Ron, Izhar, Sepunaro, Lior, Izhakov, Stella, Friedman, Noga, Pecht, Israel, Sheves, Mordechai, ...
Electron transfer (ET) through proteins, a fundamental element of many biochemical reactions, has been studied intensively in solution. We report the results of electron transport (ETp) measurements...
Péterfy, Hajna, Tóth, Gábor, Pecht, Israel, Erdei, Anna
A peptide with the natural sequence derived from the complement component C3a, designated C3a7, and C3a9, having a modified sequence of that, was previously shown to inhibit the high-affinity IgER...
Bruder, Dunja, Nussbaum, Alexander K., Gakamsky, Dimitry M., Schirle, Markus, Stevanovic, Stefan, Singh-Jasuja, Harpreet, ...
Immunodominance in CD8+ T cell responses against Listeria monocytogenes is a well-recognized but still not fully understood phenomenon. From listeriolysin, the major virulence factor of L....
CD8 kinetically promotes ligand binding to the T-cell antigen receptor (2005)
Gakamsky, Dmitry M., Luescher, Immanuel F., Pramanik, Aladdin, Kopito, Ronen B., Lemonnier, Francois, Vogel, Horst, ...
The mechanism of CD8 cooperation with the TCR in antigen recognition was studied on live T cells. Fluorescence correlation measurements yielded evidence of the presence of two TCR and CD8...
Bruder, Dunja, Nussbaum, Alexander K., Gakamsky, Dimitry M., Schirle, Markus, Stevanovic, Stefan, Singh-Jasuja, Harpreet, ...
Immunodominance in CD8+ T cell responses against Listeria monocytogenes is a well-recognized but still not fully understood phenomenon. From listeriolysin, the major virulence factor of L....
Role of transmembrane domains in the functions of B- and T-cell receptors (1998)
Raphael Zidovetzki, Burkhard Rost, Israel Pecht
The antigen receptors on the surface of B- and T-lymphocytes are complexes of several integral membrane proteins, essential for their proper expression and function. Recent studies demonstrated that...
Complement peptide C3a inhibits IgEmediated triggering of rat mucosal mast cells (1995)
Erdei, Anna, Andreev, Sergey, Pecht, Israel, Eichmann, K.
The relationship between mast cells' secretory response to stimulation via their type 1 Fcϵ receptors (FcϵRI) and that provided by the C3a fragment of the complement system was...
Interaction of a human Fc{gamma}RIIb1 (CD32) isoform with murine and human IgG subclasses (1993)
Warmerdam, Petra A. M., Westerdaal, Nomdo A. C., Capel, Peter J. A., ...
A group of Fc receptor molecules, classified CD32, recognize the Fc moiety of IgG with low affinity. We report the Isolation and Identification of different hFcγRllb cDNA clones, amongst which are...
Schneider, Helga, Cohen-Dayag, Anat, Pecht, Israel
Mast cells respond to clustering of the type I FC receptor (FcRI) on their membranes by mediator secretion. Recently, a marked enhancement of tyrosine phosphorylation on several proteins has been...
Ortega, Enrique, Schneider, Helga, Pecht, Israel, Steinman, L.
We have recently described a monoclonal antibody, mAb G63, which identifies a novel membrane component of mast cells. This antigen is a glycoprotein with an apparent molecular mass of 28–40 kd, and...
Phosphorylation of type II Fc{gamma} receptor on activated human B lymphocytes (1990)
Sarmay, Gabriella, Pecht, Israel, Gergely, Janos, Ben-Neriah, Y.
Activation of resting human B lymphocytes either by cross-linking their membranal IgM or by phorbol esters has been previously demonstrated to modulate the type II receptor for Fcγ domains (FcγRII)...
Margalit, Ruth, Kostic, Nenad M., Che, Chi-Ming, Blair, David F., Chiang, Huey-Jenn, Pecht, Israel, ...
The reaction between a5RuH2O2+ (a is NH3) and Pseudomonas aeruginosa azurin at pH 7, followed by oxidation, yields a5Ru(His-83)3+-azurin(Cu2+) as the major product. Spectroscopic measurements...
Enhanced rate of intramolecular electron transfer in an engineered purple CuA azurin
Farver, Ole, Lu, Yi, Ang, Majorie C., Pecht, Israel
The recent expression of an azurin mutant where the blue type 1 copper site is replaced by the purple CuA site of Paracoccus denitrificans cytochrome c oxidase has yielded an optimal system for...
Deuterium isotope effect on the intramolecular electron transfer in Pseudomonas aeruginosa azurin
Farver, Ole, Zhang, Jingdong, Chi, Qijin, Pecht, Israel, Ulstrup, Jens
Intramolecular electron transfer in azurin in water and deuterium oxide has been studied over a broad temperature range. The kinetic deuterium isotope effect, kH/kD, is smaller than unity (0.7 at 298...
Allosteric control of internal electron transfer in cytochrome cd1 nitrite reductase
Farver, Ole, Kroneck, Peter M. H., Zumft, Walter G., Pecht, Israel
Cytochrome cd1 nitrite reductase is a bifunctional multiheme enzyme catalyzing the one-electron reduction of nitrite to nitric oxide and the four-electron reduction of dioxygen to water. Kinetics and...
Subnanosecond motions of tryptophan residues in proteins
Munro, Ian, Pecht, Israel, Stryer, Lubert
The dynamics of protein molecules in the subnanosecond and nanosecond time range were investigated by time-resolved fluorescence polarization spectroscopy. Synchrotron radiation from a storage ring...
The reaction of horse-heart cytochrome c with hydrated electrons has been studied by the pulseradiolysis technique. In neutral solution, the ferriheme group was reduced in a bimolecular reaction that...
T cell receptor-ligand interactions: A conformational preequilibrium or an induced fit
Gakamsky, Dmitry M., Luescher, Immanuel F., Pecht, Israel
Kinetic parameters of T cell receptor (TCR) interactions with its ligand have been proposed to control T cell activation. Analysis of kinetic data obtained has so far produced conflicting insights;...
Fluorescence Enhancement of Laccase Induced by Reduction of Cu(II) Sites
Goldberg, Michel, Pecht, Israel
The intrinsic fluorescence of laccase (p-diphenol:O2 oxidoreductase, EC 1.10.3.2), emitted by its tyrosinyl and tryptophanyl residues, underwent significant enhancement upon reduction of the enzyme...
CD8 Kinetically Promotes Ligand Binding to the T-Cell Antigen Receptor
Gakamsky, Dmitry M., Luescher, Immanuel F., Pramanik, Aladdin, Kopito, Ronen B., Lemonnier, François, Vogel, Horst, ...
The mechanism of CD8 cooperation with the TCR in antigen recognition was studied on live T cells. Fluorescence correlation measurements yielded evidence of the presence of two TCR and CD8...
Enhanced rate of intramolecular electron transfer in an engineered purple CuA azurin
Farver, Ole, Lu, Yi, Ang, Majorie C., Pecht, Israel
The recent expression of an azurin mutant where the blue type 1 copper site is replaced by the purple CuA site of Paracoccus denitrificans cytochrome c oxidase has yielded an optimal system for...
Deuterium isotope effect on the intramolecular electron transfer in Pseudomonas aeruginosa azurin
Farver, Ole, Zhang, Jingdong, Chi, Qijin, Pecht, Israel, Ulstrup, Jens
Intramolecular electron transfer in azurin in water and deuterium oxide has been studied over a broad temperature range. The kinetic deuterium isotope effect, kH/kD, is smaller than unity (0.7 at 298...
Allosteric control of internal electron transfer in cytochrome cd1 nitrite reductase
Farver, Ole, Kroneck, Peter M. H., Zumft, Walter G., Pecht, Israel
Cytochrome cd1 nitrite reductase is a bifunctional multiheme enzyme catalyzing the one-electron reduction of nitrite to nitric oxide and the four-electron reduction of dioxygen to water. Kinetics and...
Subnanosecond motions of tryptophan residues in proteins
Munro, Ian, Pecht, Israel, Stryer, Lubert
The dynamics of protein molecules in the subnanosecond and nanosecond time range were investigated by time-resolved fluorescence polarization spectroscopy. Synchrotron radiation from a storage ring...
The reaction of horse-heart cytochrome c with hydrated electrons has been studied by the pulseradiolysis technique. In neutral solution, the ferriheme group was reduced in a bimolecular reaction that...
T cell receptor-ligand interactions: A conformational preequilibrium or an induced fit
Gakamsky, Dmitry M., Luescher, Immanuel F., Pecht, Israel
Kinetic parameters of T cell receptor (TCR) interactions with its ligand have been proposed to control T cell activation. Analysis of kinetic data obtained has so far produced conflicting insights;...
Fluorescence Enhancement of Laccase Induced by Reduction of Cu(II) Sites
Goldberg, Michel, Pecht, Israel
The intrinsic fluorescence of laccase (p-diphenol:O2 oxidoreductase, EC 1.10.3.2), emitted by its tyrosinyl and tryptophanyl residues, underwent significant enhancement upon reduction of the enzyme...
CD8 Kinetically Promotes Ligand Binding to the T-Cell Antigen Receptor
Gakamsky, Dmitry M., Luescher, Immanuel F., Pramanik, Aladdin, Kopito, Ronen B., Lemonnier, François, Vogel, Horst, ...
The mechanism of CD8 cooperation with the TCR in antigen recognition was studied on live T cells. Fluorescence correlation measurements yielded evidence of the presence of two TCR and CD8...
Selective inhibition of the FcɛRI-induced de novo synthesis of mediators by an inhibitory receptor
Abramson, Jakub, Licht, Arieh, Pecht, Israel
Aggregation of the type 1 Fcɛ receptors (FcɛRI) on mast cells initiates a network of biochemical processes culminating in secretion of both granule-stored and de novo-synthesized inflammatory...
Farver, Ole, Grell, Ernst, Ludwig, Bernd, Michel, Hartmut, Pecht, Israel
Intramolecular electron transfer between CuA and heme a in solubilized bacterial (Paracoccus denitrificans) cytochrome c oxidase was investigated by pulse radiolysis. CuA, the initial electron...
Kinetic evidence for a ligand-binding-induced conformational transition in the T cell receptor
Gakamsky, Dmitry M., Lewitzki, Erwin, Grell, Ernst, Saulquin, Xavier, Malissen, Bernard, Montero-Julian, Felix, ...
Thermodynamics and kinetics of the interaction between T cell receptor specific for cytomegalovirus peptide (TCRCMV) and its specific ligand, pp65–HLA-A*0201 complex, were studied by surface...