NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity (2009)
Kamiya, Hiroyuki, Hori, Mika, Arimori, Takao, Sekiguchi, Mutsuo, Yamagata, Yuriko, Harashima, Hideyoshi
The human NUDT5 protein catalyzes the hydrolysis of 8-hydroxy-dGDP. To examine its substrate specificity, four oxidized deoxyribonucleotides (2-hydroxy-dADP, 8-hydroxy-dADP, 5-formyl-dUDP, and...
Takada, Akitsugu, Kamiya, Hiroyuki, Shuto, Satoshi, Matsuda, Akira, Harashima, Hideyoshi
The efficacy of an antitumor nucleoside, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (3'-ethynylcytidine, ECyd), was analyzed in vitro and in vivo. The in vivo antitumor effect of ECyd...
Kamiya, Hiroyuki, Fukunaga, Satoki, Ohyama, Takashi, Harashima, Hideyoshi
The intranuclear disposition of plasmid DNA is highly important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on transgene expression were...
Kamiya, Hiroyuki, Suzuki, Akihiro, Yamaguchi, Yuki, Handa, Hiroshi, Harashima, Hideyoshi
Oxidized RNA precursors formed in the nucleotide pool may be incorporated into RNA. In this study, the incorporation of 8-hydroxyguanosine 5'-triphosphate (8-OH-GTP; 8-oxo-7,8-dihydroguanosine...
Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery (2009)
Higashi, Tomoko, Khalil, Ikramy A., Maiti, Kaustabh K., Lee, Woo Sirl, Akita, Hidetaka, Harashima, Hideyoshi, ...
In this study, we investigated the possible use of novel lipidated sorbitol-based transporters as functional devices for the improvement of non-viral gene delivery. These transporters are composed of...
Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery (2009)
Higashi, Tomoko, Khalil, Ikramy A., Maiti, Kaustabh K., Lee, Woo Sirl, Akita, Hidetaka, Harashima, Hideyoshi, ...
In this study, we investigated the possible use of novel lipidated sorbitol-based transporters as functional devices for the improvement of non-viral gene delivery. These transporters are composed of...
Homhuan, Atthachai, Kogure, Kentaro, Nakamura, Takashi, Shastri, Nilabh, Harashima, Hideyoshi
To improve uptake and cross-presentation of exogenous antigens (Ag) by dendritic cells (DCs), octaarginine-modified liposomes (R8-Lip) were used as a novel strategy for protein-Ag transduction....
Involvement of specialized DNA polymerases in mutagenesis by 8-hydroxy-dGTP in human cells (2009)
Satou, Kazuya, Hori, Mika, Kawai, Kazuaki, Kasai, Hiroshi, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using human 293T cells. Shuttle plasmid DNA containing the supF gene was first...
Sakurai, Yu, Hatakeyama, Hiroto, Akita, Hidetaka, Oishi, Motoi, Nagasaki, Yukio, Futaki, Shiro, ...
We recently developed a multifunctional envelope-type nano device (MEND) for efficient nucleic acid delivery. Here, we report on the development of an octaarigine (R8)-modified MEND encapsulating...
El-Sayed, Ayman, Masuda, Tomoya, Khalil, Ikramy, Akita, Hidetaka, Harashima, Hideyoshi
An octaarginine-modified multifunctional envelope-type nano device (R8-MEND) was previously reported to be an efficient nonviral vector for the delivery of plasmid DNA, in vitro and after topical...
Hatakeyama, Hiroto, Itho, Erika, Akita, Hidetaka, Oishi, Motoi, Nagasaki, Yukio, Futaki, Shiroh, ...
Previously, we developed a multifunctional envelope-type nano device (MEND) for efficient delivery of both pDNA and siRNA. Modification of a MEND with polyuethylene glycol, i.e., PEGylation, is a...
Roles of specialized DNA polymerases in mutagenesis by oxidized guanine (2009)
Kamiya, Hiroyuki, Satou, Kazuya, Hori, Mika, Yamaguchi, Ayaka, Harashima, Hideyoshi
To assess the involvement of specialized DNA polymerases in the mutagenesis induced by an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP,...
Kamiya, Hiroyuki, Uchiyama, Masayuki, Nakatsu, Yoshimichi, Tsuzuki, Teruhisa, Harashima, Hideyoshi
The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg–EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported....
Yamada, Yuma, Akita, Hidetaka, Kamiya, Hiroyuki, Kogure, Kentaro, Yamamoto, Takenori, Shinohara, Yasuo, ...
Mitochondria are the principal producers of energy in higher cells. Mitochondrial dysfunction is implicated in a variety of human diseases, including cancer and neurodegenerative disorders. Effective...
Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP (2008)
Suzuki, Tetsuya, Yamamoto, Kazuo, Harashima, Hideyoshi, Kamiya, Hiroyuki
To examine whether base excision repair suppresses mutations induced by oxidized deoxyribonucleotide 5′-triphosphates in the nucleotide pool, 8-hydroxy-dGTP (8-OH-dGTP) and 2-hydroxy-dATP were...
Kamiya, Hiroyuki, Uchiyama, Masayuki, Nakatsu, Yoshimichi, Tsuzuki, Teruhisa, Harashima, Hideyoshi
The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg–EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported....
UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides (2007)
Hori, Mika, Ishiguro, Chieko, Suzuki, Tetsuya, Nakagawa, Noriko, Nunoshiba, Tatsuo, Kuramitsu, Seiki, ...
Oxidatively damaged DNA precursors (deoxyribonucleotides) are formed by reactive oxygen species. After the damaged DNA precursors are incorporated into DNA, they might be removed by DNA repair...
Kogure, Kentaro, Akita, Hidetaka, Harashima, Hideyoshi
In this review, a new concept called “Programmed Packaging” is proposed for developing non-viral gene delivery systems. A Multifunctional Envelope-type Nano Device (MEND) was developed based on...
Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription. (2007)
Kamiya, Hiroyuki, Suzuki, Akihiro, Kawai, Kazuaki, Kasai, Hiroshi, Harashima, Hideyoshi
Oxidation of RNA precursors may disturb genetic information. In this study, the effects of oxidized RNA precursors on in vitro transcription were examined. Two oxidized ribonucleoside triphosphates,...
Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription. (2007)
Kamiya, Hiroyuki, Suzuki, Akihiro, Kawai, Kazuaki, Kasai, Hiroshi, Harashima, Hideyoshi
Oxidation of RNA precursors may disturb genetic information. In this study, the effects of oxidized RNA precursors on in vitro transcription were examined. Two oxidized ribonucleoside triphosphates,...
Ochiai, Hiroshi, Fujimuro, Masahiro, Yokosawa, Hideyoshi, Harashima, Hideyoshi, Kamiya, Hiroyuki
The intranuclear disposition of exogenous DNA is quite important for the therapeutic effects of the administered DNA. The expression efficiency from one copy of exogenous DNA delivered by...
Octaarginine-modified multifunctional envelope-type nano device for siRNA (2007)
Nakamura, Yoshio, Kogure, Kentaro, Futaki, Shiroh, Harashima, Hideyoshi
The multifunctional envelope-type nano device (MEND) is a novel non-viral gene delivery system for plasmid DNA (pDNA) and oligodeoxynucleotides (ODN). We showed previously that octaarginine-modified...
Kamiya, Hiroyuki, Iida, Emiko, Harashima, Hideyoshi
The Escherichia coli Orf135 (NudG) protein, a MutT-type enzyme, catalyzes the hydrolysis of 2-hydroxy-dATP and 8-hydroxy-dGTP, and its deficiency causes an increase in the mutation frequency. In this...
Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells (2007)
Satou, Kazuya, Kawai, Kazuaki, Kasai, Hiroshi, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by...
Kamiya, Hiroyuki, Fukunaga, Satoki, Ohyama, Takashi, Harashima, Hideyoshi
The intranuclear disposition of a plasmid is extremely important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on plasmid expression were...
Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells (2007)
Satou, Kazuya, Kawai, Kazuaki, Kasai, Hiroshi, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by...
Kamiya, Hiroyuki, Fukunaga, Satoki, Ohyama, Takashi, Harashima, Hideyoshi
The intranuclear disposition of a plasmid is extremely important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on plasmid expression were...
Hama, Susumu, Akita, Hidetaka, Iida, Shinya, Mizuguchi, Hiroyuki, Harashima, Hideyoshi
Quantitative and mechanism-based information on differences in transfection efficiency between viral and non-viral vectors would be highly useful for improving the effectiveness of non-viral vectors....
Hayashi, Yasuhiro, Iida, Shinya, Sato, Yuichiro, Nakaya, Akihiro, Sawada, Akihiro, Kaji, Noritada, ...
In a previous study, we hypothesized that some type 2 diabetes mellitus susceptible genes may be up/downregulated in white blood cells (WBC) of Otsuka Long-Evans Tokushima Fatty (OLETF) rats,...
Induction of Various Mutations during PCRs with Manganese and 8-Hydroxy-dGTP (2007)
Kamiya, Hiroyuki, Ito, Mana, Harashima, Hideyoshi
To induce various mutations randomly, PCRs with Mn2+ and with a mutagenic deoxyribonucleotide, 8-hydroxy-dGTP (8-OH-dGTP), were performed. Mutations were induced by deoxyribonucleotide imbalance plus...
Hayashi, Yasuhiro, Iida, Shinya, Sato, Yuichiro, Nakaya, Akihiro, Sawada, Akihiro, Kaji, Noritada, ...
In a previous study, we hypothesized that some type 2 diabetes mellitus susceptible genes may be up/downregulated in white blood cells (WBC) of Otsuka Long-Evans Tokushima Fatty (OLETF) rats,...
Induction of Various Mutations during PCRs with Manganese and 8-Hydroxy-dGTP (2007)
Kamiya, Hiroyuki, Ito, Mana, Harashima, Hideyoshi
To induce various mutations randomly, PCRs with Mn2+ and with a mutagenic deoxyribonucleotide, 8-hydroxy-dGTP (8-OH-dGTP), were performed. Mutations were induced by deoxyribonucleotide imbalance plus...
Akita, Hidetaka, Ito, Rie, Kamiya, Hiroyuki, Kogure, Kentaro, Harashima, Hideyoshi
Background: Heterogeneity of transgene expression, the presence or absence (below the limit of detection) of transgene expression on a cell-by-cell basis, is a severe disadvantage in the use of...
Akita, Hidetaka, Ito, Rie, Kamiya, Hiroyuki, Kogure, Kentaro, Harashima, Hideyoshi
Background: Heterogeneity of transgene expression, the presence or absence (below the limit of detection) of transgene expression on a cell-by-cell basis, is a severe disadvantage in the use of...
Hama, Susumu, Akita, Hidetaka, Iida, Shinya, Mizuguchi, Hiroyuki, Harashima, Hideyoshi
Quantitative and mechanism-based information on differences in transfection efficiency between viral and non-viral vectors would be highly useful for improving the effectiveness of non-viral vectors....
Hama, Susumu, Akita, Hidetaka, Iida, Shinya, Mizuguchi, Hiroyuki, Harashima, Hideyoshi
Quantitative and mechanism-based information on differences in transfection efficiency between viral and non-viral vectors would be highly useful for improving the effectiveness of non-viral vectors....
Suzuki, Tetsuya, Yamamoto, Kazuo, Harashima, Hideyoshi, Kamiya, Hiroyuki
To examine whether base excision repair suppresses mutations induced by oxidized deoxyribonucleotide 5'-triphosphates in the nucleotide pool, 8-hydroxy-dGTP (8-OH-dGTP) was introduced into...
Hama, Susumu, Akita, Hidetaka, Iida, Shinya, Mizuguchi, Hiroyuki, Harashima, Hideyoshi
Quantitative and mechanism-based information on differences in transfection efficiency between viral and non-viral vectors would be highly useful for improving the effectiveness of non-viral vectors....
Iida, Shinya, Sato, Yuichiro, Nakaya, Akihiro, Shinohara, Yasuo, Hayashi, Yasuhiro, Sawada, Akihiro, ...
In a prior study, we reported on a significant decrease in calpain10 gene expression in white blood cells (WBC) as well as the major insulin-target tissues including liver and adipose tissue, before...
Iida, Shinya, Sato, Yuichiro, Nakaya, Akihiro, Shinohara, Yasuo, Hayashi, Yasuhiro, Sawada, Akihiro, ...
In a prior study, we reported on a significant decrease in calpain10 gene expression in white blood cells (WBC) as well as the major insulin-target tissues including liver and adipose tissue, before...
Mutagenic properties of oxidized GTP and ATP in in vitro transcription-reverse transcription (2006)
Kamiya, Hiroyuki, Suzuki, Akihiro, Kawai, Kazuaki, Kasai, Hiroshi, Harashima, Hideyoshi
Oxidation of RNA precursors may disturb genetic information by mispair formations. In this study, effects of oxidized ribonucleoside triphosphates on in vitro transcription catalyzed by T7 RNA...
Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme (2006)
Hori, Mika, Asanuma, Taketoshi, Inanami, Osamu, Kuwabara, Mikinori, Harashima, Hideyoshi, Kamiya, Hiroyuki
The Escherichia coli Orf17 (NtpA, NudB) protein, a MutT-type enzyme, hydrolyzes oxidized deoxyribonucleotides, including 8-hydroxy-2'-deoxyadenosine 5'-triphosphate and 8-hydroxy-2'-deoxyguanosine...
Silencing of Exogenous DNA in Cultured Cells (2006)
Ochiai, Hiroshi, Harashima, Hideyoshi, Kamiya, Hiroyuki
The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA. Naked luciferase-plasmid DNA was transfected into cultured cells including HeLa...
Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme (2006)
Hori, Mika, Asanuma, Taketoshi, Inanami, Osamu, Kuwabara, Mikinori, Harashima, Hideyoshi, Kamiya, Hiroyuki
The Escherichia coli Orf17 (NtpA, NudB) protein, a MutT-type enzyme, hydrolyzes oxidized deoxyribonucleotides, including 8-hydroxy-2'-deoxyadenosine 5'-triphosphate and 8-hydroxy-2'-deoxyguanosine...
Silencing of Exogenous DNA in Cultured Cells (2006)
Ochiai, Hiroshi, Harashima, Hideyoshi, Kamiya, Hiroyuki
The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA. Naked luciferase-plasmid DNA was transfected into cultured cells including HeLa...
Nakamura, Yoshio, Kogure, Kentaro, Yamada, Yuma, Futaki, Shiroh, Harashima, Hideyoshi, 小暮, 健太朗
A multifunctional envelope-type nano device (MEND) was developed for use as an efficient non-viral system for the delivery of plasmid DNA (pDNA) using octaarginine (R8) as an internalizing ligand....
Nakamura, Yoshio, Kogure, Kentaro, Yamada, Yuma, Futaki, Shiroh, Harashima, Hideyoshi
A multifunctional envelope-type nano device (MEND) was developed for use as an efficient non-viral system for the delivery of plasmid DNA (pDNA) using octaarginine (R8) as an internalizing ligand....
Nakamura, Yoshio, Kogure, Kentaro, Yamada, Yuma, Futaki, Shiroh, Harashima, Hideyoshi
A multifunctional envelope-type nano device (MEND) was developed for use as an efficient non-viral system for the delivery of plasmid DNA (pDNA) using octaarginine (R8) as an internalizing ligand....
Kamiya, Hiroyuki, Cadena-Amaro, Claudio, Dugué, Laurence, Yakushiji, Hiroyuki, Minakawa, Noriaki, Matsuda, Akira, ...
The MTH1 protein catalyzes hydrolysis of oxidatively damaged purine nucleotides including 8-hydroxy-dGTP to the monophosphates. The MTH1 protein seems to act as an important defense system against...
Kamiya, Hiroyuki, Cadena-Amaro, Claudio, Dugué, Laurence, Yakushiji, Hiroyuki, Minakawa, Noriaki, Matsuda, Akira, ...
The MTH1 protein catalyzes hydrolysis of oxidatively damaged purine nucleotides including 8-hydroxy-dGTP to the monophosphates. The MTH1 protein seems to act as an important defense system against...
A multifunctional envelope-type nano device for novel gene delivery of siRNA plasmids (2005)
Moriguchi, Rumiko, Kogure, Kentaro, Akita, Hidetaka, Futaki, Shiroh, Miyagishi, Makoto, Taira, Kazunari, ...
A multifunctional envelope-type nano device (MEND) for use in the delivery of siRNA expression plasmids is described. The plasmid DNA encoding anti-luciferase short interfering RNA (siRNA) was...
Hoshika, Shuichi, Minakawa, Noriaki, Kamiya, Hiroyuki, Harashima, Hideyoshi, Matsuda, Akira
Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the Photinus luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured...
Construction of a multifunctional envelope-type nano device by a SUV*-fusion method (2005)
Sasaki, Kentaro, Kogure, Kentaro, Chaki, Sinji, Kihira, Yoshitaka, Ueno, Masaharu, Harashima, Hideyoshi
In this review, a new concept called “Programmed Packaging” is proposed for developing non-viral gene delivery systems. A Multifunctional Envelope-type Nano Device (MEND) was developed based on...
New NTP analogs: the synthesis of 4'-thioUTP and 4'-thioCTP and their utility for SELEX (2005)
Kato, Yuka, Minakawa, Noriaki, Komatsu, Yasuo, Kamiya, Hiroyuki, Ogawa, Naoki, Harashima, Hideyoshi, ...
Nucleic Acids Research (c) Oxford University Press 2004. All rights reserved.
Tsuchiya, Hiroyuki, Sawamura, Tomoko, Uchiyama, Masayuki, Harashima, Hideyoshi, Kamiya, Hiroyuki
The correction of a mutated gene is a highly attractive approach for gene therapy. This in vivo mutagenesis method will also be an effective tool in biotechnology. However, the current small fragment...
New NTP analogs: the synthesis of 4'-thioUTP and 4'-thioCTP and their utility for SELEX (2005)
Kato, Yuka, Minakawa, Noriaki, Komatsu, Yasuo, Kamiya, Hiroyuki, Ogawa, Naoki, Harashima, Hideyoshi, ...
The synthesis of the triphosphates of 4′-thiouridine and 4′-thiocytidine, 4′-thioUTP (7; thioUTP) and 4′-thioCTP (8; thioCTP), and their utility for SELEX (systematic evolution of ligands by...
Kogure, Kentaro, Moriguchi, Rumiko, Sasaki, Kentaro, Ueno, Masaharu, Futaki, Shiroh, Harashima, Hideyoshi
The previous termdevelopment of a multifunctional envelope-type nano devicenext term (MEND) for use in previous terma non-viralnext term gene delivery system using previous terma novel lipid film...
Fisher, David I, Cartwright, Jared L, Harashima, Hideyoshi, Kamiya, Hiroyuki, McLennan, Alexander G
Abstract Background Nudix hydrolases form a protein family whose function is to hydrolyse intracellular nucleotides and so regulate their levels and eliminate potentially toxic derivatives. The...
Kamiya, Hiroyuki, Ishiguro, Chieko, Harashima, Hideyoshi
The Escherichia coli MutT protein hydrolyzes 8-hydroxy-dGTP (8-OH-dGTP) in vitro, and mutT gene deficiencies cause increased spontaneous A:T→C:G mutations. However, no direct evidence exists for...
Kamiya, Hiroyuki, Satou, Kazuya, Hori, Mika, Iida, Emiko, Ishiguro, Chieko, Harashima, Hideyoshi
Oxidized deoxyribonucleotides, 2-hydroxydeoxyadenosine 5′-triphosphaie (2-OH-dATP) and 8-hydroxydeoxyguanosine 5′-triphosphate (8-OH-dGTP), were introduced into Escherichia coli strains deficient...
Utilization efficiency of the oxidized purine nucleotide analogs by DNA polymerase {eta} (2003)
Nishimoto, Naomi, Kamiya, Hiroyuki, Harashima, Hideyoshi, Izuta, Shunji
To elucidate the behavior of DNA polymerase η against the oxidized purine nucleotides, we determined the utilization efficiency of 2-hydroxy-dATP and 8-hydroxy-dGTP by the recombinant yeast DNA...
Mutations induced by 2-hydroxy-dATP during in vitro replication with a HeLa extract (2003)
Satou, Kazuya, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of oxidized dATP, 2-hydroxydeoxyadenosine 5′-triphosphate (2-OH-dATP), was examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. 2-OH-dA TP...
Satou, Kazuya, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of an oxidized form of dATP, 2‐hydroxydeoxyadenosine 5′‐triphosphate (2‐OH‐dATP), was examined using an SV40 origin‐dependent in vitro replication system...
Utilization efficiency of the oxidized purine nucleotide analogs by DNA polymerase {eta} (2003)
Nishimoto, Naomi, Kamiya, Hiroyuki, Harashima, Hideyoshi, Izuta, Shunji
Kamiya, Hiroyuki, Tsuchiya, Hiroyuki, Karino, Naoko, Ueno, Yoshihito, Matsuda, Akira, Harashima, Hideyoshi
To examine the mutagenicity of 5-formylcytosine (5-fC), an oxidation product of 5-meth-ylcytosine (5-mC), 5-fC was incorporated into predetermined sites of double-stranded shuttle vectors. The...
Substrate recognition by the human MTH1 protein (2002)
Kamiya, Hiroyuki, Dugué, Laurence, Yakushiji, Hiroyuki, Pochet, Sylvie, Nakabeppu, Yusaku, Harashima, Hideyoshi
Substrate recognition by the human MTH1 protein (2002)
Kamiya, Hiroyuki, Dugué, Laurence, Yakushiji, Hiroyuki, Pochet, Sylvie, Nakabeppu, Yusaku, Harashima, Hideyoshi
A nucleotide pool sanitizing enzyme, the human MIH1 protein, hydrolyses 2-hydroxy-dATP, 8-hydroxy-dATP, and 8-hydroxy-dGTP. To examine the substrate recognition mechanism of the MTH1 protein, ten...
Satou, Kazuya, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of an oxidized form of dATP, 2-hydroxydeoxyadenosine 5′-triphosphate (2-OH-dATP), was examined using an SV40 origin-dependent in vitro replication system with a HeLa extract....
New NTP analogs: the synthesis of 4′-thioUTP and 4′-thioCTP and their utility for SELEX
Kato, Yuka, Minakawa, Noriaki, Komatsu, Yasuo, Kamiya, Hiroyuki, Ogawa, Naoki, Harashima, Hideyoshi, ...
The synthesis of the triphosphates of 4′-thiouridine and 4′-thiocytidine, 4′-thioUTP (7; thioUTP) and 4′-thioCTP (8; thioCTP), and their utility for SELEX (systematic evolution of ligands by...
Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerases
Shimizu, Masatomi, Gruz, Petr, Kamiya, Hiroyuki, Kim, Su-Ryang, Pisani, Francesca M., Masutani, Chikahide, ...
Deranged oxidative metabolism is a property of many tumour cells. Oxidation of the deoxynucleotide triphosphate (dNTP) pool, as well as DNA, is a major cause of genome instability. Here, we report...
Yamada, Masami, Nunoshiba, Tatsuo, Shimizu, Masatomi, Gruz, Petr, Kamiya, Hiroyuki, Harashima, Hideyoshi, ...
Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP opposite template guanine or thymine and 8-hydroxy-dGTP exclusively opposite adenine in vitro. Mutator phenotypes in sod/fur strains...
Satou, Kazuya, Harashima, Hideyoshi, Kamiya, Hiroyuki
The mutagenicity of an oxidized form of dATP, 2-hydroxydeoxyadenosine 5′-triphosphate (2-OH-dATP), was examined using an SV40 origin-dependent in vitro replication system with a HeLa extract....
New NTP analogs: the synthesis of 4′-thioUTP and 4′-thioCTP and their utility for SELEX
Kato, Yuka, Minakawa, Noriaki, Komatsu, Yasuo, Kamiya, Hiroyuki, Ogawa, Naoki, Harashima, Hideyoshi, ...
The synthesis of the triphosphates of 4′-thiouridine and 4′-thiocytidine, 4′-thioUTP (7; thioUTP) and 4′-thioCTP (8; thioCTP), and their utility for SELEX (systematic evolution of ligands by...
Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerases
Shimizu, Masatomi, Gruz, Petr, Kamiya, Hiroyuki, Kim, Su-Ryang, Pisani, Francesca M., Masutani, Chikahide, ...
Deranged oxidative metabolism is a property of many tumour cells. Oxidation of the deoxynucleotide triphosphate (dNTP) pool, as well as DNA, is a major cause of genome instability. Here, we report...
Yamada, Masami, Nunoshiba, Tatsuo, Shimizu, Masatomi, Gruz, Petr, Kamiya, Hiroyuki, Harashima, Hideyoshi, ...
Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP opposite template guanine or thymine and 8-hydroxy-dGTP exclusively opposite adenine in vitro. Mutator phenotypes in sod/fur strains...
Hama, Susumu, Akita, Hidetaka, Iida, Shinya, Mizuguchi, Hiroyuki, Harashima, Hideyoshi
Quantitative and mechanism-based information on differences in transfection efficiency between viral and non-viral vectors would be highly useful for improving the effectiveness of non-viral vectors....