Chaudhuri, R.R., Peters, S.E., Pleasance, S.J., Northen, H., Willers, C., Paterson, G.K., ...
Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of...
Xu, W., Liu, L., Charles, I.G., Moncada, S.
Nitric oxide (NO) is a pleiotropic signalling molecule that binds to cytochrome c oxidase (complex IV) reversibly and in competition with oxygen1, 2, 3. This action of NO has both physiological and...
Temporal expression of inducible nitric oxide synthase in mouse and human placenta (1999)
Baylis, S.A., Sandra, A., Russell, R.J., Rijhsinghani, A., Charles, I.G., ...
The aim of this study is to investigate the changes in expression and activity of inducible nitric oxide synthase (iNOS) in the developing murine embryo and mouse and human placenta. Using reverse...
Structure of Bordetella pertussis virulence factor P.69 pertactin (1996)
Emsley, P., Charles, I.G., Fairweather, N.F., Isaacs, N.W.
A new generation of whooping-cough vaccines contain P.69 pertactin, a surface-exposed domain of an outer membrane protein expressed by the virulent bacterium Bordetella pertussis. This protein is a...
High level heterologous expression in E.coli using the anaerobically-activated nirB promoter (1991)
Oxer, M.D., Bentley, C.M., Doyle, J.G., Peakman, T.C., Charles, I.G., Makoff, A.J.
The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at...
Boyd, A.C., Charles, I.G., Keyte, J.W., Brammar, W.J.
A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus nethylotrophus The enzyme was shown to have the non recognition sequence 5′ - T C C Pu A C...
Structure of Bordetella pertussis virulence factor P.69 pertactin
Emsley, P., Charles, I.G., Fairweather, N.F., Isaacs, N.W.
A new generation of whooping-cough vaccines contain P.69 pertactin, a surface-exposed domain of an outer membrane protein expressed by the virulent bacterium Bordetella pertussis. This protein is a...
Roles of nitric oxide in tumor growth.
Jenkins, D C, Charles, I G, Thomsen, L L, Moss, D W, Holmes, L S, Baylis, S A, ...
A subclone of the human colon adenocarcinoma cell line DLD-1, which grew reproducibly as subcutaneous tumors in nude mice, was isolated. Such cells, when engineered to generate nitric oxide (NO)...
Induction of calcium-dependent nitric oxide synthases by sex hormones.
Weiner, C P, Lizasoain, I, Baylis, S A, Knowles, R G, Charles, I G, Moncada, S
We have examined the effects of pregnancy and sex hormones on calcium-dependent and calcium-independent nitric oxide synthases (NOSs) in the guinea pig. Pregnancy (near term) caused a > 4-fold...
Charles, I G, Palmer, R M, Hickery, M S, Bayliss, M T, Chubb, A P, Hall, V S, ...
Incubation of human articular chondrocytes with interleukin 1 beta results in the time-dependent expression of nitric oxide (NO) synthase. We report here the isolation of a cDNA clone which encodes a...
Leininger, E, Roberts, M, Kenimer, J G, Charles, I G, Fairweather, N, Novotny, P, ...
A 69-kDa protein has been identified on the surface of the Gram-negative pathogen Bordetella pertussis that can elicit a protective immune response in animal models. This protein is associated with...
Charles, I G, Harford, S, Brookfield, J F, Shaw, W V
Proteus mirabilis PM13 is a well-characterized chloramphenicol-sensitive isolate which spontaneously gives rise to resistant colonies on solid media containing chloramphenicol (50 micrograms ml-1) at...
Charles, I G, Keyte, J W, Shaw, W V
In Proteus mirabilis PM13 chloramphenicol resistance is mediated by the cat gene, a single copy of which is present in both resistant and sensitive isolates and which reverts at a high frequency. RNA...
Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system.
Charles, I G, Rodgers, B C, Makoff, A J, Chatfield, S N, Slater, D E, Fairweather, N F
The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was...
Charles, I G, Dougan, G, Pickard, D, Chatfield, S, Smith, M, Novotny, P, ...
Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein...
Boyd, A C, Charles, I G, Keyte, J W, Brammar, W J
A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C...
Charles, I G, Keyte, J W, Brammar, W J, Hawkins, A R
The nucleotide sequence of a 1.9 Kb HindIII fragment of DNA derived from the arom locus of A.nidulans and encoding the biosynthetic dehydroquinase activity has been determined. The sequences encoding...
High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.
Oxer, M D, Bentley, C M, Doyle, J G, Peakman, T C, Charles, I G, Makoff, A J
The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at...
The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.
Charles, I G, Keyte, J W, Brammar, W J, Smith, M, Hawkins, A R
The AROM locus of A. nidulans, which governs five consecutive steps in pre-chorismate aromatic amino acid biosynthesis, has been cloned in a bacteriophage vector. The nucleotide sequence of the locus...
Van Den Hombergh, J P, Moore, J D, Charles, I G, Hawkins, A R
The pentafunctional AROM protein of Aspergillus nidulans is encoded by the complex aromA locus and catalyses steps 2-6 in the synthesis of chorismate, the common precursor for the aromatic amino...
Moore, J D, Lamb, H K, Garbe, T, Servos, S, Dougan, G, Charles, I G, ...
The aroQ gene of Mycobacterium tuberculosis, encoding a type-II 3-dehydroquinase, and the aroD gene of Salmonella typhi, encoding a type-I 3-dehydroquinase, have been highly overexpressed in...
Characterization of the type I dehydroquinase from Salmonella typhi.
Moore, J D, Hawkins, A R, Charles, I G, Deka, R, Coggins, J R, Cooper, A, ...
The type I dehydroquinase from the human pathogen Salmonella typhi was overexpressed in an Escherichia coli host and purified to homogeneity. The S. typhi enzyme was characterized in terms of its...
Boyhan, A, Smith, D, Charles, I G, Saqi, M, Lowe, P N
Nitric oxide synthase (EC 1.14.13.39) catalyses the conversion of arginine, NADPH and oxygen to nitric oxide and citrulline, using haem, (6R)-5,6,7,8-tetrahydro-l-biopterin (tetrahydrobiopterin),...
Cubberley, R R, Alderton, W K, Boyhan, A, Charles, I G, Lowe, P N, Old, R W
Nitric oxide synthase (EC 1.14.13.39) is a homodimer. Limited proteolysis has previously shown that it consists of two major domains. The C-terminal or reductase domain binds FMN, FAD and NADPH. The...
Leiper, J M, Santa Maria, J, Chubb, A, MacAllister, R J, Charles, I G, Whitley, G S, ...
Methylarginines inhibit nitric oxide synthases (NOS). Cellular concentrations of methylarginines are determined in part by the activity of dimethylarginine dimethylaminohydrolase (DDAH; EC 3.5.3....
Roles of nitric oxide in tumor growth.
Jenkins, D C, Charles, I G, Thomsen, L L, Moss, D W, Holmes, L S, Baylis, S A, ...
A subclone of the human colon adenocarcinoma cell line DLD-1, which grew reproducibly as subcutaneous tumors in nude mice, was isolated. Such cells, when engineered to generate nitric oxide (NO)...
Induction of calcium-dependent nitric oxide synthases by sex hormones.
Weiner, C P, Lizasoain, I, Baylis, S A, Knowles, R G, Charles, I G, Moncada, S
We have examined the effects of pregnancy and sex hormones on calcium-dependent and calcium-independent nitric oxide synthases (NOSs) in the guinea pig. Pregnancy (near term) caused a > 4-fold...
Charles, I G, Palmer, R M, Hickery, M S, Bayliss, M T, Chubb, A P, Hall, V S, ...
Incubation of human articular chondrocytes with interleukin 1 beta results in the time-dependent expression of nitric oxide (NO) synthase. We report here the isolation of a cDNA clone which encodes a...
Leininger, E, Roberts, M, Kenimer, J G, Charles, I G, Fairweather, N, Novotny, P, ...
A 69-kDa protein has been identified on the surface of the Gram-negative pathogen Bordetella pertussis that can elicit a protective immune response in animal models. This protein is associated with...
Charles, I G, Harford, S, Brookfield, J F, Shaw, W V
Proteus mirabilis PM13 is a well-characterized chloramphenicol-sensitive isolate which spontaneously gives rise to resistant colonies on solid media containing chloramphenicol (50 micrograms ml-1) at...
Charles, I G, Keyte, J W, Shaw, W V
In Proteus mirabilis PM13 chloramphenicol resistance is mediated by the cat gene, a single copy of which is present in both resistant and sensitive isolates and which reverts at a high frequency. RNA...
Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system.
Charles, I G, Rodgers, B C, Makoff, A J, Chatfield, S N, Slater, D E, Fairweather, N F
The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was...
Charles, I G, Dougan, G, Pickard, D, Chatfield, S, Smith, M, Novotny, P, ...
Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein...
Boyd, A C, Charles, I G, Keyte, J W, Brammar, W J
A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C...
Charles, I G, Keyte, J W, Brammar, W J, Hawkins, A R
The nucleotide sequence of a 1.9 Kb HindIII fragment of DNA derived from the arom locus of A.nidulans and encoding the biosynthetic dehydroquinase activity has been determined. The sequences encoding...
High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.
Oxer, M D, Bentley, C M, Doyle, J G, Peakman, T C, Charles, I G, Makoff, A J
The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at...
The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.
Charles, I G, Keyte, J W, Brammar, W J, Smith, M, Hawkins, A R
The AROM locus of A. nidulans, which governs five consecutive steps in pre-chorismate aromatic amino acid biosynthesis, has been cloned in a bacteriophage vector. The nucleotide sequence of the locus...
Van Den Hombergh, J P, Moore, J D, Charles, I G, Hawkins, A R
The pentafunctional AROM protein of Aspergillus nidulans is encoded by the complex aromA locus and catalyses steps 2-6 in the synthesis of chorismate, the common precursor for the aromatic amino...
Moore, J D, Lamb, H K, Garbe, T, Servos, S, Dougan, G, Charles, I G, ...
The aroQ gene of Mycobacterium tuberculosis, encoding a type-II 3-dehydroquinase, and the aroD gene of Salmonella typhi, encoding a type-I 3-dehydroquinase, have been highly overexpressed in...
Characterization of the type I dehydroquinase from Salmonella typhi.
Moore, J D, Hawkins, A R, Charles, I G, Deka, R, Coggins, J R, Cooper, A, ...
The type I dehydroquinase from the human pathogen Salmonella typhi was overexpressed in an Escherichia coli host and purified to homogeneity. The S. typhi enzyme was characterized in terms of its...
Boyhan, A, Smith, D, Charles, I G, Saqi, M, Lowe, P N
Nitric oxide synthase (EC 1.14.13.39) catalyses the conversion of arginine, NADPH and oxygen to nitric oxide and citrulline, using haem, (6R)-5,6,7,8-tetrahydro-l-biopterin (tetrahydrobiopterin),...
Cubberley, R R, Alderton, W K, Boyhan, A, Charles, I G, Lowe, P N, Old, R W
Nitric oxide synthase (EC 1.14.13.39) is a homodimer. Limited proteolysis has previously shown that it consists of two major domains. The C-terminal or reductase domain binds FMN, FAD and NADPH. The...
Leiper, J M, Santa Maria, J, Chubb, A, MacAllister, R J, Charles, I G, Whitley, G S, ...
Methylarginines inhibit nitric oxide synthases (NOS). Cellular concentrations of methylarginines are determined in part by the activity of dimethylarginine dimethylaminohydrolase (DDAH; EC 3.5.3....
Structure of Bordetella pertussis virulence factor P.69 pertactin
Emsley, P., Charles, I.G., Fairweather, N.F., Isaacs, N.W.
A new generation of whooping-cough vaccines contain P.69 pertactin, a surface-exposed domain of an outer membrane protein expressed by the virulent bacterium Bordetella pertussis. This protein is a...
Jenkins, D. C., Charles, I. G., Baylis, S. A., Lelchuk, R., Radomski, M. W., Moncada, S.
A panel of human colonic adenocarcinoma cell lines was examined both for expression of mRNAs of the nitric oxide synthase (NOS) gene family and for evidence of enzymic activity based on citrulline...
Nichols, C. E., Johnson, C., Lamb, H. K., Lockyer, M., Charles, I. G., Hawkins, A. R., ...
The X-ray crystal structure of the GTPase YjeQ from S. typhimurium is presented and compared with those of orthologues from T. maritima and B. subtilis.