J. Stévenin

The orderly splicing of the first three leaders of the adenovirus-2 major late transcript (1982)

Keohavong, P., Gattoni, R., LeMoullec, J.M., Jacob, M., Stévenin, J.

A strategy based on the hybridization of labeled nucleoplasmic RNA to a short cloned cDNA probe was devised to study the ligation of the three first leader sequences (Le1, Le2, Le3) of the major late...

SC35 autoregulates its expression by promoting splicing events that destabilize its mRNAs

Sureau, A., Gattoni, R., Dooghe, Y., Stévenin, J., Soret, J.

SC35 belongs to the family of SR proteins that regulate alternative splicing in a concentration-dependent manner in vitro and in vivo. We previously reported that SC35 is expressed through...

The human hnRNP-M proteins: structure and relation with early heat shock-induced splicing arrest and chromosome mapping.

Gattoni, R, Mahé, D, Mähl, P, Fischer, N, Mattei, M G, Stévenin, J, ...

With anti-hnRNP monoclonal antibody 6D12 we previously showed in HeLa cells that as early as 10 min after the onset of a heat shock at 45 degrees C, a 72.5-74 kDa antigen doublet leaves the hnRNPs...

The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins.

Funke, B, Zuleger, B, Benavente, R, Schuster, T, Goller, M, Stévenin, J, ...

The murine poly(C)-binding protein (mCBP) was previously shown to belong to the group of K-homology (KH) proteins by virtue of its homology to hnRNP-K. We have isolated cDNA-splice variants of mCBP...

The different intron 2 species excised in vivo from the E2A premRNA of adenovirus-2: an approach to analyse alternative splicing.

Keohavong, P, Gattoni, R, Schmitt, P, Stévenin, J

In the early period of cellular infection by adenovirus 2, the E2A region gives rise to 2 major mRNA species of 2.0 and 2.3 kilobases, formed by alternative excisions of intron 2 (Gattoni et al.,...

The orderly splicing of the first three leaders of the adenovirus-2 major late transcript.

Keohavong, P, Gattoni, R, LeMoullec, J M, Jacob, M, Stévenin, J

A strategy based on the hybridization of labeled nucleoplasmic RNA to a short cloned cDNA probe was devised to study the ligation of the three first leader sequences (Le1, Le2, Le3) of the major late...

Differential block of U small nuclear ribonucleoprotein particle interactions during in vitro splicing of adenovirus E1A transcripts containing abnormally short introns.

Himmelspach, M, Gattoni, R, Gerst, C, Chebli, K, Stévenin, J

We have studied the consequences of decreasing the donor site-branch site distance on splicing factor-splice site interactions by analyzing alternative splicing of adenovirus E1A pre-mRNAs in vitro....

Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.

Cavaloc, Y, Popielarz, M, Fuchs, J P, Gattoni, R, Stévenin, J

By adopting a monoclonal antibody approach, we have identified a novel splicing factor of 35 kDa which we have termed 9G8. The isolation and characterization of cDNA clones indicate that 9G8 is a...

The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.

Gallego, M E, Gattoni, R, Stévenin, J, Marie, J, Expert-Bezançon, A

Exons 6A and 6B of the chicken beta-tropomyosin gene are mutually exclusive and selected in a tissue-specific manner. Exon 6A is present in non-muscle and smooth muscle cells, while exon 6B is...

Titration of serine/arginine (SR) splicing factors during adenoviral infection modulates E1A pre-mRNA alternative splicing.

Himmelspach, M, Cavaloc, Y, Chebli, K, Stévenin, J, Gattoni, R

Alternative splicing of the adenovirus-2 E1A pre-mRNA involves the use of three 5' splice sites and is modulated during infection because the 13S mRNA and 9S mRNA reactions are predominant during the...

The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.

Cavaloc, Y, Bourgeois, C F, Kister, L, Stévenin, J

The activity of the SR protein family of splicing factors in constitutive or alternative splicing requires direct interactions with the pre-mRNA substrate. Thus it is important to define the high...

SC35 autoregulates its expression by promoting splicing events that destabilize its mRNAs

Sureau, A., Gattoni, R., Dooghe, Y., Stévenin, J., Soret, J.

SC35 belongs to the family of SR proteins that regulate alternative splicing in a concentration-dependent manner in vitro and in vivo. We previously reported that SC35 is expressed through...

The human hnRNP-M proteins: structure and relation with early heat shock-induced splicing arrest and chromosome mapping.

Gattoni, R, Mahé, D, Mähl, P, Fischer, N, Mattei, M G, Stévenin, J, ...

With anti-hnRNP monoclonal antibody 6D12 we previously showed in HeLa cells that as early as 10 min after the onset of a heat shock at 45 degrees C, a 72.5-74 kDa antigen doublet leaves the hnRNPs...

The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins.

Funke, B, Zuleger, B, Benavente, R, Schuster, T, Goller, M, Stévenin, J, ...

The murine poly(C)-binding protein (mCBP) was previously shown to belong to the group of K-homology (KH) proteins by virtue of its homology to hnRNP-K. We have isolated cDNA-splice variants of mCBP...

The different intron 2 species excised in vivo from the E2A premRNA of adenovirus-2: an approach to analyse alternative splicing.

Keohavong, P, Gattoni, R, Schmitt, P, Stévenin, J

In the early period of cellular infection by adenovirus 2, the E2A region gives rise to 2 major mRNA species of 2.0 and 2.3 kilobases, formed by alternative excisions of intron 2 (Gattoni et al.,...

The orderly splicing of the first three leaders of the adenovirus-2 major late transcript.

Keohavong, P, Gattoni, R, LeMoullec, J M, Jacob, M, Stévenin, J

A strategy based on the hybridization of labeled nucleoplasmic RNA to a short cloned cDNA probe was devised to study the ligation of the three first leader sequences (Le1, Le2, Le3) of the major late...

Differential block of U small nuclear ribonucleoprotein particle interactions during in vitro splicing of adenovirus E1A transcripts containing abnormally short introns.

Himmelspach, M, Gattoni, R, Gerst, C, Chebli, K, Stévenin, J

We have studied the consequences of decreasing the donor site-branch site distance on splicing factor-splice site interactions by analyzing alternative splicing of adenovirus E1A pre-mRNAs in vitro....

Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.

Cavaloc, Y, Popielarz, M, Fuchs, J P, Gattoni, R, Stévenin, J

By adopting a monoclonal antibody approach, we have identified a novel splicing factor of 35 kDa which we have termed 9G8. The isolation and characterization of cDNA clones indicate that 9G8 is a...

The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.

Gallego, M E, Gattoni, R, Stévenin, J, Marie, J, Expert-Bezançon, A

Exons 6A and 6B of the chicken beta-tropomyosin gene are mutually exclusive and selected in a tissue-specific manner. Exon 6A is present in non-muscle and smooth muscle cells, while exon 6B is...

Titration of serine/arginine (SR) splicing factors during adenoviral infection modulates E1A pre-mRNA alternative splicing.

Himmelspach, M, Cavaloc, Y, Chebli, K, Stévenin, J, Gattoni, R

Alternative splicing of the adenovirus-2 E1A pre-mRNA involves the use of three 5' splice sites and is modulated during infection because the 13S mRNA and 9S mRNA reactions are predominant during the...

The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.

Cavaloc, Y, Bourgeois, C F, Kister, L, Stévenin, J

The activity of the SR protein family of splicing factors in constitutive or alternative splicing requires direct interactions with the pre-mRNA substrate. Thus it is important to define the high...