The atmospheric concentration of CO2 will probably rise to about 700 µmol mol–1 by the end of this century. The effects of elevated growth CO2 on photosynthesis are still not fully understood....
Kobayashi, Isao, Fujiwara, Shoko, Shimogawara, Kosuke, Sakuma, Chiseko, Shida, Yasuo, Kaise, Toshikazu, ...
Pi in the medium relieved toxicity of arsenate against cellular growth of Chlamydomonas reinhardtii. To investigate the relationship between intracellular P contents and arsenate resistance, we...
The atmospheric concentration of CO2 will likely rise to about 700 µmol mol-1 by the end of this century. Effects of elevated growth CO2 on photosynthesis is still not fully understood. Effects of...
Kobayashi, Isao, Fujiwara, Shoko, Shimogawara, Kosuke, Sakuma, Chiseko, Shida, Yasuo, Kaise, Toshikazu, ...
Pi in the medium relieved the toxicity of arsenate against cellular growth of Chlamydomonas reinhardtii. To investigate the relationship between intracellular P contents and arsenate resistance, we...
Kobayashi, Isao, Fujiwara, Shoko, Shimogawara, Kosuke, Sakuma, Chiseko, Shida, Yasuo, Kaise, Toshikazu, ...
Pi in the medium relieved toxicity of arsenate against cellular growth of Chlamydomonas reinhardtii. To investigate the relationship between intracellular P contents and arsenate resistance, we...
The atmospheric concentration of CO2 will likely rise to about 700 µmol mol-1 by the end of this century. Effects of elevated growth CO2 on photosynthesis is still not fully understood. Effects of...
Kobayashi, Isao, Fujiwara, Shoko, Shimogawara, Kosuke, Kaise, Toshikazu, Usuda, Hideaki, Tsuzuki, Mikio
An arsenate-resistant mutant AR3 of Chlamydomonas reinhardtii is a recessive mutant generated by random insertional mutagenesis using the ARG7 gene. AR3 shows about 10-fold resistance against...
Sawada, Shinichi, Sakamoto, Takeshi, Sato, Makiko, Kasai, Minobu, Usuda, Hideaki
We have studied source-sink relationships with a model consisting of single-rooted leaves without petioles. We previously reported that the rate of photosynthesis decreased when C4 model plants...
Rouhier, Hervé, Usuda, Hideaki
Sucrose synthase (SuSy) is a key enzyme in the development of storage root of radish. Clarification of its spatial and temporal expression during the thickening growth of radish hypocotyl, which...
Usuda, Hideaki, Demura, Taku, Shimogawara, Kousuke, Fukuda, Hiroo
The mechanisms that control sink capacity are poorly understood. in radish, a major sink is the “storage root”, which begins to thicken early in development, mainly as a result of thickening of...
Usuda, Hideaki, Rouhier, Hervé, Demura, Taku, Fukuda, Hiroo
Controling mechanisms of sink capacity are poorly understood. Previously we suggested that sucrose synthase (SuSy), but not invertase, plays an important role for sink capacity of the radish...
Sawada, Shinichi, Arakawa, Osamu, Muraki, Isao, Echigo, Hitoshi, Miyashita, Miyuki, Iwafune, Mito, ...
C4 model plants composed of single-rooted Amarantus cruentus L. leaves were developed to study source-sink relationships. The photosynthetic activity of CO4 fixation in the rooted leaves cultured...
Usuda, Hideaki, Shimogawara, Kousuke
The effects of sink capacity on the regulation of the acclimation of photosynthetic capacity to elevated levels of carbon dioxide are important from a global perspective. We investigated the effeocts...
The effects of phosphorus (P) deprivation on the changes in the contents of reduced nitrogen (N) and P in the shoots of young maize plants (Zea mays L.) were investigated for up to 30 days after...
Usuda, Hideaki, Shimogawara, Kousuke
The effects of P deprivation on the two-dimensional electrophoretic patterns of soluble proteins were evaluated in maize (Zea mays L.) leaves. P deprivation resulted in decreases in the relative...
Anwaruzzaman,, Sawada, Shinichi, Usuda, Hideaki, Yokota, Akiho
The mechanism of the regulation of the activation of ribulose 1,5-bisphosphate carboxylase/ oxygenase (RuBisCO) by inorganic phosphate (Pi) in the presence of limiting concentrations of CO2 was...
Shimogawara, Kousuke, Usuda, Hideaki
The kinetics of Pi uptake by phosphate-starved and non-starved tobacco cells (Nicotiana tabacum BY-2) suspension culture was investigated. The kinetic parameters of Pi uptake were determined by...
Yokota, Akiho, Higashioka, Masaya, Taira, Tomoaki, Usuda, Hideaki, Wadano, Akira, Murayama, Hiroshi
This is the first report showing that ribulose bisphosphate carboxylase/oxygenase has the non-catalytic sites to bind ribulose bisphosphate (RuBP). A plot of the binding number against the RuBP...
Usuda, Hideaki, Shimogawara, Kousuke
When frozen leaves of 24-day-old maize (Zea mays L.) plant were thawed on moist filter paper at 26°C (freeze-thaw treatment) several enzymes, including phosphoenolpyruvate carboxylase (PEPC) and...
Usuda, Hideaki, Shimogawara, Kousuke
With low-P treatment of maize, the level of sucrose phosphate synthase (SPS) protein decreased to 15% of the control, while the “Vmax” activity stayed relatively high (100–80% of the control)...
Sawada, Shinichi, Usuda, Hideaki, Tsukui, Takahiro
Sink-limited conditions, defined as treatment with continuous illumination, cause a reduction in the rate of photosynthetic fixation of CO2 in single-rooted leaves of soybean (Glycine max. Merr. cv....
Usuda, Hideaki, Shimogawara, Kousuke
Maize plants (Zea mays L.) were cultured with nutrient solution containing 0.001 or 0.5 mM orthophosphate (Pi). Effects of low phosphate (low-P) nutrition on growth, leaf phosphate status,...
Phosphate Deficiency in Maize. II. Enzyme Activities (1991)
Usuda, Hideaki, Shimogawara, Kousuke
Low-phosphate (P) treatment decreased photosynthetic rates of maize plants (Zea mays L.) by about 50% 18 to 19 days after planting [Usuda and Shimogawara (1991) Plant Cell Physiol. 32: 497]. Low-P...
Sawada, Shinichi, Usuda, Hideaki, Hasegawa, Yasuhide, Tsukui, Takahiro
The results of our previous study [Sawada et al. (1989) Plant Cell Physiol. 30: 691] implied that, under sink-limited conditions, a decrease in the activity of ribulose-l,5-bisphosphate carboxylase...
Imaizumi, Nobuyuki, Usuda, Hideaki, Nakamoto, Hitoshi, Ishihara, Kuni
Photosynthesis is known to occur in rice panicles, but little has been reported about the photosynthetic or biochemical characteristics of such panicles. The estimated gross amount of...
A possible mechanism for the previous finding of a transient peak of ribulose 1,5-bisphosphate (RuBP) in maize leaves during induction of photosynthesis [Usuda (1985) Plant Physiol. 78: 859] was...
A spectrophotometric procedure for assay of initial and total activity of ribulose 1,5-bisphosphate carboxylase in maize leaves was established. The extraction of the crude enzyme from maize leaf...
Highly good correlations for the extractable activities of ribulose-1,5-bisphosphate carboxylase, pyruvate,Pi dikinase, phosphoenolpyruvate carboxylase, NADP-malate dehydrogenase and NADP-malic...
Bryophyllum diagremontianum plants grown under light-dark regimes were exposed to one more cycle of the regime or to continuous darkness for 24 hr. Photosynthetic O2 evolution by leaf segments from...
USUDA, HIDEAKI, ARRON, GEOFFREY P., EDWARDS, GERALD E.
Aminoacetonitrile was found to be a competitive inhibitor of glycine oxidation by isolated wheat leaf mitochondria (K1 = 126 μM). The decarboxylation of glycine by wheat protoplasts was also...
Usuda, Hideaki, Miyachi, Shigetoh
In the presence of NADP+ and Mg++, the bundle sheath strands isolated from corn (Zea mays) leaves by cellulase treatments decarboxylated malate in the light at an initial rate (200 μmoles/mg...
Usuda, Hideaki, Kanai, Ryuzi, Miyachi, Shigetoh
The activities of Hill reaction and photosynthetic 14CO2 fixation in bundle sheath strands enzymatically isolated from millet (Panicum miliaceum) were 3–15 times as high as those observed in corn...
Usuda, Hideaki, Matsushima, Hisashi, Miyachi, Shigetoh
Mesophyll chloroplasts capable of assimilating 1.2 μmoles CO2 per milligram chlorophyll per hour were isolated from 7-day-old corn (Zea mays, Nagano No. 1) leaves. Addition of phosphoenolpyruvate...
Usuda, Hideaki, Samejima, Muneaki, Miyachi, Shigetoh
Most of the radioactivity incorporated into malic acid during light-enhanced dark 14CO2-fixation was found in C-4, supporting our conclusion that phosphoenolpyruvic acid serves as a primary acceptor...
USUDA, HIDEAKI, KANAI, RYUZI, TAKEUCHI, MASAYUKI
The pattern for primary products of CO2-fixation and the chloroplast structure of Amaranthus retrqflexus L., a species which incorporates carbon dioxide into C4 dicarboxylic acids as the primary...
Psr1, a nuclear localized protein that regulates phosphorus metabolism in Chlamydomonas
Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke
Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing aquatic ecosystems in which phosphorus can be...
Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1
Shimogawara, Kosuke, Wykoff, Dennis D., Usuda, Hideaki, Grossman, Arthur R.
P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling...
Inhibition of Photosynthetic Carbon Metabolism in Isolated Chloroplasts by Iodoacetol Phosphate 12
Usuda, Hideaki, Edwards, Gerald E.
Carbon dioxide-dependent and 3-phosphoglycerate (PGA)-dependent O2 evolution by isolated chloroplasts of wheat is inhibited by micromolar levels of iodoacetol phosphate (IAP). Loss of the activity is...
Usuda, Hideaki, Edwards, Gerald E.
Intact chloroplasts of wheat (Triticum aestivum) were isolated from mesophyll protoplasts. With decreasing concentrations of bicarbonate from 10 to 0.3 millimolar (pH 8.0), the optimal concentration...
Muto, Shoshi, Miyachi, Shigetoh, Usuda, Hideaki, Edwards, Gerald E., Bassham, James A.
Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the...
Localization of Glycerate Kinase and Some Enzymes for Sucrose Synthesis in C3 and C4 Plants 1
Usuda, Hideaki, Edwards, Gerald E.
The localization of some key enzymes leading to sucrose synthesis in photosynthetic tissue of C3 and C4 species was investigated. These included UDP-glucose (UDPG) pyrophosphorylase, sucrose...
Nonaqueous Purification of Maize Mesophyll Chloroplasts 1
A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were...
Recently, a nonaqueous fractionation method of obtaining highly purified mesophyll chloroplasts from maize leaves was established. This method is now used to determine adenine nucleotide levels, the...
The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in an attached leaf of maize (Zea mays L) were measured over a range of intercellular...
Kalt-Torres, Willy, Kerr, Phillip S., Usuda, Hideaki, Huber, Steven C.
Diurnal changes in photosynthetic parameters and enzyme activities were characterized in greenhouse grown maize plants (Zea mays L. cv Pioneer 3184). Rates of net photosynthesis and assimilate export...
Usuda, Hideaki, Kalt-Torres, Willy, Kerr, Phillip S., Huber, Steven C.
Diurnal changes in the regulatory metabolite, fructose-2,6-bisphosphate (F26BP), and key metabolic intermediates of sucrose biosynthesis were studied in maize (Zea mays L. cv Pioneer 3184) during a...
The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in attached leaves of maize (Zea mays L.) were measured over a range of light intensity...
Usuda, Hideaki, Ku, Maurice S. B., Edwards, Gerald E.
The activity and extent of light activation of three photosynthetic enzymes, pyruvate,Pi dikinase, NADP-malate dehydrogenase (NADP-MDH), and fructose 1,6-bisphosphatase (FBPase), were examined in...
Changes in the level of metabolites of the C4 cycle and reductive pentose phosphate (RPP) pathway were measured simultaneously with induction of photosynthesis in maize (Zea mays L.) to evaluate what...
Winter, Klaus, Usuda, Hideaki, Tsuzuki, Mikio, Schmitt, Mark, Edwards, Gerald E., Thomas, Richard J., ...
The leaf anatomy and certain photosynthetic properties of nitrate- and ammonia-grown plants of Moricandia arvensis (L.) DC., a species previously reported to be a C3-C4 intermediate, were...
Usuda, Hideaki, Shimogawara, Kousuke
The effects of low concentrations of phosphate (low-P) on soluble protein content, the activities of 12 different enzymes, and the rates of photosynthesis and respiration on the basis of leaf area...
Psr1, a nuclear localized protein that regulates phosphorus metabolism in Chlamydomonas
Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke
Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing aquatic ecosystems in which phosphorus can be...
Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1
Shimogawara, Kosuke, Wykoff, Dennis D., Usuda, Hideaki, Grossman, Arthur R.
P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling...
Inhibition of Photosynthetic Carbon Metabolism in Isolated Chloroplasts by Iodoacetol Phosphate 12
Usuda, Hideaki, Edwards, Gerald E.
Carbon dioxide-dependent and 3-phosphoglycerate (PGA)-dependent O2 evolution by isolated chloroplasts of wheat is inhibited by micromolar levels of iodoacetol phosphate (IAP). Loss of the activity is...
Usuda, Hideaki, Edwards, Gerald E.
Intact chloroplasts of wheat (Triticum aestivum) were isolated from mesophyll protoplasts. With decreasing concentrations of bicarbonate from 10 to 0.3 millimolar (pH 8.0), the optimal concentration...
Muto, Shoshi, Miyachi, Shigetoh, Usuda, Hideaki, Edwards, Gerald E., Bassham, James A.
Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the...
Localization of Glycerate Kinase and Some Enzymes for Sucrose Synthesis in C3 and C4 Plants 1
Usuda, Hideaki, Edwards, Gerald E.
The localization of some key enzymes leading to sucrose synthesis in photosynthetic tissue of C3 and C4 species was investigated. These included UDP-glucose (UDPG) pyrophosphorylase, sucrose...
Nonaqueous Purification of Maize Mesophyll Chloroplasts 1
A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were...
Recently, a nonaqueous fractionation method of obtaining highly purified mesophyll chloroplasts from maize leaves was established. This method is now used to determine adenine nucleotide levels, the...
The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in an attached leaf of maize (Zea mays L) were measured over a range of intercellular...
Kalt-Torres, Willy, Kerr, Phillip S., Usuda, Hideaki, Huber, Steven C.
Diurnal changes in photosynthetic parameters and enzyme activities were characterized in greenhouse grown maize plants (Zea mays L. cv Pioneer 3184). Rates of net photosynthesis and assimilate export...
Usuda, Hideaki, Kalt-Torres, Willy, Kerr, Phillip S., Huber, Steven C.
Diurnal changes in the regulatory metabolite, fructose-2,6-bisphosphate (F26BP), and key metabolic intermediates of sucrose biosynthesis were studied in maize (Zea mays L. cv Pioneer 3184) during a...
The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in attached leaves of maize (Zea mays L.) were measured over a range of light intensity...
Usuda, Hideaki, Ku, Maurice S. B., Edwards, Gerald E.
The activity and extent of light activation of three photosynthetic enzymes, pyruvate,Pi dikinase, NADP-malate dehydrogenase (NADP-MDH), and fructose 1,6-bisphosphatase (FBPase), were examined in...
Changes in the level of metabolites of the C4 cycle and reductive pentose phosphate (RPP) pathway were measured simultaneously with induction of photosynthesis in maize (Zea mays L.) to evaluate what...
Winter, Klaus, Usuda, Hideaki, Tsuzuki, Mikio, Schmitt, Mark, Edwards, Gerald E., Thomas, Richard J., ...
The leaf anatomy and certain photosynthetic properties of nitrate- and ammonia-grown plants of Moricandia arvensis (L.) DC., a species previously reported to be a C3-C4 intermediate, were...
Usuda, Hideaki, Shimogawara, Kousuke
The effects of low concentrations of phosphate (low-P) on soluble protein content, the activities of 12 different enzymes, and the rates of photosynthesis and respiration on the basis of leaf area...