Rötzschke, O., Falk, K., Mack, J., Lau, J. M., Jung, G., Strominger, J. L.
Class II MHC molecules are known to exist in conformational variants. “Floppy” and “compact” forms of murine MHC molecules, for example, are discriminated by their migration behavior on...
Multiple receptors for HLA-G on human natural killer cells
Mandelboim, O., Pazmany, L., Davis, D. M., Valés-Gómez, M., Reyburn, H. T., Rybalov, B., ...
HLA-G is the putative natural killer (NK) cell inhibitory ligand expressed on the extravillous cytotrophoblast of the human placenta. Killing of the class I negative human B cell line 721.221 by NK...
Developmental plasticity of CNS microglia
Santambrogio, L., Belyanskaya, S. L., Fischer, F. R., Cipriani, B., Brosnan, C. F., Ricciardi-Castagnoli, P., ...
Microglia arise from CD45+ bone marrow precursors that colonize the fetal brain and play a key role in central nervous system inflammatory conditions. We report that parenchymal microglia are...
Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain.
Joung, I, Strominger, J L, Shin, J
A novel human cDNA encoding a cytosolic 62-kDa protein (p62) that binds to the Src homology 2 (SH2) domain of p56lck in a phosphotyrosine-independent manner has been cloned. The cDNA is composed of...
cAMP inducibility of transcriptional repressor ICER in developing and mature human T lymphocytes.
Bodor, J, Spetz, A L, Strominger, J L, Habener, J F
Stimulation of the cAMP-dependent signaling pathway exerts an inhibitory effect on the proliferation and effector functions of T cells. The ability of T cells to form high intracellular levels of...
Jardetzky, T S, Brown, J H, Gorga, J C, Stern, L J, Urban, R G, Strominger, J L, ...
The structure of the human major histocompatibility complex (MHC) class II molecule HLA-DR1 derived from the human lymphoblastoid cell line LG-2 has been determined in a complex with the...
Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution.
Tiedemann, R E, Urban, R J, Strominger, J L, Fraser, J D
Mutational studies indicate that the superantigen staphylococcal enterotoxin A (SEA) has two separate binding sites for major histocompatibility complex (MHC) class II molecules. Direct evidence is...
Park, I, Chung, J, Walsh, C T, Yun, Y, Strominger, J L, Shin, J
A previously undescribed 62-kDa protein (p62) that does not contain phosphotyrosine but, nevertheless, binds specifically to the isolated src homology 2 (SH2) domain of p56lck has been identified....
Wucherpfennig, K W, Yu, B, Bhol, K, Monos, D S, Argyris, E, Karr, R W, ...
Human T-cell-mediated autoimmune diseases are genetically linked to particular alleles of MHC class II genes. Susceptibility to pemphigus vulgaris (PV), an autoimmune disease of the skin, is linked...
Schaefer, B C, Strominger, J L, Speck, S H
The Epstein-Barr virus-encoded nuclear antigen EBNA-1 gene promoter for the restricted Epstein-Barr virus (EBV) latency program operating in group I Burkitt lymphoma (BL) cell lines was previously...
Wucherpfennig, K W, Hafler, D A, Strominger, J L
T-cell receptors (TCRs) recognize peptide bound within the relatively conserved structural framework of major histocompatibility complex (MHC) class I or class II molecules but can discriminate...
Joung, I, Kim, T, Stolz, L A, Payne, G, Winkler, D G, Walsh, C T, ...
During T-cell activation, Ser59 in the unique N-terminal region of p56lck is phosphorylated. Mutation of Ser59 to Glu59 mimics Ser59 phosphorylation, and upon CD4 crosslinking, this mutant p56lck...
Regulation of T-cell antigen receptor (TCR) alpha-chain expression by TCR beta-chain transcripts.
The TCR is an alpha beta heterodimer, a part of the multimeric structure through which physiological T-cell activation occurs. The expression of TCR alpha chain is greatly diminished in a...
A subset of HLA-B27 molecules contains peptides much longer than nonamers.
Urban, R G, Chicz, R M, Lane, W S, Strominger, J L, Rehm, A, Kenter, M J, ...
An unusual monoclonal antibody (MARB4) directed against HLA-B27 that reacts with only approximately 5-20% of the cell surface HLA-B27 was used for large-scale purification of these molecules....
Tumor necrosis factor alpha is an autocrine growth factor for normal human B cells.
Boussiotis, V A, Nadler, L M, Strominger, J L, Goldfeld, A E
Transcription of the human tumor necrosis factor alpha (TNF-alpha) gene is one of the earliest events that occurs after stimulation of B or T cells via their antigen receptors. Antibody directed at...
Hilyard, K L, Reyburn, H, Chung, S, Bell, J I, Strominger, J L
An Escherichia coli expression system has been developed to produce milligram quantities of the variable domains of a human T-cell receptor from a cytotoxic T cell that recognizes the...
Hedley, M L, Urban, R G, Strominger, J L
In vitro transcription/translation of HLA-DR1 cDNAs in the presence of microsomal membranes was used to study the association of major histocompatibility complex class II molecules with peptide and...
Functional three-domain single-chain T-cell receptors.
Chung, S, Wucherpfennig, K W, Friedman, S M, Hafler, D A, Strominger, J L
T-cell receptors (TCRs) are membrane anchored heterodimers structurally related to antibody molecules. Single-chain antibodies can be engineered by linking the two variable domains, which fold...
CD43, the major sialoglycoprotein of human leukocytes, whose expression is defective in patients with the Wiskott-Aldrich syndrome, was down-regulated by phorbol 12-myristate 13-acetate (PMA) on...
Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck.
Winkler, D G, Park, I, Kim, T, Payne, N S, Walsh, C T, Strominger, J L, ...
Ser-42 and Ser-59 in the N-terminal region have been identified as the major phorbol ester-induced phosphorylation sites of p56lck. Phosphorylation of Ser-59 results in a gel shift from 56 kDa to 61...
Guo, H C, Madden, D R, Silver, M L, Jardetzky, T S, Gorga, J C, Strominger, J L, ...
Coordinates from x-ray structures of HLA-A*6801, HLA-A*0201, and HLA-B*2705 were analyzed to examine the basis for their selectivity in peptide binding. The pocket that binds the side chain of the...
Brooks, E G, Balk, S P, Aupeix, K, Colonna, M, Strominger, J L, Groh-Spies, V
Most human T cells express the TCR alpha/beta and either CD4 or CD8 molecules (single positive, SP); however, small numbers lack CD4 and CD8. In inbred mice, alpha/beta CD4-CD8- (double negative, DN)...
Colonna, M, Borsellino, G, Falco, M, Ferrara, G B, Strominger, J L
Natural killer (NK) cells recognize alloantigens on normal cells. One of these alloantigens correlates with homozygosity for a dimorphism of HLA-C at positions 77-80, which is shared by a number of...
Allele-specific peptide ligand motifs of HLA-C molecules.
Falk, K, Rötzschke, O, Grahovac, B, Schendel, D, Stevanović, S, Gnau, V, ...
The consensus motifs of HLA-Cw3, -Cw4, -Cw6, and -Cw7 ligands were determined by pool sequencing. Together with information obtained by sequencing of some prominent individual peptides, the results...
Zinc regulates the function of two superantigens.
Fraser, J D, Urban, R G, Strominger, J L, Robinson, H
Staphylococcal enterotoxins bind with high affinity to class II major histocompatibility complex proteins and subsequently stimulate large numbers of T cells via the V beta portion of the T-cell...
Disruption of the CD4-p56lck complex is required for rapid internalization of CD4.
Sleckman, B P, Shin, J, Igras, V E, Collins, T L, Strominger, J L, Burakoff, S J
CD4 is a cell surface glycoprotein expressed by a subset of T lymphocytes and functions to enhance T-cell activation. CD4 is noncovalently associated via the cytoplasmic domain with the...
Colonna, M, Spies, T, Strominger, J L, Ciccone, E, Moretta, A, Moretta, L, ...
Human natural killer (NK) cells with the CD3- CD16+ phenotype recognize allospecificities on normal T-cell blasts. The NK-defined specificity 1 (NK-1) is recessively inherited and has been mapped to...
Goldfeld, A E, Flemington, E K, Boussiotis, V A, Theodos, C M, Titus, R G, Strominger, J L, ...
The human tumor necrosis factor alpha (TNF-alpha) gene encodes a cytokine whose activities have been implicated in many immunopathological processes, including the activation and differentiation of...
Shin, J, Dunbrack, R L, Lee, S, Strominger, J L
A mutant of CD4 (CD4.Q421stop), in which the cytoplasmic C-terminal 13 amino acids were truncated, was not expressed on the surface of HeLa cells after transfection but was retained in the...
Woisetschlaeger, M, Jin, X W, Yandava, C N, Furmanski, L A, Strominger, J L, Speck, S H
During latent Epstein-Barr virus (EBV) infection of human B lymphocytes, six viral nuclear antigen (EBNAs) are expressed from long primary transcripts by means of alternative splicing and alternative...
Expression of class II major histocompatibility complex antigens in defective B-lymphoblastoid cell lines from patients with class II antigen deficiency and from in vitro mutants generated with the...
Ono, S J, Bazil, V, Levi, B Z, Ozato, K, Strominger, J L
Transcriptional regulation of the human major histocompatibility complex class II genes requires at least two upstream elements, the X and Y boxes, located in the -50- to -150-base-pair region of all...
Ono, S J, Liou, H C, Davidon, R, Strominger, J L, Glimcher, L H
A complementary DNA encoding a member of the leucine-zipper class of proteins (human X-box-binding protein, hXBP-1) that binds to the 3' end of the conserved X box (X2) of the HLA-DRA major...
Schaefer, B C, Woisetschlaeger, M, Strominger, J L, Speck, S H
Epstein-Barr virus transformation of human B lymphocytes in vitro results in the expression of six viral nuclear antigens (EBNAs) and three viral membrane proteins. However, examination of viral gene...
Bahram, S, Arnold, D, Bresnahan, M, Strominger, J L, Spies, T
The class II region of the human major histocompatibility complex (MHC) may encode several genes controlling the processing of endogenous antigen and the presentation of peptide epitopes by MHC class...
Sugawara, M, Ponath, P D, Shin, J, Yang, Z, Strominger, J L
The cis-acting DNA sequences that control expression of the HLA-DPA and HLA-DQB promoters have been investigated in detail. A set of recombinant plasmids containing 5' promoter deletions as well as...
Positioning of a peptide in the cleft of HLA-A2 by complementing amino acid changes.
Latron, F, Moots, R, Rothbard, J B, Garrett, T P, Strominger, J L, McMichael, A
Several mutant HLA-A2 molecules have been constructed and expressed in the mutant human B-cell line C1R, which lacks HLA-A and HLA-B antigens, and examined for presentation of a previously defined...
Promoter switching in Epstein-Barr virus during the initial stages of infection of B lymphocytes.
Woisetschlaeger, M, Yandava, C N, Furmanski, L A, Strominger, J L, Speck, S H
Transcription of the genes encoding the six viral nuclear antigens present in Epstein-Barr virus latently infected lymphocytes can be initiated from one of two promoters (Cp and Wp) mapping near the...
Banerji, J, Sands, J, Strominger, J L, Spies, T
A large number of genes has been identified previously between the class I and class II gene families within the class III region of the human major histocompatibility complex. The complete sequences...
Regulation of the class II-associated invariant chain gene in normal and mutant B lymphocytes.
Doyle, C, Ford, P J, Ponath, P D, Spies, T, Strominger, J L
The invariant chain protein is intracellularly associated with class II major histocompatibility proteins. In many cases, the expression of these molecules appears to be regulated in a similar...
Maturation or differentiation of human thymocyte precursors in vitro?
Groh, V, Fabbi, M, Strominger, J L
The differentiation or maturation potential of human thymocyte precursors has been studied by using a population of CD3/TCR-, CD4-, CD8- ("triple negative") thymocytes isolated by negative selection...
Interferon gamma response region in the promoter of the human DPA gene.
Yang, Z, Sugawara, M, Ponath, P D, Wessendorf, L, Banerji, J, Li, Y, ...
The interferon gamma (IFN-gamma) response region of the human class II major histocompatibility complex gene, DPA, has been localized to a 52-base-pair (bp) DNA fragment in the proximal promotor at...
Stienekemeier, M., Falk, K., Rötzschke, O., Weishaupt, A., Schneider, C., Toyka, K. V., ...
Using a polypeptide oligomer harboring 16 repeats of the neuritogenic epitope (aa 58–73) of myelin P2 protein separated by spacers, enhancement of the immune response to the P2 protein, an...
Sedimentation characteristics of newly synthesized Epstein-Barr viral DNA in superinfected cells.
Siegel, P J, Clough, W, Strominger, J L
Replicating Epstein-Barr virus (EBV) DNA molecules isolated from superinfected Raji cells were shown to consist of 80S to 65S and 58S (mature) molecules Pulse-chase experiments showed that...
Penicillin-binding proteins in Bacillus subtilis mutants.
Kleppe, G, Yu, W, Strominger, J L
The penicillin-binding proteins (PBPs) from mutants of Bacillus subtilis were studied and related to morphology. In a previously described cloxacillin-resistant mutant of B. subtilis strain Porton,...
Macaques immunized with HLA-DR are protected from challenge with simian immunodeficiency virus.
Arthur, L O, Bess, J W, Urban, R G, Strominger, J L, Morton, W R, Mann, D L, ...
Macaques immunized with uninfected human cells have been shown to be protected from challenge with simian immunodeficiency virus (SIV) propagated in human cells. To identify the potential antigens...
Schaefer, B C, Strominger, J L, Speck, S H
The Epstein-Barr virus BamHI F promoter (Fp) was previously identified as the putative EBNA 1 gene promoter in group 1 Burkitt's lymphoma (BL) cell lines. Fp has also been shown to be activated in...
Borras, A M, Strominger, J L, Speck, S H
Induction of the Epstein-Barr virus lytic cycle is mediated through the immediate-early BZLF1 gene and the coordinately regulated BRLF1 gene. The BZLF1 gene product, Zta, transactivates its own...
Schaefer, B C, Strominger, J L, Speck, S H
In Epstein-Barr virus (EBV)-associated tumors that arise in immunocompetent individuals, the pattern of viral gene expression is very restricted compared with that of latently infected B cells in...
Identification of the active site in penicillin-binding protein 3 of Escherichia coli.
Nicholas, R A, Strominger, J L, Suzuki, H, Hirota, Y
We report the sequence of the active site tryptic peptide of penicillin-binding protein 3 from Escherichia coli. Purified penicillin-binding protein 3 was labeled with [14C]penicillin G and digested...
Penicillin-binding proteins (PBPs) 5 and 6 of Escherichia coli released the bound penicilloyl moiety at an intermediate rate relative to, e.g., Staphylococcus aureus PBPs 4 (rapid) and 1 or 2 (slow)....
Purified penicillin-binding protein 1a of Escherichia coli formed an acyl enzyme intermediate with the highly reactive synthetic substrate diacetyl-L-lysyl-D-alanyl-D-lactate at acid pH, although in...
High-molecular-weight penicillin-binding proteins from membranes of bacilli.
Waxman, D J, Lindgren, D M, Strominger, J L
Mixtures of high-molecular-weight, cephalosporin-sensitive penicillin-binding proteins (PBPs) can be purified from Bacillus subtilis membranes by cephalosporin affinity chromatography (G. Kleppe and...
Penicillin is an active-site inhibitor for four genera of bacteria.
Yocum, R R, Amanuma, H, O'Brien, T A, Waxman, D J, Strominger, J L
The hypothesis that penicillin acts as an active-site inhibitor cell wall biosynthesis was tested by a method of partial proteolytic mapping of penicillin-binding sites versus substrate-binding sites...
Purification of penicillin-binding protein 2 of Escherichia coli.
Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose...
Matsuhashi, M, Tamaki, S, Curtis, S J, Strominger, J L
The defect in D-alanine carboxypeptidase IA activity in the dacA11191 mutant of Escherichia coli was correlated with a defect in the release of penicillin G from penicillin-binding protein 5. The...
Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape.
Iwaya, M, Goldman, R, Tipper, D J, Feingold, B, Strominger, J L
Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells...
Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli.
Iwaya, M, Jones, C W, Khorana, J, Strominger, J L
Genes responsible for round morphology in mecillinam-resistant, round morphological mutants of Escherichia coli have been mapped. Three mutants, called rodX, mapped at around 14 min, and two, called...
STRUCTURE OF THE PEPTIDOGLYCAN OF BACTERIAL SPORES: OCCURRENCE OF THE LACTAM OF MURAMIC ACID*
Warth, A. D., Strominger, J. L.
Six major oligosaccharides were released from the peptidoglycan of spores of Bacillus subtilis by lysozyme treatment. They were isolated and characterized as a disaccharide, tetrasaccharide, and...
Schaefer, B C, Strominger, J L, Speck, S H
Epstein-Barr virus (EBV) is capable of adopting three distinct forms of latency: the type III latency program, in which six EBV-encoded nuclear antigens (EBNAs) are expressed, and the type I and type...
Schaefer, B C, Paulson, E, Strominger, J L, Speck, S H
The Epstein-Barr virus (EBV) EBNA1 gene promoter active in the type I program of restricted viral latency was recently identified and shown to reside in the viral BamHI Q fragment. This promoter, Qp,...
Mapping of a new genetic locus responsible for ampicillin resistance in Escherichia coli.
Buchanan, C E, Strominger, J L
The ampicillin resistance locus of three different ampicillin-resistant, temperature-sensitive Escherichia coli mutants was mapped between proC and purE and does not correspond to any of the known...
Electron microscope studies of conditional spore cortexless mutants of Bacillus sphaericus.
Imae, Y, Strominger, M B, Strominger, J L
Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid...
Relationship between cortex content and properties of Bacillus sphaericus spores.
The muramic lactam content of spores of Bacillus sphaericus mutants defective in meso-diaminopimelic acid synthesis increases almost linearly with an increase of meso-diaminopimelic acid...
Cortex content of asporogenous mutants of Bacillus subtilis.
A method for the measurement of muramic lactam, which is specifically located in the cortical peptidoglycan of bacterial spores, was developed as a quantitative assay method for spore cortex content....
Protein and Carbohydrate Composition of the Cell Envelope of Halobacterium salinarium1
Mescher, M. F., Strominger, J. L., Watson, S. W.
The isolated cell envelope of Halobacterium salinarium strain 1 contained 15 to 20 proteins that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. All but...
Skare, J, Farley, J, Strominger, J L, Fresen, K O, Cho, M S, Zur Hausen, H
Eight independent recombinant Epstein-Barr virus genomes, each of which was a transforming strain, were made by superinfecting cell lines containing Epstein-Barr virus DNA (Raji or B95-8 strain) with...
Pfitzner, A J, Tsai, E C, Strominger, J L, Speck, S H
The Epstein-Barr virus (EBV) mutant P3HR1 is incapable of immortalizing B lymphocytes because of a 6.8-kilobase deletion in the BamHI W, Y, and H regions of the viral genome (M. Rabson, L....
Pfitzner, A J, Strominger, J L, Speck, S H
A 1.95-kilobase cDNA clone was isolated by screening a size-selected lambda gt10 cDNA library prepared from an Epstein-Barr virus-transformed B-cell line, IB4, with the Epstein-Barr virus BamHI M...
The B95-8 isolate of Epstein-Barr virus arose from an isolate with a standard genome.
Skare, J, Edson, C, Farley, J, Strominger, J L
Blot hybridization studies revealed that the deletion which characterizes the DNA from the B95-8 strain of Epstein-Barr virus was not present in the virus from which the B95-8 strain was derived...
Latron, F, Jotterand-Bellomo, M, Maffei, A, Scarpellino, L, Bernard, M, Strominger, J L, ...
Constitutive expression of major histocompatibility complex class II genes is acquired very early in B-cell ontogeny and is maintained up to the B-cell blast stage. Terminal differentiation in plasma...
Austin, P J, Flemington, E, Yandava, C N, Strominger, J L, Speck, S H
Several cDNA clones containing the Epstein-Barr virus BamHI fragment H rightward open reading frame 1 (BHRF1) have been recovered from the tightly latent lymphoblastoid cell line IB4. These clones...
A 95-base-pair immediate upstream sequence of the human class II major histocompatibility complex DQB gene containing the conserved X and Y elements showed enhancer activity in a transient expression...
Genomic organization of the human T-cell antigen-receptor alpha/delta locus.
Satyanarayana, K, Hata, S, Devlin, P, Roncarolo, M G, De Vries, J E, Spits, H, ...
Two clusters of overlapping cosmid clones comprising about 100 kilobases (kb) at the human T-cell antigen-receptor alpha/delta locus were isolated from a genomic library. The structure of the...
McMichael, A J, Gotch, F M, Santos-Aguado, J, Strominger, J L
Cytotoxic T lymphocytes (CTL) specific for influenza A virus were prepared from 15 donors. Those with HLA-A2 recognized autologous or HLA-A2-matched B-lymphoblastoid cells in the presence of...
Dietzler, D. N., Strominger, J. L.
The enzyme which catalyzes the transfer of glucosyl residues from adenosine diphospho(ADP)-glucose to glycogen has been partially purified from extracts of Pasteurella pseudotuberculosis. In contrast...
Identification of murine complement receptor type 2.
Fingeroth, J D, Benedict, M A, Levy, D N, Strominger, J L
A rabbit antiserum reactive with the human complement component C3d/Epstein-Barr virus receptor (complement receptor type 2, CR2) immunoprecipitates a Mr 155,000 murine B-cell surface antigen. The...
Gorga, J C, Dong, A, Manning, M C, Woody, R W, Caughey, W S, Strominger, J L
We have examined the secondary structures of human class I and class II histocompatibility antigens in solution by Fourier transform infrared spectroscopy and circular dichroism in order to compare...
HLA class I antigens present in the human leukemia T-cell line HPB-ALL are shown to be endocytosed in the absence of specific antibodies. In 1 hr, approximately 10% of class I molecules initially...
Groh, V, Fabbi, M, Hochstenbach, F, Maziarz, R T, Strominger, J L
Double-negative (CD4-CD8-) T-cell receptor alpha- and beta-chain (TCR alpha beta)-expressing lymphocytes have been identified in human skin. Two skin-derived lymphocyte cell lines (II-1 and II-2)...
Mutually exclusive use of viral promoters in Epstein-Barr virus latently infected lymphocytes.
Woisetschlaeger, M, Strominger, J L, Speck, S H
Of the eight viral antigens known to be expressed during Epstein-Barr virus latency, six are transcribed from a major rightward transcriptional unit, which gives rise to mRNAs containing common 5'...
Alloreactivity studied with mutants of HLA-A2.
Santos-Aguado, J, Crimmins, M A, Mentzer, S J, Burakoff, S J, Strominger, J L
Based on the crystal structure of HLA-A2.1 and the recognition of a panel of mutant HLA-A2.1 molecules by a large number of alloreactive cytotoxic T lymphocyte clones, a model to explain...
Spies, T, Bresnahan, M, Strominger, J L
A 600-kilobase (kb) DNA segment from the human major histocompatibility complex (MHC) class III region was isolated by extension of a previous 435-kb chromosome walk. The contiguous series of cloned...
Quackenbush, E, Clabby, M, Gottesdiener, K M, Barbosa, J, Jones, N H, Strominger, J L, ...
Complementary DNA (cDNA) clones encoding the heavy chain of the heterodimeric human membrane glycoprotein 4F2 have been isolated by immunoscreening of a lambda gt11 expression library. The identity...
Linkage map of the human major histocompatibility complex including the tumor necrosis factor genes.
Carroll, M C, Katzman, P, Alicot, E M, Koller, B H, Geraghty, D E, Orr, H T, ...
The tumor necrosis factor (TNF) alpha and beta gene pair has been linked in the human major histocompatibility complex to HLA-B, HLA-C, and, tentatively, HLA-E and HLA-A on one side and to the class...
Huang, H J, Jones, N H, Strominger, J L, Herzenberg, L A
We report the isolation of cDNA clones of the mouse lymphocyte differentiation antigen Ly-1. One of these cDNA clones was confirmed to be full-length by DNA sequencing and by expression of Ly-1 by L...
Expression of human class II major histocompatibility complex antigens using retrovirus vectors.
Korman, A J, Frantz, J D, Strominger, J L, Mulligan, R C
Retrovirus vectors [direct orientation (DO) vectors] that permit the simultaneous expression of an inserted protein-coding sequence and a dominant-acting selectable marker have been constructed. In...
Takada, Y, Strominger, J L, Hemler, M E
The very late antigen (VLA) protein family contains at least five related heterodimers, including a fibronectin receptor structure, and probably other cell substrate adhesion receptors. These...
Krangel, M S, Bierer, B E, Devlin, P, Clabby, M, Strominger, J L, McLean, J, ...
The T-cell receptor (TCR) gamma gene product occurs in association with T3 (CD3) polypeptides on the surface of human T lymphocytes. TCR gamma lymphocytes express arrays of T3 polypeptides distinct...
Miwa, K, Doyle, C, Strominger, J L
All class II major histocompatibility complex genes contain two highly conserved sequences, termed X and Y, within the promoter regions(s), which may have a role in regulation of expression. To study...
Biochemical Studies of the Smooth-Rough Mutation in Salmonella minnesota
Lüderitz, O., Risse, H. J., Schulte-Holthausen, H., Strominger, J. L., Sutherland, I. W., Westphal, O.
Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the...
A method is described for scanning relatively large fragments of DNA for sequences which bind nuclear factors. This method was used to identify an octamer (ATTTGTAT) in the DQ beta gene upstream...
Irigoyen, O H, Rizzolo, P V, Thomas, Y, Hemler, M E, Shen, H H, Friedman, S M, ...
A monoclonal antibody, PVR-11, was obtained after hybridization of X63Ag8.653 murine myeloma cells with spleen cells from a mouse immunized with human lymphocytes. It recognizes a 175,000- to...
Human T cell surface antigens bearing a structural relationship to HLA antigens.
Cotner, T, Mashimo, H, Kung, P C, Goldstein, G, Strominger, J L
Three cell surface antigens that are structurally related to the human major histocompatibility antigens (called HLA antigens) have been characterized from the leukemic T cell line MOLT-4. One...
Mulder, A, Alexander, S, Engelfriet, C P, Von Dem Borne, A E, Strominger, J L
The human promyelocytic leukemia cell line HL-60 is reactive with an antiserum raised against normal human granulocytes (AGS). Immunoprecipitation with AGS on [35S]methionine-labeled HL-60 cell...
Collins, T, Lapierre, L A, Fiers, W, Strominger, J L, Pober, J S
Recombinant human tumor necrosis factor (TNF), purified to greater than 99% homogeneity, increases surface expression of class I major histocompatibility complex (MHC) antigens to a maximum of 9-fold...
Dialynas, D P, Murre, C, Quertermous, T, Boss, J M, Leiden, J M, Seidman, J G, ...
Complementary DNA (cDNA) encoding a human T-cell gamma chain has been cloned and sequenced. At the junction of the variable and joining regions, there is an apparent deletion of two nucleotides in...
Generation of diversity of the beta chain of the human T-lymphocyte receptor for antigen.
Human T-cell receptor beta-chain (Ti beta) genes are formed by the rearrangement of variable (V), diversity (D), and joining (J) gene segments. A comparison of the nucleotide and deduced amino acid...
Schamboeck, A, Korman, A J, Kamb, A, Strominger, J L
A total of 5724 base pairs of a recombinant phage DNA containing a human HLA-DR heavy chain gene including flanking regions has been analyzed. The regions corresponding to all the exons have been...
Terhorst, C, Parham, P, Mann, D L, Strominger, J L
Amino-acid and carbohydrate compositions in addition to limited NH2-terminal sequence data of four preparations of HLA antigens, including products of both of the major histocompatibility loci (HLA-A...
Morton, C C, Kirsch, I R, Nance, W E, Evans, G A, Korman, A J, Strominger, J L
We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization. Our data indicate that a cloned...
Identification and mapping of polypeptides encoded by the P3HR-1 strain of Epstein-Barr virus.
Cohen, L K, Speck, S H, Roberts, B E, Strominger, J L
The Epstein-Barr virus (EBV)-specified polypeptides induced upon viral replication in the P3HR-1 cell line have been examined by immunoprecipitation with a high-titer human anti-EBV serum....
Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells.
Hemler, M E, Brenner, M B, McLean, J M, Strominger, J L
Antigen-specific, interleukin 2 (IL-2)-dependent human T-cell lines and clones were utilized to study the relationship between IL-2 receptor expression and antigenic stimulation. T cells that had not...
Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2.
Fingeroth, J D, Weis, J J, Tedder, T F, Strominger, J L, Biro, P A, Fearon, D T
Identity of the Epstein-Barr virus (EBV) receptor with the complement receptor type 2 (CR2) was established in three sets of experiments using the monoclonal antibodies, HB-5 and anti-B2, which...
Korman, A J, Knudsen, P J, Kaufman, J F, Strominger, J L
A monoclonal antibody (HC 2.1) directed against the separated heavy chain of HLA-DR has been prepared. By binding HC 2.1 to polysomes from human B lymphoblastoid cells followed by the use of a...
Long-term human cytolytic T-cell lines allospecific for HLA-DR6 antigen are OKT4+.
Krensky, A M, Reiss, C S, Mier, J W, Strominger, J L, Burakoff, S J
Human allospecific cytotoxic T lymphocyte lines were established by weekly stimulation of peripheral blood lymphocytes with allogeneic Epstein-Barr virus-transformed lymphoblastoid cell lines in the...
López De Castro, J A, Strominger, J L, Strong, D M, Orr, H T
The primary structure of two highly crossreactive human histocompatibility antigens, HLA-A28 and HLA-A2, has been determined to 96% and 90%, respectively, of the papain-solubilized molecules. Their...
Korman, A J, Auffray, C, Schamboeck, A, Strominger, J L
The amino acid sequence of the heavy chain of HLA-DR antigens has been elucidated from the analysis of a genomic clone coding for this protein. A 3.2-kilobase EcoRI fragment includes four exons...
Auffray, C, Korman, A J, Roux-Dosseto, M, Bono, R, Strominger, J L
A cDNA library has been constructed from a B cell mRNA fraction enriched for HLA-DR sequences, and cDNA clones corresponding to sequences specifically expressed in B lymphocytes have been isolated by...
Sanchez-Madrid, F, Krensky, A M, Ware, C F, Robbins, E, Strominger, J L, Burakoff, S J, ...
Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules,...
HLA antigen structural gene mutants selected with an allospecific monoclonal antibody.
Pious, D, Krangel, M S, Dixon, L L, Parham, P, Strominger, J L
The HLA-A2 antigen-specific monoclonal antibody BB7.2 and complement were used to immunoselect mutants from an ethyl methanesulfonate-mutagenized human B lymphoid cell line, T5-1. Surviving colonies...
Haynes, B F, Mann, D L, Hemler, M E, Schroer, J A, Shelhamer, J H, Eisenbarth, G S, ...
This study characterizes a monoclonal antibody (3A1), and partially characterizes the cell surface antigen and the functional peripheral blood T cell subset that it defines. The 3A1 antigen is...
DNA from the B95-8 strain of Epstein-Barr virus was cleaved into 29 different fragments by BamHI endonuclease (EC 3.1.23.6). All of the fragments except the terminal fragments have been inserted into...
Development and characterization of allospecific long-term human cytolytic T-cell lines.
Reiss, C S, Hemler, M E, Englehard, V H, Mier, J W, Strominger, J L, Burakoff, S J
Two long-term human cytolytic T lymphocyte (CTL) lines (VE and JR), whose cytolytic activity was dependent upon both irradiated JY cells (the stimulating alloantigen) and T-cell growth factor, were...
Molecular cloning of a human histocompatibility antigen cDNA fragment.
Ploegh, H L, Orr, H T, Strominger, J L
A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was...
High molecular weight antigens present on human T cells.
Judd, W, Poodry, C A, Broder, S, Friedman, S M, Chess, L, Strominger, J L
A series of eight high molecular weight (140,000-220,000) glycoproteins on human peripheral T cells were recognized by radioimmunoprecipitation with a rabbit antiserum. The pattern of antigens...
Kasai, M, Maziarz, R T, Aoki, K, Macintyre, E, Strominger, J L
A highly malignant human T-cell receptor (TCR) gamma/delta+ T-cell leukemia was shown to have a productive rearrangement of the TCR delta locus on one chromosome 14 and a novel t(8;14)(q24;q11)...
Santos-Aguado, J, Biro, P A, Fuhrmann, U, Strominger, J L, Barbosa, J A
The role of the single carbohydrate moiety present on the HLA-A2 molecule was studied by introducing several amino acid substitutions (by site-directed mutagenesis of the HLA-A2 gene) in the...
Gottesdiener, K M, Karpinski, B A, Lindsten, T, Strominger, J L, Jones, N H, Thompson, C B, ...
The human 4F2 cell surface antigen is a 120-kilodalton (kDa) disulfide-linked heterodimer which is composed of an 80- to 90-kDa glycosylated heavy chain (4F2HC) and a 35- to 40-kDa nonglycosylated...
Sequence analysis of the human major histocompatibility gene SX alpha.
Boss, J M, Mengler, R, Okada, K, Auffray, C, Strominger, J L
The DP subregion of the human major histocompatibility complex contains two closely linked gene pairs, DP alpha, DP beta and SX alpha, SX beta. The exon-intron organization and the complete DNA...
Leiden, J M, Dialynas, D P, Duby, A D, Murre, C, Seidman, J, Strominger, J L
The gene encoding the beta chain of the human T-cell receptor for antigen is composed of variable (V), diversity (D), joining (J), and constant (C) gene segments which undergo specific rearrangements...
Yang, Z, Korman, A J, Cooper, J, Pious, D, Accolla, R S, Mulligan, R C, ...
A new retrovirus vector containing the gene for hygromycin B resistance (hyg) as a selectable marker under the control of an internal simian virus 40 promoter was constructed. It was used, together...
Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens.
Ploegh, H L, Cannon, L E, Strominger, J L
Cell-free translation of poly(A)-containing RNA from the JY lymphoblastoid cell line followed by immunoprecipitation has indicated the presence of mRNAs for both the heavy and light chains of HLA-A...
Yocum, R R, Waxman, D J, Rasmussen, J R, Strominger, J L
It has been hypothesized that penicillin acts as a structural analog of the acyl-D-alanyl-D-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active...
Lancet, D, Parham, P, Strominger, J L
The three-dimensional organization of HLA antigens has been investigated by spectroscopic and immunochemical techniques. Measurement of the circular dichroism shows that in papain-solubilized HLA the...
Spies, T, Morton, C C, Nedospasov, S A, Fiers, W, Pious, D, Strominger, J L
The human major histocompatibility complex (MHC) includes the closely linked genes for the tumor necrosis factors alpha and beta. Their location is within the chromosomal segment between HLA-DR and...
To investigate the cis-acting DNA elements that are involved in regulation of class II major histocompatibility complex genes, including gamma-interferon (gamma-IFN) induction, 5' flanking DNA...
Speck, S H, Pfitzner, A, Strominger, J L
By screening a cDNA library prepared from poly(A)+ RNA isolated from an Epstein-Barr virus latently infected, growth-transformed human B-lymphoblastoid cell line, we have recovered a clone...
Structural organization of the DR subregion of the human major histocompatibility complex.
Spies, T, Sorrentino, R, Boss, J M, Okada, K, Strominger, J L
Two clusters of overlapping cosmid and lambda phage clones comprising 205 kilobases (kb) have been isolated from the DR subregion of the human major histocompatibility complex from a DR4 haplotype. A...
The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within...
Maziarz, R, Allen, H, Strominger, J L, Flavell, R, Biro, P A, Burakoff, S J
Two reciprocal interspecies hybrid class I histocompatibility genes have been constructed between genomic clones of human HLA-A2 and murine H-2Kb. The proteins encoded by these genes have been...
Collins, T, Korman, A J, Wake, C T, Boss, J M, Kappes, D J, Fiers, W, ...
Immune interferon (IFN-gamma) increases the surface expression of HLA-A,B antigens and induces the surface expression of HLA-DR antigens on vascular endothelial cells and dermal fibroblasts. Here we...
Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
The DC antigen is one of the class II major histocompatibility antigens involved in the regulation of the immune response. This molecule is a heterodimer composed of an alpha and a beta chain....
Barbosa, J A, Mentzer, S J, Minowada, G, Strominger, J L, Burakoff, S J, Biro, P A
The genes that code for the human major histocompatibility class I antigens, HLA-A2 and HLA-B7, were introduced into human, monkey, and mouse cell lines by cotransfection with suitable biochemical...
Friedman, S M, Breard, J M, Humphreys, R E, Strominger, J L, Schlossman, S F, Chess, L
A recently described technique for the polyclonal induction of plaque-forming cells from human bone-marrow derived (B) lymphocytes of peripheral blood has been used to assess the role of a human...
Reconstitution of purified detergent-soluble HLA-A and HLA-B antigens into phospholipid vesicles.
Engelhard, V H, Guild, B C, Helenius, A, Terhorst, C, Strominger, J L
Purified detergent-soluble human histocmpatibility antigens (HLA-A and HLA-B) were reconstituted into phospholipid vesicles by mixing the protein and lipid together in the presence of either...
Helenius, A, Morein, B, Fries, E, Simons, K, Robinson, P, Schirrmacher, V, ...
The proteins coded for by the HLA-A and HLA-B loci in man and the H-2K and H-2D loci in mice were identified as cell surface receptors for Semliki Forest virus. This conclusion is based on the...
Engelhard, V H, Strominger, J L, Mescher, M, Burakoff, S
Mouse cytotoxic T lymphocytes were induced by culturing primed spleen cells with cells, membranes, or detergent-solubilized and dialyzed membranes from the human lymphoblastoid cell line JY....
Pober, J S, Guild, B C, Strominger, J L
HLA-A and -B antigens are phosphorylated in transformed lymphoblastoid cells and peripheral blood lymphocytes, both incubated with 32Pi. The phosphate group is attached to HLA-A and -B heavy chain...
Guild, B C, Erikson, R L, Strominger, J L
HLA-A2 and -B7 antigens are phosphorylated by Rous sarcoma kinase (pp60v-src) in vitro. The phosphate group is attached to the heavy chains as determined by NaDodSO4/polyacrylamide gel...
White, P C, Grossberger, D, Onufer, B J, Chaplin, D D, New, M I, Dupont, B, ...
Two genes encoding steroid 21-hydroxylase [21-OHase; steroid 21-monooxygenase; steroid, hydrogen-donor: oxygen oxidoreductase (21-hydroxylating); EC 1.14.99.10], a cytochrome P-450 enzyme, have been...
Gene organization of DC and DX subregions of the human major histocompatibility complex.
Okada, K, Boss, J M, Prentice, H, Spies, T, Mengler, R, Auffray, C, ...
The DC and DX subregions of the human major histocompatibility complex (MHC) have been cloned from a cosmid library made from a human B-cell line, Priess. The DC subregion, 48 kilobases, includes the...
Sorrentino, R, Lillie, J, Strominger, J L
The monoclonal antibody 109d6, which recognizes major histocompatibility antigen MT3-like serologic determinants, has been used to characterize the molecules bearing this determinant in HLA-DR4 and...
Orr, H T, Lopez De Castro, J A, Parham, P, Ploegh, H L, Strominger, J L
The complete amino acid sequence for papain-solubilized HLA-B7 heavy chain is compared with the partial sequences of HLA-A2 and H-2Kb heavy chains. Although these molecules are highly conserved...
Dissociation and exchange of the beta 2-microglobulin subunit of HLA-A and HLA-B antigens.
Human histocompatibility antigens HLA-A, HLA-B, and HLA-C are a complex of two noncovalently associated subunits: a heavy chain glycoprotein (alpha) carrying the genetic polymorphism and an invariant...
The HLA-DR antigen, a complex of two glycoproteins of 29,000 and 34,000 daltons, can be isolated from the membranes of human B-lymphoblastoid cell lines. Extensive proteolysis releases only 5-10% of...
Turner, M. J., Strominger, J. L., Sanderson, A. R.
The removal by papain of a histocompatibility antigen, HL-A 2, from the surface of intact peripheral blood lymphocytes has been examined. Up to 80% of the antigenic determinant can be removed within...
Distribution of Ia-like molecules on the surface of normal and leukemic human cells.
Schlossman, S F, Chess, L, Humphreys, R E, Strominger, J L
Antiserum to a glycoprotein antigen complex of 23,000 and 30,000 dalton subunits (p23,30), isolated and purified from a human lymphoblastoid B cell line, was shown to be highly specific for human...
Nyormoi, O, Thorley-Lawson, D A, Elkington, J, Strominger, J L
The effects of phosphonoacetic acid on cell growth, expression of Epstein-Barr virus antigens, and virus production in human and marmoset lymphoblastoid cell lines have been studied. The drug had no...
Altered penicillin-binding components in penicillin-resistant mutants of Bacillus subtilis.
Buchanan, C E, Strominger, J L
Penicillin- (cloxacillin-) resistant mutants of Bacillus subtilis were isolated in a stepwise fashion and the five penicillin-binding components (PBCs) in each were examined to determine which of the...
Isolation of the penicillin-binding peptide from D-alanine carboxypeptidase of Bacillus subtilis.
Georgopapadakou, N, Hammarström, S, Strominger, J L
The D-alanine carboxypeptidase of B. subtilis is a membrane-bound enzyme which is inhibited by penicillins and binds them covalently. The enzyme has been labeled with [14C]- or [35S]penicillin. After...
Springer, T A, Strominger, J L
Purified, detergent-soluble HLA antigens (p44,12) are composed of a glycoprotein of molecular weight 44,000 (p44) and a peptide of molecular weight 12,000 (p12), beta2-microglobulin. Upon digestion...
Structural (shape-maintaining) role of the cell surface glycoprotein of Halobacterium salinarium.
The obligate halophile, Halobacterium salinarium, maintains a rod-shaped morphology under normal growth conditions. Lactoperoxidase(EC 1.11.1.7;donor:hydrogen-peroxide oxidoreductase)-catalyzed...
Mutants of Escherichia coli with much decreased activity of D-alanine carboxypeptidase (peptidyl-D alanine hydrolase, EC 3.4.12.11) were found among E. coli K12 extensively mutagenized with...
HLA-D associated alloantisera react with molecules similar to Ia antigens.
Giphart, M J, Kaufman, J F, Fuks, A, Albrechtsen, D, Solheim, B G, Bruning, J W, ...
Two human alloantisera specific to bone marrow-derived lymphocytes (B cells) were shown to precipitate polypeptide chains of 29,000 and 34,000 daltons from human lymphoblastoid B cell lines. These...
Further structural studies of the heavy chain of HLA antigens and its similarity to immunoglobulins.
Terhorst, C, Robb, R, Jones, C, Strominger, J L
The papain-solubilized fragment of the heavy chain of HLA-B7, which is the NH2-terminal part of the whole polypeptide chain, can be divided into three regions by mild acid and cyanogen bromide...
Immunological and chemical purity of papain-solubilized HL-A antigens.
Parham, P, Terhorst, C, Herrmann, H, Humphreys, R E, Waterfield, M D, Strominger, J L
Three preparations of purified papain-solublized HL-A antigens have been radiolabeled by reductive methylation using formaldehyde and potassium boro[3H]hydride, and their reaction with specific HL-A...
Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus.
A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as...
McCune, J M, Humphreys, R E, Yocum, R R, Strominger, J L
The amount of HL-A antigens present on the peripheral blood lymphocytes of a single human donor was increased about 11-fold after stimulation with phytohemagglutinin and 36-fold after transformation...
Hammarström, S, Strominger, J L
D-Alanine carboxypeptidase from Bacillus stearothermophilus is a membrane-bound enzyme which is inhibited by covalent interaction with penicillin G. The penicilloyl enzyme spontaneously reactivates...
Yang, Z, Accolla, R S, Pious, D, Zegers, B J, Strominger, J L
Heterokaryons were prepared and analyzed shortly after cell fusion using two mutant class-II-negative human B cell lines (RJ 2.2.5 and 6.1.6) and a cell line (TF) from a patient with a...
Wucherpfennig, K W, Catz, I, Hausmann, S, Strominger, J L, Steinman, L, Warren, K G
Myelin basic protein (MBP) may be an important autoantigen in multiple sclerosis (MS), with the MBP(82-100) region being immunodominant for T cells and autoantibodies. The structural requirements for...
Allelic variants of the human putative peptide transporter involved in antigen processing.
Colonna, M, Bresnahan, M, Bahram, S, Strominger, J L, Spies, T
Antigen processing for presentation of peptide epitopes by major histocompatibility complex (MHC) class I molecules involves genes in the MHC class II region. Among these, PSF1 and PSF2 encode...
Roux-Dosseto, M, Auffray, C, Lillie, J W, Boss, J M, Cohen, D, DeMars, R, ...
A class II antigen beta-chain cDNA clone was isolated from a human B-cell cDNA library by using as a probe the murine I-A beta gene. This cDNA clone, pHA beta, was shown to be distinct from the DC...
Okada, K, Prentice, H L, Boss, J M, Levy, D J, Kappes, D, Spies, T, ...
The SB region of the human major histocompatibility complex (MHC) has been cloned from cosmid and lambda phage libraries made from the human B-lymphoblastoid cell line Priess (DR4/4, DC4/4, SB3/4)....
Polymorphism and complexity of the human DC and murine I-A alpha chain genes.
Auffray, C, Ben-Nun, A, Roux-Dosseto, M, Germain, R N, Seidman, J G, Strominger, J L
A cDNA clone encoding the human B cell alloantigen DC alpha chain (pDCH1) has been used to analyse the structure of the human and murine major histocompatibility complexes by the DNA filter...
Structure and polymorphism of the HLA class II SB light chain genes.
Kappes, D J, Arnot, D, Okada, K, Strominger, J L
The HLA Class II region contains at least three groups of loci, DR, DC and SB, which play an important role in the immune response. The antigens encoded at these loci are heterodimers composed of an...
Valés-Gómez, M, Reyburn, H T, Erskine, R A, López-Botet, M, Strominger, J L
The lytic function of human natural killer (NK) cells is markedly influenced by recognition of class I major histocompatibility complex (MHC) molecules, a process mediated by several types of...
Gakamsky, D M, Davis, D M, Haas, E, Strominger, J L, Pecht, I
Spectral changes and a sixfold increase in the emission intensity were observed in the fluorescence of a single xanthene probe (Texas red) attached to beta2m-microglobulin (beta2m) upon assembly of...
Structural features of the cytoplasmic region of CD4 required for internalization.
Shin, J, Doyle, C, Yang, Z, Kappes, D, Strominger, J L
CD4, the T cell surface antigen, is phosphorylated and internalized when T cells are activated or treated with a phorbol ester, PMA. The actual phosphorylation sites have been identified and the role...
Peptide binding to HLA-DR1: a peptide with most residues substituted to alanine retains MHC binding.
Jardetzky, T S, Gorga, J C, Busch, R, Rothbard, J, Strominger, J L, Wiley, D C
Major histocompatibility complex (MHC) glycoproteins play an important role in the development of an effective immune response. An important MHC function is the ability to bind and present 'processed...
Sugawara, M, Scholl, T, Ponath, P D, Strominger, J L
A novel DNA sequence element termed the J element involved in the regulated expression of class II major histocompatibility complex genes was recently described. To study this element and its role in...
Rötzschke, O., Falk, K., Mack, J., Lau, J. M., Jung, G., Strominger, J. L.
Class II MHC molecules are known to exist in conformational variants. “Floppy” and “compact” forms of murine MHC molecules, for example, are discriminated by their migration behavior on...
Multiple receptors for HLA-G on human natural killer cells
Mandelboim, O., Pazmany, L., Davis, D. M., Valés-Gómez, M., Reyburn, H. T., Rybalov, B., ...
HLA-G is the putative natural killer (NK) cell inhibitory ligand expressed on the extravillous cytotrophoblast of the human placenta. Killing of the class I negative human B cell line 721.221 by NK...
Developmental plasticity of CNS microglia
Santambrogio, L., Belyanskaya, S. L., Fischer, F. R., Cipriani, B., Brosnan, C. F., Ricciardi-Castagnoli, P., ...
Microglia arise from CD45+ bone marrow precursors that colonize the fetal brain and play a key role in central nervous system inflammatory conditions. We report that parenchymal microglia are...
Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain.
Joung, I, Strominger, J L, Shin, J
A novel human cDNA encoding a cytosolic 62-kDa protein (p62) that binds to the Src homology 2 (SH2) domain of p56lck in a phosphotyrosine-independent manner has been cloned. The cDNA is composed of...
cAMP inducibility of transcriptional repressor ICER in developing and mature human T lymphocytes.
Bodor, J, Spetz, A L, Strominger, J L, Habener, J F
Stimulation of the cAMP-dependent signaling pathway exerts an inhibitory effect on the proliferation and effector functions of T cells. The ability of T cells to form high intracellular levels of...
Jardetzky, T S, Brown, J H, Gorga, J C, Stern, L J, Urban, R G, Strominger, J L, ...
The structure of the human major histocompatibility complex (MHC) class II molecule HLA-DR1 derived from the human lymphoblastoid cell line LG-2 has been determined in a complex with the...
Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution.
Tiedemann, R E, Urban, R J, Strominger, J L, Fraser, J D
Mutational studies indicate that the superantigen staphylococcal enterotoxin A (SEA) has two separate binding sites for major histocompatibility complex (MHC) class II molecules. Direct evidence is...
Park, I, Chung, J, Walsh, C T, Yun, Y, Strominger, J L, Shin, J
A previously undescribed 62-kDa protein (p62) that does not contain phosphotyrosine but, nevertheless, binds specifically to the isolated src homology 2 (SH2) domain of p56lck has been identified....
Wucherpfennig, K W, Yu, B, Bhol, K, Monos, D S, Argyris, E, Karr, R W, ...
Human T-cell-mediated autoimmune diseases are genetically linked to particular alleles of MHC class II genes. Susceptibility to pemphigus vulgaris (PV), an autoimmune disease of the skin, is linked...
Schaefer, B C, Strominger, J L, Speck, S H
The Epstein-Barr virus-encoded nuclear antigen EBNA-1 gene promoter for the restricted Epstein-Barr virus (EBV) latency program operating in group I Burkitt lymphoma (BL) cell lines was previously...
Wucherpfennig, K W, Hafler, D A, Strominger, J L
T-cell receptors (TCRs) recognize peptide bound within the relatively conserved structural framework of major histocompatibility complex (MHC) class I or class II molecules but can discriminate...
Joung, I, Kim, T, Stolz, L A, Payne, G, Winkler, D G, Walsh, C T, ...
During T-cell activation, Ser59 in the unique N-terminal region of p56lck is phosphorylated. Mutation of Ser59 to Glu59 mimics Ser59 phosphorylation, and upon CD4 crosslinking, this mutant p56lck...
Regulation of T-cell antigen receptor (TCR) alpha-chain expression by TCR beta-chain transcripts.
The TCR is an alpha beta heterodimer, a part of the multimeric structure through which physiological T-cell activation occurs. The expression of TCR alpha chain is greatly diminished in a...
A subset of HLA-B27 molecules contains peptides much longer than nonamers.
Urban, R G, Chicz, R M, Lane, W S, Strominger, J L, Rehm, A, Kenter, M J, ...
An unusual monoclonal antibody (MARB4) directed against HLA-B27 that reacts with only approximately 5-20% of the cell surface HLA-B27 was used for large-scale purification of these molecules....
Tumor necrosis factor alpha is an autocrine growth factor for normal human B cells.
Boussiotis, V A, Nadler, L M, Strominger, J L, Goldfeld, A E
Transcription of the human tumor necrosis factor alpha (TNF-alpha) gene is one of the earliest events that occurs after stimulation of B or T cells via their antigen receptors. Antibody directed at...
Hilyard, K L, Reyburn, H, Chung, S, Bell, J I, Strominger, J L
An Escherichia coli expression system has been developed to produce milligram quantities of the variable domains of a human T-cell receptor from a cytotoxic T cell that recognizes the...
Hedley, M L, Urban, R G, Strominger, J L
In vitro transcription/translation of HLA-DR1 cDNAs in the presence of microsomal membranes was used to study the association of major histocompatibility complex class II molecules with peptide and...
Functional three-domain single-chain T-cell receptors.
Chung, S, Wucherpfennig, K W, Friedman, S M, Hafler, D A, Strominger, J L
T-cell receptors (TCRs) are membrane anchored heterodimers structurally related to antibody molecules. Single-chain antibodies can be engineered by linking the two variable domains, which fold...
CD43, the major sialoglycoprotein of human leukocytes, whose expression is defective in patients with the Wiskott-Aldrich syndrome, was down-regulated by phorbol 12-myristate 13-acetate (PMA) on...
Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck.
Winkler, D G, Park, I, Kim, T, Payne, N S, Walsh, C T, Strominger, J L, ...
Ser-42 and Ser-59 in the N-terminal region have been identified as the major phorbol ester-induced phosphorylation sites of p56lck. Phosphorylation of Ser-59 results in a gel shift from 56 kDa to 61...
Guo, H C, Madden, D R, Silver, M L, Jardetzky, T S, Gorga, J C, Strominger, J L, ...
Coordinates from x-ray structures of HLA-A*6801, HLA-A*0201, and HLA-B*2705 were analyzed to examine the basis for their selectivity in peptide binding. The pocket that binds the side chain of the...
Brooks, E G, Balk, S P, Aupeix, K, Colonna, M, Strominger, J L, Groh-Spies, V
Most human T cells express the TCR alpha/beta and either CD4 or CD8 molecules (single positive, SP); however, small numbers lack CD4 and CD8. In inbred mice, alpha/beta CD4-CD8- (double negative, DN)...
Colonna, M, Borsellino, G, Falco, M, Ferrara, G B, Strominger, J L
Natural killer (NK) cells recognize alloantigens on normal cells. One of these alloantigens correlates with homozygosity for a dimorphism of HLA-C at positions 77-80, which is shared by a number of...
Allele-specific peptide ligand motifs of HLA-C molecules.
Falk, K, Rötzschke, O, Grahovac, B, Schendel, D, Stevanović, S, Gnau, V, ...
The consensus motifs of HLA-Cw3, -Cw4, -Cw6, and -Cw7 ligands were determined by pool sequencing. Together with information obtained by sequencing of some prominent individual peptides, the results...
Zinc regulates the function of two superantigens.
Fraser, J D, Urban, R G, Strominger, J L, Robinson, H
Staphylococcal enterotoxins bind with high affinity to class II major histocompatibility complex proteins and subsequently stimulate large numbers of T cells via the V beta portion of the T-cell...
Disruption of the CD4-p56lck complex is required for rapid internalization of CD4.
Sleckman, B P, Shin, J, Igras, V E, Collins, T L, Strominger, J L, Burakoff, S J
CD4 is a cell surface glycoprotein expressed by a subset of T lymphocytes and functions to enhance T-cell activation. CD4 is noncovalently associated via the cytoplasmic domain with the...
Colonna, M, Spies, T, Strominger, J L, Ciccone, E, Moretta, A, Moretta, L, ...
Human natural killer (NK) cells with the CD3- CD16+ phenotype recognize allospecificities on normal T-cell blasts. The NK-defined specificity 1 (NK-1) is recessively inherited and has been mapped to...
Goldfeld, A E, Flemington, E K, Boussiotis, V A, Theodos, C M, Titus, R G, Strominger, J L, ...
The human tumor necrosis factor alpha (TNF-alpha) gene encodes a cytokine whose activities have been implicated in many immunopathological processes, including the activation and differentiation of...
Shin, J, Dunbrack, R L, Lee, S, Strominger, J L
A mutant of CD4 (CD4.Q421stop), in which the cytoplasmic C-terminal 13 amino acids were truncated, was not expressed on the surface of HeLa cells after transfection but was retained in the...
Woisetschlaeger, M, Jin, X W, Yandava, C N, Furmanski, L A, Strominger, J L, Speck, S H
During latent Epstein-Barr virus (EBV) infection of human B lymphocytes, six viral nuclear antigen (EBNAs) are expressed from long primary transcripts by means of alternative splicing and alternative...
Expression of class II major histocompatibility complex antigens in defective B-lymphoblastoid cell lines from patients with class II antigen deficiency and from in vitro mutants generated with the...
Ono, S J, Bazil, V, Levi, B Z, Ozato, K, Strominger, J L
Transcriptional regulation of the human major histocompatibility complex class II genes requires at least two upstream elements, the X and Y boxes, located in the -50- to -150-base-pair region of all...
Ono, S J, Liou, H C, Davidon, R, Strominger, J L, Glimcher, L H
A complementary DNA encoding a member of the leucine-zipper class of proteins (human X-box-binding protein, hXBP-1) that binds to the 3' end of the conserved X box (X2) of the HLA-DRA major...
Schaefer, B C, Woisetschlaeger, M, Strominger, J L, Speck, S H
Epstein-Barr virus transformation of human B lymphocytes in vitro results in the expression of six viral nuclear antigens (EBNAs) and three viral membrane proteins. However, examination of viral gene...
Bahram, S, Arnold, D, Bresnahan, M, Strominger, J L, Spies, T
The class II region of the human major histocompatibility complex (MHC) may encode several genes controlling the processing of endogenous antigen and the presentation of peptide epitopes by MHC class...
Sugawara, M, Ponath, P D, Shin, J, Yang, Z, Strominger, J L
The cis-acting DNA sequences that control expression of the HLA-DPA and HLA-DQB promoters have been investigated in detail. A set of recombinant plasmids containing 5' promoter deletions as well as...
Positioning of a peptide in the cleft of HLA-A2 by complementing amino acid changes.
Latron, F, Moots, R, Rothbard, J B, Garrett, T P, Strominger, J L, McMichael, A
Several mutant HLA-A2 molecules have been constructed and expressed in the mutant human B-cell line C1R, which lacks HLA-A and HLA-B antigens, and examined for presentation of a previously defined...
Promoter switching in Epstein-Barr virus during the initial stages of infection of B lymphocytes.
Woisetschlaeger, M, Yandava, C N, Furmanski, L A, Strominger, J L, Speck, S H
Transcription of the genes encoding the six viral nuclear antigens present in Epstein-Barr virus latently infected lymphocytes can be initiated from one of two promoters (Cp and Wp) mapping near the...
Banerji, J, Sands, J, Strominger, J L, Spies, T
A large number of genes has been identified previously between the class I and class II gene families within the class III region of the human major histocompatibility complex. The complete sequences...
Regulation of the class II-associated invariant chain gene in normal and mutant B lymphocytes.
Doyle, C, Ford, P J, Ponath, P D, Spies, T, Strominger, J L
The invariant chain protein is intracellularly associated with class II major histocompatibility proteins. In many cases, the expression of these molecules appears to be regulated in a similar...
Maturation or differentiation of human thymocyte precursors in vitro?
Groh, V, Fabbi, M, Strominger, J L
The differentiation or maturation potential of human thymocyte precursors has been studied by using a population of CD3/TCR-, CD4-, CD8- ("triple negative") thymocytes isolated by negative selection...
Interferon gamma response region in the promoter of the human DPA gene.
Yang, Z, Sugawara, M, Ponath, P D, Wessendorf, L, Banerji, J, Li, Y, ...
The interferon gamma (IFN-gamma) response region of the human class II major histocompatibility complex gene, DPA, has been localized to a 52-base-pair (bp) DNA fragment in the proximal promotor at...
Stienekemeier, M., Falk, K., Rötzschke, O., Weishaupt, A., Schneider, C., Toyka, K. V., ...
Using a polypeptide oligomer harboring 16 repeats of the neuritogenic epitope (aa 58–73) of myelin P2 protein separated by spacers, enhancement of the immune response to the P2 protein, an...
Sedimentation characteristics of newly synthesized Epstein-Barr viral DNA in superinfected cells.
Siegel, P J, Clough, W, Strominger, J L
Replicating Epstein-Barr virus (EBV) DNA molecules isolated from superinfected Raji cells were shown to consist of 80S to 65S and 58S (mature) molecules Pulse-chase experiments showed that...
Penicillin-binding proteins in Bacillus subtilis mutants.
Kleppe, G, Yu, W, Strominger, J L
The penicillin-binding proteins (PBPs) from mutants of Bacillus subtilis were studied and related to morphology. In a previously described cloxacillin-resistant mutant of B. subtilis strain Porton,...
Macaques immunized with HLA-DR are protected from challenge with simian immunodeficiency virus.
Arthur, L O, Bess, J W, Urban, R G, Strominger, J L, Morton, W R, Mann, D L, ...
Macaques immunized with uninfected human cells have been shown to be protected from challenge with simian immunodeficiency virus (SIV) propagated in human cells. To identify the potential antigens...
Schaefer, B C, Strominger, J L, Speck, S H
The Epstein-Barr virus BamHI F promoter (Fp) was previously identified as the putative EBNA 1 gene promoter in group 1 Burkitt's lymphoma (BL) cell lines. Fp has also been shown to be activated in...
Borras, A M, Strominger, J L, Speck, S H
Induction of the Epstein-Barr virus lytic cycle is mediated through the immediate-early BZLF1 gene and the coordinately regulated BRLF1 gene. The BZLF1 gene product, Zta, transactivates its own...
Schaefer, B C, Strominger, J L, Speck, S H
In Epstein-Barr virus (EBV)-associated tumors that arise in immunocompetent individuals, the pattern of viral gene expression is very restricted compared with that of latently infected B cells in...
Identification of the active site in penicillin-binding protein 3 of Escherichia coli.
Nicholas, R A, Strominger, J L, Suzuki, H, Hirota, Y
We report the sequence of the active site tryptic peptide of penicillin-binding protein 3 from Escherichia coli. Purified penicillin-binding protein 3 was labeled with [14C]penicillin G and digested...
Penicillin-binding proteins (PBPs) 5 and 6 of Escherichia coli released the bound penicilloyl moiety at an intermediate rate relative to, e.g., Staphylococcus aureus PBPs 4 (rapid) and 1 or 2 (slow)....
Purified penicillin-binding protein 1a of Escherichia coli formed an acyl enzyme intermediate with the highly reactive synthetic substrate diacetyl-L-lysyl-D-alanyl-D-lactate at acid pH, although in...
High-molecular-weight penicillin-binding proteins from membranes of bacilli.
Waxman, D J, Lindgren, D M, Strominger, J L
Mixtures of high-molecular-weight, cephalosporin-sensitive penicillin-binding proteins (PBPs) can be purified from Bacillus subtilis membranes by cephalosporin affinity chromatography (G. Kleppe and...
Penicillin is an active-site inhibitor for four genera of bacteria.
Yocum, R R, Amanuma, H, O'Brien, T A, Waxman, D J, Strominger, J L
The hypothesis that penicillin acts as an active-site inhibitor cell wall biosynthesis was tested by a method of partial proteolytic mapping of penicillin-binding sites versus substrate-binding sites...
Purification of penicillin-binding protein 2 of Escherichia coli.
Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose...
Matsuhashi, M, Tamaki, S, Curtis, S J, Strominger, J L
The defect in D-alanine carboxypeptidase IA activity in the dacA11191 mutant of Escherichia coli was correlated with a defect in the release of penicillin G from penicillin-binding protein 5. The...
Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape.
Iwaya, M, Goldman, R, Tipper, D J, Feingold, B, Strominger, J L
Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells...
Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli.
Iwaya, M, Jones, C W, Khorana, J, Strominger, J L
Genes responsible for round morphology in mecillinam-resistant, round morphological mutants of Escherichia coli have been mapped. Three mutants, called rodX, mapped at around 14 min, and two, called...
STRUCTURE OF THE PEPTIDOGLYCAN OF BACTERIAL SPORES: OCCURRENCE OF THE LACTAM OF MURAMIC ACID*
Warth, A. D., Strominger, J. L.
Six major oligosaccharides were released from the peptidoglycan of spores of Bacillus subtilis by lysozyme treatment. They were isolated and characterized as a disaccharide, tetrasaccharide, and...
Schaefer, B C, Strominger, J L, Speck, S H
Epstein-Barr virus (EBV) is capable of adopting three distinct forms of latency: the type III latency program, in which six EBV-encoded nuclear antigens (EBNAs) are expressed, and the type I and type...
Schaefer, B C, Paulson, E, Strominger, J L, Speck, S H
The Epstein-Barr virus (EBV) EBNA1 gene promoter active in the type I program of restricted viral latency was recently identified and shown to reside in the viral BamHI Q fragment. This promoter, Qp,...
Mapping of a new genetic locus responsible for ampicillin resistance in Escherichia coli.
Buchanan, C E, Strominger, J L
The ampicillin resistance locus of three different ampicillin-resistant, temperature-sensitive Escherichia coli mutants was mapped between proC and purE and does not correspond to any of the known...
Electron microscope studies of conditional spore cortexless mutants of Bacillus sphaericus.
Imae, Y, Strominger, M B, Strominger, J L
Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid...
Relationship between cortex content and properties of Bacillus sphaericus spores.
The muramic lactam content of spores of Bacillus sphaericus mutants defective in meso-diaminopimelic acid synthesis increases almost linearly with an increase of meso-diaminopimelic acid...
Cortex content of asporogenous mutants of Bacillus subtilis.
A method for the measurement of muramic lactam, which is specifically located in the cortical peptidoglycan of bacterial spores, was developed as a quantitative assay method for spore cortex content....
Protein and Carbohydrate Composition of the Cell Envelope of Halobacterium salinarium1
Mescher, M. F., Strominger, J. L., Watson, S. W.
The isolated cell envelope of Halobacterium salinarium strain 1 contained 15 to 20 proteins that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. All but...
Skare, J, Farley, J, Strominger, J L, Fresen, K O, Cho, M S, Zur Hausen, H
Eight independent recombinant Epstein-Barr virus genomes, each of which was a transforming strain, were made by superinfecting cell lines containing Epstein-Barr virus DNA (Raji or B95-8 strain) with...
Pfitzner, A J, Tsai, E C, Strominger, J L, Speck, S H
The Epstein-Barr virus (EBV) mutant P3HR1 is incapable of immortalizing B lymphocytes because of a 6.8-kilobase deletion in the BamHI W, Y, and H regions of the viral genome (M. Rabson, L....
Pfitzner, A J, Strominger, J L, Speck, S H
A 1.95-kilobase cDNA clone was isolated by screening a size-selected lambda gt10 cDNA library prepared from an Epstein-Barr virus-transformed B-cell line, IB4, with the Epstein-Barr virus BamHI M...
The B95-8 isolate of Epstein-Barr virus arose from an isolate with a standard genome.
Skare, J, Edson, C, Farley, J, Strominger, J L
Blot hybridization studies revealed that the deletion which characterizes the DNA from the B95-8 strain of Epstein-Barr virus was not present in the virus from which the B95-8 strain was derived...
Latron, F, Jotterand-Bellomo, M, Maffei, A, Scarpellino, L, Bernard, M, Strominger, J L, ...
Constitutive expression of major histocompatibility complex class II genes is acquired very early in B-cell ontogeny and is maintained up to the B-cell blast stage. Terminal differentiation in plasma...
Austin, P J, Flemington, E, Yandava, C N, Strominger, J L, Speck, S H
Several cDNA clones containing the Epstein-Barr virus BamHI fragment H rightward open reading frame 1 (BHRF1) have been recovered from the tightly latent lymphoblastoid cell line IB4. These clones...
A 95-base-pair immediate upstream sequence of the human class II major histocompatibility complex DQB gene containing the conserved X and Y elements showed enhancer activity in a transient expression...
Genomic organization of the human T-cell antigen-receptor alpha/delta locus.
Satyanarayana, K, Hata, S, Devlin, P, Roncarolo, M G, De Vries, J E, Spits, H, ...
Two clusters of overlapping cosmid clones comprising about 100 kilobases (kb) at the human T-cell antigen-receptor alpha/delta locus were isolated from a genomic library. The structure of the...
McMichael, A J, Gotch, F M, Santos-Aguado, J, Strominger, J L
Cytotoxic T lymphocytes (CTL) specific for influenza A virus were prepared from 15 donors. Those with HLA-A2 recognized autologous or HLA-A2-matched B-lymphoblastoid cells in the presence of...
Dietzler, D. N., Strominger, J. L.
The enzyme which catalyzes the transfer of glucosyl residues from adenosine diphospho(ADP)-glucose to glycogen has been partially purified from extracts of Pasteurella pseudotuberculosis. In contrast...
Identification of murine complement receptor type 2.
Fingeroth, J D, Benedict, M A, Levy, D N, Strominger, J L
A rabbit antiserum reactive with the human complement component C3d/Epstein-Barr virus receptor (complement receptor type 2, CR2) immunoprecipitates a Mr 155,000 murine B-cell surface antigen. The...
Gorga, J C, Dong, A, Manning, M C, Woody, R W, Caughey, W S, Strominger, J L
We have examined the secondary structures of human class I and class II histocompatibility antigens in solution by Fourier transform infrared spectroscopy and circular dichroism in order to compare...
HLA class I antigens present in the human leukemia T-cell line HPB-ALL are shown to be endocytosed in the absence of specific antibodies. In 1 hr, approximately 10% of class I molecules initially...
Groh, V, Fabbi, M, Hochstenbach, F, Maziarz, R T, Strominger, J L
Double-negative (CD4-CD8-) T-cell receptor alpha- and beta-chain (TCR alpha beta)-expressing lymphocytes have been identified in human skin. Two skin-derived lymphocyte cell lines (II-1 and II-2)...
Mutually exclusive use of viral promoters in Epstein-Barr virus latently infected lymphocytes.
Woisetschlaeger, M, Strominger, J L, Speck, S H
Of the eight viral antigens known to be expressed during Epstein-Barr virus latency, six are transcribed from a major rightward transcriptional unit, which gives rise to mRNAs containing common 5'...
Alloreactivity studied with mutants of HLA-A2.
Santos-Aguado, J, Crimmins, M A, Mentzer, S J, Burakoff, S J, Strominger, J L
Based on the crystal structure of HLA-A2.1 and the recognition of a panel of mutant HLA-A2.1 molecules by a large number of alloreactive cytotoxic T lymphocyte clones, a model to explain...
Spies, T, Bresnahan, M, Strominger, J L
A 600-kilobase (kb) DNA segment from the human major histocompatibility complex (MHC) class III region was isolated by extension of a previous 435-kb chromosome walk. The contiguous series of cloned...
Quackenbush, E, Clabby, M, Gottesdiener, K M, Barbosa, J, Jones, N H, Strominger, J L, ...
Complementary DNA (cDNA) clones encoding the heavy chain of the heterodimeric human membrane glycoprotein 4F2 have been isolated by immunoscreening of a lambda gt11 expression library. The identity...
Linkage map of the human major histocompatibility complex including the tumor necrosis factor genes.
Carroll, M C, Katzman, P, Alicot, E M, Koller, B H, Geraghty, D E, Orr, H T, ...
The tumor necrosis factor (TNF) alpha and beta gene pair has been linked in the human major histocompatibility complex to HLA-B, HLA-C, and, tentatively, HLA-E and HLA-A on one side and to the class...
Huang, H J, Jones, N H, Strominger, J L, Herzenberg, L A
We report the isolation of cDNA clones of the mouse lymphocyte differentiation antigen Ly-1. One of these cDNA clones was confirmed to be full-length by DNA sequencing and by expression of Ly-1 by L...
Expression of human class II major histocompatibility complex antigens using retrovirus vectors.
Korman, A J, Frantz, J D, Strominger, J L, Mulligan, R C
Retrovirus vectors [direct orientation (DO) vectors] that permit the simultaneous expression of an inserted protein-coding sequence and a dominant-acting selectable marker have been constructed. In...
Takada, Y, Strominger, J L, Hemler, M E
The very late antigen (VLA) protein family contains at least five related heterodimers, including a fibronectin receptor structure, and probably other cell substrate adhesion receptors. These...
Krangel, M S, Bierer, B E, Devlin, P, Clabby, M, Strominger, J L, McLean, J, ...
The T-cell receptor (TCR) gamma gene product occurs in association with T3 (CD3) polypeptides on the surface of human T lymphocytes. TCR gamma lymphocytes express arrays of T3 polypeptides distinct...
Miwa, K, Doyle, C, Strominger, J L
All class II major histocompatibility complex genes contain two highly conserved sequences, termed X and Y, within the promoter regions(s), which may have a role in regulation of expression. To study...
Biochemical Studies of the Smooth-Rough Mutation in Salmonella minnesota
Lüderitz, O., Risse, H. J., Schulte-Holthausen, H., Strominger, J. L., Sutherland, I. W., Westphal, O.
Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the...
A method is described for scanning relatively large fragments of DNA for sequences which bind nuclear factors. This method was used to identify an octamer (ATTTGTAT) in the DQ beta gene upstream...
Irigoyen, O H, Rizzolo, P V, Thomas, Y, Hemler, M E, Shen, H H, Friedman, S M, ...
A monoclonal antibody, PVR-11, was obtained after hybridization of X63Ag8.653 murine myeloma cells with spleen cells from a mouse immunized with human lymphocytes. It recognizes a 175,000- to...
Human T cell surface antigens bearing a structural relationship to HLA antigens.
Cotner, T, Mashimo, H, Kung, P C, Goldstein, G, Strominger, J L
Three cell surface antigens that are structurally related to the human major histocompatibility antigens (called HLA antigens) have been characterized from the leukemic T cell line MOLT-4. One...
Mulder, A, Alexander, S, Engelfriet, C P, Von Dem Borne, A E, Strominger, J L
The human promyelocytic leukemia cell line HL-60 is reactive with an antiserum raised against normal human granulocytes (AGS). Immunoprecipitation with AGS on [35S]methionine-labeled HL-60 cell...
Collins, T, Lapierre, L A, Fiers, W, Strominger, J L, Pober, J S
Recombinant human tumor necrosis factor (TNF), purified to greater than 99% homogeneity, increases surface expression of class I major histocompatibility complex (MHC) antigens to a maximum of 9-fold...
Dialynas, D P, Murre, C, Quertermous, T, Boss, J M, Leiden, J M, Seidman, J G, ...
Complementary DNA (cDNA) encoding a human T-cell gamma chain has been cloned and sequenced. At the junction of the variable and joining regions, there is an apparent deletion of two nucleotides in...
Generation of diversity of the beta chain of the human T-lymphocyte receptor for antigen.
Human T-cell receptor beta-chain (Ti beta) genes are formed by the rearrangement of variable (V), diversity (D), and joining (J) gene segments. A comparison of the nucleotide and deduced amino acid...
Schamboeck, A, Korman, A J, Kamb, A, Strominger, J L
A total of 5724 base pairs of a recombinant phage DNA containing a human HLA-DR heavy chain gene including flanking regions has been analyzed. The regions corresponding to all the exons have been...
Terhorst, C, Parham, P, Mann, D L, Strominger, J L
Amino-acid and carbohydrate compositions in addition to limited NH2-terminal sequence data of four preparations of HLA antigens, including products of both of the major histocompatibility loci (HLA-A...
Morton, C C, Kirsch, I R, Nance, W E, Evans, G A, Korman, A J, Strominger, J L
We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization. Our data indicate that a cloned...
Identification and mapping of polypeptides encoded by the P3HR-1 strain of Epstein-Barr virus.
Cohen, L K, Speck, S H, Roberts, B E, Strominger, J L
The Epstein-Barr virus (EBV)-specified polypeptides induced upon viral replication in the P3HR-1 cell line have been examined by immunoprecipitation with a high-titer human anti-EBV serum....
Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells.
Hemler, M E, Brenner, M B, McLean, J M, Strominger, J L
Antigen-specific, interleukin 2 (IL-2)-dependent human T-cell lines and clones were utilized to study the relationship between IL-2 receptor expression and antigenic stimulation. T cells that had not...
Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2.
Fingeroth, J D, Weis, J J, Tedder, T F, Strominger, J L, Biro, P A, Fearon, D T
Identity of the Epstein-Barr virus (EBV) receptor with the complement receptor type 2 (CR2) was established in three sets of experiments using the monoclonal antibodies, HB-5 and anti-B2, which...
Korman, A J, Knudsen, P J, Kaufman, J F, Strominger, J L
A monoclonal antibody (HC 2.1) directed against the separated heavy chain of HLA-DR has been prepared. By binding HC 2.1 to polysomes from human B lymphoblastoid cells followed by the use of a...
Long-term human cytolytic T-cell lines allospecific for HLA-DR6 antigen are OKT4+.
Krensky, A M, Reiss, C S, Mier, J W, Strominger, J L, Burakoff, S J
Human allospecific cytotoxic T lymphocyte lines were established by weekly stimulation of peripheral blood lymphocytes with allogeneic Epstein-Barr virus-transformed lymphoblastoid cell lines in the...
López De Castro, J A, Strominger, J L, Strong, D M, Orr, H T
The primary structure of two highly crossreactive human histocompatibility antigens, HLA-A28 and HLA-A2, has been determined to 96% and 90%, respectively, of the papain-solubilized molecules. Their...
Korman, A J, Auffray, C, Schamboeck, A, Strominger, J L
The amino acid sequence of the heavy chain of HLA-DR antigens has been elucidated from the analysis of a genomic clone coding for this protein. A 3.2-kilobase EcoRI fragment includes four exons...
Auffray, C, Korman, A J, Roux-Dosseto, M, Bono, R, Strominger, J L
A cDNA library has been constructed from a B cell mRNA fraction enriched for HLA-DR sequences, and cDNA clones corresponding to sequences specifically expressed in B lymphocytes have been isolated by...
Sanchez-Madrid, F, Krensky, A M, Ware, C F, Robbins, E, Strominger, J L, Burakoff, S J, ...
Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules,...
HLA antigen structural gene mutants selected with an allospecific monoclonal antibody.
Pious, D, Krangel, M S, Dixon, L L, Parham, P, Strominger, J L
The HLA-A2 antigen-specific monoclonal antibody BB7.2 and complement were used to immunoselect mutants from an ethyl methanesulfonate-mutagenized human B lymphoid cell line, T5-1. Surviving colonies...
Haynes, B F, Mann, D L, Hemler, M E, Schroer, J A, Shelhamer, J H, Eisenbarth, G S, ...
This study characterizes a monoclonal antibody (3A1), and partially characterizes the cell surface antigen and the functional peripheral blood T cell subset that it defines. The 3A1 antigen is...
DNA from the B95-8 strain of Epstein-Barr virus was cleaved into 29 different fragments by BamHI endonuclease (EC 3.1.23.6). All of the fragments except the terminal fragments have been inserted into...
Development and characterization of allospecific long-term human cytolytic T-cell lines.
Reiss, C S, Hemler, M E, Englehard, V H, Mier, J W, Strominger, J L, Burakoff, S J
Two long-term human cytolytic T lymphocyte (CTL) lines (VE and JR), whose cytolytic activity was dependent upon both irradiated JY cells (the stimulating alloantigen) and T-cell growth factor, were...
Molecular cloning of a human histocompatibility antigen cDNA fragment.
Ploegh, H L, Orr, H T, Strominger, J L
A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was...
High molecular weight antigens present on human T cells.
Judd, W, Poodry, C A, Broder, S, Friedman, S M, Chess, L, Strominger, J L
A series of eight high molecular weight (140,000-220,000) glycoproteins on human peripheral T cells were recognized by radioimmunoprecipitation with a rabbit antiserum. The pattern of antigens...
Sugawara, M, Scholl, T, Ponath, P D, Strominger, J L
A novel DNA sequence element termed the J element involved in the regulated expression of class II major histocompatibility complex genes was recently described. To study this element and its role in...
Kasai, M, Maziarz, R T, Aoki, K, Macintyre, E, Strominger, J L
A highly malignant human T-cell receptor (TCR) gamma/delta+ T-cell leukemia was shown to have a productive rearrangement of the TCR delta locus on one chromosome 14 and a novel t(8;14)(q24;q11)...
Santos-Aguado, J, Biro, P A, Fuhrmann, U, Strominger, J L, Barbosa, J A
The role of the single carbohydrate moiety present on the HLA-A2 molecule was studied by introducing several amino acid substitutions (by site-directed mutagenesis of the HLA-A2 gene) in the...
Gottesdiener, K M, Karpinski, B A, Lindsten, T, Strominger, J L, Jones, N H, Thompson, C B, ...
The human 4F2 cell surface antigen is a 120-kilodalton (kDa) disulfide-linked heterodimer which is composed of an 80- to 90-kDa glycosylated heavy chain (4F2HC) and a 35- to 40-kDa nonglycosylated...
Sequence analysis of the human major histocompatibility gene SX alpha.
Boss, J M, Mengler, R, Okada, K, Auffray, C, Strominger, J L
The DP subregion of the human major histocompatibility complex contains two closely linked gene pairs, DP alpha, DP beta and SX alpha, SX beta. The exon-intron organization and the complete DNA...
Leiden, J M, Dialynas, D P, Duby, A D, Murre, C, Seidman, J, Strominger, J L
The gene encoding the beta chain of the human T-cell receptor for antigen is composed of variable (V), diversity (D), joining (J), and constant (C) gene segments which undergo specific rearrangements...
Yang, Z, Korman, A J, Cooper, J, Pious, D, Accolla, R S, Mulligan, R C, ...
A new retrovirus vector containing the gene for hygromycin B resistance (hyg) as a selectable marker under the control of an internal simian virus 40 promoter was constructed. It was used, together...
Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens.
Ploegh, H L, Cannon, L E, Strominger, J L
Cell-free translation of poly(A)-containing RNA from the JY lymphoblastoid cell line followed by immunoprecipitation has indicated the presence of mRNAs for both the heavy and light chains of HLA-A...
Yocum, R R, Waxman, D J, Rasmussen, J R, Strominger, J L
It has been hypothesized that penicillin acts as a structural analog of the acyl-D-alanyl-D-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active...
Lancet, D, Parham, P, Strominger, J L
The three-dimensional organization of HLA antigens has been investigated by spectroscopic and immunochemical techniques. Measurement of the circular dichroism shows that in papain-solubilized HLA the...
Spies, T, Morton, C C, Nedospasov, S A, Fiers, W, Pious, D, Strominger, J L
The human major histocompatibility complex (MHC) includes the closely linked genes for the tumor necrosis factors alpha and beta. Their location is within the chromosomal segment between HLA-DR and...
To investigate the cis-acting DNA elements that are involved in regulation of class II major histocompatibility complex genes, including gamma-interferon (gamma-IFN) induction, 5' flanking DNA...
Speck, S H, Pfitzner, A, Strominger, J L
By screening a cDNA library prepared from poly(A)+ RNA isolated from an Epstein-Barr virus latently infected, growth-transformed human B-lymphoblastoid cell line, we have recovered a clone...
Structural organization of the DR subregion of the human major histocompatibility complex.
Spies, T, Sorrentino, R, Boss, J M, Okada, K, Strominger, J L
Two clusters of overlapping cosmid and lambda phage clones comprising 205 kilobases (kb) have been isolated from the DR subregion of the human major histocompatibility complex from a DR4 haplotype. A...
The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within...
Maziarz, R, Allen, H, Strominger, J L, Flavell, R, Biro, P A, Burakoff, S J
Two reciprocal interspecies hybrid class I histocompatibility genes have been constructed between genomic clones of human HLA-A2 and murine H-2Kb. The proteins encoded by these genes have been...
Collins, T, Korman, A J, Wake, C T, Boss, J M, Kappes, D J, Fiers, W, ...
Immune interferon (IFN-gamma) increases the surface expression of HLA-A,B antigens and induces the surface expression of HLA-DR antigens on vascular endothelial cells and dermal fibroblasts. Here we...
Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
The DC antigen is one of the class II major histocompatibility antigens involved in the regulation of the immune response. This molecule is a heterodimer composed of an alpha and a beta chain....
Barbosa, J A, Mentzer, S J, Minowada, G, Strominger, J L, Burakoff, S J, Biro, P A
The genes that code for the human major histocompatibility class I antigens, HLA-A2 and HLA-B7, were introduced into human, monkey, and mouse cell lines by cotransfection with suitable biochemical...
Friedman, S M, Breard, J M, Humphreys, R E, Strominger, J L, Schlossman, S F, Chess, L
A recently described technique for the polyclonal induction of plaque-forming cells from human bone-marrow derived (B) lymphocytes of peripheral blood has been used to assess the role of a human...
Reconstitution of purified detergent-soluble HLA-A and HLA-B antigens into phospholipid vesicles.
Engelhard, V H, Guild, B C, Helenius, A, Terhorst, C, Strominger, J L
Purified detergent-soluble human histocmpatibility antigens (HLA-A and HLA-B) were reconstituted into phospholipid vesicles by mixing the protein and lipid together in the presence of either...
Helenius, A, Morein, B, Fries, E, Simons, K, Robinson, P, Schirrmacher, V, ...
The proteins coded for by the HLA-A and HLA-B loci in man and the H-2K and H-2D loci in mice were identified as cell surface receptors for Semliki Forest virus. This conclusion is based on the...
Engelhard, V H, Strominger, J L, Mescher, M, Burakoff, S
Mouse cytotoxic T lymphocytes were induced by culturing primed spleen cells with cells, membranes, or detergent-solubilized and dialyzed membranes from the human lymphoblastoid cell line JY....
Pober, J S, Guild, B C, Strominger, J L
HLA-A and -B antigens are phosphorylated in transformed lymphoblastoid cells and peripheral blood lymphocytes, both incubated with 32Pi. The phosphate group is attached to HLA-A and -B heavy chain...
Guild, B C, Erikson, R L, Strominger, J L
HLA-A2 and -B7 antigens are phosphorylated by Rous sarcoma kinase (pp60v-src) in vitro. The phosphate group is attached to the heavy chains as determined by NaDodSO4/polyacrylamide gel...
White, P C, Grossberger, D, Onufer, B J, Chaplin, D D, New, M I, Dupont, B, ...
Two genes encoding steroid 21-hydroxylase [21-OHase; steroid 21-monooxygenase; steroid, hydrogen-donor: oxygen oxidoreductase (21-hydroxylating); EC 1.14.99.10], a cytochrome P-450 enzyme, have been...
Gene organization of DC and DX subregions of the human major histocompatibility complex.
Okada, K, Boss, J M, Prentice, H, Spies, T, Mengler, R, Auffray, C, ...
The DC and DX subregions of the human major histocompatibility complex (MHC) have been cloned from a cosmid library made from a human B-cell line, Priess. The DC subregion, 48 kilobases, includes the...
Sorrentino, R, Lillie, J, Strominger, J L
The monoclonal antibody 109d6, which recognizes major histocompatibility antigen MT3-like serologic determinants, has been used to characterize the molecules bearing this determinant in HLA-DR4 and...
Orr, H T, Lopez De Castro, J A, Parham, P, Ploegh, H L, Strominger, J L
The complete amino acid sequence for papain-solubilized HLA-B7 heavy chain is compared with the partial sequences of HLA-A2 and H-2Kb heavy chains. Although these molecules are highly conserved...
Dissociation and exchange of the beta 2-microglobulin subunit of HLA-A and HLA-B antigens.
Human histocompatibility antigens HLA-A, HLA-B, and HLA-C are a complex of two noncovalently associated subunits: a heavy chain glycoprotein (alpha) carrying the genetic polymorphism and an invariant...
The HLA-DR antigen, a complex of two glycoproteins of 29,000 and 34,000 daltons, can be isolated from the membranes of human B-lymphoblastoid cell lines. Extensive proteolysis releases only 5-10% of...
Turner, M. J., Strominger, J. L., Sanderson, A. R.
The removal by papain of a histocompatibility antigen, HL-A 2, from the surface of intact peripheral blood lymphocytes has been examined. Up to 80% of the antigenic determinant can be removed within...
Distribution of Ia-like molecules on the surface of normal and leukemic human cells.
Schlossman, S F, Chess, L, Humphreys, R E, Strominger, J L
Antiserum to a glycoprotein antigen complex of 23,000 and 30,000 dalton subunits (p23,30), isolated and purified from a human lymphoblastoid B cell line, was shown to be highly specific for human...
Nyormoi, O, Thorley-Lawson, D A, Elkington, J, Strominger, J L
The effects of phosphonoacetic acid on cell growth, expression of Epstein-Barr virus antigens, and virus production in human and marmoset lymphoblastoid cell lines have been studied. The drug had no...
Altered penicillin-binding components in penicillin-resistant mutants of Bacillus subtilis.
Buchanan, C E, Strominger, J L
Penicillin- (cloxacillin-) resistant mutants of Bacillus subtilis were isolated in a stepwise fashion and the five penicillin-binding components (PBCs) in each were examined to determine which of the...
Isolation of the penicillin-binding peptide from D-alanine carboxypeptidase of Bacillus subtilis.
Georgopapadakou, N, Hammarström, S, Strominger, J L
The D-alanine carboxypeptidase of B. subtilis is a membrane-bound enzyme which is inhibited by penicillins and binds them covalently. The enzyme has been labeled with [14C]- or [35S]penicillin. After...
Springer, T A, Strominger, J L
Purified, detergent-soluble HLA antigens (p44,12) are composed of a glycoprotein of molecular weight 44,000 (p44) and a peptide of molecular weight 12,000 (p12), beta2-microglobulin. Upon digestion...
Structural (shape-maintaining) role of the cell surface glycoprotein of Halobacterium salinarium.
The obligate halophile, Halobacterium salinarium, maintains a rod-shaped morphology under normal growth conditions. Lactoperoxidase(EC 1.11.1.7;donor:hydrogen-peroxide oxidoreductase)-catalyzed...
Mutants of Escherichia coli with much decreased activity of D-alanine carboxypeptidase (peptidyl-D alanine hydrolase, EC 3.4.12.11) were found among E. coli K12 extensively mutagenized with...
HLA-D associated alloantisera react with molecules similar to Ia antigens.
Giphart, M J, Kaufman, J F, Fuks, A, Albrechtsen, D, Solheim, B G, Bruning, J W, ...
Two human alloantisera specific to bone marrow-derived lymphocytes (B cells) were shown to precipitate polypeptide chains of 29,000 and 34,000 daltons from human lymphoblastoid B cell lines. These...
Further structural studies of the heavy chain of HLA antigens and its similarity to immunoglobulins.
Terhorst, C, Robb, R, Jones, C, Strominger, J L
The papain-solubilized fragment of the heavy chain of HLA-B7, which is the NH2-terminal part of the whole polypeptide chain, can be divided into three regions by mild acid and cyanogen bromide...
Immunological and chemical purity of papain-solubilized HL-A antigens.
Parham, P, Terhorst, C, Herrmann, H, Humphreys, R E, Waterfield, M D, Strominger, J L
Three preparations of purified papain-solublized HL-A antigens have been radiolabeled by reductive methylation using formaldehyde and potassium boro[3H]hydride, and their reaction with specific HL-A...
Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus.
A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as...
McCune, J M, Humphreys, R E, Yocum, R R, Strominger, J L
The amount of HL-A antigens present on the peripheral blood lymphocytes of a single human donor was increased about 11-fold after stimulation with phytohemagglutinin and 36-fold after transformation...
Hammarström, S, Strominger, J L
D-Alanine carboxypeptidase from Bacillus stearothermophilus is a membrane-bound enzyme which is inhibited by covalent interaction with penicillin G. The penicilloyl enzyme spontaneously reactivates...
Yang, Z, Accolla, R S, Pious, D, Zegers, B J, Strominger, J L
Heterokaryons were prepared and analyzed shortly after cell fusion using two mutant class-II-negative human B cell lines (RJ 2.2.5 and 6.1.6) and a cell line (TF) from a patient with a...
Wucherpfennig, K W, Catz, I, Hausmann, S, Strominger, J L, Steinman, L, Warren, K G
Myelin basic protein (MBP) may be an important autoantigen in multiple sclerosis (MS), with the MBP(82-100) region being immunodominant for T cells and autoantibodies. The structural requirements for...
Allelic variants of the human putative peptide transporter involved in antigen processing.
Colonna, M, Bresnahan, M, Bahram, S, Strominger, J L, Spies, T
Antigen processing for presentation of peptide epitopes by major histocompatibility complex (MHC) class I molecules involves genes in the MHC class II region. Among these, PSF1 and PSF2 encode...
Roux-Dosseto, M, Auffray, C, Lillie, J W, Boss, J M, Cohen, D, DeMars, R, ...
A class II antigen beta-chain cDNA clone was isolated from a human B-cell cDNA library by using as a probe the murine I-A beta gene. This cDNA clone, pHA beta, was shown to be distinct from the DC...
Structural features of the cytoplasmic region of CD4 required for internalization.
Shin, J, Doyle, C, Yang, Z, Kappes, D, Strominger, J L
CD4, the T cell surface antigen, is phosphorylated and internalized when T cells are activated or treated with a phorbol ester, PMA. The actual phosphorylation sites have been identified and the role...
Peptide binding to HLA-DR1: a peptide with most residues substituted to alanine retains MHC binding.
Jardetzky, T S, Gorga, J C, Busch, R, Rothbard, J, Strominger, J L, Wiley, D C
Major histocompatibility complex (MHC) glycoproteins play an important role in the development of an effective immune response. An important MHC function is the ability to bind and present 'processed...
Okada, K, Prentice, H L, Boss, J M, Levy, D J, Kappes, D, Spies, T, ...
The SB region of the human major histocompatibility complex (MHC) has been cloned from cosmid and lambda phage libraries made from the human B-lymphoblastoid cell line Priess (DR4/4, DC4/4, SB3/4)....
Polymorphism and complexity of the human DC and murine I-A alpha chain genes.
Auffray, C, Ben-Nun, A, Roux-Dosseto, M, Germain, R N, Seidman, J G, Strominger, J L
A cDNA clone encoding the human B cell alloantigen DC alpha chain (pDCH1) has been used to analyse the structure of the human and murine major histocompatibility complexes by the DNA filter...
Structure and polymorphism of the HLA class II SB light chain genes.
Kappes, D J, Arnot, D, Okada, K, Strominger, J L
The HLA Class II region contains at least three groups of loci, DR, DC and SB, which play an important role in the immune response. The antigens encoded at these loci are heterodimers composed of an...
Valés-Gómez, M, Reyburn, H T, Erskine, R A, López-Botet, M, Strominger, J L
The lytic function of human natural killer (NK) cells is markedly influenced by recognition of class I major histocompatibility complex (MHC) molecules, a process mediated by several types of...
Gakamsky, D M, Davis, D M, Haas, E, Strominger, J L, Pecht, I
Spectral changes and a sixfold increase in the emission intensity were observed in the fluorescence of a single xanthene probe (Texas red) attached to beta2m-microglobulin (beta2m) upon assembly of...