Benami, A., Santana, G., Ortiz, A., Ponce, A., Romeu, D., Aguilar-Hernandez, J., ...
Strong white and blue photoluminescence (PL) from as-grown silicon nanocrystals (nc-Si) in SiNx films prepared by remote plasma enhanced chemical vapour deposition using SiCl4/NH3 mixtures is...
Thaw, P., Sedelnikova, S.E., Muranova, T., Wiese, S., Ayora, S., Alonso, J.C., ...
The Lrp/AsnC family of transcriptional regulatory proteins is found in both archaea and bacteria. Members of the family influence cellular metabolism in both a global (Lrp) and specific (AsnC)...
Characterization of two highly similar Rad51 homologs of Physcomitrella patens (2002)
Ayora,S., Piruat,J. I., Luna,R., Reiss,B., Russo,V. E. A., Aguilera,A., ...
The moss Physcomitrella patens, which is a land plant with efficient homologous recombination, encodes two Rad51 proteins PpaRad51.1 and PpaRad51.2). The PpaRad51.1 and PpaRad51.2 proteins, which...
Characterization of two highly similar Rad51 homologs of Physcomitrella patens (2002)
Ayora, S., Piruat, J. I., Luna, R., Reiss, B., Russo, V. E. A., Aguilera, A., ...
The moss Physcomitrella patens, which is a land plant with efficient homologous recombination, encodes two Rad51 proteins PpaRad51.1 and PpaRad51.2). The PpaRad51.1 and PpaRad51.2 proteins, which...
SEASONAL MOVEMENTS OF MALE GREAT BUSTARDS IN CENTRAL SPAIN (2001)
J. A. Alonso, C. A. Martín, J. C. Alonso, M. B. Morales, S. J. Lane
A sample of 22 radio-tagged male Great Bustards from central Iberian populations was studied from 1996 to 1998. All birds undertook seasonal movements to postbreeding areas located up to 167 km away...
Osborne, P.E., Alonso, J.C., Bryant, R.G.
1. Many species are adversely affected by human activities at large spatial scales and their conservation requires detailed information on distributions. Intensive ground surveys cannot keep pace...
Low Temperature SiO2 Films. (1998)
Falcony, C., Oritz, A., Lopez, S., Alonso, J. C., Muhl, S.
Silicon dioxide films have been deposited by the plasma-enhanced chemical vapor deposition technique using silicon halides (SiF4 and SiF4) and O2 or N2O as reactive materials. In the case of SiF4 a...
Proximate and ultimate causes of natal dispersal in the great bustard Otis tarda (1998)
Alonso, J. C., Martín, E., Alonso, J. A., Morales, M. B.
Between 1991 and 1997 we studied the offspring independence and juvenile dispersal in a wild population of great bustards (Otis tarda). Young males were independent and began their juvenile dispersal...
ALONSO, J.C., LÖPEZ-LONGO, F.J., LAMPREAVE, J.L., GONZÁLEZ, M., ALMOGUERA, L., CARREÑO, L.
The aim was to analyse the abdominal scintigraphy pattern in patients with seronegative spcondylarthropathy (Ssp), ulcerative colitis (UC) and Crohn's disease (CD). A total d 117 patients with...
Canosa, I, Rojo, F, Alonso, J C
The beta recombinase from the broad host range Grampositive plasmid pSM19035 catalyzes intramolecular site-specific recombination between two directly or inversely oriented recombination sites in the...
Purification and characterization of the RecF protein from Bacillus subtilis 168.
Genetic evidence suggests that the Bacillus subtilis recF gene product is involved in DNA repair and recombination. The RecF protein was overproduced and purified. NH2-terminal protein sequence...
DNA gyrase inhibitors block development of Bacillus subtilis bacteriophage SP01.
Alonso, J C, Sarachu, A N, Grau, O
SP01 development was inhibited by nalidixic acid and novobiocin in the sensitive host Bacillus subtilis 168M. Inhibition by novobiocin was prevented by a Novr mutation in the cellular DNA gyrase...
Sekiguchi, J, Akeo, K, Yamamoto, H, Khasanov, F K, Alonso, J C, Kuroda, A
DNA sequencing of a region upstream of the mms223 gene of Bacillus subtilis showed the presence of two open reading frames, orf1 and orf2, which may encode 18- and 27-kDa polypeptides, respectively....
Characterization of Bacillus subtilis recombinational pathways.
Alonso, J C, Lüder, G, Tailor, R H
Recombination in Bacillus subtilis requires the products of numerous rec loci. To dissect the various mechanisms which may be involved in genetic recombination, we constructed a series of isogenic...
Plasmid structural instability associated with pC194 replication functions.
Ballester, S, Lopez, P, Espinosa, M, Alonso, J C, Lacks, S A
The hybrid plasmid pJS37 is composed of the streptococcal plasmid pLS1, which confers tetracycline resistance, and the staphylococcal plasmid pC194, which confers chloramphenicol resistance. When...
Characterization of recombination-deficient mutants of Bacillus subtilis.
Alonso, J C, Tailor, R H, Lüder, G
An isogenic set of "prophage-free," DNA repair-proficient and -deficient strains of Bacillus subtilis were characterized phenotypically. The mutant strains were provisionally classified into four...
Deichelbohrer, I, Alonso, J C, Lüder, G, Trautner, T A
Any SPP1 DNA restriction fragment cloned into Bacillus subtilis plasmid pC194 or pUB110 increased the transduction frequency of the plasmid by SPP1 100- to 1,000-fold over the transduction level of...
Generation of linear multigenome-length plasmid molecules in Bacillus subtilis.
Linear multigenome-length double and single stranded plasmid DNA was identified in a Bacillus subtilis ATP-dependent DNAase mutant strain (addA5) bearing plasmids pC194 or pBD95ts. Plasmid pBC30, a...
Müller, A K, Rojo, F, Alonso, J C
Plasmids control their copy number by limiting the amount of the initiator for DNA replication. The plasmid pUB110 initiator protein is termed RepU. Expression of the pUB110 repU gene is controlled...
The beta recombinase from plasmid pSM19035 catalyzes intramolecular site-specific recombination between two directly or inversely oriented six sites in the presence of a chromatin-associated protein...
The beta protein encoded by the Streptococcus pyogenes plasmid pSM19035 is a site-specific recombinase involved in both resolution of plasmid multimers into monomers and DNA inversion. It has been...
Functional analysis of the Bacillus subtilis bacteriophage SPP1 pac site.
Bravo, A, Alonso, J C, Trautner, T A
Encapsidation of the DNA of the virulent Bacillus subtilis phage SPP1 follows a processive unidirectional headful-mechanism and initiates at a unique genomic location (pac). We have cloned a fragment...
Bacteriophage SPP1 infection of Bacillus subtilis cells bearing plasmids induces the synthesis of multigenome-length plasmid molecules. Two independent pathways can account for this synthesis. In one...
Brantl, S, Behnke, D, Alonso, J C
The large conjugative plasmid pIP501 was originally isolated from Streptococcus agalactiae. To study the molecular basis of pIP501 replication we determined the nucleotide sequence of a 2.2 kb DNA...
Alonso, J C, Shirahige, K, Ogasawara, N
In Bacillus subtilis the recM gene, whose product is associated with DNA repair and recombination, has been located between the dnaX and rrnA genes. The recM gene has been cloned and analyzed....
Leonhardt, H, Lurz, R, Alonso, J C
The synthesis and structure of linear multimeric plasmid molecules (hmw DNA) in Bacillus subtilis were investigated. The replication of covalently-closed-circular supercoiled (form I) DNA requires...
The origin of lagging strand synthesis in pUB110, oriL, has been localized within 140 bases outside the pUB110 minimal replicon. The oriL DNA sequence is a cis-acting and orientation dependent...
Functional analysis of the leading strand replication origin of plasmid pUB110 in Bacillus subtilis.
Alonso, J C, Leonhardt, H, Stiege, C A
Supercoiled plasmid DNA is the substrate for initiation of pUB110 replication, and - by inference - for binding of its initiator protein (RepU) to the plasmid replication origin (oriU) in vivo. No...
Recombination-dependent concatemeric plasmid replication.
Viret, J F, Bravo, A, Alonso, J C
The replication of covalently closed circular supercoiled (form I) DNA in prokaryotes is generally controlled at the initiation level by a rate-limiting effector. Once initiated, replication proceeds...
Alonso, J C, Lüder, G, Trautner, T A
We had previously proposed that the production of concatemeric plasmid DNA in plasmid-transducing SPP1 particles is a consequence of phage-directed rolling-circle-type replication of plasmid DNA. The...
Canosa, I, Rojo, F, Alonso, J C
The beta recombinase from the broad host range Grampositive plasmid pSM19035 catalyzes intramolecular site-specific recombination between two directly or inversely oriented recombination sites in the...
Purification and characterization of the RecF protein from Bacillus subtilis 168.
Genetic evidence suggests that the Bacillus subtilis recF gene product is involved in DNA repair and recombination. The RecF protein was overproduced and purified. NH2-terminal protein sequence...
DNA gyrase inhibitors block development of Bacillus subtilis bacteriophage SP01.
Alonso, J C, Sarachu, A N, Grau, O
SP01 development was inhibited by nalidixic acid and novobiocin in the sensitive host Bacillus subtilis 168M. Inhibition by novobiocin was prevented by a Novr mutation in the cellular DNA gyrase...
Sekiguchi, J, Akeo, K, Yamamoto, H, Khasanov, F K, Alonso, J C, Kuroda, A
DNA sequencing of a region upstream of the mms223 gene of Bacillus subtilis showed the presence of two open reading frames, orf1 and orf2, which may encode 18- and 27-kDa polypeptides, respectively....
Characterization of Bacillus subtilis recombinational pathways.
Alonso, J C, Lüder, G, Tailor, R H
Recombination in Bacillus subtilis requires the products of numerous rec loci. To dissect the various mechanisms which may be involved in genetic recombination, we constructed a series of isogenic...
Plasmid structural instability associated with pC194 replication functions.
Ballester, S, Lopez, P, Espinosa, M, Alonso, J C, Lacks, S A
The hybrid plasmid pJS37 is composed of the streptococcal plasmid pLS1, which confers tetracycline resistance, and the staphylococcal plasmid pC194, which confers chloramphenicol resistance. When...
Characterization of recombination-deficient mutants of Bacillus subtilis.
Alonso, J C, Tailor, R H, Lüder, G
An isogenic set of "prophage-free," DNA repair-proficient and -deficient strains of Bacillus subtilis were characterized phenotypically. The mutant strains were provisionally classified into four...
Deichelbohrer, I, Alonso, J C, Lüder, G, Trautner, T A
Any SPP1 DNA restriction fragment cloned into Bacillus subtilis plasmid pC194 or pUB110 increased the transduction frequency of the plasmid by SPP1 100- to 1,000-fold over the transduction level of...
Generation of linear multigenome-length plasmid molecules in Bacillus subtilis.
Linear multigenome-length double and single stranded plasmid DNA was identified in a Bacillus subtilis ATP-dependent DNAase mutant strain (addA5) bearing plasmids pC194 or pBD95ts. Plasmid pBC30, a...
Müller, A K, Rojo, F, Alonso, J C
Plasmids control their copy number by limiting the amount of the initiator for DNA replication. The plasmid pUB110 initiator protein is termed RepU. Expression of the pUB110 repU gene is controlled...
The beta recombinase from plasmid pSM19035 catalyzes intramolecular site-specific recombination between two directly or inversely oriented six sites in the presence of a chromatin-associated protein...
The beta protein encoded by the Streptococcus pyogenes plasmid pSM19035 is a site-specific recombinase involved in both resolution of plasmid multimers into monomers and DNA inversion. It has been...
Functional analysis of the Bacillus subtilis bacteriophage SPP1 pac site.
Bravo, A, Alonso, J C, Trautner, T A
Encapsidation of the DNA of the virulent Bacillus subtilis phage SPP1 follows a processive unidirectional headful-mechanism and initiates at a unique genomic location (pac). We have cloned a fragment...
Bacteriophage SPP1 infection of Bacillus subtilis cells bearing plasmids induces the synthesis of multigenome-length plasmid molecules. Two independent pathways can account for this synthesis. In one...
Brantl, S, Behnke, D, Alonso, J C
The large conjugative plasmid pIP501 was originally isolated from Streptococcus agalactiae. To study the molecular basis of pIP501 replication we determined the nucleotide sequence of a 2.2 kb DNA...
Alonso, J C, Shirahige, K, Ogasawara, N
In Bacillus subtilis the recM gene, whose product is associated with DNA repair and recombination, has been located between the dnaX and rrnA genes. The recM gene has been cloned and analyzed....
Leonhardt, H, Lurz, R, Alonso, J C
The synthesis and structure of linear multimeric plasmid molecules (hmw DNA) in Bacillus subtilis were investigated. The replication of covalently-closed-circular supercoiled (form I) DNA requires...
The origin of lagging strand synthesis in pUB110, oriL, has been localized within 140 bases outside the pUB110 minimal replicon. The oriL DNA sequence is a cis-acting and orientation dependent...
Functional analysis of the leading strand replication origin of plasmid pUB110 in Bacillus subtilis.
Alonso, J C, Leonhardt, H, Stiege, C A
Supercoiled plasmid DNA is the substrate for initiation of pUB110 replication, and - by inference - for binding of its initiator protein (RepU) to the plasmid replication origin (oriU) in vivo. No...
Recombination-dependent concatemeric plasmid replication.
Viret, J F, Bravo, A, Alonso, J C
The replication of covalently closed circular supercoiled (form I) DNA in prokaryotes is generally controlled at the initiation level by a rate-limiting effector. Once initiated, replication proceeds...
Alonso, J C, Lüder, G, Trautner, T A
We had previously proposed that the production of concatemeric plasmid DNA in plasmid-transducing SPP1 particles is a consequence of phage-directed rolling-circle-type replication of plasmid DNA. The...