J. M. Requena

Details der Publikationsliste

Zeitraum

1989 - 2003

Anzahl

23

Co-Autoren

A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei.

Jimenez-Ruiz, A, Requena, J M, Lopez, M C, Alonso, C

Recent studies have demonstrated that Z-DNA exists in vivo in Escherichia coli as well as in Drosophila and mammalian cells. In the present paper, we show the existence in vivo of Z-DNA epitopes in...

Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins.

Soto, M, Requena, J M, Quijada, L, Alonso, C

The Leishmania P2 proteins have been analyzed as potential tools for the immunodiagnosis of human mucocutaneous and visceral leishmaniasis. Two recombinant Leishmania infantum proteins, rLIP2a and...

Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes.

Jimenez-Ruiz, A, Requena, J M, Lancillotti, F, Morales, G, Lopez, M C, Alonso, C

We describe the selection of a group of plasmids with potential to form Z-DNA, from libraries of Drosophila hydei nuclear DNA using anti Z-DNA monoclonal (22) or polyclonal (10c) antibodies. The...

A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi.

Requena, J M, López, M C, Jimenez-Ruiz, A, De La Torre, J C, Alonso, C

We describe the isolation and characterization of the T. cruzi hsp 70 DNA coding region which was found to be formed by multigene copies organized in a tandem array in a head-to-tail manner. The...

Organization, transcription and regulation of the Leishmania infantum histone H3 genes.

Soto, M, Requena, J M, Quijada, L, Alonso, C

The genomic organization and transcription of the genes encoding the histone H3 of the protozoan parasite Leishmania infantum have been studied. It was found that there are multiple copies of the...

Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control.

Soto, M, Quijada, L, Alonso, C, Requena, J M

We have analysed the regulation of histone synthesis in Leishmania infantum following inhibition of DNA replication. Run-on experiments indicated that transcription rates of the genes coding for the...

A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei.

Jimenez-Ruiz, A, Requena, J M, Lopez, M C, Alonso, C

Recent studies have demonstrated that Z-DNA exists in vivo in Escherichia coli as well as in Drosophila and mammalian cells. In the present paper, we show the existence in vivo of Z-DNA epitopes in...

Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins.

Soto, M, Requena, J M, Quijada, L, Alonso, C

The Leishmania P2 proteins have been analyzed as potential tools for the immunodiagnosis of human mucocutaneous and visceral leishmaniasis. Two recombinant Leishmania infantum proteins, rLIP2a and...

Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes.

Jimenez-Ruiz, A, Requena, J M, Lancillotti, F, Morales, G, Lopez, M C, Alonso, C

We describe the selection of a group of plasmids with potential to form Z-DNA, from libraries of Drosophila hydei nuclear DNA using anti Z-DNA monoclonal (22) or polyclonal (10c) antibodies. The...

A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi.

Requena, J M, López, M C, Jimenez-Ruiz, A, De La Torre, J C, Alonso, C

We describe the isolation and characterization of the T. cruzi hsp 70 DNA coding region which was found to be formed by multigene copies organized in a tandem array in a head-to-tail manner. The...

Organization, transcription and regulation of the Leishmania infantum histone H3 genes.

Soto, M, Requena, J M, Quijada, L, Alonso, C

The genomic organization and transcription of the genes encoding the histone H3 of the protozoan parasite Leishmania infantum have been studied. It was found that there are multiple copies of the...

Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control.

Soto, M, Quijada, L, Alonso, C, Requena, J M

We have analysed the regulation of histone synthesis in Leishmania infantum following inhibition of DNA replication. Run-on experiments indicated that transcription rates of the genes coding for the...

During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins.

Soto, M, Requena, J M, Quijada, L, Angel, S O, Gomez, L C, Guzman, F, ...

In this work we show that in the sera from dogs naturally infected with the protozoan parasite Leishmania infantum there are antibodies that react specifically against the parasite acidic ribosomal...

Antigenicity of the Leishmania infantum histones H2B and H4 during canine viscerocutaneous leishmaniasis

Soto, M, Requena, J M, Quijada, L, Perez, M J, Nieto, C G, Guzman, F, ...

In this study we show that sera from dogs naturally infected with Leishmania infantum contain antibodies that specifically react against the parasite H2B and H4 histones. The Leishmania H2B and the...

The Leishmania infantum acidic ribosomal protein LiP2a induces a prominent humoral response in vivo and stimulates cell proliferation in vitro and interferon-gamma (IFN-γ) production by murine splenocytes

Soto, M, Alonso, C, Requena, J M

The acidic ribosomal proteins of the protozoan parasite Leishmania infantum have been described as prominent antigens during both human and canine visceral leishmaniasis. In this study we present...

Characterization of the antigenic determinants of the Leishmania infantum histone H3 recognized by antibodies elicited during canine visceral leishmaniasis

SOTO, M, REQUENA, J M, QUIJADA, L, GOMEZ, L C, GUZMAN, F, PATARROYO, M E, ...

In the present study we show that sera from dogs naturally infected with the protozoan parasite Leishmania infantum contain antibodies that specifically react with the parasite histone H3. Using...