Furesz, S E, Mallard, B A, Bossé, J T, Rosendal, S, Wilkie, B N, MacInnes, J I
Current porcine pleuropneumonia bacterins afford only partial protection by decreasing mortality but not morbidity. In order to better understand the type(s) of immune response associated with...
Genetic and biochemical analyses of Actinobacillus pleuropneumoniae urease.
The urease gene cluster from the virulent Actinobacillus pleuropneumoniae serotype 1 strain CM5 was cloned and sequenced. The urease activity was associated with a 6.3-kbp region which contains eight...
Bannan, J D, Moran, M J, MacInnes, J I, Soltes, G A, Friedman, R L
To determine whether hemolytic factors other than the bifunctional hemolysin-adenylate cyclase toxin (cyclolysin) are expressed by Bordetella pertussis, a gene library was constructed from a virulent...
Actinobacillus pleuropneumoniae hlyX gene homology with the fnr gene of Escherichia coli.
MacInnes, J I, Kim, J E, Lian, C J, Soltes, G A
The hlyX gene from Actinobacillus pleuropneumoniae, which confers a hemolytic phenotype on Escherichia coli, was sequenced, and its role in regulation of gene expression was investigated. No...
Van Ostaaijen, J, Frey, J, Rosendal, S, MacInnes, J I
Actinobacillus suis isolates recovered from both healthy and diseased pigs were characterized by biochemical testing, serotyping, restriction endonuclease fingerprinting, and apx toxin gene typing....
Lian, C J, Rosendal, S, MacInnes, J I
This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...
Analysis of major antigens of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms.
Outer membrane protein (OMP)-enriched extracts and whole-cell protein preparations of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel...
Tissue-specific expression of the rat alpha 2u globulin gene family.
MacInnes, J I, Nozik, E S, Kurtz, D T
The rat alpha 2u globulin gene family encodes approximately 20 low-molecular-weight (20,000) proteins with pIs ranging from 4.5 to 7.9. alpha 2u globulin protein isoforms were detected in the liver...
In recent years, Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis have emerged as important pathogens of swine, particularly in high health status herds. Their association with a...
Comparative pathogenicity of different Actinobacillus suis O/K serotypes.
Slavić, D, DeLay, J, Hayes, M A, MacInnes, J I
The pathogenicity of Actinobacillus suis serotypes O1/K1 (strain SO4), O1/K2 (strain C84), and O2/K2 (strain H91-0380) was evaluated in specific-pathogen-free (SPF) piglets challenged by...
The contribution of urease activity to the pathogenesis of Actinobacillus pleuropneumoniae was investigated using 2 different urease-negative transposon mutants of the virulent serotype 1 strain, CM5...
Smart, N L, Miniats, O P, MacInnes, J I
To study the occurrence and distribution of various strains of Haemophilus parasuis in southern Ontario swine, organisms isolated from healthy swine, from specific pathogen-free and conventional...
Experimental sialodacryoadenitis virus infection in athymic CD-1 mice.
Percy, D H, Bond, S J, MacInnes, J I, Descôteaux, J P
The purpose of the study was to investigate the susceptibility of nude mice to sialodacryoadenitis virus. Young adult male CD-1 nude mice were inoculated intranasally with virus, killed at 2, 4, 6,...
MacInnes, J I, Borr, J D, Massoudi, M, Rosendal, S
Isolates of Actinobacillus (Haemophilus) pleuropheumoniae were studied by restriction endonuclease fingerprinting (REF) analysis using the enzymes BamHI and HindIII. Restriction fragments were...
Barker, M G, Percy, D H, Hovland, D J, MacInnes, J I
A procedure was developed for the partial purification of the rat coronaviruses, sialodacryoadenitis virus (SDAV) and Parker's rat coronavirus (PRC). The SDAV and PRC were replicated in L-2 cell...
Furesz, S E, Mallard, B A, Bossé, J T, Rosendal, S, Wilkie, B N, MacInnes, J I
Current porcine pleuropneumonia bacterins afford only partial protection by decreasing mortality but not morbidity. In order to better understand the type(s) of immune response associated with...
Genetic and biochemical analyses of Actinobacillus pleuropneumoniae urease.
The urease gene cluster from the virulent Actinobacillus pleuropneumoniae serotype 1 strain CM5 was cloned and sequenced. The urease activity was associated with a 6.3-kbp region which contains eight...
Bannan, J D, Moran, M J, MacInnes, J I, Soltes, G A, Friedman, R L
To determine whether hemolytic factors other than the bifunctional hemolysin-adenylate cyclase toxin (cyclolysin) are expressed by Bordetella pertussis, a gene library was constructed from a virulent...
Actinobacillus pleuropneumoniae hlyX gene homology with the fnr gene of Escherichia coli.
MacInnes, J I, Kim, J E, Lian, C J, Soltes, G A
The hlyX gene from Actinobacillus pleuropneumoniae, which confers a hemolytic phenotype on Escherichia coli, was sequenced, and its role in regulation of gene expression was investigated. No...
Van Ostaaijen, J, Frey, J, Rosendal, S, MacInnes, J I
Actinobacillus suis isolates recovered from both healthy and diseased pigs were characterized by biochemical testing, serotyping, restriction endonuclease fingerprinting, and apx toxin gene typing....
Lian, C J, Rosendal, S, MacInnes, J I
This article describes the molecular cloning and expression of a hemolysin gene from a serotype 1 strain of Actinobacillus pleuropneumoniae. The hemolysin was a thermolabile protein with an apparent...
Analysis of major antigens of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms.
Outer membrane protein (OMP)-enriched extracts and whole-cell protein preparations of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel...
Tissue-specific expression of the rat alpha 2u globulin gene family.
MacInnes, J I, Nozik, E S, Kurtz, D T
The rat alpha 2u globulin gene family encodes approximately 20 low-molecular-weight (20,000) proteins with pIs ranging from 4.5 to 7.9. alpha 2u globulin protein isoforms were detected in the liver...
In recent years, Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis have emerged as important pathogens of swine, particularly in high health status herds. Their association with a...
Comparative pathogenicity of different Actinobacillus suis O/K serotypes.
Slavić, D, DeLay, J, Hayes, M A, MacInnes, J I
The pathogenicity of Actinobacillus suis serotypes O1/K1 (strain SO4), O1/K2 (strain C84), and O2/K2 (strain H91-0380) was evaluated in specific-pathogen-free (SPF) piglets challenged by...
The contribution of urease activity to the pathogenesis of Actinobacillus pleuropneumoniae was investigated using 2 different urease-negative transposon mutants of the virulent serotype 1 strain, CM5...
Smart, N L, Miniats, O P, MacInnes, J I
To study the occurrence and distribution of various strains of Haemophilus parasuis in southern Ontario swine, organisms isolated from healthy swine, from specific pathogen-free and conventional...
Experimental sialodacryoadenitis virus infection in athymic CD-1 mice.
Percy, D H, Bond, S J, MacInnes, J I, Descôteaux, J P
The purpose of the study was to investigate the susceptibility of nude mice to sialodacryoadenitis virus. Young adult male CD-1 nude mice were inoculated intranasally with virus, killed at 2, 4, 6,...
MacInnes, J I, Borr, J D, Massoudi, M, Rosendal, S
Isolates of Actinobacillus (Haemophilus) pleuropheumoniae were studied by restriction endonuclease fingerprinting (REF) analysis using the enzymes BamHI and HindIII. Restriction fragments were...
Barker, M G, Percy, D H, Hovland, D J, MacInnes, J I
A procedure was developed for the partial purification of the rat coronaviruses, sialodacryoadenitis virus (SDAV) and Parker's rat coronavirus (PRC). The SDAV and PRC were replicated in L-2 cell...