J. A. Teixeira

Details der Publikationsliste

Zeitraum

1993 - 2009

Anzahl

378

Co-Autoren

Effect of moderate electric fields in the permeation properties of chitosan coatings (2009)

Souza, B. W. S., Cerqueira, M. A., Casariego, A., Lima, A. M. P., Teixeira, J. A., Vicente, A. A.

Edible films and coatings can provide additional protection for food, while being a fully biodegradable, environmentally friendly packaging system. Preliminary works have shown that the presence of a...

Partitioning of glycomacropeptide in aqueous two-phase systems (2009)

Silva, C. A. S., Rojas, Edwin Elard Garcia, Teixeira, J. A.

The partition behavior of glycomacropeptide (GMP) was determined in polyethylene glycol (PEG) and sodium citrate aqueous two-phase systems (ATPS). It was found that the partitioning of GMP depends on...

Physical properties of edible coatings and films made with a polysaccharide from Anacardium occidentale L. (2009)

Cerqueira, Miguel A., Souza, Bartolomeu W.S., Souza, Marthyna P., Teixeira, J. A., Vicente, A. A.

The effect of the concentrations of the polysaccharide from Anacardium occidentale L. (Policaju) and a surfactant (Tween 80) on relevant properties of edible coatings/films, in view of their...

Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens (2009)

Roriz, Margarida S., Osma, Johann F., Teixeira, J. A., Rodríguez Couto, S.

Response surface methodology (RSM) was applied to optimise the decolouration of the diazo dye Reactive Black 5 (RB5) by crude laccase from the white-rot fungus Trametes pubescens. The presence of the...

Oxygen mass transfer to emulsions in a bubble column contactor (2009)

Gómez Díaz, D., Gomes, Nelma, Teixeira, J. A., Belo, Isabel

Present work deals with oxygen mass transfer in an oil-in-water biphasic medium, employed in the production of γ-decalactone by the biotransformation of methyl ricinoleate. A bubble column contactor...

Rheological and structural characterization of gels from whey protein hydrolysates/locust bean gum mixed systems (2009)

Rocha, C., Teixeira, J. A., Hilliou, L., Sampaio, Paula, Gonçalves, M. P.

The gelling ability of whey proteins can be changed by limited hydrolysis and by the addition of other components such as polysaccharides. In this work the effect of the concentration of locust bean...

Chitosan/clay films' properties as affected by biopolymer and clay micro/nanoparticles' concentrations (2009)

Casariego, A., Souza, B. W. S., Cerqueira, M. A., Teixeira, J. A., Cruz, L., Díaz, R., ...

Blends of chitosan (from Cuban lobster) and clay micro/nanoparticles were prepared by dispersion of the clay particles in the film matrix and the films obtained were characterized in terms of water...

Suitability of novel galactomannans as edible coatings for tropical fruits (2009)

Cerqueira, M. A., Lima, A. M. P., Teixeira, J. A., Moreira, R. A., Vicente, A. A.

The main objective of this work was to determine the optimal composition of edible coatings in view of their application to extend the shelf life of several tropical fruits. Coatings constituted by...

Retention of Cr(VI) and Pb(II) on a loamy sand soil : kinetics, equilibria and breakthrough (2009)

Fonseca, Bruna, Maio, H., Quintelas, C., Teixeira, J. A., Tavares, Teresa

Illegal discharges are of great concern among industry activities, since they occur under uncontrolled conditions. In most cases, effluents are acidic and the concentrations of heavy metals are very...

Gas-liquid interfacial area in oxygen absorption into oil-in-water emulsions (2009)

Gómez Díaz, D., Gomes, Nelma, Teixeira, J. A., Belo, Isabel

Present work includes an exhaustive study about gas-liquid interfacial area between gas phase and liquid heterogeneous medium generated in an airlift bioreactor. The model system studied is composed...

Effect of solids on gas-liquid mass transfer (2009)

Costa, J., Ferreira, A., Rocha, F. A., Teixeira, J. A.

The effect of the solids on the mass transfer characteristics in a bubble column was studied experimentally for the systems air/water/Polyvinyl chloride (PVC) and air/water/glass beads. Volumetric...

Oxygen effect in γ-decalactone production through biotransformation of ricinoleic acid (2009)

Gomes, Nelma, Teixeira, J. A., Belo, Isabel

γ-Decalactone can be produced biotechnologically from the degradation of ricinoleic acid by yeasts, namely Yarrowia lipolytica. Preliminary studies using two different ricinoleic acid sources...

Exploitation of agro industrial wastes as immobilization carrier for solid-state fermentation (2009)

Orzua, María C., Mussatto, Solange I., Contreras-Esquivel, Juan C., Rodriguez, Raul, Garza, Heliodoro De La, Teixeira, J. A., ...

Ten agro industrial wastes were assessed for their suitability as fungus immobilization carrier for solid-state fermentation (SSF). The wastes included creosote bush leaves (Larrea tridentata),...

Fructooligosaccharides and β-fructofuranosidase production by Aspergillus japonicus immobilized on lignocellulosic materials (2009)

Mussatto, Solange I., Aguilar Gonzalez, Cristobal Noé, Rodrigues, L. R., Teixeira, J. A.

This work describes the fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) production from sucrose (200 g/l) by Aspergillus japonicus ATCC 20236 immobilized on different lignocellulosic...

β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus (2009)

Mussatto, Solange I., Rodrigues, L. R., Teixeira, J. A.

The fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of β-fructofuranosidases (FFase). The...

Stability of clavulanic acid under variable pH, ionic strength and temperature conditions : a new kinetic approach (2009)

Santos, Valéria Carvalho, Pereira, Jorge F. Brandão, Haga, Raquel Brandão, Rangel-Yagui, Carlota O., Teixeira, J. A., Converti, Attilio, ...

Clavulanic acid (CA) is a β-lactam antibiotic that alone exhibits only weak antibacterial activity, but is a potent inhibitor of β-lactamases enzymes. For this reason it is used as a therapeutic in...

Production of 3-hydroxy-γ-decalactone, the precursor of two decenolides with flavouring properties, by the yeast Yarrowia lipolytica (2009)

Escamilla Garcia, Erandi, Aguedo, Mário, Gomes, Nelma, Choquet, Armelle, Belo, Isabel, Teixeira, J. A., ...

3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia...

Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides (2009)

Mussatto, Solange I., Aguilar Gonzalez, Cristobal Noé, Rodrigues, L. R., Teixeira, J. A.

The ability of Aspergillus japonicus ATCC 20236 to colonize different synthetic materials (polyurethane foam, stainless steel sponge, vegetal fiber, pumice stones, zeolites, and foam glass) and to...

The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect (2009)

Lopes, Marlene, Araújo, Carla, Aguedo, Mário, Gomes, Nelma, Gonçalves, Cristiana, Teixeira, J. A., ...

BACKGROUND: The aim of this work was to study the ability of two different wild type strains of the yeast Yarrowia lipolytica to grow on olive mill wastewater (OMW) and their potential to produce...

Isolation of a seed coagulant Moringa oleifera lectin (2009)

Santos, Andréa F. S., Luz, Luciana A., Argolo, Adriana C. C., Teixeira, J. A., Paiva, Patrícia M. G.

In this work hemagglutinating activity (HA) was investigated in distinct Moringa oleifera tissue extracts. A new lectin from seeds (cMoL) was purified and characterized; hemagglutinating and...

Liquid–liquid equilibrium of the Ucon 50-HB5100/sodium citrate aqueous two-phase systems (2009)

Tubio, Gisela, Nerli, Bibiana B., Picó, Guillermo A., Venâncio, Armando, Teixeira, J. A.

The phase diagrams of Ucon 50-HB5100/sodium citrate aqueous two-phase systems were determined at 5, 20 and 40 ◦C. Two medium pHs 5.20 and 8.20 were assayed. The binodal curves were satisfactorily...

Extraction, purification and characterization of galactomannans from non-traditional sources (2009)

Cerqueira, M. A., Pinheiro, Ana Cristina Dias, Souza, B. W. S., Lima, A. M. P., Ribeiro, Clara, Miranda, Cândida, ...

This work presents a methodology for the extraction of galactomannans from seeds of four different species of Leguminosae (Adenanthera pavonina, Caesalpinia pulcherrima, Gleditsia triacanthos and...

Functional polysaccharides as edible coatings for cheese (2009)

Cerqueira, M. A., Lima, A. M. P., Souza, B. W. S., Teixeira, J. A., Moreira, R. A., Vicente, A. A.

The objective of the present study was to apply the polysaccharides from different nontraditional sources for cheese coatings. Chitosan, galactomannan from Gleditsia triacanthos, and agar from...

Galacto-oligosaccharides production during lactose hydrolysis by free Aspergillus oryzae β-galactosidase and immobilized on magnetic polysiloxane-polyvinyl alcohol (2009)

Neri, D. F. M., Balcão, V. M., Costa, Rafael S., Rocha, I., Ferreira, E. C., Torres, D., ...

The synthesis of galacto-oligosaccharides (GOS) by the action of Aspergillus oryzae β-galactosidase free and immobilized on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) was studied. A maximum...

Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles (2009)

Neri, D. F. M., Balcão, V. M., Dourado, Fernando, Oliveira, J. M., Carvalho Junior, L. B., Teixeira, J. A.

Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this...

Significance of osteopontin expression in human invasive breast tumour stroma (2009)

Rodrigues, L. R., Lopes, Nair, Sousa, Bárbara, Vieira, Daniella, Milanezi, Fernanda, Paulsson, Marie, ...

Osteopontin has been reported to stimulate cell adhesion, migration and specific signalling functions. Its overexpression has been found in melanoma, breast, lung, colorectal, stomach and ovarian...

Lactoferrin and cancer disease prevention (2009)

Rodrigues, L. R., Teixeira, J. A., Schmitt, Fernando C., Paulsson, Marie, Lindmark-Mässon, Helena

Lactoferrin (LF) is an iron-binding glycoprotein that is composed of the transferrin family and is predominantly found in the products of the exocrine glands located in the gateways of the digestive,...

Análise da fermentação em bioreatores cilindrocônicos de bancada na elaboração de cerveja com adjunto de banana (2009)

Matos, Gislaine Fernandes De, Teixeira, J. A., Silva, Daniel Pereira Da, Vicente, A. A., ...

O aumento do extrato inicial de fermentação e uso de adjuntos na substituição do malte vem sendo uma alternativa para diminuição de custos na produção de cervejas. Uma outra característica...

Application of wheat straw autohydrolysis liquor to xylanase and β-xylosidase large-scale production in a stirred tank bioreactor (2009)

Michelin, Michele, Silva, Daniel Pereira Da, Ruzene, Denise S., Vicente, A. A., Jorge, João A., ...

Research into microbial xylanases production has increased due to its several applications. In this context, studies that make this practice feasible are important. Wheat bran is an inexpensive...

Avaliação do processo de bioprodução de etanol a partir de xilose pela levedura Pichia Stipitis NRRL Y-7124 em reator de bancada (2009)

Silva, João Paulo A., Mussatto, Solange I., Roberto, Inês Conceição, Teixeira, J. A.

A produção de etanol pela levedura Pichia stipitis foi avaliada em reator de bancada sob diferentes condições de aeração e agitação em meio semi-sintético contendo xilose (90,0 g/L) como...

Exploiting the sequence of naturally occurring elastin : construction, production and characterization of a recombinant thermoplastic protein-based polymer (2009)

Machado, Raul, Ribeiro, A. J., Padrão, Jorge, Silva, D., Nobre, A., Teixeira, J. A., ...

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A...

Moderate electric fields can inactivate Escherichia coli at room temperature (2009)

Machado, L., Pereira, Ricardo, Martins, Rui C., Teixeira, J. A., Vicente, A. A.

The inactivation of Escherichia coli using moderate electric fields (MEF) below 25 °C, was investigated. Keeping the temperature always below 25 °C demonstrated that electric fields are involved in...

Comparison of adsorption equilibrium of fructose, glucose and sucrose on potassium gel-type and macroporous sodium ion-exchange resins (2009)

Nobre, C., Santos, Maria João, Dominguez, Ana, Torres, D., Rocha, Orlando, Peres, A. M., ...

Adsorption equilibrium of fructose, glucose and sucrose was evaluated on sulfonated poly(styrene-co-divinylbenzene) cation-exchange resins. Two types of resins were used: potassium (K+) gel-type and...

Comparative transcriptome analysis between original and evolved recombinant lactose-consuming Saccharomyces cerevisiae strains (2008)

Guimarães, Pedro M. R., Le Berre, V., Sokol, Serguei, François, J., Teixeira, J. A., Domingues, Lucília

The engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A...

Fermentation of high concentrations of lactose to ethanol by engineered flocculent saccharomyces cerevisiae (2008)

Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília

The development of microorganims that efficiently ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous bio-ethanol production....

Starch analysis using hydrodynamic chromatography with a mixed-bed particle column (2008)

Dias, Ricardo P., Fernandes, C. S., Mota, M., Teixeira, J. A., Yelshin, Alexander

Columns packed with commercial glass beads 5 and 19 lm average size and a mixture of both (0.7 volume fraction of large particles) were used to analyse starch composition by hydrodynamic...

Optimization of ethanol production from cheese whey powder by Kluyveromyces fragilis using factorial design and response surface methodology (2008)

Dragone, Giuliano, Mussatto, Solange I., Teixeira, J. A.

The individual and combined effects of initial lactose concentration, temperature and inoculum concentration on ethanol production from cheese whey powder by Kluyveromyces fragilis were investigated....

Identification and evaluation of fungal strains with fucoidan degradation potential (2008)

Rodríguez Jasso, Rosa María, Aguilar Gonzalez, Cristobal Noé, Pastrana, Lorenzo, Teixeira, J. A.

Ten fungal strains isolated of Mexican semi-desert were screened for ability to hydrolyze fucoidan in order to search microorganism capable to produce sulfated fucans-degrading enzymes. Plate assay...

Hydrothermal treatments of corn cob and hemicelluloses extraction (2008)

Ruzene, Denise S., Yagiz, Elif, Marangoz, Dilek, Silva, Daniel Pereira Da, Vicente, A. A., Teixeira, J. A.

Corn cob samples were treated with water (autohydrolysis reaction) using a liquid to solid ratio of 10:1 w/w. The optimal condition for extraction of hemicelluloses was found at 185ºC for 30 min....

A new strategy for xylanase production using wheat straw autohydrolysis liquor as substrate (2008)

Michelin, Michele, Ruzene, Denise S., Silva, Daniel Pereira Da, Vicente, A. A., Jorge, João A., ...

Agro-industrial residues are lignocellulosic materials with a high content of cellulose, hemicellulose and lignin. If such residues can be produced in bioprocesses (e.g. xylanase production) there is...

Relative hydrophobicity of (PEG or Ucon)-salt ATPSs (2008)

Silvério, Sara C., Madeira, Pedro, Rodríguez, Oscar, Teixeira, J. A., Macedo, Eugénia

Aqueous Two-Phase Systems (ATPSs) are biphasic systems composed mainly by water. ATPSs are obtained upon mixing of two aqueous solutions of certain polymers or a polymer and a salt (above certain...

Euler-euler simulation of gas-liquid and gas-liquid-solid airlift reactors : case study (2008)

Simcik, M., Havlica, J., Ruzicka, M. C., Drahoš, J., Teixeira, J. A., Brányik, Tomáš

The Czech Science Foundation / Grantová agentura České republiky (GACR) - GA104/07/1110, GA104/06/1418.

Development of bioprocesses based in biphasic media involving the yeast Yarrowia lipolytica (2008)

Gomes, Nelma, Gonçalves, Cristiana, Lopes, Marlene, Aguedo, Mário, Teixeira, J. A., Mota, M., ...

Yarrowia lipolytica has been intensively used in our research group to develop different bioprocesses based in the ability of this yeast to use hydrophobic substrates. Work has been focused in the...

Computational shelf-life dating : complex systems approaches to food quality and safety (2008)

Martins, Rui C., Lopes, Vítor V., Vicente, A. A., Teixeira, J. A.

Shelf-life is defined as the time that a product is acceptable and meets the consumers expectations regarding food quality. It is the result of the conjunction of all services in production,...

Fructooligosaccharides production using immobilized cells of Aspergillus japonicus (2008)

Mussatto, Solange I., Dragone, Giuliano, Rodrigues, L. R., Teixeira, J. A.

The fructooligosaccharides (FOS) production using immobilized cells of the fungus Aspergillus japonicus ATCC 20236 was evaluated. Polyurethane foam, stainless steel sponge, vegetal fiber sponge,...

A new strategy for using banana as an ingredient in the brewing process (2008)

Carvalho, Giovani B. M., Silva, Daniel Pereira Da, Vicente, A. A., Teixeira, J. A.

Beer is a traditionally fermented beverage made from malted grains of barley, hops, yeast, and water, while banana is an important food crop cultivated widely in tropical and subtropical areas and is...

Influence of the concentration of locust bean gum on the gelling ability of whey peptic hydrolysates (2008)

Rocha, Cristina, Hilliou, Loïc, Teixeira, J. A., Gonçalves, M. P.

The gelling ability of whey proteins can be changed by limited hydrolysis and by the presence of other components such as polysaccharides; depending on the environmental conditions it can either be...

Lactose fermentation by recombinant Saccharomyces cerevisiae strains (2008)

Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília

The development of Saccharomyces cerevisiae strains with the ability to ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous...

β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports: A comparative experimental and modelling study of the galactooligosaccharides production (2008)

Neri, D. F. M., Balcão, V. M., Costa, Rafael S., Ferreira, E. C., Torres, D., Rodrigues, Lígia M., ...

β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several...

Measurement of gas phase characteristics using amonofibre optical probe in a three-phase flow (2008)

Mena, P. C., Rocha, F. A., Teixeira, J. A., Sechet, P., Cartellier, C.

The study of gas–liquid–solid systems structure requires reliable measurement tools. In this paper, preliminary results on the potential use of a monofibre optical probe to investigate such flow...

Immobilization of b-galactosidase from kluyveromyces lactis onto a polysiloxane–polyvinyl alcohol magnetic (mPOS–PVA) composite for lactose hydrolysis (2008)

Neri, D. F. M., Balcão, V. M., Carvalho Junior, L. B., Teixeira, J. A.

β-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS–PVA, using glutaraldehyde as activating agent and its properties were evaluated. The enzymatic water insoluble...

Expression of frutalin, an α-d-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris (2008)

Felix, W., Moreira, R. A., Teixeira, J. A., Domingues, Lucília

Frutalin is an α-d-galactose-binding lectin expressed in breadfruit seeds. Its isolation from plant is time-consuming and results in a heterogeneous mixture of different lectin isoforms. In order to...

Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctane reversed micelles (2008)

Nascimento, Cynthia O., Araújo, Regina M. S., Chaves, Maria E. C., Paiva, Patrícia M. G., ...

Crataeva tapia bark lectin was extracted from a crude extract into a reversed micelle phase of the anionic surfactant AOT in isooctane and back-extracted, to a final aqueous phase by addition of...

ΔG(CH2) in PEG-Salt and Ucon-Salt Aqueous Two-Phase Systems (2008)

Silvério, Sara C., Madeira, Pedro, Rodríguez, Oscar, Teixeira, J. A., Macedo, Eugénia

Three new phase diagrams for polymer−salt aqueous two-phase systems (ATPS) at 23 °C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained...

Interactions between bacterial surfaces and milk proteins, impact on food emulsions stability (2008)

Mai Huong Ly, Aguedo, Mário, Goudot, S., Le, M. L., Cayot, P., Teixeira, J. A., ...

Bacteria possess physicochemical surface properties such as hydrophobicity, Lewis acid/base and charge which are involved in physicochemical interactions between cells and interfaces. Moreover, food...

Differentiation of human pre-adipocytes by recombinant adiponectin (2008)

Avides, Maria Do Carmo, Domingues, Lucília, Vicente, A. A., Teixeira, J. A.

Obesity has become a global health problem and it is linked to a higher risk of diseases and metabolic disorders such as diabetes, cardiovascular disease, and cancer. The adipose tissue plays an...

Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems (2008)

Madeira, Pedro, Teixeira, J. A., Macedo, Eugénia, Mikheeva, Larissa M., Zaslavsky, Boris Y.

Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems...

Stimulation of zero-trans rates of lactose and maltose uptake into yeasts by preincubation with hexose to increase the adenylate energy charge (2008)

Guimarães, Pedro M. R., Multanen, Jyri-Pekka, Domingues, Lucília, Teixeira, J. A., Londesborough, John

Initial rates of sugar uptake (zero-trans rates) are often measured by incubating yeast cells with radiolabeled sugars for 5 to 30 s and determining the radioactivity entering the cells. The yeast...

Application of binary packing for starch separation by hydrodynamic chromatography (2008)

Mota, M., Teixeira, J. A., Dias, Ricardo P., Yelshin, Alexander

Columns packed with commercial glass beads of 5 and 19 microns average diameter and binary mixtures with finest fraction of 5 micron (30 % volume fraction of the mixture) were used to analyse starch...

Ethanol production from high-glucose industrial substrates using ethanol-tolerant Saccharomyces cerevisiae strains (2008)

Cunha, M. R. M., Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília

Ethanol is well known as a toxic metabolite for yeast cells. Thus, strains that can grow well under high ethanol stress condition are highly desirable. This work aims to select and characterize...

Nisin biotechnological production : evaluation and perspectives in the development of new applications (2008)

Jozala, A. F., Silva, D. P., Vicente, A. A., Pessoa Júnior, Adalberto, Penna, T. C. V., Teixeira, J. A.

Nisin is a commercially available bacteriocin of the lantibiotic group produced by Lactococcus lactis ATCC 11454. The importance of nisin as an effective preservative is the fact that its spectrum of...

Alcoholic beverage from cheese whey : identification of volatile compounds (2008)

Dragone, Giuliano, Mussatto, Solange I., Oliveira, J. M., Teixeira, J. A.

A spirit was produced from cheese whey continuous fermentation by Kluyveromyces marxianus, and the volatile compounds present in this alcoholic drink were identified. Fermentation was performed in a...

Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity (2008)

Oliveira, M. D. L., Andrade, C. A. S., Magalhães, N. S., Teixeira, J. A., Cunha, M. G. C., ...

Aims: The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds. Methods and Results: The E. uniflora lectin (EuniSL) was isolated from the...

ΔG(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systems (2008)

Madeira, Pedro, Teixeira, J. A., Macedo, Eugénia, Mikheeva, Larissa M., Zaslavsky, Boris Y.

Phase diagrams were determined for aqueous two-phase systems (ATPSs) formed by different paired combinations of Dextran (Dex-75), Ficoll-70, polyethylene glycol (PEG-8000), hydroxypropyl starch...

Adaptive evolution of a lactose-consuming saccharomyces cerevisiae recombinant (2008)

Guimarães, P., François, J., Parrou, J. L., Teixeira, J. A., Domingues, Lucília

The construction of Saccharomyces cerevisiae strains that ferment lactose has biotechnological interest, particularly for cheese whey fermentation. A flocculent lactose-consuming S. cerevisiae...

Simulation of liquid phase accumulation for the centrifugal dewatering of activated sludge (2008)

Mota, M., Teixeira, J. A., Abaev, G. N., Yelshyna, I., Yelshin, Alexander

Centrifugation of activated sludge is a frequently used dewatering process but, in spite of this, there is a need for a fitting function for moderate and high sludge concentrations. Liquid phase...

A review of flavour formation in continuous beer fermentations (2008)

Brányik, Tomáš, Vicente, A. A., Dostálek, Pavel, Teixeira, J. A.

The attractive prospect of a continuous beer fermentation system consists mostly of the accelerated transformation of wort into beer. Although continuous beer fermentation has been studied as a...

High gravity batch and continuous processes for beer production : evaluation of fermentation performance and beer quality (2008)

Silva, Daniel Pereira Da, Brányik, Tomáš, Giuliano, Giuliano, Vicente, A. A., Teixeira, J. A.

This study deals with two innovative brewing processes, high gravity batch and complete continuous beer fermentation systems. The results show a significant influence of the variables such as...

Alcoholic fermentation of lactose by engineered flocculent Saccharomyces cerevisiae (2008)

Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília

The construction of Saccharomyces cerevisiae strains with the ability to ferment lactose has biotechnological interest, particularly for cheese whey fermentation to ethanol. Direct fermentation of...

Total reuse of brewer’s spent grain in chemical and biotechnological processes for the production of added-value compounds (2008)

Mussatto, Solange I., Dragone, Giuliano, Teixeira, J. A., Roberto, Inês Conceição

Brewer’s spent grain was fractionated by means of three different procedures: dilute acid hydrolysis, for the hemicellulose recovery; alkaline hydrolysis, for the lignin solubilization, and...

Sistemas computacionais para a previsão da qualidade e segurança alimentar : uma perspectiva de sistemas complexos (2008)

Martins, Rui C., Vicente, A. A., Teixeira, J. A., Lopes, Vítor V.

Os sistemas computacionais para a previsão da qualidade e segurança alimentar assumem hoje maior relevância na prototipagem e simulação da cadeia de distribuição (e.g. gestão do...

Banana as adjunct in beer production : applicability and performance of fermentative parameters (2008)

Carvalho, Giovani B. M., Silva, Daniel Pereira Da, Bento, Camila V., Vicente, A. A., Teixeira, J. A., ...

Traditionally, the raw materials for beer production are barley, hops, water, and yeast, but most brewers use also different adjuncts. During the alcoholic fermentation, the contribution of aroma...

The intensification of gas–liquid flows with a periodic, constricted oscillatory-meso tube (2007)

Reis, N., Mena, P. C., Vicente, A. A., Teixeira, J. A., Rocha, F. A.

The experimental study of gas dispersion in a vertical periodically, constricted, oscillatory meso-tube (OMT) is herein presented. Water was continuously pumped through the OMT in the laminar flow...

Physicochemical characterization of the PEG8000-Na2SO4 aqueous two-phase system (2007)

Rodríguez, Oscar, Silvério, Sara C., Madeira, Pedro, Teixeira, J. A., Macedo, Eugénia

The polyethylene glycol-sodium sulfate aqueous two-phase system has been characterized at 23 °C. Tielines for the phase diagram were obtained experimentally. Phases in equilibrium were characterized...

Alternative flows in a microfluidic device (2007)

Oliveira, Hugo Carneiro, Miranda, J. M., Teixeira, J. A., Vicente, A. A., Correia, J. H., Minas, Graça

The mixing properties of systems combining alternate flows with obstacles are studied by numerical methods. Preliminary results show that the layers of high and low solute concentration, created by...

Novel functional polysaccharides as edible coatings for cheese (2007)

Cerqueira, M. A., Lima, A. M. P., Vicente, A. A., Teixeira, J. A., Moreira, R. A.

One of the problems occurring during cheese ripening and later on, throughout the distribution chain, is the occurrence of molds and the loss of water. Usually, this problem is solved by the use of...

Chitosan coating surface and permeation properties as affected by plasticizer, surfactant and polymer concentration-application to vegetables (2007)

Casariego, A., Souza, B. W. S., Vicente, A. A., Teixeira, J. A., Cruz, L., Díaz, R.

Edible coatings can act as moisture, lipid, and gas barriers and can improve the textural properties of foods or serve as carriers of functional agents such as colors, flavors, antioxidants,...

Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus (2007)

Ribeiro, Orquídea, Gombert, Andreas Karoly, Teixeira, J. A., Domingues, Lucília

The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of...

Oxygen mass transfer in a biphasic medium : influence on the biotransformation of methyl ricinoleate into γ-decalactone by the yeast Yarrowia lipolytica (2007)

Gomes, Nelma, Aguedo, Mário, Teixeira, J. A., Belo, Isabel

In this work, an empirical correlation is proposed to describe kLα as a function of operating conditions (agitation and aeration rates) and of oil and surfactant volumetric fractions in a...

Monitorização da qualidade do leite de cabra durante a pasteurização e processamento óhmico usando sensores de fibra óptica (2007)

Martins, Rui C., Coelho, A. M., Lopes, Vítor V., Pereira, Ricardo, Teixeira, J. A., Vicente, A. A.

Este estudo avalia a viabilidade da espectroscopia UVVIS- SWNIR na degradação da qualidade do leite de cabra durante a pasteurização e aquecimento Ohmico. Foram utilizados: i) leite de cabra cru;...

The bubble size distribution and O2 mass transfer in an oscillating, meso-scale tube (2007)

Reis, N., Mena, P. C., Rocha, F. A., Vicente, A. A., Teixeira, J. A.

This work aims at characterizing bubble size distribution and its control in a small-scale, constricted tube in the presence of an oscillating flow. From previous works, it became clear that the full...

The role of osteopontin in tumour progression and metastasis in breast cancer (2007)

Rodrigues, L. R., Teixeira, J. A., Schmitt, Fernando C., Paulsson, Marie, Lindmark-Mässon, Helena

The use of cancer biomarkers to anticipate the outlines of disease has been an emerging issue, especially as cancer treatment has made such positive steps in the last few years. Progress in the...

Adaptive evolution of a lactose-consuming saccharomyces cerevisiae recombinant (2007)

Guimarães, P., François, J., Parrou, J. L., Teixeira, J. A., Domingues, Lucília

The construction of Saccharomyces cerevisiae strains with the ability to efficiently ferment lactose has biotechnological interest, particularly for the alcoholic fermentation of cheese whey (a high...

Assessment of brewing yeast age based on selective bud scar staining and subsequent fluorescence measurement by flow cytometry (2007)

Lehnert, Radek, Kuřec, Michal, Brányik, Tomáš, Mota, André, Teixeira, J. A.

To study the yeast cell aging in continuous beer fermentation processes has a considerable practical significance. Hence, an expeditious method of yeast age estimation was developed based on...

Strategies for the prevention of microbial biofilm formation on silicone rubber voice prostheses (2007)

Rodrigues, L. R., Banat, Ibrahim M., Teixeira, J. A.

Total laryngectomy, a surgical treatment for extensive cancer of larynx, which alters swallowing and respiration in patients, is followed up with a surgical voice restoration procedure comprising...

The impact of process parameters on flavour profile of alcohol-free beer from a single-stage continuous gas-lift reactor with immobilized yeast (2007)

Lehnert, Radek, Kuřec, Michal, Brányik, Tomáš, Teixeira, J. A.

In order to study the formation and conversion of the most important flavour compounds, the real wort used in alcohol-free beer fermentation was mimicked by a complex model medium containing glucose,...

Optimization of edible coating composition to retard strawberry fruit senescence (2007)

Ribeiro, Clara, Vicente, A. A., Teixeira, J. A., Miranda, Cândida

The ability of polysaccharide-based (starch, carrageenan and chitosan) coatings to extend the shelf-life of strawberry fruit (Fragaria ananassa) were studied, mainly for industrial applications. The...

Permeability and effective thermal conductivity of bisized porous media (2007)

Dias, Ricardo P., Fernandes, Carla S., Mota, M., Teixeira, J. A., Yelshin, Alexander

In the region of minimum porosity of particulate binary mixtures, heat exchange and permeability were found to be higher than the ones obtained with a mono-size packing built with the same small size...

Carboxymethylcellulose obtained by ethanol/water organosolv process under acid conditions (2007)

Ruzene, Denise S., Gonçalves, A. R., Teixeira, J. A.

Sugar cane bagasse pulps were obtained by ethanol/water organosolv process under acid and alkaline conditions. The best condition of acid pulping for the sugarcane bagasse was 0.02 mol/L sulfuric...

Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger β-galactosidase production (2007)

Teixeira, J. A., Lima, Nelson, Silva, Nancy A. Da, Domingues, Lucília

A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast...

Modeling and simulation of fructo-oligosaccharides production (2007)

Rocha, Orlando, Dominguez, Ana, Nobre, C., Torres, D., Rodrigues, L. R., Teixeira, J. A., ...

It is foreseen that Systems Biology will have a great impact not only in Metabolic Engineering and Drug Discovery efforts, but also in Bioprocess development and optimization. In fact, the...

Edible coatings from galactomannans and their application to tropical fruits (2007)

Cerqueira, M. A., Lima, A. M. P., Moreira, H. H. T., Vicente, A. A., Teixeira, J. A., ...

Edible coatings play an important role in the quality and in the resistance to transportation, storage and display of a wide range of fresh and processed foods. Polysaccharide coatings with an...

Preparation of ingredients containing an ACE-inhibitory peptide by tryptic hydrolysis of whey protein concentrates (2007)

Pinho, O., Mota, M. V. T., Tavares, P., Pereira, A., Gonçalves, M. P., ...

This study describes the characterisation of whey protein hydrolysates obtained from tryptic hydrolysis to assess their application as ingredients with angiotensin-converting-enzyme (ACE) inhibitory...

Ohmic heating treatment of fruit puree (2007)

Machado, L., Vicente, A. A., Teixeira, J. A., Miranda, F.

Ohmic heating is an alternative pasteurization process and is growing interest in industry. Understanding the influence of ohmic heating on the sensorial and microbiological quality of foods is a...

Purification of fructo-oligosaccharides by adsorption onto charcoal filters (2007)

Nobre, C., Dominguez, Ana, Torres, D., Rocha, Orlando, Rodrigues, L. R., Rocha, I., ...

Production of fructo-oligosaccharides has received particular attention recently due to its beneficial effects as prebiotics. Fermentative processes appear to be a good technique for large scale...

Comparison of chemical properties of food products processed by conventional and ohmic heating (2007)

Pereira, Ricardo, Pereira, M., Teixeira, J. A., Vicente, A. A.

The effect of ohmic and conventional heat processing of different food products on their chemical and physical parameters was studied. Depending on the food being analysed, parameters such as pH,...

Oxygen mass transfer to emulsions in bubble column contactor (2007)

Gómez Díaz, D., Gomes, Nelma, Teixeira, J. A., Belo, Isabel

Present work includes an exhaustive study about the oxygen absorption process by the analysis of volumetric mass transfer coefficient and the corresponding value of interfacial area between gas phase...

Permeability analysis in bisized porous media : wall effect between particles of different size (2007)

Dias, Ricardo P., Fernandes, Carla S., Teixeira, J. A., Mota, M., Yelshin, Alexander

The permeability of binary packings of glass beads with different size ratio – 13.3, 20, and 26.7, was investigated. In the Kozeny–Carman equation, the dependence of the tortuosity τ on the...

Chitosan coating surface properties as affected by plasticizer, surfactant and polymer concentrations in relation to the surface properties of tomato and carrot (2007)

Casariego, A., Souza, B. W. S., Vicente, A. A., Teixeira, J. A., Cruz, L., Díaz, R.

The objectives of this work were to determine the effects of the concentrations of glycerol and sorbitol (as hydrophilic plasticizers), Tween 80 (as surfactant) and chitosan on the wettability of...

Death kinetics of Escherichia coli in goat milk and Bacillus licheniformis in cloudberry jam treated by ohmic heating (2007)

Pereira, Ricardo, Martins, Joana, Mateus, C., Teixeira, J. A., Vicente, A. A.

The influence of ohmic heating on the death kinetic parameters of Escherichia coli ATCC® 25922 in goat milk and spores of Bacillus licheniformis ATCC® 14580 in cloudberry jam was investigated and...

Continuous immobilized yeast reactor system for complete beer fermentation using spent grains and corncobs as carrier materials (2006)

Brányik, Tomáš, Silva, Daniel Pereira Da, Vicente, A. A., Lehnert, Radek, Dostálek, Pavel, ...

Despite extensive research carried out in the last few decades, continuous beer fermentation has not yet managed to outperform the traditional batch technology. An industrial breakthrough in favour...

Continuous immobilized yeast reactor system for complete beer fermentation using spent grains and corncobs as carrier materials (2006)

Brányik, Tomáš, Silva, Daniel Pereira Da, Vicente, A. A., Lehnert, Radek, Dostálek, Pavel, ...

Despite extensive research carried out in the last few decades, continuous beer fermentation has not yet managed to outperform the traditional batch technology. An industrial breakthrough in favour...

Low-cost fermentative medium for biosurfactant production by probiotic bacteria (2006)

Rodrigues, L. R., Teixeira, J. A.

Potential use of alternative fermentative medium for biosurfactant production by Lactococcus lactis 53 and Streptococcus thermophilus A was studied. Suitable models were established to describe the...

Complex systems challenges in bioengineering modelling and simulation (2006)

Martins, Rui C., Vicente, A. A., Teixeira, J. A.

Bioengineering has a considerable potential for the use of complex systems (CS) in modelling and simulation. Biological systems are built on functional elements, where the large variety of existing...

Proof-of-concept of a novel micro-bioreactor for fast development of industrial bioprocesses (2006)

Reis, N., Gonçalves, C. N., Vicente, A. A., Teixeira, J. A.

The experimental performance of a novel micro-bioreactor envisaged for parallel screening and development of industrial bioprocesses has been tested in this work. The micro-bioreactor with an...

Motion of a magnetic flow follower in two-phase flow : application to the study of airlift reactor hydrodynamics (2006)

Klein, Jaroslav, Godó, Stefan, Teixeira, J. A.

A low-cost and simple magnetic particle tracer method was adapted to characterize the hydrodynamic behavior of an internal- and an external-loop airlift reactor (ALR). The residence time distribution...

Utilisation of controlled pore topology for the separation of bioparticles in a mixed-glass beads column (2006)

Mota, M., Teixeira, J. A., Yelshin, Alexander, Cortez, Susana

To study the flow of shaped particles in porous media, elution of spherical and rod-like micro-organisms was performed through beds of spherical glass beads. A 0.04 cm/s constant flow rate was used...

Oscillatory and alternate flows in a microfluidic device (2006)

Oliveira, Hugo Carneiro, Miranda, J. M., Teixeira, J. A., Vicente, A. A., Correia, J. H., Minas, Graça

The mixing properties of systems with oscillatory or alternate flows are studied by numerical methods. Preliminary results show that, for alternate flows, the contact area between high and low...

Tortuosity variation in a low density binary particulate bed (2006)

Dias, Ricardo P., Teixeira, J. A., Mota, M., Yelshin, Alexander

The importance of particle size ratio and particle composition in the properties of a mixed bed is well known. Nevertheless, the dependence of the bed channel tortuosity T on the porosity ε in the...

Isolation and partial characterization of a biosurfactant produced by streptococcus thermophilus A (2006)

Rodrigues, L. R., Teixeira, J. A., Mei, Henny Van Der

Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the...

Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain (2006)

Juraščík, Martin, Guimarães, P., Klein, Jaroslav, Domingues, Lucília, Teixeira, J. A., Markoš, J.

This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the...

Novas técnicas cromatográficas (2006)

Mota, M., Teixeira, J. A., Yelshin, Alexander, Cortez, Susana

A cromatografia é um processo de separação muito especial, na medida em que permite separar compostos de misturas complexas com grande precisão. Mesmo elementos muito similares, como as...

Ohmic heating in a food application : quality evaluation of cloudberry jam (2006)

Lindbom, Ingela, Kerry, J., Nunes, Helena Trindade, Pereira, Ricardo, Gallagher, Maria Jose Sousa, Staruch, Ladislav, ...

New techniques for food processing are areas of interest for the food industry and ohmic heating is an example of a new technique where there are many possible applications in the food area. The aim...

Application of a novel oscillatory flow micro-bioreactor to the production of γ-decalactone in a two immiscible liquid phase medium (2006)

Reis, N., Gonçalves, C. N., Aguedo, Mário, Gomes, Nelma, Teixeira, J. A., Vicente, A. A.

A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of γ-decalactone. A decrease up to 50% of the time required to...

Biosurfactants: potential applications in medicine (2006)

Rodrigues, L. R., Banat, Ibrahim M., Teixeira, J. A.

The use and potential commercial application of biosurfactants in the medical field has increased during the past decade. Their antibacterial, antifungal and antiviral activities make them relevant...

Kinetic study of fermentative biosurfactant production by Lactobacillus strains (2006)

Rodrigues, L. R., Moldes, Ana, Teixeira, J. A.

Screening of biosurfactant-producing ability of four Lactobacillus strains was performed, being shown that for all the tested strains biosurfactant production occurred mainly in the first 4 h. The...

Interference in adhesion of bacteria and yeasts isolated from explanted voice prostheses to silicone rubber by rhamnolipid biosurfactants (2006)

Rodrigues, L. R., Banat, Ibrahim M., Mei, Henny Van Der, Teixeira, J. A.

Aims: The effects and extent of adhesion of four different bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed rhamnolipid...

Trypsin hydrolysis of whey protein concentrates : characterization using multivariate data analysis (2006)

Mota, M. V. T., Oliveira, M. B. P., Rocha, Cristina, Teixeira, J. A., Torres, D., ...

Hydrolysis of whey protein concentrates (WPCs) was performed using trypsin under different combinations of temperatures and pH values in a total of three experiments, namely experiment A at 37 ºC...

Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria (2006)

Rodrigues, L. R., Teixeira, J. A., Mei, Henny Van Der

Optimization of the medium for biosurfactants production by probiotic bacteria (Lactococcus lactis 53 and Streptococcus thermophilus A) was carried out using response surface methodology. Both...

Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53 (2006)

Rodrigues, L. R., Teixeira, J. A., Mei, Henny Van Der

Isolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which...

Effects of ohmic heating technology in chemical properties of foods (2006)

Pereira, Ricardo, Pereira, Marta Duarte, Teixeira, J. A., Vicente, A. A.

In this work some food products where characterised chemically before and after ohmic and conventional heating treatments, comparing both processes. In each food type (cloudberry jam, goat milk) pH...

Death kinetics of Escherichia coli in goat milk and Bacillus licheniformis in cloudberry jam treated by ohmic heating (2006)

Pereira, Ricardo, Martins, Joana, Mateus, C., Teixeira, J. A., Vicente, A. A.

In recent years, the world’s food industry has focused increasing attention on electrical techniques of food processing. Ohmic heating is one of these techniques that can be considered as a high...

Kinetic model evaluation of ethanol fermentation from lactose (2006)

Juraščík, Martin, Klein, Jaroslav, Teixeira, J. A., Markoš, J.

This work presents a multi-route, nonstructural kinetic model for characterization of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both...

Design of a lab-on-a-chip for clinical tests of human physiological fluids (2006)

Miranda, J. M., Minas, Graça, Vicente, A. A., Teixeira, J. A.

Labs-on-a-chip are useful to perform in situ clinical tests with instantaneous results. In this work, the design phase of the development of a lab-on-a-chip is presented. The device will be used to...

Adaptive evolution of a recombinant lactose-consuming Saccharomyces cerevisiae strain (2006)

Guimarães, P., Parrou, J. L., Le Berre, V., François, J., Teixeira, J. A., Domingues, Lucília

In previous work, a recombinant S. cerevisiae flocculent strain (NCYC869-A3/T1, or simply T1) with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (lactose permease)...

New and simple plate test for screening relative transfructosylation activity of fungi (2006)

Dominguez, Ana, Santos, Isabel M., Teixeira, J. A., Lima, Nelson

Several microorganisms are reported to have transfructosylation activity due to fructosyltransferase and/or fructofuranosidase activities. However, the search for other fungi with higher...

Laccase activity from the fungus trametes hirsuta using an air-lift bioreactor (2006)

Rodríguez Couto, S., Rodríguez, A., Paterson, R. R. M., Lima, Nelson, Teixeira, J. A.

Aim: To produce high laccase activities from the white-rot fungus Trametes hirsuta in an in-house air-lift bioreactor (ALB). Methods and Results: Trametes hirsuta was grown in a 6-l ALB. A fed-batch...

Effect of solids on homogeneous–heterogeneous flowregime transition in bubble columns (2005)

Mena, P. C., Ruzicka, M. C., Rocha, F. A., Teixeira, J. A., Drahoš, J.

Experiments were conducted to study the effect of the presence of the solid phase on the homogeneous–heterogeneous flow regime transition in a bubble column 0.14m diameter. Air, distilled water and...

Simulation of liquid phase accumulation at centrifugal dewatering of activated sludge (2005)

Mota, M., Teixeira, J. A., Abaev, G. N., Yelshyna, I., Yelshin, Alexander

Centrifugation of activated sludge is a frequently used dewatering process but there is a need for a fitting function for moderate and high sludge concentrations. In particular, liquid phase...

Decalactone production by Yarrowia lipolytica under increased O2 transfer rates (2005)

Aguedo, Mário, Gomes, Nelma, Escamilla Garcia, E., Waché, Yves, Mota, M., Teixeira, J. A., ...

Yarrowia lipolytica converts methyl ricinoleate to γ-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-γ-decalactone produced by the yeast (263 mg lˉ¹) occurred by...

Using image analysis in the study of multiphase gas absorption (2005)

Mena, P. C., Pons, M. N., Teixeira, J. A., Rocha, F. A.

For the air–water–calcium alginate beads system, the effect of the presence of solids on the mass transfer characteristics in a bubble column was experimentally studied. Volumetric liquid side...

Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512(f) (2005)

Santos, Mariana, Rodrigues, Alírio, Teixeira, J. A.

The production of dextran and fructose from carob pod extract (CPE) and cheese whey (CW) as carbon source by the bacterium Leuconostoc mesenteroides was investigated. The influence of secondary...

Effect of solids on gas-liquid mass transfer and bubble characteristics in three-phase systems (2005)

Mena, P. C., Pons, M. N., Teixeira, J. A., Rocha, F. A.

Comunicação apresentada no "7th World Congress of Chemical Engineering" em Glasgow, Escócia, em Julho de 2005.

Yeast activity monitoring by fluorescent methods (2005)

Novak, J., Basarova, G., Fiala, J., Teixeira, J. A., Vicente, A. A.

Yeast activity was monitored during pilot plant yeast propagation under aerobic and anaerobic conditions. The work was carried out with three widely used Czech lager yeast strains. Fluorescent...

The challenging modelling of kLa in a periodic constricted small-scale tube (2005)

Reis, N., Vicente, A. A., Teixeira, J. A.

An extensive use in chemistry, biological and pharmaceutical laboratories is envisaged for a novel continuous screening reactor based on the oscillatory flow technology (Harvey, 2001). The basic unit...

Whey protein gelation : the effect of tryptic hydrolysis (2005)

Torres, D., Vicente, C. R., Teixeira, J. A., Gonçalves, M. P.

The ability of whey proteins (WP) to form heat induced gels and provide texture is desirable for some “solid like” foods like processed meat, dairy and bakery products. However, this property...

Retarding strawberry fruit senesce with edible coatings : case study (2005)

Ribeiro, Clara, Vicente, A. A., Teixeira, J. A., Miranda, Cândida

The objectives of this work were to study the ability of starch, carrageenan and chitosan based coatings to extend the shelf life of strawberry fruit (Fragaria ananassa). To do so, the surface...

Hidrólise de concentrado proteico de soro de queijo com tripsina imobilizada em resíduos da indústria cervejeira (2005)

Rocha, Cristina, Teixeira, J. A., Torres, D., Gonçalves, M. P., Mota, M. V. T., ...

Neste trabalho utilizou-se um sub-produto da indústria cervejeira proveniente do malte com um teor elevado em celulose, a drêche, como suporte de imobilização para tripsina. Efectuaram-se...

Use of two different carriers in a packed bed reactor for endopolygalacturonase production by a yeast strain (2005)

Almeida, Catarina, Brányik, Tomáš, Ferreira, Pedro Moradas, Teixeira, J. A.

A packed bed reactor (PBR) design was tested for the purpose of continuous pectinase production with yeasts, as a possible alternative to the traditional batch process using fungal cultures. Two...

Fluid mechanics and design aspects of a novel oscillatory flow screening mesoreactor (2005)

Reis, N., Harvey, Adam, Mackley, M. R., Vicente, A. A., Teixeira, J. A.

A novel, continuous, oscillatory flow screening mesoreactor, composed of tubes with smooth periodic constrictions, is presented as a new option for small-scale reaction engineering applications,...

Continuous beer fermentation using immobilized yeast cell bioreactor systems (2005)

Brányik, Tomáš, Vicente, A. A., Dostálek, Pavel, Teixeira, J. A.

Traditional beer fermentation and maturation processes use open fermentation and lager tanks. Although these vessels had previously been considered indispensable, during the past decades they were in...

Surface properties of Yarrowia lipolytica and their relevance to γ-decalactone formation from methyl ricinoleate (2005)

Aguedo, Mário, Waché, Yves, Belin, Jean-Marc, Teixeira, J. A.

The surface of the lipid-degrading yeast, Yarrowia lipolytica, was characterized by contact angle and zeta potential (ζ) measurements. The cells were mainly hydrophilic with a negative charge that...

Aspergillus niger β-galactosidase production by yeast in a continuous high cell density reactor (2005)

Domingues, Lucília, Lima, Nelson, Teixeira, J. A.

The continuous production of extracellular heterologous beta-galactosidase by a recombinant flocculating Saccharomyces cerevisiae, expressing the lacA gene (coding for beta-galactosidase) of...

Continuous primary fermentation of beer : yeast immobilization kinetics and product quality (2005)

Brányik, Tomáš, Vicente, A. A., Teixeira, J. A.

A one stage continuous primary beer fermentation consisting of brewing yeast immobilized on spent grain particles in a gas lift reactor was studied. The goal of this work was to adjust the flavor of...

Fermentation performance of a recombinant lactose-consuming flocculating Saccharomyces cerevisiae strain (2005)

Guimarães, P., Klein, Jaroslav, Domingues, Lucília, Teixeira, J. A.

In recent years, there has been a growing interest in bioreactors utilizing immobilized or flocculating cells in continuous process in order to improve the bioprocess productivity. One of possible...

Relationships between hydrodynamics and rheology of flocculating yeast suspensions in a high-cell-density airlift bioreactor (2005)

Klein, Jaroslav, Maia, J. M., Vicente, A. A., Domingues, Lucília, Teixeira, J. A., Juraščík, Martin

In this article a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high-celldensity system is presented. A highly flocculating recombinant strain of Sacharomyces...

Study of ternary glass spherical particle beds : porosity, tortuosity, and permeability (2005)

Dias, Ricardo P., Mota, M., Teixeira, J. A., Yelshin, Alexander

Ternary mixtures of glass beads were constructed as a model of granular packing. Porosity and permeability were experimentally determined in a wide range of mixture composition. Based on experimental...

Modeling of biosurfactant production by Lactobacillus Strains (2005)

Rodrigues, L. R., Moldes, Ana, Teixeira, J. A.

Screening of biosurfactant-producing ability of three Lactobacillus strains was performed, being shown that, for all the tested strains, biosurfactant production is occurring mainly in the first 7...

The effect of the electric field on lag-phase, ethanol and β-galactosidase production of a recombinant S. cerevisiae growing on lactose (2005)

Castro, I., Teixeira, J. A., Vicente, A. A.

The production of ethanol and β-galactosidase from cheese whey and other sub-products from industries can be a good way of minimizing environmental problems and producing valuable products from...

Spent-grains and zeolites as potential carriers for trypsin immobilisation (2005)

Rocha, Cristina, Ducso, L., Gonçalves, M. P., Teixeira, J. A.

Trypsin is a widely used enzyme for protein hydrolysis and can be used to improve functional and nutritional properties of foods. The immobilization of enzymes on solid supports can offer several...

Oxygen influence on the ß-oxidative metabolism of methyl ricinoleate for lactones production by the yeast Yarrowia lipolytica (2005)

Aguedo, Mário, Gomes, Nelma, Waché, Yves, Teixeira, J. A., Belo, Isabel

The yeast Yarrowia lipolytica is able to carry out the peroxisomal β-oxidative catabolism of methyl ricinoleate, a process leading notably to the production of γ-decalactone, a high-value aroma...

Impact of the surface properties of lactic bacteria on the stability of emulsions (2005)

Mai Huong Ly, Waché, Yves, Bouchez-Naïtali, M., Meylheuc, T., Aguedo, Mário, Teixeira, J. A., ...

Bacteria have physicochemical surface properties which depend on the chemical composition of the cell surface. These characters proceed from several type of physicochemical interactions and are...

Application of binary packing for chromatographic separation (2005)

Mota, M., Teixeira, J. A., Yelshin, Alexander, Dias, Ricardo P., Cortez, Susana

Separation of dextran and polyethylene glycol of different molecular mass was performed using a binary packed column of glass beads (size ratio ~ 10) and a binary packed column formed by kieselguhr-G...

On-line monitoring of multi-phase flow in a novel oscillatory screening reactor using fibre optical probes (2005)

Reis, N., Teixeira, J. A., Vicente, A. A.

This paper demonstrates the effectiveness of using fibre optic micro-probes for the measurement of dispersion and mixing in continuous flow within a novel screening reactor operating under...

Baker’s yeast filtration through mixed beds of filtration aids and large glass beads (2005)

Mota, M., Teixeira, J. A., Yelshin, Alexander

Filtration of baker´s yeast through a mixed particulate bed of filter aid and glass beads was investigated. Glass beads form the large size particle fraction in the mixture, whereas the small size...

Sorption of Cr(III) from aqueous solutions by spent brewery grain (2005)

Ferraz, A. I., Tavares, M. T. (Maria Teresa), Teixeira, J. A.

Two types of spent brewery grains were tested for Cr(III) uptake from aqueous solutions: not treated spent grains (NTSG), obtained by abundant washing of spent grain obtained from a Portuguese...

Aging of immobilized brewing yeast in a continuous bubble-column reactor (2005)

Brányik, Tomáš, Lehnert, Radek, Vicente, A. A., Teixeira, J. A.

The aim of this work was to study the physiological changes of immobilized brewing yeast Saccharomyces cerevisiae in a continuous reactor. Continuous cultivation was carried out at three different...

Trypsin immobilisation on zeolites (2005)

Rocha, Cristina, Gonçalves, M. P., Teixeira, J. A.

This work compares different types of zeolites (NaA, NaX and NaY) as potential carriers for trypsin immobilisation. Silica was also used as a traditional carrier. Covalent attachment to the carriers...

Valorization of olive mill wastewater by the yeast Yarrowia lipolytica (2005)

Araujo, Carla, Aguedo, Mário, Gomes, Nelma, Teixeira, J. A., Belo, Isabel

The aim of this work was to study the ability of two different strains of the yeast Yarrowia lipolytica to grow on Olive Mill Wastewater (OMW) and their potential to produce high-value products such...

Continuous beer fermentation with yeast immobilized on alternative cheap carriers and sensorial evaluation of the final product (2005)

Brányik, Tomáš, Silva, Daniel Pereira Da, Vicente, A. A., Teixeira, J. A.

Continuous beer fermentation offers a wide range of advantages, mostly of economic nature over the traditional batch process. However, due to increased complexity of operation comparing to batch...

Sensorial evaluation of continuously fermented beer and the role of process parameters in adjusting its flavour profile (2005)

Brányik, Tomáš, Silva, Daniel Pereira Da, Vicente, A. A., Ferreira, António Augusto, Dostálek, Pavel, ...

Although traditional batch overwhelmingly prevails over continuous fermentation, a breakthrough in quality and savings could be achieved by a simple, flexible and cheap fermentation system. This work...

Continuous primary fermentation of beer : yeast immobilization kinetics and product quality (2005)

Brányik, Tomáš, Vicente, A. A., Teixeira, J. A.

A one stage continuous primary beer fermentation consisting of brewing yeast immobilized on spent grain particles in a gas lift reactor was studied. The goal of this work was to adjust the flavor of...

Novel and simple test plating for screening relative transfructosylation activity of fungi (2005)

Dominguez, Ana, Santos, Isabel M., Teixeira, J. A., Lima, Nelson

Fructooligosaccharides (FOS) have received particular attention recently because of their excellent biological and functional properties, namely, as prebiotic compounds that promote the growth of...

Effect of the particle shape on flow through porous media (2005)

Mota, M., Teixeira, J. A., Yelshin, Alexander, Cortez, Susana

In order to study the performance of shaped particles flow in porous media, filtration of two different shape - spherical and rod-like – micro particles was performed through a porous bed....

Low-cost fermentative medium for biosurfactant production by Streptococcus thermophilus A (2005)

Rodrigues, L. R., Teixeira, J. A.

Interest in biosurfactants has increased considerably in recent years, as they are potential candidates for many commercial applications in the petroleum, pharmaceuticals, biomedical and food...

Liquid-liquid equilibrium of aqueous polymer two-phase systems using the modified wilson equation (2005)

Madeira, Pedro, Xin Xu, You-Ting Wu, Teixeira, J. A., Macedo, Eugénia

The modified Wilson equation, developed in a previous publication, for the excess Gibbs energy of aqueous polymer solutions, is extended to multicomponent mixtures. The model provides a flexible...

Prebiotics : from concept to product : the BIOLIFE project (2005)

Torres, D., Dominguez, Ana, Rodrigues, L. R., Rocha, I., Teixeira, J. A., Lima, Nelson, ...

Nutrition has progressed from the discovery of essential nutrients and prevention of dietary deficiency to the promotion of a state of well-being and health and the reduction of the risk of disease....

Biotechnological application of Yarrowia lipolytica for biotransformation in biphasic media (2005)

Gomes, Nelma, Aguedo, Mário, Teixeira, J. A., Belo, Isabel

The yeast Yarrowia lipolytica is able to grow on hydrophobic substrates such as oils, alkanes, fatty acids1. This characteristic notably offers interesting prospects in different industrial areas:...

Aseptic processing of a strawberry pulp in a continuous ohmic heater : numerical simulations and model validation (2005)

Castro, I., Reis, N., Teixeira, J. A., Vicente, A. A.

Continuous food-sterilization processes often involve the flow of liquids or solid-liquid mixtures, frequently highly viscous and with non-Newtonian behaviour, in pipes and reactors. Heat treatment...

Physicochemical surface properties of brewing yeast influencing their immobilization onto spent grains in a continuous reactor (2004)

Brányik, Tomáš, Vicente, A. A., Teixeira, J. A.

Immobilization of brewing yeast onto a cellulosebased carrier obtained from spent grains, a brewing byproduct, by acid/base treatment has been studied in a continuously operating bubble-column...

Study of hydrodynamics and mixing in an airlift reactor with an enlarged separator using magnetic tracer method (2004)

Klein, Jaroslav, Vicente, A. A., Teixeira, J. A.

The magnetic tracer technique was used to obtain essential information on hydrodynamics, mixing and reactor design in a multiphase airlift reactor with an enlarged separator. The tracer method...

The use of fibre optic probes for flow monitoring within a small-scale tube (2004)

Reis, N., Vicente, A. A., Teixeira, J. A., Mackley, M. R.

This paper demonstrates the effectiveness of using fibre optic micro-probes for the measurement of dispersion and mixing in continuous flow within small-scale tubes under oscillatory flow conditions....

CFD simulations of RTD of a strawberry pulp in a continuous ohmic heater (2004)

Castro, I., Reis, N., Teixeira, J. A., Vicente, A. A.

A pilot ohmic heater is to be tested for the continuous aseptic processing of strawberry pulps and jams. The hydrodynamics and the fluid residence time distribution (RTD) have been experimentally...

Application of high cell density airlift bioreactors to bio-ethanol production – study on optimal bioreactor operation (2004)

Klein, Jaroslav, Vicente, A. A., Maia, J. M., Domingues, Lucília, Teixeira, J. A.

In this paper a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high cell density system is presented. A highly flocculating recombinant strain of Sacharomyces cerevisae...

Influence of biosurfactants from probiotic bacteria on formation of biofilms on voice prostheses (2004)

Rodrigues, L. R., Mei, Henny Van Der, Teixeira, J. A.

Biofilms were grown on preconditioned voice prostheses with biosurfactants obtained from probiotic bacteria Lactococcus lactis 53 and Streptococcus thermophilus A in an artificial throat model. Both...

Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicone rubber (2004)

Rodrigues, L. R., Mei, Henny Van Der, Teixeira, J. A.

The ability of biosurfactant obtained from the probiotic bacterium Lactococcus lactis 53 to inhibit adhesion of four bacterial and two yeast strains isolated from explanted voice prostheses to...

The use of enzymes and microorganisms for the production of aroma compounds from lipids (2004)

Aguedo, Mário, Mai Huong Ly, Belo, Isabel, Teixeira, J. A., Belin, Jean-Marc, Waché, Yves

Lipids are an important source of aroma compounds. In foods, lipids are degraded or modified by enzymatic activities that are originally present in the raw materials or that develop later due to the...

Preparation of controlled particulate mixtures with glass beads of different sizes (2004)

Dias, Ricardo P., Teixeira, J. A., Mota, M., Yelshin, Alexander

A method of mixing/preparing binary and ternary mixtures of glass beads was developed using a viscous solution of glycerol in water. After mixing, the mixture was transferred to a prismatic vessel...

Particulate binary mixtures : dependence of packing porosity on particle size ratio (2004)

Dias, Ricardo P., Teixeira, J. A., Mota, M., Yelshin, Alexander

Binary mixtures with significant size ratios are scarcely studied. Yet, contaminants of chromatographic columns or ion-exchange resins have size ratios of delta < 0.1. Binary mixtures of glass beads...

Changes in diffusion through the brain extracellular space (2004)

Mota, M., Teixeira, J. A., Keating, José B., Yelshin, Alexander

ECS (extracellular space) works as the microenvironment of brain cells. Diffusion through ECS may be described through an effective diffusion coefficient, De, which in turn depends on ECS porosity,...

Growth model and metabolic activity of brewing yeast biofilm on the surface of spent grains : a biocatalyst for continuous beer fermentation (2004)

Brányik, Tomáš, Vicente, A. A., Kuncová, Gabriela, Podrazký, Ondřej, Dostálek, Pavel, Teixeira, J. A.

In the continuous systems, such as continuous beer fermentation, immobilized cells are kept inside the bioreactor for long periods of time. Thus an important factor in the design and performance of...

The effect of electric field on important food-processing enzymes : comparison of inactivation kinetics under conventional and ohmic heating (2004)

Castro, I., Macedo, B., Teixeira, J. A., Vicente, A. A.

This work deals with the determination of the inactivation kinetics of several enzymes, most of them used as time-temperature integrators in the food industry. The tested enzymes were...

Ohmic heating of strawberry products : electrical conductivity measurements and ascorbic acid degradation kinetics (2004)

Castro, I., Teixeira, J. A., Salengke, S., Sastry, S. K., Vicente, A. A.

The effects of field strength and multiple thermal treatments on electrical conductivity of strawberry products were investigated. Electrical conductivity increased with temperature for all the...

Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose (2004)

Domingues, Lucília, Castro, I., Lima, Nelson, Teixeira, J. A.

Improved productivity and costs reduction in fermentation processes may be attained by using flocculating cell cultures. The production of extracellular heterologous β-galactosidase by recombinant...

Cr(III) removal and recovery from Saccharomyces cerevisiae (2004)

Ferraz, A. I., Tavares, M. T. (Maria Teresa), Teixeira, J. A.

Heavy metal recovery from biosorbents is of major importance in the assessment of competitiveness of biosorption processes. Several desorption agents (H2SO4, HNO3, HCl, CH3COOH and EDTA) were tested...

Residence times and mixing of a novel continuous oscillatory flow screening reactor (2004)

Reis, N., Vicente, A. A., Teixeira, J. A., Mackley, M. R.

This paper is concernedwith the fluidmechanics andmixing performance of a novel oscillatory flow screening reactor. Using fibre optic probes, a mixing coefficient k_m is determinedfor the system as a...

Continuous primary fermentation of beer with yeast immobilized on spent grains : the effect of operational conditions (2004)

Brányik, Tomáš, Vicente, A. A., Cruz, José Machado, Teixeira, J. A.

A one-stage continuous primary beer fermentation with immobilized brewing yeast was studied. The objective of the work was to optimize the operational conditions (aeration and temperature) in terms...

Dependence of Saccharomyces cerevisiae filtration through membrance on yeast concentration (2004)

Mota, M., Teixeira, J. A., Yelshin, Alexander

Filtration of baker’s yeast in an isotonic solution through a 0.45 micron membrane was investigated for yeast concentrations in the range 0.14 – 51 g/L at filtration pressures between 40 and 80...

Effect of real particles packing with large size ratio on porosity and tortuosity of filter bed (2004)

Mota, M., Teixeira, J. A., Dias, Ricardo P., Yelshin, Alexander

The complexity of processes involved in the formation of granular beds results in limited information about permeability κ , which directly rela tes with packing porosity ε and tortuosity T . For a...

Enzymatic hydrolysis of whey protein concentrates : peptide HPLC profiles (2004)

Mota, M. V. T., Oliveira, M. B. P., Rocha, Cristina, Teixeira, J. A., Torres, D., ...

Hydrolysis of whey protein concentrates (WPCs) at different temperatures and pHs, using three enzymes: pepsin, trypsin, and Alcalase®, was monitored during more than 5 hr by reversed phase HPLC/UV,...

Biosurfactant from Streptococcus thermophilus A inhibit microbial adhesion on silicone rubber (2004)

Rodrigues, L. R., Mei, Henny Van Der, Teixeira, J. A.

The ability of the biosurfactant obtained from Streptococcus thermophilus A to inhibit adhesion of four bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber...

Simulações numéricas do processamento asséptico de polpas de morango num aquecedor óhmico contínuo (2004)

Castro, I., Reis, N., Teixeira, J. A., Vicente, A. A.

Neste trabalho efectuaram-se simulações numéricas dos padrões de fluxo num aquecedor óhmico contínuo, em estado estacionário, com recurso ao programa Fluent™. Para tal, criou-se uma grelha...

Residence times and mixing of a novel continuous oscillatory flow meso reactor (2004)

Reis, N., Vicente, A. A., Teixeira, J. A., Mackley, M. R.

A novel meso reactor based on oscillatory flow technology (Harvey et al., 2001) has been recently presented in Harvey et al. (2003) as a new technology for reaction engineering and particle...

Enhanced mass transfer rates of a novel oscillatory flow screening reactor (2004)

Reis, N., Vicente, A. A., Teixeira, J. A., Mackley, M. R.

A novel continuous small-scale reactor based on the oscillatory flow technology (Harvey et al 2001) is being developed for application to specialist chemical manufacture and high throughput...

Hydrodynamic analysis of a continuous airlift bioreactor with flocculating high cell density system (2004)

Klein, Jaroslav, Vicente, A. A., Domingues, Lucília, Juraščík, Martin, Teixeira, J. A.

One of the most common ways to improve the productivity of a fermentation process is the use of high cell density systems. In practice, such a system usually represents a threephase...

Effect of solids on flow regime transition in three-phase bubble columns (2004)

Mena, P. C., Rocha, F. A., Teixeira, J. A., Drahoš, J., Ruzicka, M. C.

In bubble column reactors there are two principal flow regimes [1,2,3], the homogeneous (HoR) and the heterogeneous (HeR). These reactors have different behaviour in HoR and HeR, thus the dependences...

Numerical simulations of the hydrodynamic behaviour of a continuous ohmic heater operating with Newtonian and Non-Newtonian fluids (2004)

Castro, I., Reis, N., Teixeira, J. A., Vicente, A. A.

A pilot ohmic heater was tested for the continuous aseptic processing of strawberry pulp. The fluid mechanics and the fluid residence time distribution (RTD), in the whole volume of the vessel and...

Hydrodynamics of a three-phase airlift reactor with an enlarged separator : application to high cell density systems (2003)

Klein, Jaroslav, Vicente, A. A., Teixeira, J. A.

The hydrodynamic study of a three-phase airlift reactor with an enlarged head zone was carried out. Effects of gas-solid-liquid separator design and riser to downcomer cross-sectional area using...

The influence of field strength, sugar and solid content on electric conductivity of strawberry products (2003)

Castro, I., Teixeira, J. A., Salengke, S., Sastry, S. K., Vicente, A. A.

The effects of field strength, soluble solids (from 14 to 59.5 ºBrix) and particle size (using two size distributions) on electrical conductivity were investigated. Electrical conductivity increased...

Recovery of the proteose peptone component 3 from cheese whey in Reppal PES 100/polyethylene glycol aqueous two-phase systems (2003)

Rodrigues, L. R., Venâncio, Armando, Teixeira, J. A.

Recovery of the proteose peptone component 3 from cheese whey was optimal using a 16% (w/w) Reppal PES 100 – 24% (w/w) PEG 600 aqueous two-phase system, at pH 7, giving a mass recovery yield of 99%...

Hydrodynamic considerations on optimal design of a three-phase airlift bioreactor with high solids loading (2003)

Klein, Jaroslav, Vicente, A. A., Teixeira, J. A.

The hydrodynamic study of a three-phase airlift (TPAL) bioreactor with an enlarged gas–liquid dual separator was carried out. Different lengths and diameters of the draft tube were tested to show...

The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei (2003)

Lemos, M. A., Teixeira, J. A., Domingues, M. R. M., Mota, M., Gama, F. M.

The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained...

Interference of coarse and fine particles of different shape in mixed porous beds and filter cakes (2003)

Mota, M., Teixeira, J. A., Bowen, William R., Yelshin, Alexander

In solid–liquid separation the knowledge of solids packing structure is important to control permeability and dewaterability. In particular, cakes formed in filtration are often represented by the...

Continuous production of pectinase by immobilized yeast cells on spent grains (2003)

Almeida, Catarina, Brányik, Tomáš, Ferreira, Pedro Moradas, Teixeira, J. A.

A yeast strain secreting endopolygalacturonase was used in this work to study the possibility of continuous production of this enzyme. It is a feasible and interesting alternative to fungal batch...

Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3-phosphate dehydrogenase (2003)

Almeida, Catarina, Queirós, Odília, Wheals, Alan, Teixeira, J. A., Ferreira, Pedro Moradas

The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces...

Aqueous two-phase extraction using thermoseparating polymer : a new system for the separation of endo-polygalacturonase (2003)

Pereira, Martinha, You-Ting Wu, Venâncio, Armando, Teixeira, J. A.

Aqueous two-phase partitioning of endo-polygalacturonase (endo-PG) produced by Kluyveromyces marxianus strains was studied, on systems containing the thermoseparating polymer Ucon 50-HB-5100 (a...

Effect of viscosity on homogeneous–heterogeneous flow regime transition in bubble columns (2003)

Ruzicka, M. C., Drahoš, J., Mena, P. C., Teixeira, J. A.

Experiments were performed in a cylindrical 0.14m diameter bubble column with a metal perforated plate. Air and aqueous solutions of glycerol with viscosity 1–22mPa s were the phases. Gas holdup...

A new modified Wilson equation for the calculation of vapor–liquid equilibrium of aqueous polymer solutions (2003)

Xin Xu, Madeira, Pedro, Teixeira, J. A., Macedo, Eugénia

A local composition model based on the lattice theory and two-fluid theory, considering the excess heat capacity, which is a modified Wilson equation, is developed for the excess Gibbs energy of...

An integrated approach for cheese whey lactose valorisation (2003)

Domingues, Lucília, Lima, Nelson, Teixeira, J. A.

The development of any fermentation process requires the integration of life and engineering sciences with the aim of obtaining a high productivity system. The use of immobilized cells is, without...

Residence time of tagged magnetic particle in an internal-loop airlift reactor with an enlarged dual separator (2003)

Klein, Jaroslav, Vicente, A. A., Teixeira, J. A.

European Community (EC) - Improving Human Research Potential - Marie Curie Fellowship - contract number MCFI-2001-01504.

Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall : effect on cell surface properties and immobilization (2002)

Nakari-Setälä, T., Azeredo, Joana, Henriques, Mariana, Teixeira, J. A., Linder, M., ...

The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell...

O ensino de engenharia biológica na Universidade do Minho (2002)

Ferreira, E. C., Teixeira, J. A.

Nos anos 80, a Biotecnologia foi considerada, a nível mundial, como uma das áreas prioritárias de desenvolvimento. Isto levou a que algumas universidades portuguesas começassem a oferecer alguma...

Continuous primary beer fermentation with brewing yeast immobilized on spent grains (2002)

Brányik, Tomáš, Vicente, A. A., Cruz, José Machado, Teixeira, J. A.

This work demonstrated the technological feasibility of the three-phase airlift bioreactor (ALR) with brewing yeast immobilized on spent grains (a brewing by-product) for continuous beer production....

Comparative study of Selva and Camarosa strawberries for the commercial market (2002)

Castro, I., Gonçalves, O., Teixeira, J. A., Vicente, A. A.

Selva and Camarosa strawberry varieties were characterized chemically and physically. The importance of keeping the stem until processing, the influence of different transport periods under...

Influence of cell-shape on the cake resistance in dead-end and cross-flow filtrations (2002)

Mota, M., Teixeira, J. A., Yelshin, Alexander

The problems related to tortuosity variation whenever filter cakes are composed of cells with different shapes and compressible biosuspensions are discussed. Presented examples show that neglecting...

Dextran and fructose separation on an SMB continuous chromatographic unit (2002)

Coelho, Mariana S., Azevedo, Diana C. S., Teixeira, J. A., Rodrigues, Alírio

A dextran/fructose mixture was obtained by fermentation of a sucrose rich media using Leuconostoc mesenteroides NRRL B512(f). The dextran and fructose mixture obtained by fermentation as well as a...

Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger β-galactosidase (2002)

Domingues, Lucília, Teixeira, J. A., Penttilä, M., Lima, Nelson

A flocculent Saccharomyces cerevisiae strain secreting Aspergillus niger β-galactosidase activity was constructed by transforming S. cerevisiae NCYC869-A3 strain with plasmid pVK1.1 harboring the A....

Immobilized particles in gel matrix-type porous media : nonhomogeneous cell distribution (2002)

Mota, M., Teixeira, J. A., Yelshin, Alexander

The conventional random pore model assumes a homogeneous cell distribution in the gel matrix used to immobilize cells. However, the validity of this model is restricted to values of the exponent R,...

Role of fractional cake composition in cake resistance (2002)

Yelshin, Alexander, Teixeira, J. A., Bowen, William R., Mota, M.

The binary mixture model was applied in order to provide an explanation of the discrepancy between the calculated and experimentally measured specific cake resistance in TiO2 cakes. The case under...

Flocculation bioreactors : engineering based problems (2001)

Vicente, A. A., Teixeira, J. A.

The use of flocculation bioreactors is one of the most interesting ways to provide an increase of the productivity of biotechnological processes. As other high cell density systems, flocculation...

Alcohol production from cheese whey permeate using genetically modified flocculent yeast cells (2001)

Domingues, Lucília, Lima, Nelson, Teixeira, J. A.

Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for β-galactosidase) and LAC12 (coding for...

Effect of airflow rate on yields of Steinernema carpocapse Az 20 in liquid culture in an external-loop airlift bioreactor (2001)

Neves, J. M., Teixeira, J. A., Simões, N., Mota, M.

Maximization of the contact between males and females is a key factor in the production of the nematode Steinernema carpocapsae in a bioreactor. The influence of the airflow rate in male and female...

Partitioning and separation of α-lactalbumin and β-lactoglobulin in polyethylene glycol/ammonium sulphate aqueous two-phase systems (2001)

Rodrigues, L. R., Venâncio, Armando, Teixeira, J. A.

The partition behaviour of α-lactalbumin (αla) and β-lactoglobulin (βlg) on PEG/(NH4)2SO4 system was studied. For purified proteins, a partition coefficient of 12.8 for αla and 0.34 for βlg,...

Spent grains : a new support for brewing yeast immobilisation (2001)

Brányik, Tomáš, Vicente, A. A., Cruz, José Machado, Teixeira, J. A.

A novel carrier obtained from spent grains, a brewing by-product, was used for brewing yeast immobilisation in a continuous bubble-column reactor. The multiple-layer cell adhesion to the carrier...

Oxygen mass transfer in a high solids loading three-phase internal-loop airlift reactor (2001)

Teixeira, J. A.

Determinations of volumetric mass transfer coefficient were conducted in a three-phase internal-loop airlift reactor with an enlarged degassing zone. The effect of parameters such as the airflow rate...

Immobilized particles in gel matrix-type porous media : homogeneous porous media model (2001)

Mota, M., Teixeira, J. A., Yelshin, Alexander

Diffusion in pure gels and gels with immobilized cells was analyzed. A model of diffusion assuming a homogeneous cell distribution in gel was improved by introducing a tortuosity value. By...

Binary spherical particle mixed beds : porosity and permeability relationship measurement (2001)

Mota, M., Teixeira, J. A., Bowen, William R., Yelshin, Alexander

A continuous function relating the porosity of a mixture, ε, and the large particle volume fraction, xD, of binary mixtures of spherical particles has been established. The incorporation of the...

Separation of endo-polygalacturonase using aqueous two-phase partitioning (2001)

You-Ting Wu, Pereira, Martinha, Venâncio, Armando, Teixeira, J. A.

The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)–polyvinyl alcohol (PVA10 000) and PEG–hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied,...

Immobilised particles in gels : homogeneous porous media model (2001)

Mota, M., Teixeira, J. A., Yelshin, Alexander

A model of diffusion assuming a homogeneous cell distribution in a gel was improved by considering the effect of tortuosity. By theoretical analysis and numerical modeling it was shown that the...

Oxygen mass transfer in a high solids loading three-phase internal-loop airlift reactor (2001)

Teixeira, J. A.

The way volumetric mass transfer coefficient in a 60 L three-phase internal-loop airlift reactor with an enlarged degassing zone is influenced by the airflowrate (riser superficial gas velocities...

Modelling design of multiphase bubble-bed reactors for advanced food-industry applications (2001)

Zahradnik, Jindrich, Ruzicka, M. C., Markoš, J., Teixeira, J. A., Generalis, S., Thomas, N.

An EC project (IC15-CT98-0904 / PL979021) under this title commenced November 1998 courtesy of Dr Jindrich Zahradnik, sadly since deceased. In dedication to his memory overviewed here are...

Characterisation of new aqueous two-phase systems using thermoseparating polymers (2001)

Pereira, Martinha, Madeira, Pedro, Wu, You-Ting, Teixeira, J. A., Macedo, Eugénia

Aqueous two-phase systems (ATPS) are increasingly being used for separation of proteins, cells and viruses and some small molecular weight substances since they offer a gentle and friendly...

Cr (III) recovery from Saccharomyces cerevisiae by elution : a preliminary study (2000)

Ferraz, A. I., Tavares, M. T. (Maria Teresa), Teixeira, J. A.

To recover adsorbed metals from biosorbents, in order to recycle metal and reuse biomass in several adsorption/desorption cycles, elution conditions need to be optimized. The present work aimed to...

Recovery of endo-polygalacturonase using polyethylene glycol-salt aqueous two-phase extraction with polymer recycling (2000)

Wu, You-Ting, Pereira, Martinha, Venâncio, Armando, Teixeira, J. A.

The partitioning behaviour of endo-polygalacturonase (endo-PG) and total protein from a clarified Kluyveromyces marxianus fermentation broth in polyethylene glycol (PEG)-ammonium sulfate and...

Contamination of a high-cell-density continuous bioreactor (2000)

Domingues, Lucília, Lima, Nelson, Teixeira, J. A.

Continuous fermentations were carried out with a recombinant flocculent Saccharomyces cerevisiae strain in an airlift bioreactor. Once operating under steady state at a dilution rate of 0.45 h−1,...

Incorporação de proteínas lácteas no queijo de vaca (2000)

Couto, A., Lopes, Zulmira, Venâncio, Armando, Teixeira, J. A.

O soro de leite, também conhecido como soro de queijo, é um efluente da produção de queijo gerado em grandes quantidades, estimando-se uma produção diária em Portugal de cerca de um milhão de...

Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase (2000)

Domingues, Lucília, Teixeira, J. A., Lima, Nelson, Penttilä, M.

One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in...

Applications of yeast flocculation in biotechnological processes (2000)

Domingues, Lucília, Vicente, A. A., Lima, Nelson, Teixeira, J. A.

A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects – the basics...

Hydrodynamics of a three-phase external-loop airlift bioreactor (2000)

Fialová, Maria, Zahradnik, Jindrich, Teixeira, J. A.

The effect of the distributing plate orifice diameter, airflow rate, solids loading and solids density on the hydrodynamic characteristics - gas holdup, circulation time and liquid velocity - of a...

A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease (2000)

Lemos, M. A., Teixeira, J. A., Mota, M., Gama, F. M.

Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated...

Production of dextransucrase, dextran and fructose from sucrose using Leuconostoc mesenteroides NRRL B512(f) (2000)

Santos, Mariana, Teixeira, J. A., Rodrigues, Alírio

The production of dextransucrase, dextran and fructose by sucrose fermentation using Leuconostoc mesenteroides NRRL-B512(F) was studied in batch operation in a bioreactor with total working volume of...

Effect of tortuosity on transport properties of mixed granular beds (2000)

Mota, M., Teixeira, J. A., Bowen, William R., Yelshin, Alexander

The properties of binary mixed beds were investigated in a wide range of the particle size ratio δ . The relationship between tortuosity T, porosity ε, δ , and volume fraction of the large...

Continuous ethanol fermentation of lactose by a recombinant flocculating saccharomyces cerevisiae strain (1999)

Domingues, Lucília, Dantas, Maria M., Lima, Nelson, Teixeira, J. A.

Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease)...

Image modeling of mixed granular porous media (1999)

Mota, M., Teixeira, J. A., Yelshin, Alexander

Two-dimensional image modeling of binary and ternary mixed porous media was used to study the effect of porosity and tortuosity on effective diffusion coefficient and permeability were separated....

Increase of ethanol productivity in an airlift reactor with a modified draught tube (1999)

Vicente, A. A., Dluhý, M., Teixeira, J. A.

Static mixers were introduced in the draught tube of a three-phase, concentric tube type airlift bioreactor, resulting in a 30% increase in ethanol productivity during glucose fermentation with a...

Hydrodynamics of a three-phase external-loop airlift bioreactor (1999)

Fialová, Maria, Zahradnik, Jindrich, Teixeira, J. A.

The effect the distributing plate orifice diameter, airflow rate, solids loading and solids density on the hydrodynamic characteristics – gas holdup, circulation time and liquid velocity – of a...

Construction of a flocculent saccharomyces cerevisiae fermenting lactose (1999)

Domingues, Lucília, Teixeira, J. A., Lima, Nelson

A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was...

The use of flocculating brewer's yeast for Cr(III) and Pb(II) removal from residual wastewaters (1999)

Ferraz, A. I., Teixeira, J. A.

The use of inexpensive biosorbents to sequester heavy metals from aqueous solutions, is one of the most promising technologies being developed to remove these toxic contaminants from wastewaters....

Hydrodynamic model for three-phase internal- and external-loop airlift reactors (1999)

Fialová, Maria, Zahradnik, Jindrich, Teixeira, J. A.

A mathematical model predicting the hydrodynamic behaviour of three-phase airlift reactors, working with low-density solids and with high solids loading, was developed. The model allows for the...

Removal efficiency of Cu2+, Cd2+ and Pb2+ by waste brewery biomass : pH and cation association effects (1999)

Marques, Paula Alexandra, Pinheiro, Helena Maria, Teixeira, J. A., Rosa, M. F.

In this work two distinct (flocculent and non-flocculent) yeast wastes from Portuguese breweries were used for the selective removal of Cu2+, Cd2+ and Pb2+ from aqueous solutions. One of the goals...

Image analysis of packed beds of spherical particles of different sizes (1999)

Mota, M., Teixeira, J. A., Yelshin, Alexander

Two-dimensional simulations of packed beds composed of binary and ternary particle mixtures were made and image analysis of the bed structure was used to determine the bed porosity and tortuosity....

Isolation, screening, and characterisation of flocculating and pectinase producing Kluyveromyces strains (1999)

Pereira, Martinha, Schwan, Rosane, Teixeira, J. A.

Eleven strains of Kluyveromyces marxianus were studied to select the best producers of extracellular endo-polygalacturonase (endo-PG), using glucose and lactose as carbon source. Flocculation ability...

Novas metodologias para a fermentação alcoólica do soro de queijo (1999)

Domingues, Lucília, Lima, Nelson, Teixeira, J. A.

Em Portugal, são diariamente lançados no meio ambiente cerca de 1 milhão de litros de soro de queijo – fracção líquida resultante do fabrico de queijo. O impacte ambiental ocasionado por tal...

On-line estimation of biomass through pH control analysis in aerobic yeast fermentation systems (1998)

Vicente, A. A., Castrillo, Juan I., Teixeira, J. A., Ugalde, Unai

The amount of acid or base consumed in yeast cultures has been recently assigned to the pathway of nitrogen assimilation under respiratory conditions with no contribution by carbon metabolism...

Solid-phase distribution in an airlift reactor with an enlarged degassing zone (1998)

Teixeira, J. A.

The distribution of the solid-phase in an airlift reactor of the concentric draught tube type, with an enlarged degassing zone, has been determined. Samples were taken at eight points of the reactor...

Mass transfer properties of glucose and O2 in Saccharomyces cerevisiae flocs (1998)

Vicente, A. A., Dluhý, M., Ferreira, E. C., Mota, M., Teixeira, J. A.

The use of flocculent microorganisms in fermentations is a very promising application. However, it has some drawbacks of which the most important ones are related to mass transfer limitations within...

Modelling diffusion-reaction phenomena in yeast flocs of Saccharomyces cerevisiae (1998)

Vicente, A. A., Dluhý, M., Ferreira, E. C., Teixeira, J. A.

Limitations in the diffusion of substrates into the flocs will condition cell metabolic behaviour, having obvious consequences on growth and product formation. Polymeric additives have been used...

Cutinase purification on poly(ethylene glycol)–hydroxypropyl starch aqueous two-phase systems (1998)

Almeida, M. C., Venâncio, Armando, Teixeira, J. A., Aires-Barros, M. R.

The partition behaviour of cutinase on poly(ethylene glycol) (PEG)–hydroxypropyl starch aqueous two-phase systems was characterized. The effect of molecular mass of PEG, the pH of the system and...

Hydrodynamic studies in an airlift reactor with an enlarged degassing zone (1998)

Teixeira, J. A.

The hydrodynamic behaviour of a 60 l threephase airlift bioreactor, of the concentric draught tube type, with an enlarged degassing zone has been studied. Ca-alginate beads were used as the solid...

Effect of liquid-phase surface tension on hydrodynamics of a three-phase airlift reactor with an enlarged degassing zone (1998)

Teixeira, J. A.

The effect of the addition of ethanol (10 g/l) to the liquid-phase on gas and solids holdup, circulation and mixing times and interstitial liquid velocity in a threephase airlift reactor was...

Yeast flocculation: a new method for characterising cell surface interactions (1997)

Azeredo, Joana, Ramos, Ilia, Rodrigues, L. R., Teixeira, J. A.

Flocculation is a property of yeast cells that has been traditionally used in industrial fermentations, especially in the brewing and wine industries. It is a cell wall interaction and an increase in...

A new sampling device for measuring solids hold-up in a three-phase system (1997)

Vicente, A. A., Mota, M., Teixeira, J. A.

Measurement of solids hold-up in gas-liquid-solid systems was achieved by using a cylinder with a valve in each extremity, which allowed direct sampling inside the reactor causing minimal...

Rapid and sensitive detection of ß-galactosidase-producing yeasts by using microtiter plater assay (1997)

Domingues, Lucília, Teixeira, J. A., Lima, Nelson

Yeast β-galactosidase activity was detected by a microtiter plate assay using pNPG or Xgal as substrate in 30 minutes. The detection gave a clear result which is well correlated with the specific...

Characterization of sugar diffusion coefficients in alginate membranes (1997)

Venâncio, Armando, Teixeira, J. A.

The diffusivity of several monosaccharides and disaccharides in calcium alginate gels was determined usig a specially designed diaphragm cell. The diffusion coefficients of the tested sugars are 4 to...

A new technique for measuring kinetic and mass transfer parameters in flocs of Saccharomyces cerevisiae (1997)

Vicente, A. A., Dluhý, M., Teixeira, J. A.

Yeast flocs are very fragile and cause problems when manipulations are needed. A new equipment for measuring kinetic and mass transfer parameters was developed and tested: the specific O2 uptake...

Trivalent chromium sorption on alginate beads (1997)

Araújo, M. Manuela, Teixeira, J. A.

The applicability of trivalent chromium removal from aqueous solutions using calcium alginate beads was studied. The equilibrium isotherms were plotted at two temperatures. The relationship between...

Porous media behaviours modelling and analysis in separation processes (1997)

Yelshin, Alexander, Mota, M., Teixeira, J. A.

The main parameters are used to define effective diffusion coefficient and permeability of the medium is porosity, ε, tortuosity, T, and particle diameter. The qualitative model of the porosity and...

Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers (1996)

Venâncio, Armando, Almeida, Catarina, Teixeira, J. A.

The main drawback when using aqueous two-phase systems for macromolecule purification is the high cost of most polymers used. The purification of an enzyme, alcohol dehydrogenase, from a crude...

Determinação das condições de arejamento necessárias à produção de nemátodos em biorreactor (1996)

Neves, J. M., Teixeira, J. A., Simões, N., Mota, M.

Determinou-se o consumo de oxigénio para o estado adulto e para os outros estados de desenvolvimento (L1- L4) de Steinernema carpocapsae estirpe Az 20. Verificou-se que o consumo de oxigénio pelo...

Remoção de crómio de efluentes industriais utilizando geis de alginato (1996)

Teixeira, J. A., Araújo, M. Manuela

A utilização de biopolímeros como materiais biossorventes na remoção de metais pesados de efluentes industriais é uma alternativa atraente em relação aos dispendiosos métodos...

Kinetics and stoichiometric of a batch fermentation using a highly flocculent strain of Saccharomyces cerevisiae for different aeration rates and initial sugar concentrations (1996)

Vicente, A. A., Ferreira, E. C., Teixeira, J. A.

Flocculating cultures are one of the most interesting technologies to immobilise cells inside bioreactors. However, little is known about cell metabolism, viability and fermentation kinetics and...

Cell wall surface properties and flocculence of a Kluyveromyces marxianus strain (1995)

Teixeira, J. A., Azeredo, Joana, Sousa, M., Sil, C.

Yeast flocculation is under genetic control and is described as a cell wall interaction. This characteristic of yeast cells has been traditionally used in industrial fermentation processes. The...

Protein partition on a derivative guar gum based aqueous two-phase system (1995)

Venâncio, Armando, Almeida, Catarina, Domingues, Lucília, Teixeira, J. A.

A new aqueous two-phase system based on a guar gum derivative, commercialized under the trade name of Solvitose Gum Ofa, and poly(ethylene glycol) is described. Its main difference from other...

Protein mass transfer studies on a spray column using the PEG-Reppal PES 100 aqueous two-phase system (1995)

Venâncio, Armando, Teixeira, J. A.

The characterization of Bovine Serum Albumin mass transfer mechanisms in a spray column using an aqueous two-phase system composed of poly(ethylene glycol) and a modified starch-Reppal PES 100-is...

Model identification and diffusion coefficients determination of glucose and malic acid in calcium alginate membranes (1994)

Teixeira, J. A., Mota, M., Venâncio, Armando

Cell-free and cell-occupied calcium alginate membranes were placed in a plate diffusion cell. The measurements of the diffusion coefficients for glucose and malic acid were performed for each...

Evaluation of crude hydroxypropyl starch as a bioseparation aqueous-phase-forming polymer (1993)

Venâncio, Armando, Teixeira, J. A., Mota, M.

A new aqueous two-phase system based on crude hydroxypropyl starch (HPS) and poly(ethy1ene glycol) (PEG) is described. Unlike other polymer-polymer aqueous twophase systems, the HPS used is...