, Michael R., Plummer, Sarah, Plummer, Sarah, Bates, G., Bates, G., ...

Radiation hybrid (RH) mapping was used to construct a map of 11 markers in the distal 4 Mb of the short arm of chromosome 4, the region containing the Huntington disease gene. Two different methods...

A YAC contig across the fragile X site defines the region of fragility (1991)

Hirst, M.C., Rack, K., Nakahori, Y., Roche, A., Bell, M.V., Flynn, G., ...

The fragile X syndrome is a common cause of mental retardation and Is associated with a fragile site at Xq27.3 (FRAXA). Recently, evidence has been presented for the role of methylation and genomlc...

The HOX-5 and surfeit gene clusters are linked in the proximal portion of mouse chromosome 2 (1990)

Stubbs, L., Huxley, C., Hogan, B., Evans, T., Fried, M., Duboule, D., ...

Using an interspecies backcross, we have mapped the HOX-5 and surfeit (surf) gene clusters within the proximal portion of mouse chromosome 2. While the HOX-5 cluster of homeobox-containing genes has...

The murine genes Hox-5.1 and Hox-4.1 belong to the same HOX complex on chromosome 2 (1990)

Stubbs, L., Poustka, A., Baron, A., Lehrach, H., Lonai, P., Duboule, D.

Two different loci of Antennapedia-related homeobox-containing genes have been shown to map to mouse chromosome 2: the HOX-5 complex and the Hox-4.1 gene. These independently derived loci are likely...

Ordering of cosmid clones covering the Herpes Simplex virus type I (HSV-I) genome: a test case for fingerprinting by hybridisation (1990)

Carig, A.G., Nizetic, D., Hoheisel, J.D., Zehetner, G., Lehrach, H.

To allow the efficient construction of ordered clone libraries, we have been investigating the use of ‘oligonucleotide fingerprinting’ as an approach to identify overlapping clones, and...

Construction of a NotI linking library and isolation of new markers close to the Huntington's disease gene (1988)

Pohl, T.M., Zimmer, M., MacDonald, M.E., Smith, B., Bucan, M., Poustka, A., ...

Linking clones contain sequences flanking recognition sites for enzymes cutting rarely in mammalian DNA. They can be used to obtain and correlate both physical and genetic mapping information over...

Brain tubutin and actin cDNA sequence: isolation of recombinant plasmids (1980)

Ginzburg, I., De Baetselier, A., Walker, M.D., Behar, L., Lehrach, H., Frischauf, A.M., ...

Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pstl site of pBR322...

A subcloning strategy for DNA sequence analysis (1980)

Frischauf, A.M., Garoff, H., Lehrach, H.

We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst...

Physical mapping, cloning, and identification of genes within a 500-kb region containing BRCA1.

Brown, M A, Jones, K A, Nicolai, H, Bonjardim, M, Black, D, McFarlane, R, ...

BRCA1 is a breast/ovarian cancer susceptibility gene on human chromosome 17q21. We describe a complete and detailed physical map of a 500-kb region of genomic DNA containing the BRCA1 gene and the...

Construction, arraying, and high-density screening of large insert libraries of human chromosomes X and 21: their potential use as reference libraries.

Nizetić, D, Zehetner, G, Monaco, A P, Gellen, L, Young, B D, Lehrach, H

We have constructed cosmid libraries from flow-sorted human chromosomes X and 21, each of which contains greater than 30 genome equivalents, and have developed systems allowing permanent storage of...

Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.

Larin, Z, Monaco, A P, Lehrach, H

Yeast artificial chromosome (YAC) libraries have been difficult to construct with average insert sizes greater than 400 kilobase pairs when DNA is size-fractionated in low-melting-point agarose. By...

Physical map of human Xq27-qter: localizing the region of the fragile X mutation.

Poustka, A, Dietrich, A, Langenstein, G, Toniolo, D, Warren, S T, Lehrach, H

We describe a physical map of the end of the long arm of the human X chromosome encompassing the region from Xq27.2 to the q telomere, inclusive of the chromosomal band Xq28. This region is of...

A yeast artificial chromosome containing the mouse homeobox cluster Hox-2.

Rubock, M J, Larin, Z, Cook, M, Papalopulu, N, Krumlauf, R, Lehrach, H

We have isolated two genes, Hox-2.8 and Hox-2.9, from the mouse homeobox cluster Hox-2, located on chromosome 11. A 120-kilobase yeast artificial chromosome (YAC) containing a large region of the...

Simplified handling of high-density genetic filters using rigid plastic laminates.

Bancroft, D R, O'Brien, J K, Guerasimova, A, Lehrach, H

Wet membranes in DNA hybridisation procedures can be very difficult to handle. This adds considerable time to the length of experiments and is a serious obstacle to the performance of large-scale...

IXDB, an X chromosome integrated database.

Leser, U, Wagner, R, Grigoriev, A, Lehrach, H, Roest Crollius, H

The integrated X chromosome database (IXDB) is a repository for physical mapping data of the human X chromosome. Its current content is the result of a strict integration of data stemming from many...

Comparative gene expression profiling by oligonucleotide fingerprinting.

Meier-Ewert, S, Lange, J, Gerst, H, Herwig, R, Schmitt, A, Freund, J, ...

The use of hybridisation of synthetic oligonucleotides to cDNAs under high stringency to characterise gene sequences has been demonstrated by a number of groups. We have used two cDNA libraries of 9...

A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library.

Büssow, K, Cahill, D, Nietfeld, W, Bancroft, D, Scherzinger, E, Lehrach, H, ...

We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally...

Preselection of shotgun clones by oligonucleotide fingerprinting: an efficient and high throughput strategy to reduce redundancy in large-scale sequencing projects.

Radelof, U, Hennig, S, Seranski, P, Steinfath, M, Ramser, J, Reinhardt, R, ...

Large-scale genomic sequencing projects generally rely on random sequencing of shotgun clones, followed by different gap closing strategies. To reduce the overall effort and cost of those projects...

IXDB, an X chromosome integrated database (update).

Leser, U, Roest Crollius, H, Lehrach, H, Sudbrak, R

Chromosome specific databases are an important research tool as they integrate data from different directions, such as genetic and physical mapping data, expression data, sequences etc. They...

A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation.

Guerasimova, A, Ivanov, I, Lehrach, H

Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with...

Nucleotide sequence at the junction between the nonstructural and the structural genes of the semliki forest virus genome.

Riedel, H, Lehrach, H, Garoff, H

The nucleotide sequence at the junction between the nonstructural and the structural genes of the Semliki Forest virus 42S RNA genome has been determined from cloned cDNA. With the aid of S1-mapping,...

Fine-mapping of shotgun template-libraries; an efficient strategy for the systematic sequencing of genomic DNA.

Scholler, P, Karger, A E, Meier-Ewert, S, Lehrach, H, Delius, H, Hoheisel, J D

To test the effectiveness of ordering shotgun DNA-templates prior to sequence analysis, the 450 kb left arm of yeast chromosome XII was randomly subcloned into a phagemid vector. Clones were ordered...

Algorithms and software tools for ordering clone libraries: application to the mapping of the genome of Schizosaccharomyces pombe.

Mott, R, Grigoriev, A, Maier, E, Hoheisel, J, Lehrach, H

A complete set of software tools to aid the physical mapping of a genome has been developed and successfully applied to the genomic mapping of the fission yeast Schizosaccharomyces pombe. Two...

Labelling oligonucleotides to high specific activity (I).

Craig, A G, Nizetic, D, Lehrach, H

The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per...

Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.

Ginzburg, I, De Baetselier, A, Walker, M D, Behar, L, Lehrach, H, Frischauf, A M, ...

Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322...

A subcloning strategy for DNA sequence analysis.

Frischauf, A M, Garoff, H, Lehrach, H

We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst...

A procedure to verify an amino acid sequence which has been derived from a nucleotide sequence: application to the 26S RNA of Semliki Forest virus.

Garoff, H, Riedel, H, Lehrach, H

We describe a peptide sequencing procedure which can be used to verify an amino acid sequence which is derived from a nucleotide sequence. One first labels the protein with a 3H- and a 14C-labelled...

Construction and characterization of type II collagen complementary deoxyribonucleic acid clones.

Lukens, L N, Frischauf, A M, Pawlowski, P J, Brierley, G T, Lehrach, H

The mRNA for type II collagen was purified from embryonic chick sternum or from purified sternal chondrocytes with guanidine thiocyanate as the extractant. Double-stranded cDNAs to procollagen mRNAs...

Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

Monaco, A P, Lam, V M, Zehetner, G, Lennon, G G, Douglas, C, Nizetic, D, ...

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method...

A novel and rapid method for isolating sequences adjacent to rare cutting sites and their use in physical mapping.

Patel, K, Cox, R, Shipley, J, Kiely, F, Frazer, K, Cox, D R, ...

We describe a simple PCR based technique which can be used to isolate sequences adjacent to rare cutter sites and can subsequently be employed for the construction of long range physical maps. The...

W (A or T) sequences as probes and primers suitable for genomic mapping and fingerprinting.

Drmanac, R, Nizetic, D, Lennon, G G, Beitverda, A, Lehrach, H

A limitation to the use of oligonucleotide probes as tools for genetic and physical mapping has been the low hybridization positive frequency obtained by oligonucleotides of sufficient length to...

Ordering of cosmid clones covering the herpes simplex virus type I (HSV-I) genome: a test case for fingerprinting by hybridisation.

Craig, A G, Nizetic, D, Hoheisel, J D, Zehetner, G, Lehrach, H

To allow the efficient construction of ordered clone libraries, we have been investigating the use of 'oligonucleotide fingerprinting' as an approach to identify overlapping clones, and ultimately...

A method for the generation of YAC transgenic mice by pronuclear microinjection.

Schedl, A, Larin, Z, Montoliu, L, Thies, E, Kelsey, G, Lehrach, H, ...

Yeast artificial chromosomes (YACs) represent the latest generation of vectors which have the great advantage of large insert size. The introduction of YACs into mammalian cells and organisms has...

Control of partial digestion combining the enzymes dam methylase and MboI.

Hoheisel, J D, Nizetic, D, Lehrach, H

A method is described which allows the preparation of reproducible partial digests without previous establishment of the incubation conditions. It is based on a combined application of dam methylase...

A long range restriction map of the pseudoautosomal region by partial digest PFGE analysis from the telomere.

Rappold, G A, Lehrach, H

The analysis of partial digestion products extending from the telomere of the human X and Y chromosomes, visualised by hybridisation to a probe located close to the telomere, was used to establish a...

A computer program package for restriction map analysis and manipulation.

Zehetner, G, Lehrach, H

Programs for the calculation, storage and analysis of restriction maps derived from the analysis of partial digestion products from end labelled DNA (1,2,3) and their correlation with digestion - and...

Selective isolation of cosmid clones by homologous recombination in Escherichia coli.

Poustka, A, Rackwitz, H R, Frischauf, A M, Hohn, B, Lehrach, H

A procedure for selection of specific cosmid clones by homologous recombination between cosmid clones from a library and sequences cloned into a plasmid has been developed. Cosmid libraries...

Molecular cloning and expression during myogenesis of sequences coding for M-creatine kinase.

Rosenberg, U B, Kunz, G, Frischauf, A, Lehrach, H, Mähr, R, Eppenberger, H M, ...

Sequences complementary to muscle poly(A)+RNA were cloned in the plasmid pBR322 and the resulting colonies were screened by colony hybridization with labeled cDNA derived from skeletal muscle and...

Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.

Katcoff, D, Nudel, U, Zevin-Sonkin, D, Carmon, Y, Shani, M, Lehrach, H, ...

The construction and partial characterization of recombinant bacterial plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin light chain mRNA is described. DNA of...

The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region.

Garoff, H, Frischauf, A M, Simons, K, Lehrach, H, Delius, H

The amino acid sequence of the capsid (C) protein was deduced from the nucleotide sequence of the C gene. This part of the viral 42S RNA genome was transcribed into double-stranded cDNA. The cDNA was...

Construction and characterization of a 2.5-kilobase procollagen clone.

Lehrach, H, Frischauf, A M, Hanahan, D, Wozney, J, Fuller, F, Crkvenjakov, R, ...

Recombinant bacterial plasmids have been constructed by inserting double-stranded chicken procollagen cDNA sequences linked to chemically synthesized decanucleotides containing HindIII sites into the...

Genetic and molecular mapping of the Hmt region of mouse.

Richards, S, Bucan, M, Brorson, K, Kiefer, M C, Hunt, S W, Lehrach, H, ...

We have mapped a new region of the mouse major histocompatibility complex (MHC) that contains the nuclear gene, Hmt, for the maternally transmitted antigen, Mta. The Hmt region of chromosome 17 lies...

Deletions in immunoglobulin mu chains.

Köhler, G, Potash, M J, Lehrach, H, Shulman, M J

Eight mutant hybridoma lines are described, which synthesize short immunoglobulin mu chains. Four internal deletions were mapped by Southern blot analysis. They are shown to remove DNA from either...

Constant and variable parts in the Balbiani ring 2 repeat unit and the translation termination region

Jäckle, H., Almeida, J. C. De, Galler, R., Kluding, H., Lehrach, H.

The large Balbiani rings of the chironomids produce giant internally repeated transcripts that are translated into silk-like proteins used for protective tubes. We have cloned fragments of the...

Close genetic and physical linkage between the murine haemopoietic growth factor genes GM-CSF and Multi-CSF (IL3).

Barlow, D P, Bućan, M, Lehrach, H, Hogan, B L, Gough, N M

The two murine haemopoietic growth factors, granulocyte-macrophage colony stimulating factor (GM-CSF) and Multi-CSF (interleukin 3) stimulate the proliferation and differentiation of an overlapping...

Identification of a testis-specific gene from the mouse t-complex next to a CpG-rich island.

Rappold, G A, Stubbs, L, Labeit, S, Crkvenjakov, R B, Lehrach, H

We have used an approach based on the observation of CpG-rich regions near the 5' end of many genes to screen a panel of cosmids derived from the t-complex and tested candidate sequences for evidence...

Genetic and cytogenetic localisation of the homeo box containing genes on mouse chromosome 6 and human chromosome 7.

Bućan, M, Yang-Feng, T, Colberg-Poley, A M, Wolgemuth, D J, Guenet, J L, Francke, U, ...

Probes from the m6 homeo box cluster were mapped to mouse chromosome 6 by somatic cell genetics, in situ hybridisation, and by a Mus spretus--Mus musculus backcross mapping system. In addition, the...

Fine mapping of the McLeod locus (XK) to a 150-380-kb region in Xp21.

Ho, M F, Monaco, A P, Blonden, L A, Van Ommen, G J, Affara, N A, Ferguson-Smith, M A, ...

McLeod syndrome, characterized by acanthocytosis and the absence of a red-blood-cell Kell antigen (Kx), is a multisystem disorder involving a late-onset myopathy, splenomegaly, and neurological...

Complex patterns of linkage disequilibrium in the Huntington disease region.

MacDonald, M E, Lin, C, Srinidhi, L, Bates, G, Altherr, M, Whaley, W L, ...

The genetic defect causing Huntington disease (HD) has been mapped to 4p16.3 by linkage analysis using DNA markers. Two apparently contradictory classes of recombination events in HD kindreds...

Defined physical limits of the Huntington disease gene candidate region.

Bates, G P, MacDonald, M E, Baxendale, S, Youngman, S, Lin, C, Whaley, W L, ...

The Huntington disease (HD) gene has been mapped 4 cM distal to D4S10 within the telomeric chromosome band, 4p16.3. The published physical map of this region extends from D4S10 to the telomere but...

A yeast artificial chromosome telomere clone spanning a possible location of the Huntington disease gene

Bates, G. P., MacDonald, M. E., Baxendale, S., Sedlacek, Z., Youngman, S., Romano, D., ...

The Huntington disease (HD) gene has been mapped to the most distal subband of chromosome 4p. Analysis of recombination events has not provided an unequivocal location of the HD gene, but it...

Aggregation of truncated GST-HD exon 1 fusion proteins containing normal range and expanded glutamine repeats.

Hollenbach, B, Scherzinger, E, Schweiger, K, Lurz, R, Lehrach, H, Wanker, E E

We have shown previously by electron microscopy that the purified glutathione S-transferase (GST)-Huntington's disease (HD) exon 1 fusion protein with 51 glutamine residues (GST-HD51) is an oligomer,...

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Kues, W. A., Sudheer, S., Herrmann, D., Carnwath, J. W., Havlicek, V., Besenfelder, U., ...

Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of...

Stemming Cancer: Functional Genomics of Cancer Stem Cells in Solid Tumors

Regenbrecht, C. R. A., Lehrach, H., Adjaye, J.

Cancer stem cells (CSCs) were discovered about 15 years ago in hematopoietic cancers. Subsequently, cancer stem cells were discovered in various solid tumors. Based on parallels with normal stem...