Site-specific labeling of the ribosome for single-molecule spectroscopy (2005)
Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach...
Ligand-induced conformational changes observed in single RNA molecules
Ha, Taekjip, Zhuang, Xiaowei, Kim, Harold D., Orr, Jeffrey W., Williamson, James R., Chu, Steven
We present the first demonstration that fluorescence resonance energy transfer can be used to track the motion of a single molecule undergoing conformational changes. As a model system, the...
Fluorescence quenching: A tool for single-molecule protein-folding study
Zhuang, Xiaowei, Ha, Taekjip, Kim, Harold D., Centner, Thomas, Labeit, Siegfried, Chu, Steven
By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye...
Kim, Harold D., Nienhaus, G. Ulrich, Ha, Taekjip, Orr, Jeffrey W., Williamson, James R., Chu, Steven
Fluorescence correlation spectroscopy (FCS) of fluorescence resonant energy transfer (FRET) on immobilized individual fluorophores was used to study the Mg2+-facilitated conformational change of an...
tRNA dynamics on the ribosome during translation
Blanchard, Scott C., Kim, Harold D., Gonzalez, Ruben L., Puglisi, Joseph D., Chu, Steven
Using single-molecule fluorescence spectroscopy, time-resolved conformational changes between fluorescently labeled tRNA have been characterized within surface-immobilized ribosomes proceeding...
Site-specific labeling of the ribosome for single-molecule spectroscopy
Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach...
Ligand-induced conformational changes observed in single RNA molecules
Ha, Taekjip, Zhuang, Xiaowei, Kim, Harold D., Orr, Jeffrey W., Williamson, James R., Chu, Steven
We present the first demonstration that fluorescence resonance energy transfer can be used to track the motion of a single molecule undergoing conformational changes. As a model system, the...
Fluorescence quenching: A tool for single-molecule protein-folding study
Zhuang, Xiaowei, Ha, Taekjip, Kim, Harold D., Centner, Thomas, Labeit, Siegfried, Chu, Steven
By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye...
Kim, Harold D., Nienhaus, G. Ulrich, Ha, Taekjip, Orr, Jeffrey W., Williamson, James R., Chu, Steven
Fluorescence correlation spectroscopy (FCS) of fluorescence resonant energy transfer (FRET) on immobilized individual fluorophores was used to study the Mg2+-facilitated conformational change of an...
tRNA dynamics on the ribosome during translation
Blanchard, Scott C., Kim, Harold D., Gonzalez, Ruben L., Puglisi, Joseph D., Chu, Steven
Using single-molecule fluorescence spectroscopy, time-resolved conformational changes between fluorescently labeled tRNA have been characterized within surface-immobilized ribosomes proceeding...
Site-specific labeling of the ribosome for single-molecule spectroscopy
Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach...
The role of fluctuations in tRNA selection by the ribosome
Lee, Tae-Hee, Blanchard, Scott C., Kim, Harold D., Puglisi, Joseph D., Chu, Steven
The detailed mechanism of how the ribosome decodes protein sequence information with an abnormally high accuracy, after 40 years of study, remains elusive. A critical element in selecting correct...
Fluctuations of Transfer RNAs between Classical and Hybrid States
Kim, Harold D., Puglisi, Joseph D., Chu, Steven
Adjacent transfer RNAs (tRNAs) in the A- and P-sites of the ribosome are in dynamic equilibrium between two different conformations called classical and hybrid states before translocation. Here, we...